The Effect of Edible Coating with Alginate and Shirazi Thyme Essential Oil on the Qualitative Characteristics of Ber Fruit (Ziziphus mauritiana Lam.) during Storage

Document Type : Research Article

Authors

• Aliehsadat Rafaathaghighi
• Leila Jafari
• Abdolmajid Mirzaalian Dastjerdi
• Farzin Abdollahi

Department of Horticulture, University of Hormozgan, Bandar Abbas, Iran

https://doi.org/10.22067/ifstrj.2024.85804.1301

Abstract

Introduction
Ziziphus mauritiana, also known as Ber fruit, is a tropical fruit from the Rhamnaceae family with high nutritional value and a short shelf life. Edible coatings the surface of the fruit can play a positive role in increasing its shelf life. The application of alginate (Al), a linear polysaccharide derived from brown seaweed (Macrocystis pyrifera), which has the advantage of moisture retention, and also Shirazi thyme (Zataria multiflora) essential oil (ZEO), which contains antioxidant and antimicrobial compounds, can improve the fruit's characteristics and preserve its quality for a longer period. In recent years, the application of ZEO together with organic coatings in order to enhance the quality characteristics of different fruits during storage has been the focus of researchers. This study aims to investigate the effect of alginate coating and ZEO on the post-harvest and physicochemical characteristics of Ber fruit during storage at 6±1 C˚ and a relative humidity of 90-95%.
 
Material and Methods
This study was conducted to enhance the shelf life of Ber fruits by treating them with alginate (Al) and Shirazi thyme essential oil (ZEO). Ber fruits at their mature green stage were harvested in 2022 from a commercial orchard in Minab, Hormozgan province. After harvesting, the fruits were transported to the Horticulture Laboratory University of Hormozgan, to apply the treatments. Factors include six treatments (control, Shirazi thyme essential oil 0.5%, Shirazi thyme essential oil 1%, alginate, alginate + Shirazi thyme essential oil 0.5% and alginate + Shirazi thyme essential oil 1%) and five storage periods(zero, 7, 14, 21, 28 days). The treated fruits were then stored at 6±1 C˚, and relative humidity of 90-95% for 28 days. The factorial experiment was conducted as a completely randomized design with four replications, and ten fruits were used in each replication. Fruit parameters such as fruit weight loss, respiration rate, total soluble solids (TSS), organic acid, ascorbic acid, fruit firmness, carotenoids, and chlorophyll content were evaluated. Statistical analysis of data was done using SAS software. Figures were drawn using Excel software.
 
Results and Discussion
Based on the results of the analysis of variance, the main effects of time and treatment were significant (P<0.01) for all characteristics. Additionally, the interaction effect of treatment and time was significant (P<0.01) for all traits except for ascorbic acid. Comparisons of means showed that all treatments, maintained fruit quality compared to the control. The Al + ZEO treatment had a significant effect on reducing respiration rate. This combined treatment reduced weight loss, decay percentage, and fruit softening while slowing down the increase in TSS. The alginate coating and Shirazi thyme essential oil delayed ripening and subsequent fruit softening by reducing ethylene activity. Furthermore, the coated fruits had higher levels of ascorbic acid compared to the untreated fruits. These coatings are impermeable to water vapor and create a saturated atmosphere, preventing fruit evaporation and wrinkling, thus delaying ripening. The use of essential oils in the form of a semi-permeable layer also reduces respiration and surface transpiration by inhibiting the movement of oxygen, carbon dioxide, and moisture, playing a positive role in preserving fruit quality.
 
Conclusion
The aim of this study was to investigate the effect of alginate coating and Shirazi thyme essential oil on the post-harvest and physicochemical characteristics, as well as the shelf life of Ber fruit. The results clearly indicated the enhancement of fruit quality using edible coatings compared to the control. The combination of Al + ZEO (0.5%) had significantly positive effects on preserving the postharvest quality of Ber fruit. In fact, by creating a semi-permeable coating on the surface of the fruit, thyme essential oil and alginate lead to limiting the fruit respiration and metabolism and fungi growth, thus delaying the ripening process, increasing TSS and ascorbic acid and total acidity. According to these results, to reduce post- harvest losses and increase productivity of fruit, the use of 0.5% Shirazi thyme essential oil in combination with alginate coating is recommended due to its beneficial effects on the appearance and physicochemical characteristics of Ber fruit during storage.
 
