Iranian Food Science and Technology Research Journal

Abstract In this study,The effect of Ozonated water(0.2, 0.5, and 1 ppm) with different exposure times (5,10, and 15 min) for control of Staphylococcus aureus , Escherichia coli and total counts in poultry Carcass, on Chiller water in slaughter house were investigated. For this purpose, Control samples passed chiller water(without Ozone) for (5,10, and 15 min) .Other examples also treated (5, 10 , and 15 min) with ozonated water(0.2, 0.5, and 1 ppm) in the laboratory, under the same temperature of chiller water(0 - 4 C) and immediately analyzed for microbiological tests. The results indicated that the use of ozonated water redused1.7 Log of Staphylococcusaureus , 2.3 Log of Escherichia coli and 1.16 Log of total counts. Results showed that Escherichia Coli (As a Gram- Negative Food Infection Bacteria) was more sensitive to Ozone treatment than Staphylococcus aureus(As a Gram-Positive Food Infection and Toxicogenic Bacteria),(2.3 Log reduction in Escherichia coli and 1.7 Logreduction in Staphylococcus aureus), At all levels observed significant differences (P

Abstract This research aims to assess the physicochemical properties, image features, textural and sensory qualities of dark chocolate made using coffee silver skin as a cocoa powder replacement which was substituted for 25%, 50%, 75% and 100% of the cocoa powder (by weight) in chocolates. The results indicated that there was significant differences between the chocolates prepared using 100% coffee skin and the control, for moisture content (p

Abstract In this research, a mixture of MPC, WPC, SMP, Soy milk, Margarine, Butter and water was used for production of UF-Feta cheese analogue.Variables were MPC (8%, 9%, 10%), WPC (0%, 1.5%, 3%), soy milk (5%, 10%, 15%) and margarine (0%, 5%, 10%). Chemical (total solids, protein, acidity) and sensory (color and appearance, aroma, texture, flavor, total acceptance) properties of Samples were analyzed 3 days post- manufacture.The central composite design (CCD) was employedand the results were analyzedusing response surface methodology (RSM). Coefficients of determination, R2, of fitted regression models for mentioned variables ranged between 84.94-99.99 and the lack-of-fit was not significant for all response at 95%. Hence, the models for all the response variables were highly adequate.The results showed that the optimum processing conditions for producing cheese with standard total solids and protein content and highest overall values for the sensory properties were: 9.13% MPC, 3% WPC, 15% soy milk and 7.65% margarine.

Abstract In this study, quality of white button mushrooms (Agaricus bisporus) treated by water and citric acid was evaluated during storage at 2 temperatures (room temperature and 4°C). The effect of modified atmosphere packaging (MAP, by 4±1% CO2, 6±1% O2 and the remaining N2) with or without water absorbent (silicagel) was also evaluated. Samples were assessed for their organoleptic properties (texture, color and spoilage), colorimetery and respiration rate during 12 days of storage. Results showed that among all treatments, water treated samples packaged with MAP method contained silicagel sachet and also stored at refrigerator obtained the most organoleptis scores. This sample retained the optimum level of inter packaging gas composition and finally the best storage time was determined as 8 days.

Abstract Introduction: Meat quality is always very important for consumers. Tenderize meat one way to improve the quality of Meat and its products. In the study effect of three levels (0.0025, 0.005 and 0.01 percent) of protease produced by Aspergillus niger on meat tenderness during the 28 days of storage at 4 ° C were studied. Then treatments to control the physicochemical and sensory characteristics were evaluated. In this study tests the total soluble protein, moisture content, pH, colorimetric, texture analysis, water holding capacity and sensory evaluation of meat quality parameters were used. The results of the tests carried out showed that soluble proteins, pH and moisture content levels generated by increasing the enzyme from 0.0025 to 0.01 percent was significantly increased (P<0.05), But on the rigidity by increasing the enzyme decreased firmness. Results also showed that water holding capacity has decreased, but all treatments during storage period 0/01 enzyme treatment showed the highest capacity at the end. Brightness index (L *) and yellowness index (b *) during maintenance treatments reduce the redness index (a *) increased. The sensory characteristics of diet supplemented with 0.0025% of fungal enzymes and control the admission showed more acceptable. So this study using Aspergillus niger enzyme for use in to tenderize meat it considers appropriate.   Materials and methods: For this purpose, 4 samples of 500 grams of Rhomboideus muscle from the 1.5-year-old Holstein male calf from the Rock Company slaughterhouse were immediately prepared for killing. Samples were stored in a refrigerator at 4 ° C for 48 to 72 hours. Each muscle was maintained as a non-injectable control unit (control sample). Then injected into three other parts of the enzyme solution. Protein solution of Aspergillus Niger was obtained from the Iranian Institute of Science and Technology. Then injection of the extracts of the enzyme into the sequences by the injector with 0.01% (AN 0/01), 0.005% (AN 0/005) and 0.0025% (AN 0/0025) (Mg / 100 grams of meat) of fungal protease separately into meat pieces. The pieces of meat were treated using an injector with 4 needles in each row with 10 needles with a spacing of 2.8 cm and a pressure of 35 injections. After injection, the muscle samples were packed under vacuum and immediately stored at 4 ° C for 28 days. On the days 1, 7, 14, 21 and 28, the relevant tests were performed on the treatments. To measure the pH, 10 g of the milled meat was mixed in 90 g of deionised water. The prepared mixture was then plated with a rough filter paper (average watten-diameter of 150 mm). Finally, pH was measured using a digital pH meter. The homogeneous moisture content of the meat samples was measured using the oven method and according to AOAC standard No. 46/950. The basis of the test is based on the measurement of total nitrogen in foods, assuming that all N is a protein type and is based on the coefficients of N-to-protein conversion. To determine the protein values, use the method outlined in AOAC (1996). Used. To measure the protein, the Kjeldahl method was used by the Kjeldahl machine constructed in Japan. The color of the meat was measured by a color spectrometer (CR-300, Minolta, Co. Ltd., Japan) every seven days. White tile with b *: 1.72; a *: -0.02; L *: 97.46 is considered as reference.This test was performed to examine the tissue texture strength of the specimens by using Feismann et al. (1999) by Brookfield Engineering Laboratories, USA at 4 ± 2 ° C. In this test, a cylindrical probe with a smooth cross-section with a diameter of 12.7 mm and a speed of 1 ml / sec was used. In order to measure the water holding capacity, the amount of water extracted from the cutting surface of meat samples under 500 psi pressure and 1 minute time to the filter cassette was accurately weighed to 0.001 g and was expressed as extractable moisture content. The evaluation of sensory features in terms of five factors including hardship, substrate, texture, color and general acceptance was performed using 10 evaluated trainees by Hedonic method by completing an evaluation questionnaire. To each of the mentioned factors, the privilege was allocated from 1 to 5. The way to score was based on the fact that the number 5 represents the highest score and the number 1 represents the lowest score all experiments were performed in a completely randomized design with three replications. Means were compared using SPSS 21 and based on Duncan's tests at 5% level. The resulting charts in the 2013 excel software were drafted and compared.   Results & Discussion: There are several ways to improve the quality of meat and its better use in the production of meat products. One of these methods is to increase the solubility of the meat proteins and, consequently, to crumble and increase the properties of the emulsion and other functional properties such as increasing water holding capacity. Today the effects of the enzymes in plants, bacteria and fungi have been investigated. In this study, the Aspergillus Niger fungal enzyme was used to crust meat. One of the results of this study is that the fungal enzymes have been able to greatly increase the water content of the water, pH and soluble proteins in the flesh, due to increasing the duration of the enzyme's effect and the concentration of the enzyme on Insoluble proteins such as collagen and elastin are completely digestible and their nature has changed (Englund et al., 1968). Also, the results show that the enzyme breaks down some of the protein proteins and forms them in smaller molecular weight units. As you can see, increasing the amount of enzyme concentration on meat proteins has increased the amount of crust and solubility of meat proteins.

Abstract Introduction: Using oilseeds in the human food stuffs, employing their meal for animal feed and also their usage in pharmaceuticals, soap making and fuel has prompted great interest for farmers to plant them and for the government to promote their cultivation. Among them, sunflower is one of the main oilseeds in the world which its cultivated area has expanded due to fair cultivation requirements, high yield of the oil, high nutritional value and also lack of anti nutritional factors. Sunflower (Helianthus annuus) is an annual plant belonging to Asteraceae family. This is a dicotyledonous, cross-pollinated monoecious plant that is fertilized by wind and insects. Sunflower seed oil has an excellent nutritional quality, as in recent years, cultivars with high oil (especially oleic acid) content have been substantially nurtured. The most different methods of extracting oil from oilseeds are the press and solvent methods. Similar to the other seeds with high oil content such as canola, the most effective way of extracting oil from sunflower is mechanical pressing followed by solvent extracting. In this method, the mechanical press extracts about 60 percent of the oil and the solvent method extracts the remaining oil. For the first time, the present study was aimed to improve temperature of cooker and moisture of output seeds for producing sunflower oil with lowest degree of insoluble fine partial in oil, moisture and acidity and meal with lowest levels of moisture and oil. Materials and Methods: Sunflower seeds used in this research were supplied from one of Iran's provinces and were transferred to the company of Khorasan cotton and oilseeds to produce oil and meal. After receiving the sunflower seeds in the factory, they were entered into silos in dark and ambient temperature; impurities such as dust, sands, stones, spoiled seeds, small weed seeds and other extraneous materials were separated by mechanical sieves. After cleaning, the seeds were entered into the cracker and they were broken into smaller particles and then were moved into the cooker; at this stage, the temperature of cooker and moisture content of the exiting seeds were set to 70, 80 and 900 C, and 7, 7.5 and 8%, respectively. Then, conditioned seeds were entered into the Buhler flicker device for flaking. Afterwards, the flakes were moved into the Desmet extractor (heating condition of 500C for 7 hours) to extract the oil from the seeds by hexane solvent. Then, the tests were performed on the oil and meal. Severalphysic-chemical properties of sunflower oil including insoluble fine partial, acidity values as well as moisture, protein and oil contents of the obtained meals were determined. Statistical analysis and process optimization were carried out using response surface methodology (RSM). Results and discussion: The achieved results expressed that with an increase in cooking temperature, insoluble fine partial and oil acidity values of the extracted oil were boosted while moisture content of oil and meal values alongside oil content of the obtained meal showed reduction. With increasing of the moisture content of cooker’s seeds, the insoluble fine partial value of the extracted oil was reduced while oil acidity value was increased. Increasing the moisture of cooker’sseeds led to the oil reduction in the meal. The highest oil content in the meal was achieved in the condition that the cooker temperature was 70oC and the moisture of output seeds from the cooker was 7%. The analysis of resulted data showed that two parameters of the cooker’s temperature and cooker’s seeds moisture content had significant effects on the moisture content of the meal. Increasing the cooker temperature from 70 to 90oC caused a decrease in the meal moisture. As result shown, increasing the moisture content of output seeds from the cooker increased the moisture content of the meal. Increasing the cooker temperature from 70 to 90oC reduced the protein amount of the meals. Results of different studies showed that increasing the temperature will decrease the protein amount of the meals. Increasing the moisture was also resulted in the decrease of residual protein in the meal. The obtained results of the optimization procedure revealed that the application of the cooking temperature of 70 °C and moisture content of the output seeds equal 7.73 and 7.65 % led to achieving products with the least values of acidity and insoluble fine partial in the obtained oil as well as meals with the minimum remaining oil.