Acknowledgement
The authors of this article express their gratitude and appreciation for the financial support from the Hormozgan Science and Technology Park and the University of Hormozgan.

Keywords

• Ber fruit
• Edible coating
• Fruit quality
• Respiration
• Storage

Main Subjects

• Food Technology

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

References

1. Abad Ullah, N.A., Abbasi, M., & Qureshi A.A. (2017). Influence of edible coatings on biochemical fruit quality and storage life of bell pepper cv. (Yolo Wonder). Journal of Food Quality, 10(2), 1-11. https://doi.org/10.1155/2017/2142409
2. Ahmadzadeh Ghavidel, R., Gheiafe davoodi, M., Tanoori, T., & Sheikholeslami, Z. (2011). Investigating the effect of four edible coatings of soy protein isolate, whey protein concentrate, carrageenan and alginate in increasing shelf life of pear slices (Pyrus communis). Journal of Innovation in Food Science and Technology, 4(3), 47-54.
3. Ali, A., Maqbool, M., Ramachandran, S., & Alderson, P.G. (2010). Gum arabic as a novel edible coating for enhancing shelf-life and improving postharvest quality of tomato (Solanum lycopersicum) fruit. Postharvest Biology and Technology, 58(1), 42-47. https://doi.org/10.1016/j.postharvbio.2010.05.005
4. Ameh, B.A., Gernah, D.I., Obioha, O., & Ekuli, G.K. (2015), Production, quality evaluation and sensory acceptability of mixed fruit juice from Pawpaw and Lime. Food and Nutrition Sciences, 06(05), 532-537. https://doi.org/10.4236/fns.2015.65055
5. Azarakhsh, N., Osman, A., Ghazali, H.M., Tan, C.P., & Adzahan, N.M. (2014). Lemongrass essential oil incorporated into alginate-based edible coating for shelf-life extension and quality retention of fresh-cut pineapple. Postharvest Biology and Technology, 88, 1-7. https://doi.org/10.1016/j.postharvbio.2013.09.004
6. Bonilla, J., Atares, L., Vargas, M., & Chiralt, A. (2012). Effect of essential oils and omogenization conditions on properties of chitosan-based films. Food Hydrocolloids, 26, 9-16. https://doi.org/10.1016/j.foodhyd.2011.03.015
7. Choi, W.S., Singh, S., & Lee, Y.S. (2016). Characterization of edible film containing essential oils in hydroxypropyl methylcellulose and its effect on quality attributes of ‘Formosa’plum (Prunus salicina). LWT-Food Science and Technology, 70(4), 213–222. https://doi.org/10.1016/j.lwt.2016.02.036
8. Dashipour, A., Razavilar, V., Hosseini, H., Shojaee-Aliabadi, S., German, J.B., & Ghanati, K. (2015). Antioxidant and antimicrobial carboxymethyl cellulose films containing Zataria multiflora essential oil. International Journal of Biological Macromolecules, 72, 606–613. https://doi.org/10.1016/j.ijbiomac.2014.09.006
9. Ehteshami, S., Dastjerdi, A.M., Ramezanian, A., Etemadipoor, R., Abdollahi, F., & Salari, M. (2021). Effects of edible alginate coating enriched with organic acids on quality of mango fruit during storage. Journal of Food Measurement and Characterization, 16, 400-409. https://dio.org/10.1007/s11694-021-01166-4
10. Eshghi, S., Hashemi, M., Mohammadi, A., Badie, F., Mohammad Hosseini, Z., Ahmadi, S.K., & Ghanati, K. (2014). Effect of nano-emulsion coating containing chitosan on storability and qualitative characteristics of strawberries after picking. Iranian Journal of Nutrition Sciences and Food Technology, 8(2), 9-19.
11. Etemadipoor, R., Dastjerdi, A.M., Ramezanian, A., & Ehteshami S. (2020). Ameliorative effect of gum arabic, oleic acid and/or cinnamon essential oil on chilling injury and quality loss of guava fruit. Scientia Horticulturae, 266, 109255. https://doi.org/10.1016/j.scienta.2020.109255
12. Etemadipoor, R., Ramezanian, A., Dastjerdi, A.M., & Shamili, M. (2019). The potential of gum arabic enriched with cinnamon essential oil for improving the qualitative characteristics and storability of guava (Psidium guajava) fruit. Scientia Horticulturae, 251, 101–107. https://doi.org/10.1016/j.scienta.2019.03.021