Abstract Introduction: Natural polymers have gained increasing attention for the development of packaging to reduce ecologically-related problems caused by plastic packaging (environmental pollution). Among these natural polymers, proteins such as gelatin is considered a good candidate for food packaging. However, several studies have shown that gelatin films are brittle, and their hydrophilic nature connotes high water vapor permeability and water solubility. Different solutions have been suggested to overcome these weaknesses, including adding crosslinking agents and chemical modification, adding nanoparticles and developing bio-film blends and bilayers with polysaccharides. Agar is a polysaccharide extracted from marine red algae, which is biocompatible, has good mechanical properties and possesses good film-forming properties. Preventing food spoilage from light and oxygen-induced oxidation is one of the greatest concerns in the food industry. Despite having good mechanical and relatively good water vapor permeability properties, protein- or polysaccharide-based films don't have sufficient barrier properties against oxygen and UV light that can't properly prevent the oxidation of food products. Among nanoparticles, metal oxides like TiO2 (such as antiradiation and antimicrobial activities) and Montmorillonite (such as improved mechanical and barrier properties against moisture, WVP and gases) have evidenced good potential to improve functional properties of bio-films. Thus, the present study aimed to develop a new biodegradable bilayer agar/gelatin film incorporating nanoclay and TiO2 for food packaging, with maximum water sensitivity and maximum UV light and oxygen barrier properties. Materials and Methods: Agar/gelatin bilayer films were prepared by a two-step casting technique. First, the agar layer was produced by solubilization of 1.5 g of agar powder (agar-agar analytical grade were obtained from Merck Co., Germany) in 100 mL of distilled water. Then, glycerol (obtained from Merck Co., Germany) was added as plasticizer. The agar film-forming solution was casted onto petri-dish. In the next step, the gelatin (obtained from cold water fish skin was purchased from Sigma-Aldrich, St. Louis, MO, USA) solutions were prepared by dissolving 4 g of the fish gelatin in 100 mL of distilled water. Glycerol was also added as plasticizer. The TiO2 dispersions (in ratios of 0 and 2% of the gelatin) and MMT (Na+–montmorillonite (in ratios of 0, 3, 5 and 10% of the gelatin)) were added to the gelatin solution and stirred and sonnicated. Finally, produced solutions were then casted. The agar/gelatin films, with or without TiO2 and MMT, were characterized using SEM analysis. Film transparency, water vapor permeability, water solubility, swelling, surface color and mechanical properties of the bilayer films were also examined. Results and Discussion: In this study, bilayer films based on agar and gelatin incorporated with TiO2-MMT nanoparticles have been successfully developed. Results demonstrated that some properties of the bilayer films were greatly influenced by TiO2 and MMT nanoparticle content. So that, the addition of TiO2 decreased water vapor permeability of the bilayers more than 15%, upon increasing TiO2 content to 2%. However, swelling ratio and moisture content increased with the increase in the nano-TiO2 content, probably due to the hydrophilic nature of the TiO2 nanoparticles. Also, whiteness index (WI) increased by adding 2% of TiO2 nanoparticles. As shown in the surface photograph of the bilayer films, TiO2 generated more opaque and whiter films, which might be related to the white color of TiO2 nanoparticles in solution form. Also, the addition of MMT (0, 3, 5, and 10%) to bilayer-2% TiO2 significantly decreased water vapor permeability and transmission of UV light of the bilayer films. However, tensile strength (TS) decreased with further increase of the nanoparticle concentration. Increasing the concentration of nanoparticles' MMT to 5%, the tensile TS of the agar/gelatin films increased from 12.86 to 20.54 MPa; it might also be related to the interactions between sulphydryl and carboxylic groups from certain amino acids in the gelatin structure with MMT and TiO2 nanoparticles. However, the TS decreased again with further increase of the filler content up to 10% MMT. Also, the addition of MMT from 3%-10% concentration significantly reduced the elongation at break value (EB) of the bilayer films from 41.77 to 28.90% for the bilayer films (p

Abstract Introduction: Tomato is one of the most valuable sources of minerals and vitamins supply in the human diet. Low shelf life of tomato and its short shelf life with inadequate processing facilities lead to heavy financial losses. Therefore, preserving and processing of tomato are of the commercial importance. Drying is one of the preferred methods for tomato preservation. Dried tomato products including half tomatoes, tomato slices and tomato powder, have many consumptions, compared with other tomato products. Among dried tomato products, tomato powder has a particular market. Powder production is an alternative method to extend the shelf life of foods. Usually the fruit powder is very dry, humidity absorber and has too much volume. Therefore, during storage, transportation and administration, it requires special care and heavy packaging which increase the cost. To overcome these problems, compression of the fruit powder in tablet form could be a proper solution. Tablet making of fruit powder has gained much popularity due to its ease of use, storage, transportation and product formulation. After the tablet making process, the pills contain high moisture contentwhich makes them un-suitable for transportation and storage. So, in order to prevent the tablets corruption and maintain their quality, tablet drying is one of the important steps after the tablet making process. The drying process is an important operation that affects the quality and final price of the product. Different drying methods play an important role in protecting foodstuffs. However, the effect of different drying methods on the quality of some foodstuffs is not clear.
 
Materials and methods: In this research, after the preparation of fresh tomatoes, the primary moisture content of tomatoes was determined using hot air oven method. Then, using blanching method, the tomatoes were peeled and samples were cut using a sharp razor in thicknesses of 3 mm. Tomato slices were dried using a semi-industrial dryer in a hot air at 50 °C at an air speed of 1 m/s. Dried tomato slices were powdered using a grinder. In order to homogenize the particle size, the tomato powder was sieved by a 50 mesh (Cavity size 0.5 mm) sample. Suitable moisture content to create sufficient adhesion between the particles of tomato powder was selected at %23 d.b. Water and fructose were used as a bonding agent. The process of producing tomato spherical tablets was performed by a hydraulic press. Drying tests of wet compressed tablets were performed immediately after the end of the tabletting process using a hot air drying machine with microwave pre-treatment. In this study, the effect of drying variables,through using of  microwave pre-treatment along with hot air, including air temperature in five levels (40, 50, 60, 70 and 80 °C), air velocity at five levels (0.5, 1, 1.5, 2, and 2.5 m/s) and the duration of microwave application at five levels (zero (without microwave), 4, 8, 12, and 16 s) on physical properties (shrinkage and unit density), mechanical (penetration resistance) and thermal (Effective moisture diffusivity and energy consuming drying) of compact pomegranate produced from tomato powder were studied. Statistical analysis of data and optimization of drying process were performed using response surface method and central composite design.
 