13. Fan, F., Tao, N., Jia, L., & He, X. (2014). Use of citral incorporated in postharvest wax of citrus fruit as a botanical fungicide against Penicillium digitatum. Postharvest Biology and Technology, 90, 52-55. https://doi.org/10.1016/j.postharvbio.2013.12.005
14. Fan, Y., Xu, Y., Wang, D., Zhang, L., Sun, J., Sun, L., & Zhang, B. (2009). Effect of alginate coating combined with yeast antagonist on strawberry (Fragaria× ananassa) preservation quality. Postharvest Biology and Technology, 53(1), 84-89. https://doi.org/10.1016/j.postharvbio.2013.12.005
15. Garcia, L.C., Pereira, L.M., Sarantópoulos, L., Claire, I.G., & Hubinger, M.D. (2012). Effect of antimicrobial starch edible coating on shelf-life of fresh strawberries. Packaging Technology and Science, 25(7), 413-425. https://doi.org/10.1002/pts.987
16. Ghasemi, M., Ramin, A.A., & Amini, F. (2016). The effect of coating containing chitosan on quality and Increase post-harvest life cucumber cv zomorod. Journal of Production and Processing of Crop and Gardening, 5(15). http://doi.org/10.18869/acadpub.jcpp.5.15.189
17. Gol, N.B., Patel, P.R., & Rao, T.R. (2013). Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan. Postharvest Biology and Technology, 85, 185-195. https://doi.org/10.1016/j.postharvbio.2013.06.008
18. Guerreiro, A.C., Gago, C.M., Faleiro, M.L., Miguel, M.G., & Antunes, M.D. (2015). The use of polysaccharide-based edible coatings enriched with essential oils to improve shelf-life of strawberries. Postharvest Biology and Technology, 110, 51-60. https://doi.org/10.1016/j.postharvbio.2015.06.019
19. Guerreiro, A.C., Gago, C.M., Faleiro, M.L., Miguel, M.G., & Antunes, M.D. (2015a). The use of polysaccharide-based edible coatings enriched with essential oils to improve shelf-life of strawberries. Postharvest Biology and Technology, 110, 51-60. https://doi.org/10.1016/j.postharvbio.2015.06.019
20. Gurjar, P.S., Killadi, B., Lenka, J., & Shukla, D.K. (2018). Effect of gum Arabic coatings on physico-chemical and sensory qualities of Guava (Psidium guajava) cv. Shweta. International Journal of Current Microbiology and Applied Sciences, 7(5), 3769-3775. https://doi.org/10.20546/ijcmas.2018.704.424
21. Haq, MA., Alam, M.J., & Hasnain, A. (2013). Gum Cordia: A novel edible coating to increase the shelf life of Chilgoza (Pinus gerardiana). LWT-Food Science and Technology, 50(1), 306-311.
22. Hashemi, M., Dastjerdi, A.M., Mirdehghan, S.H., Shakerardekani, A., & Golding, J.B. (2021a). Incorporation of Zataria multiflora Boiss essential oil into gum Arabic edible coating to maintain the quality properties of fresh in-hull pistachio (Pistacia vera ). Food Packaging and Shelf Life, 30, 0110. https://doi.org/10.1016/j.fpsl.2021.100724
23. Hashemi, M., Dastjerdi, A.M., Shakerardekani, A., & Mirdehghan, S.H. (2021b). Effect of alginate coating enriched with Shirazi thyme essential oil on quality of the fresh pistachio (Pistacia vera). Journal of Food Science and Technology-Mysore, 58, 34-43. https://doi.org/10.1007/s13197-020-04510-6
24. Hashemi, M., Shakerardekani, A., Dastjerdi, A.M., & Mirdehghan, S.H. (2021c). Effect of sodium alginate in combination with Zataria multiflora on phenolic compounds, antioxidant activity, and browning enzymes of fresh in-hull pistachio (Pistacia vera L.). Journal of Food Quality, 3193573. https://doi.org/10.1155/2021/3193573
25. Hernandez-Munoz, P., Almenar, E., Del Valle, V., Velez, D., & Gavara, R. (2008). Effect of chitosan coating combined with postharvest calcium treatment on strawberry (Fragaria× ananassa) quality during refrigerated storage. Food Chemistry, 110(2), 428-435. https://doi.org/10.1016/j.foodchem.2008.02.020