Results and discussion: Results showed that effect of air temperature and duration of microwave pretreatment on all variables of compressed tomato tablets were significant in hot air dryers with microwave pre-treatment. Increasing temperature and time of microwave pre-treatment increased the effective moisture diffusivity and shrinkage of the final product. The temperature of the air inlet to the dryer had a negative effect on the resistance to penetration, the unit density and the specific energy consumption of the drying process. Drying under lower temperature conditions and less time for microwave pre-treatment resulted in an increase in the desirability of the drying process

Abstract Introduction: Recently, the prevalence of diseases related to diabetes, hypertension, and obesity, due to high and persistent consumption of sucrose is increasing rapidly. Concerns about the adverse effects of sugar consumptionon one hand, and manufacturers and consumers attention for natural sweeteners to replace sugar, on the other hand, reveal that innovative approaches are needed to help develop a healthy diet which prevents such diseases. Stevia can be introduced as a good replacement for sucrose. Corchorus olitorius L. is a rich source of bioactive compounds such as protein, fiber, vitamins, minerals, sterols, tocopherols, and carotenoids, which has significant antioxidant, antibacterial, antifungal, antiviral, anti-inflammatory and anti-cancer properties. This study aimed to reduce the sugar level in Luz preparation and produced a high nutritive product by using Corchorus olitorius.
 
Materials and methods: Materials used in Luz formulation consisted of isomalt, sorbitol, coconut powder, pistachio powder, cardamom, cinnamon and chlorophyll, stevia, and Corchorus olitorius were supplied from Salamat-gostaran arayan Co. All chemicals were from Merck Co. The formulations of Luz include sorbitol (29.68%), sugar (25.85%), coconut powder (38.75%), cardamom (0.3%), cinnamon (0.26%) and chlorophyll (58.5%). 2) were selected, and isomalt was used as filler. To produce Luz, the mixture of sorbitol and isomalt was heated to reach boiling temperature and complete dissolution. After the heat treatment (126°C for 1h), the mixture was cooled to 40°C and the other materials were added and mixed. The prepared samples were molded and placed at 15°C for 3 h. Moisture, ash, protein, fat, and sugar were determined according to AACC standard numbers. The density was also determined according to AACC standard number 54-21. Textural properties were determined using a texture analyzer. Atomic Spectroscopic measurement of minerals was performed according to Devatkal et al. (2004). The color evaluation was performed by the Image Processing method. In this study, the effects of Stevia replacement with sugar (0-100%), Corchorus olitorius replacement with pistachio powder (0-100%) and cooking temperature (50-70°C) on physical and textural properties (density, hardness) of Luz were investigated. Formulation optimization of enriched dietary Luz was performed using Response Surface Method in the form of a central composite design with 6 central points and two replications (α=2) in other locations. In optimum condition, the results of physicochemical, textural and color properties were analyzed by SPSS software.
 
Results and discussion: The linear model was suggested for density changes, and independent effect of variables (stevia replacement, Corchorus olitorius replacement, cooking temperature), interaction effect of Stevia and Corchorus olitorius replacement, interaction effect of Corchorus olitorius replacement and cooking temperature, the quadratic effect of Stevia replacement and the quadratic effect of cooking temperature were significant on density (P <0.05 or P <0.001). The density of all treatments increased with increasing the levels of Stevia and Corchorus olitorius replacement. An increase in density was observed with increasing cooking temperature. The interaction effect of Corchorus olitorius and cooking temperature caused a significant (P<0.05), decrease in hardness. A reduction in the level of stevia replacement resulted in a decrease in hardness. The optimum formulation of dietary Luz was introduced containing 25% Stevia, 25% Corchorus olitorius and cooking temperature of 65°C, and 55% Stevia, 75% Corchorus Olitorius and cooking temperature of 55°C. Overall, the nutritional value of dietary Luz containing Corchorus olitorius was improved in terms of protein, ash, magnesium and calcium content. The total sugar content of the optimum samples was significantly lower than the control. The textural characteristics of the optimum samples had not changed significantly.
 

Abstract The effect of sonication time (in three levels 0,2,4 min ) and mono- di glyceride (in three levels 0,0.3,0.6 %) on improving the quality of cupcake were studied. Color analyze (L*, a*,b*) for dough samples and specific volume, porosity shelf life, color analyze and texture of cake were done. Results showed that increasing the levels of each factors (sonication and emulsifier) made a decrease in a*, b* of dough, crust and crumb of cake and increased in porosity, volume and L* of dough, crust and crumb color of cake. While these two factors used together changes were more obviously. In sample contain 0.6 % emulsifier that sonicated for 4 minute the highest decrease in a*, b* and increase in porosity, volume, shelf life and L* were observed.

Abstract Introduction                                                           
There has been an increasing demand for functional analog meat products due to environmental, human health, and animal welfare concerns. Burger analogs are plant-based products that are designed to mimic the taste, texture, and appearance of meat burgers. They are typically made from a combination of plant-based ingredients such as textured vegetable protein, legumes, grains, and vegetables. The goal of burger analogs is to provide a meat-like experience without using animal-based products.These formulations aim to provide a healthier and sustainable alternative to conventional meat products. The organoleptic properties of burger analogs, including texture, taste, and aroma, are crucial for their acceptance by consumers.
Prickly pear (Opuntia stricta) is a fruit from the Cactaceae family that contains various beneficial components, including natural pigments, proteins, fibers, and polysaccharides. Pectic polysaccharides and arabinogalactans are two types of polysaccharides found in prickly pear that have thickening properties and can improve the texture of food products. Moreover, Prickly pear is a nutritious and functional fruit that can provide various benefits when incorporated into the diet or used as an ingredient in food products. To date, no health benefit analog burgers incorporating Opuntia fruit have been developed.This study investigated the effects of adding Prickly pear pulp powder at levels of 0.5-2.5% on the physicochemical, sensory, and textural properties of analog burgers.
Materials and Methods
Analog burgers were formulated according to Iranian national standards using common ingredients (texturized soy protein, water, canola oil, garlic, dehydrated onion, soy sauce, and guar gum) as a control sample. Mature prickly pear fruits (Opuntia stricta) were collected from west of Mazandaran province in February. The  fruits were washed, peeled, and dried in a forced oven dryer at a temperature of 45 °C. The dried samples were then ground into a powder and stored at 4 °C until further physico-chemical parameters of the  including moisture, pH, ash, protein, lipid, color and total phenolic content. For developing new formulation of analog burgers, the roasted flour was substituted with prickly pear pulp powder at 0.5%, 1.5%, and 2.5% of the base recipe. The average moisture, ash, fat, carbohydrate content, pH, holding capacity, and color of each raw packed burger were measured. The hardness, springiness, cohesiveness, and chewiness of cooked analog burgers were evaluated using a texturometer instrument. Sensory analysis was performed by 10 panelists who judged discrimination scales of color, odor, taste, and texture characteristics. Analysis and sample treatments were repeated at least three times. Statistical analysis was performed using SPSS (version 19.0), and data were expressed as means ± standard deviation (SD).
Results and Discussion
The lowest and highest cooking losses were observed in analog burgers with 2.5% pulp powder (21.03 ±0.47%) and the control (22.2 ± 0.63%), respectively. However, moisture retention and juiciness did not show significant differences (p > 0.05) between analog burgers with prickly pear pulp powder and the control. The results indicated that increasing prickly pear pulp powder levels significantly decreased the redness (+a*) parameter and yellowness (+b*) of raw analog burgers. Moreover, a reduction in cooking loss and shrinkage were observed for cooked soy burger samples using prickly pear pulp powder. However, cooked analog burgers with added prickly pear pulp powder showed significantly higher juiciness. The elasticity of the produced analog burgers significantly decreased with an increasing percentage of pulp powder (p <0.05).
Conclusion
The incorporation of prickly pear pulp powder in analog burger formulation resulted in a significant decrease in cooking loss and shrinkage of the cooked burgers, while not significantly affecting moisture retention and juiciness. An increase in prickly pear pulp powder levels in analog burger formulation led to a significant decrease in the redness and yellowness of the raw analog burgers, as well as a decrease in their elasticity. Based on the sensory evaluation and consumers' overall tendency to consume burgers, it is recommended to use 1.5% prickly pear pulp powder in analog burger formulation.

Abstract Introduction
The use of safe ingredients to preserve food is steadily increasing. The high time and cost of production and approval of synthetic food additives and the reduction of public acceptance of these compounds have caused serious problems in their utilization. Excessive use of synthetic preservatives, which some of them are suspected to be toxic, has completely eliminated these additives and led to the use of natural alternatives to preserve or extend the shelf life of food products. Many plant-based bioactive compounds are good alternatives to synthetic antimicrobial and antioxidant supplements. Plant extracts have significant biological activity including antioxidant, antibacterial, and antifungal properties, which has increased their use in food products. In addition, plant-derived antimicrobial compounds have been considered in the pharmaceutical industry to control microbial pathogens. Natural antioxidant and antimicrobial compounds are receiving a lot of research and industrial attention in food preservation technologies. In the last 2 decades, the use of herbal medicines rich in bioactive molecules (including polyphenols, carotenoids and flavonoids) with medicinal and health effects such as delaying the onset of some diseases such as cardiovascular disorders, diabetes, and cancer have increased.
The plant Prosopis farcta grown in arid and semi-arid regions. In Iran, it is found in the southern regions of the country. In traditional medicine, this plant is used to prevent hyperlipidemia and hyperglycemia, to treat hemorrhoids, intestinal diseases and diarrhea, and leprosy, and to reduce abortion. In addition, antimicrobial and antioxidant properties of various species of Prosopis have been reported. Accordingly, in this study, after examining the of total phenols and flavonoids concentrations, the antioxidant and antimicrobial properties of ethanolic extract of Prosopis farcta were determined.
 
Materials and Methods
The ethanolic extract of P. farcta was obtained maceration method. Total phenol content (by Folin-Ciocalteu reagent method), total flavonoid content (by aluminum chloride method), antioxidant activity (by DPPH and ABTS free radical scavenging and beta-carotene bleaching methods), and antimicrobial effect against Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis (by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration) of the extract were evaluated.
 
Results and Discussion

farcta ethanolic extract showed high phenol content (145.58 ± 1.30 mg GAE/g), while its total flavonoid content was 72.37 ± 1.48 mg QE/g. Antioxidant activity of ethanolic extract of melon root using different methods of DPPH and ABTS free radical scavenging and beta-carotene bleaching inhibition were 62.60, 71.82 and 54.50%, respectively. Antibacterial activity of P. farcta ethanolic extract against Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis according to disk diffusion agar and well diffusion agar methods showed that the antimicrobial activity of the extract was concentration dependent and Shigella dysentery and Staphylococcus aureus were the most resistant and sensitive bacterial strains to the extract respectively. The minimum inhibitory concentrations of ethanolic extract of P. farcta root for Escherichia coli, Shigella dysentery, Staphylococcus aureus, and Bacillus subtilis were 8, 8, 4 and 4 mg/ml, respectively; while the minimum bactericidal concentrations for these bacteria were 128, 256, 32 and 64 mg/ml, respectively.

 
Conclusion
In the present study, ethanolic extract obtained from the roots of P. farcta was identified as a rich source of phenolic and flavonoid compounds. The ethanolic extract showed effective antimicrobial and antioxidant properties. The results greatly indicated the promising effect of P. farcta root extract against Gram-positive and Gram-negative bacterial species. As the microbial resistance is constantly increasing, ethanolic extract of P. farcta root can be considered as a suitable complementary option to tackle this problem. In addition, the identification of individual components of P. farcta ethanolic extract and their biological functions or their combination with common antioxidant and antimicrobial agents could be the subject of future research.