26. Jiang, T., Feng, L., & Zheng, X. (2011). Effect of chitosan coating enriched with thyme oil on postharvest quality and shelf life of shiitake mushroom (Lentinus edodes). Journal of Agricultural and Food Chemistry, 60(1), 188-196. https://doi.org/10.1021/jf202638u
27. Joshi, A.V., Baraiya, N.S., Vyas, P.B., & Rao, T.R. (2017). Gum Ghatti based edible coating emulsion with an additive of clove oil improves the storage life and maintains the quality of Papaya (Carica papaya, cv. Madhu bindu). International Journal of Current Microbiology and Applied Sciences, 6(5), 160-174. https://doi.org/10.20546/ijcmas.2017.605.019
28. Khoshnoudinia, S., Sedaghat, N., & Radmard-Ghadiri, GH.H. (2013). Effect of gelatin edible coating containing antioxidant agents on hardness, and color of roasted pistachio nuts. JRIFST, 2(4), 295-310.
29. Krishna, H., Kumar, L., Haldhar, S.M., Singh, D., & Saro, P.L. (2018). Phenological growth stages of Indian jujube (Ziziphus mauritiana) according to the BBCH scale. Annals of Applied Biology, 174, 106–112. https://doi.org/10.1111/aab.12466
30. Lichtenthaler, H.K., & Buschmann, C. (2001). Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry, 3, 821-828.
    https://doi.org/10.1002/0471142913.faf0403s01
31. Maftoonazad, N., Ramaswamy, H.S., & Marcotte, M. (2008). Shelf-life extension of peaches through sodium alginate and methyl cellulose edible coatings. Food Science and Technology, https://doi.org/10.1111/j.1365-2621.2006.01444.x
32. Martinez, D., Guillen, S., Castillo, S., Valero, D., & Serrano, M. (2003). Modified atmosphere packaging maintains quality of table grapes. Journal Food Science, 68, 1838-1843. https://doi.org/10.1111/j.1365-2621.2003.tb12339.x
33. Meng, X., Li, B., Liu, J. & Tian, S. (2007). Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry, 101, 501-508. https://doi.org/10.1016/j.foodchem.2007.06.012
34. Mi Moon, K., Kwon, E., Lee, B., & Young Kim, C.H. (2020). Recent trends in controlling the enzymatic browning of fruit and vegetable products. Molecules, 25(12), 2754. https://doi.org/10.3390/molecules25122754
35. Moraes, K.S.D., Fagundes, C., Melo, M.C., Andreani, P., & Monteiro, A.R. (2012). Conservation of Williams pear using edible coating with alginate and carrageenan. Food Science and Technology, 32(4), 679-684. https://doi.org/10.1590/S0101-20612012005000106
36. Murmu, S.B., & Mishra, H.N. (2018). Selection of the best active modified atmosphere packaging with ethylene and moisture scavengers to maintain quality of guava during low-temperature storage. Food Chemistry, 253, 55–62. https://doi.org/10.1016/j.foodchem.2018.01.134
37. Nair, M.S., Saxena, & A., Kaur, C. (2018). Effect of chitosan and alginate based coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava). Food Chemistry, 240, 245-252. https://doi.org/10.1016/j.foodchem.2017.07.122
38. Pareek, S., & Yahia, E.M. (2013). Postharvest biology and technology of Ber fruit. Horticulture Reviews, 41(1), 201-240. https://doi.org/10.1002/9781118707418.ch05
39. Pooraziz, S., Nazuri, F., Mirdehghan, S.H., & Esmaeilizadeh, M. (2019). The effect of sodium alginate coating on the shelf life of strawberry (Fragaria ananassa) variety Gaviota. Iranian Journal of Horticultural Science, 50(3), 515-526.
40. Qiuping, Z., & Wenshui X. (2007). Effect of 1-methylcyclopropene and/or chitosan coating treatments on storage life and quality maintenance of Indian jujube fruit. LWT- Food Science and Technology,40(3), 404-411. https://doi.org/10.1016/j.lwt.2006.01.003
41. Raybaudi-Massilia, R.M., Mosqueda-Melgar, J., & Martin-Belloso, O. (2008). Edible alginate-based coating as carrier of antimicrobials to improve shelf-life and safety of fresh-cut melon. International Journal of Food Microbiology, 121(3), 313-327. https://doi.org/10.1016/j.ijfoodmicro.2007.11.010