Abstract Introduction: The Phaseolus Vulgaris on a global scale of cultivation has been categorized in the first rank and considered as the seventh staple food in the world. Proteins are usually classified into 3 groups including flours with 50 to 65 percent protein, concentrate with 65 percent protein and isolates with over 90 percent protein. Protein concentrate is produced by defatted flour through removal of soluble sugars, soluble fiber and minor compounds by alcohol, water or diluted acid with pH around 4-4.8. Protein isolate is affected by further refining processes compared to protein concentrate. In addition to the importance of the nutritional properties, crop proteins have a key role as a functional agent in the formulation of food products. Solubility, water binding capacity, oil absorption capacity, emulsifying and foaming abilities are noticed as the main functional properties. One of the specific and unique varieties of beans is Phaseolus Vulgaris var (aroos bean). The aim of this study was to determine the chemical composition of flour and bean protein isolate. In addition, some of the functional properties such as emulsification properties, foaming and stability as well as the effect of pH and ionic strength on above-mentioned characteristics were investigated. Materials and Methods: The seed of Phaseolus Vulgaris and refined soybean oil (0ila) were purchased from Sari local supermarket. Phaseolus Vulgaris protein isolate was extracted and obtained powder was kept in impervious polyethylene plastic bags against moisture and air and placed at -18 ° C until analysis. The efficiency was calculated based on the sediment. Also, chemical composition and purity of the isolates were determined. Some functional properties, including emulsifying properties and foaming capacity at pH 4, 7, 8 and 10 were tested; and impact of the ionic strength of NaCl in concentrations of 0, 0.5 and 1 molar were examined. The results of all treatments were expressed based on the average of triplicate. Means were subjected to analysis of variances (one way-ANOVA) using (SPSS Statistics version 16) software program. Differences among the mean values of the various treatments were determined by Duncan test and the significance was defined at p < 0.05. The graphs were drawn using Excel software. Results and Discussion: Phaseolus Vulgaris had 9% protein isolate efficiency and purity of 89.06%. The amount of protein in Phaseolus Vulgaris flour was less than the amount of protein that has been reported for other crops. While in terms of purity, protein isolate was similar to legumes in other studies. Foaming ability and emulsifying properties of Phaseolus Vulgaris protein isolate at 7 = pH were 42% and 65%, respectively. The value of this parameter using exposure to alkaline region and keeping far from the isoelectric point at pH = 10 were risen to the highest amount of 70.23% and 77.33%, respectively. During 90 minutes, stability of emulsions and foams were gradually decreased. With increasing of NaCl concentrations, foaming capacity and emulsifying properties were significantly reduced. Considering the efficient functional of Phaseolus Vulgaris protein isolate, further investigation required in order to be used in the formulation of meat products, cakes, cookies, crackers, sauces and soups.

Abstract Introduction: Fats are widely used in food formulations to improve nutrients and quality of food products. In recent years, consumer awareness of the relationship between diet and health has increased, which results in increasing concerns about fats in products, in terms of high levels of saturated fatty acids. Therefore, various attempts have been conducted to find appropriate method to produce solid fats with unsaturated fatty acid content. The production of low-fat cake is of special nutritional importance due to the effects of fats and the incidence of various diseases such as hypercholesterolemia and cardiovascular. In cake making, oil is very important, first of all, it has an effect on keeping the air in the cake dough. It causes porosity and increases the volume of the cake. The conversion of liquid oils to gel-like structures that have the properties of a solid fat (rheological properties, viscoelasticity, dispersibility, and softness) without using large amounts of saturated fats is an important development in the food industry, and structures produced by oleogelation are called oleogels. Therefore, the main purpose of this study was to produce low-calorie muffins by removing fat and replacing it with an oleogel system prepared from a soluble complex of xanthan gum and egg white by indirect foam molding.
 
Materials and Methods: First, aerogels were produced indirectly using egg white protein complex and xanthan gum, and then oleogel was produced by adsorption of oil. In this method, first, water suspensions containing 0.5 wt% of xanthan and 5 wt% of egg white protein were prepared. In the next step, the oleogel was used to produce muffin with reduced oil in three levels of 10, 30 and 50%. In this study, texture characteristics, color, porosity, water activity, moisture, specific volume of muffin samples were investigated. In the control sample, cake dough contained 100% wheat flour, 50% sugar powder, 30% liquid vegetable oil, 2% baking powder, 0.2% vanilla, 36% eggs and 12% invert syrup. To prepare samples containing oleogels, oil was removed and different concentrations of oil reduction at the level of 50, 30 and 10% in the formulation were added to each treatment. Results and Discussion: Muffin with 10% reduced oil had the highest volume and density and had similar texture characteristics to the control sample. By reducing the amount of fat to 50% of the initial amount in muffin, the volume decreased and the firmness of the texture increased significantly (p <0.05). The reduction of the percentage of porosity confirmed that the texture stiffness in higher values of substitution. The sensory evaluation showed that the 10% reduced oil sample had the highest consumer acceptance. According to the results of this study, it can be suggested that preparing muffins with oleogels can reduce the problems caused by fats, while improved sensory and qualitative properties and be produced as a functional food.

Abstract Antioxidant active packaging is one of the active packaging that prevents of lipid oxidation by releasing antioxidant during storage of food. In recent years the use of natural antioxidant instead of the synthetic variety in these packaging has been considered. The aim of this study was evaluating the performance of polymeric film coated with natural antioxidant (Rosemary Extract) to prevent lipid oxidation in butter. With three different concentrations (0.1%, 0.15%, 0.2%), the ethanolic extract of Rosemary was sprayed on the surface of low density polyethylene films (LDPE). Films without extract was used as control. Phenolic compounds migration from the surface of film to 95% ethanol (fatty food stimulant) was measured at 4ºc during 10 hours. With increasing time and extract concentration, migration to ethanol increased (P

Abstract Kurdish cheese is semi-firm cheese which has been traditionally manufactured from whole raw cow or sheep milk, in the East of Iran. It is ripened in goat’s skin bags. The microbial diversity of kordish cheese originates from natural microbiota present in the raw milk and its unique organoleptic properties have made it excellent among other traditional cheeses in Iran. This study has been conducted to evaluate microbiological diversity during ripening of Kurdish cheese. Samples were taken from 6 batches of cheese on day 1, 20, 40 and 60. Sensory properties of Kurdish cheeses were determined at 60,120 and 180 days of ripening. Based on results, the diversity and microorganisms count of were mainly influenced by ripening time. Lactic acid bacteria and Enterobacteriaceae were the predominant families of micro-organisms during the first 20 days of ripening and lactobacilli was the most abundant lactic acid bacteria in cheese. Coliforms and E.coli decreased more rapidly in cheese, whereas mows and yeasts decreased gradually and then increased in ending days. At the end of ripening period, mo coliforms, salmonella and coagulase positive staphylococcus were detected in the samples. Changes in textural and chemical characteristics in relation to ripening time showed that; pH, Moisture, Total solid, aw and fat content of samples decreased during 40d and reached to 4.69, 33.9, 64.07, 0.927, 28.1 at 60 days respectively. no significant variations observed in Protein values during ripening period. The values for sensory characteristics such as taste, aroma, colour and total acceptance of the full-ripened cheeses (120th days) increased as ripening progressed. According to our results, from a safety point of view, the optimum time for consuming Kurish cheese would be after passing ripening time of 60 days (during 1-2 months) due to decrease in pH, undesirable flora and increase in desirable LAB counts.

Abstract Introduction
Addition of dietary fiber sources to high-consumption food products is a smart way to increase the amount of fiber intake through the daily diet. Biscuits are one of the most popular bakery products. Wheat flour is the most significant ingredient for biscuit production in terms of quantity, its quality plays a major role in the quality of the final product, especially in terms of nutritional value and texture. Fiber sources such as cereals, legumes, and other plant products such as nuts, fruits, and vegetables can be used to enrich the wheat flour with the aim of improving the nutritional properties of wheat flour-based products.
Lentils are one of the most important legumes with high nutritional value and low anti-nutritional factors, traditionally consumed as a minimally processed product. The functional properties of lentil proteins are one of the reasons for their special potential application in the production of various types of food products. Due to the presence of various essential amino acids such as leucine, isoleucine, lysine, phenylalanine, and valine, as well as high levels of fiber, B group of vitamins, and minerals, the addition of lentil flour, even flour prepared from low-grade lentils, aids in improving wheat flour and increasing the quality of bakery products.
In recent years, attention of many manufacturers and researchers has been directed towards the use of various cereal bran as a source of fiber in the production of bakery products. Rice bran is a by-product of rice processing and milling and, as a cheap source of fiber, can be included in the human diet. Considering the importance and benefits of using dietary fibers in bakery products, in the present study, the effects of using roasted lentil flour and rice bran powder (at 0, 3, 6, 9, 12 and 15% l) as natural sources of dietary fiber, on the physicochemical and sensory properties of biscuits were studied.
Materials and Methods
To prepare the biscuit dough, 350 gr flour, 105 gr f sugar, 105 gr shortening, one egg, 3.5 gr vanilla, 1.75 gr salt, and 10.5 gr baking powder were used. After molding, baking was done at 160 °C for 15 minutes.
The moisture of biscuit was measured using AACC 44-19 method and pH was measured using a digital pH meter (3020, Jenway, UK). Ash was measured according to AACC 08-01 standard method (2000), protein was measured according to AACC 46-18 standard method (1999), and the raw fiber was measured after acid and alkaline digestion according to AACC 32-10 standard method (2000). The Folin-Ciocalteu method was used to measure the total phenolic content, and free radical scavenging capacity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Dimensions of biscuit (diameter and thickness) were measured using calipers, biscuit texture firmness was measured using a Brookfield texture analyzer (Brookfield, CT3, USA), and biscuit color (a* (red-green), b* (yellow-blue), and L* (lightness or whiteness) indices were measured using a colorimeter (TES-135A, Taiwan). Evaluation of sensory characteristics including color, taste, crispness and firmness of texture, and overall acceptance was performed using the five-point hedonic scale method. Data were analyzed by one-way ANOVA (SPSS 26, Duncan, P<0.05).
Results and Discussion
The results of statistical analysis showed that partial replacement of wheat flour in the biscuit formula with roasted lentil flour and rice bran powder increased the contents of fiber (to 1.39%), protein (to 8.62%), ash (to 1.97%), total phenols (to 131.27 mg GAE/kg), diameter (to 4.4 cm), expansion coefficient (to 8.23), yellowness (to 26.79), redness (to 6.92) and browning index (to 58.07) of the biscuit. Following the increase in the level of total phenols, the antioxidant activity (to 31.9%) of the biscuit also increased. Although using roasted lentil flour decreased fat content of the biscuit but rice bran powder retrieve it (to 23.65%). By reducing the amount of wheat flour and increasing the amount of roasted lentil flour and rice bran powder, the pH (to 6.62), moisture (to 2.95%), thickness (to 0.54 cm), texture firmness (to 1686 gf), and lightness (to 66.13) of the biscuit reduced. The scores of sensory attributes decreased with increasing the level of replacement of wheat flour with studied additives. However, the results indicated the overall acceptability of biscuits containing low levels (3-9%) of roasted lentil flour and rice bran powder.
Conclusion
Given that roasted lentil flour and rice bran powder are rich in fiber and contain appropriate amounts of vitamins and minerals, and considering the growing consumer demand for such nutrients,  replacing these items with wheat flour (at 3-9%) in production of high-consumption products such as biscuits will contribute significantly to health-related issues, especially in terms of obtaining the recommended amounts of fiber.

Abstract Introduction: Spirulina platensis belongs to the Division of Cyanobacteria and the family of Oscillatoriaceae. It is autotroph and photo-synthesizer and can be reproduced through double cell division. Spirulina platensis is a filamentous blue-green multi-cellular microalgae naturally occurring in the tropical and alkaline lakes of America, Mexico, Asia and central Africa. It contains unique and extraordinary nutrients which can be used in the production of functional foods. Among bakery and flour products, cake has a relatively high diversity and long shelf-life and is famous among a variety of people, especially children. Since the knowledge associated with the enrichment of sponge cake, as a popular product among different communities (in particular, children) is limited, the aim of the present research is to produce a sponge cake enriched with spirulina platensis, and to examine its nutritional, physicochemical and sensory properties.
 
Materials and methods: In this research, the effect of Spirulina platensis at four levels (0, 0.5, 1 and 1.5%) was investigated on the nutritional properties (protein, fat, iron, zinc and copper contents), physicochemical properties (moisture content, pH, total phenol content, antioxidant potential, textural properties and color indices) and sensory attributes (odor, color, texture, flavor and taste, chewiness and total acceptance) of the sponge cake samples prepared with wheat flour.
 