42. Reche, J., Almansa, M.S., Hernández, F., Amorós, A., & Legua, P. (2021). Physicochemical and antioxidant capacity of jujube (Ziziphus jujuba) at different maturation stages. Agronomy, 11(1), 132. https://doi.org/10.3390/agronomy11010132
43. Rojas-Grau, M.A, Soliva-Fortuny, R., & Martin-Belloso O. (2009). Edible coatings to incorporate active ingredients to fresh-cut fruits. Trends in Food Science and Technology, 20, 438–447. https://doi.org/10.1016/j.tifs.2009.05.002
44. Rojas, Grau., A., Maria, S.T., & Olga, M.B. (2008). Using polysaccharide-based edible coatings to maintain quality of fresh-cut Fuji apples. LWT- Food Science and Technology, 44, 139-147. https://doi.org/10.1016/j.lwt.2007.01.009
45. Rojas-Grau, M.A., Tapia, M.S., Rodriguez, F.J., Carmona, A.J., & Martin-Belloso, O. (2007). Alginate and gellan based edible coatings as support of antibrowning agents applied on fresh-cut Fuji apple. Food Hydrocology, 21, 118−127. https://doi.org/10.1016/j.foodhyd.2006.03.001
46. Saltveit, M.E. (2005). Postharvest biology and handling, in: Heuvelink, E. Ed., Tomatoes. CAB International, Wallingford, 305-324.
47. Siripatrawan, U., & Harte, B.R. (2010). Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocolloids, 24(8), 770–775. https://doi.org/10.1016/j.foodhyd.2010.04.003
48. Sogvar, O.B., Saba, MK., & Emamifar, A. (2016). Aloe vera and ascorbic acid coatings maintain postharvest 'quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology, 114, 29-35. https://doi.org/10.1016/j.postharvbio.2015.11.019
49. Tanada-Palmu, P., & Grosso, C. (2005). Effect of edible wheat gluten-based films and coatings on refrigerated strawberry (Fragaria ananassa) quality. Postharvest Biology and Technology, 36, 199–208. https://doi.org/10.1016/j.postharvbio.2004.12.003
50. Thumula, P. (2006). Studies on storage behaviour of tomatoes coated with chitosan-lysozyme films. M.Sc. thesis. Department of Bioresource Engineering Faculty of Agricultural and Environmental Sciences. McGill University Montreal, Quebec, Canada, 128 pp.
51. Valero, D., Diaz-Mula, H.M., Zapata, P.J., Guillen, F., Martinez-Romero, D., Castillo, S., & Serrano, M. (2013). Effects of alginate edible coating on preserving fruit quality in four plum cultivars during postharvest storage. Postharvest Biology and Technology, 77, 1-6. https://doi.org/10.1016/j.postharvbio.2012.10.011
52. Valiathan, S., & Athmaselvi, K.A. (2018). Gum arabic based composite edible coating on green chillies. International Agrophysics, 32(2), 193-202. https://doi.org/10.1515/intag-2017-0003
53. Vishwasrao, Ch., & Laxmi, A. (2016). Postharvest shelf-life extension of pink guavas (Psidium guajava) using HPMC-based edible surface coatings. Journal of Food Science and Technology, 53, 1966-1974. https://doi.org/10.1007/s13197-015-2164-x
54. Wang, K.T., Jin, P., Cao, S.F., Shang, H.T., Yang, Z.F., & Zheng, Y.H. (2009). Methyl jasmonate reduces decay and enhances antioxidant capacity in Chinese bayberries. Journal of Agricultural and Food Chemistry, 57, 5809–5815. https://doi.org/10.1021/jf900914a
55. Wang, S.Y., & Gao, H. (2013). Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x aranassa ). Food Science and Technology, 52(2), 71-79. https://doi.org/10.1016/j.lwt.2012.05.003
56. Wittaya, T. (2012). Protein-based edible films: Characteristics and improvement of properties. In Structure and Function of Food Engineering. InTech.
57. Xing, Y., Li, X., Xu, Q., Yun, J., Lu, Y., & Tang, Y. (2011). Effects of chitosan coating enriched with cinnamon oil on qualitative properties of sweet pepper (Capsicum annuum). Food Chemistry, 124(4), 1443-1450. https://doi.org/10.1016/j.foodchem.2010.07.105
58. Zheng, H., Liu, W., Liu, S., Liu, C., & Zheng, L. (2019). Effects of melatonin treatment on the enzymatic browning and nutritional quality of fresh-cut pear fruit. Food Chemistry, 299, 1-18. https://doi.org/10.1016/j.foodchem.2019.125116