Results and discussion: Results showed that algae powder was rich in protein (56.33%) and iron (13.18 ppm). The addition of Spirulina platensis to the sponge cake caused reduction in its moisture content during storage (days 1, 5 and 10). The results also revealed that the different levels of algae addition brought about significant differences in the moisture content of the samples (p<0.05). The results also indicated that the protein, fat and mineral contents as well as other nutritional properties of the sponge cake increased as the algae content was elevated. The total phenol content of the cake samples was also raised with an increase in the algae powder level, compared with the control. This could be attributed to the large amounts of phytochemical and biological active substances such as flavonoids, sterols and other phenolic compounds. The results demonstrated that the porosity values of the control and the sample containing 1.5% of the algae were not significantly (p<0.05) different. The percentage of porosity was equal to 24.94, 37.99, 33.39 and 27.81 in the control and the samples containing 0.5, 1 and 1.5% of Spirulina platensis. Overall, the sample with 0.5% of the algae and the control had the highest and lowest porosity percentage respectively. As the algae level rose, the textural parameters (hardness, cohesiveness and gumminess) of the sponge cake increased on days 1, 5 and 10 of the storage period. The colorimetry results showed that the effect of Spirulina platensis was significant (p<0.05) on color parameters (L*, a* and b*). Sensory evaluation revealed that the sponge cake with 0.5% of the microalgae was the most acceptable among the samples. The green color of the cake crumb was attractive to the panelists and a comparison between the total sensory scores indicated that the incorporation of Spirulina platensis into the sponge cake was desirable from the panelists` points of view. The results of this study demonstrated that the addition of Spirulina platensis to sponge cake for the enrichment of this product, caused an increase in its protein and mineral contents, antioxidant potential, in addition to other functional ingredients naturally occurring in this algae. The results revealed that different levels of this algae (0.5, 1, and 1.5%) did not significantly affect the cake texture, however, had a significant impact on its color. Incorporation of Spirulina platensis into sponge cake reduced its L*, a* and b*, which was highly noticed by the panelists. Therefore, it can be declared that enrichment of sponge cake, as a popular product among different people of societies, particularly children, is a desirable and easy way of transferring the useful and valuable compounds of this algae to human.

Abstract Introduction
Today, many edible oils such as palms, corn, soybeans and sunflowers are used in food preparation. Essential oleic, linoleic and linolenic fatty acids, found abundantly in olive, sunflower and soybean oils, respectively, play an important role in maintaining health. Antioxidant compounds are used to increase the shelf life of oils, which are classified into two groups of natural and synthetic antioxidants based on the source of production. Phenolic acids are a subset of a large group of phenolic compounds that are used as natural antioxidants in industry. Gallic acid is much stronger than protocatchuic acid due to its three hydroxyl groups. However, the presence of more than three hydroxyl groups does not seem to increase the antioxidant effect in oily systems. The position of the hydroxyl group on the aromatic ring also affects the antioxidant activity, so that the replacement of the hydroxyl group in the ortho and para position increases the antioxidant activity of phenolic acids. Methyl gallate, which is widely found in plants and polyphenolic secondary metabolites, is a natural antioxidant. Despite efforts to date, no suitable natural antioxidant has been proposed to control the thermal oxidation of oils at high temperatures. Therefore, due to the widespread use of oils in food, the thermal stability of natural antioxidants gallic acid and methyl-gallate compared to the powerful but synthetic antioxidant TBHQ, depending on the degree of satiety of the lipid system (sunflower oil and olive oil) and 80 degrees Celsius will be evaluated in this study.
 
Material and Methods
Samples of sunflower and olive oil were purchased from local stores. All chemicals and solvents were provided by Merck and Charlot. Sunflower and olive oil were purified by column chromatography to remove natural antioxidants. Oxidation of purified sunflower oil (1 g per oil) in the presence of a concentration level of gallic acid, methyl gallate and TBHQ (1.2 μmol/g) in glass bottles. The rate of progression of oxidative reactions and the evaluation of oil quality during temperature application is possible by measuring the peroxide number. The carbonyl number is determined using 2-propanol as solvent and 2,4 decodenal as standard and absorbance at 420 nm. The effect of antioxidants (InH) on the oxidation of the test samples can be measured based on the kinetic parameters. These parameters are stability factor F, ORR oxidation rate ratio, activity A and average consumption of WInH antioxidants.
 
Results and Discussion
The minimum and maximum induction times are related to the control sample and the sample containing the synthetic antioxidant TBHQ, respectively, which, considering the position of the two hydroxyl groups in the para position relative to each other in the TBHQ molecule, make this antioxidant stronger. Justifies. At 80 °C and in sunflower oil, the antioxidant methyl gallate shows a more effective stability factor (F) and antioxidant activity (A), indicating greater antioxidant power than gallic acid. Similarly, F-ORR-A values in methylgalate treatment have a significant effectiveness compared to other treatments. The higher oxidative stability of olive oil against sunflower oils can be attributed to the small amounts of oleic acid and especially the small amounts of linolenic acid in olives. Stability factor (F), is significantly higher for the TBHQ antioxidant than the values obtained for the other two. This factor is affected by the induction period of antioxidants and can be expected due to the effectiveness of antioxidants in increasing the duration of the induction period. The highest value obtained for the ORR oxidation rate parameter, is related to the antioxidant gallic acid. The parameter of antioxidant activity A, in TBHQ is higher than the other two antioxidants. Measurement of carbonyl compounds resulting from the decomposition of hydroperoxides is a good measure of the rate of development of oxidative reactions. In the treatment of gallic acid and TBHQ, the changes in the carbonyl number decrease at the end of the annealing, which is probably due to the decomposition of carbonyl compounds and the production of polymer compounds. Which cannot be measured by carbonyl number test.
 
Conclusion
Better efficacy of gallic acid compared to methyl gallate in olive oil and better efficacy of methyl gallate compared to gallic acid in sunflower oil at 80 °C show the composition of fatty acids, the nature of lipid systems and the position of antioxidants in the reaction medium. Have a great effect on improving the performance of antioxidants. Determination of oxidative stability based on carbonyl number shows similar results to peroxide number .

Abstract Background and Objectives
 Natural preservatives extracted from herbs are important sources for bioactive compounds that can be used in protection of food products. Essential oils are aromatic oily liquids, obtained from plant material like flowers, buds, seeds, leaves, and roots. Unfortunately, most natural compounds are biologically instable, poorly soluble in water and they distribute poorly to target sites. Currently, some novel methods have been introduced in order to improve their stability and their bioavailability, among which is the use of liposomal encapsulation. Microencapsulation reduces reactivity with the environment (water, oxygen, light), decreases the evaporation or the transfer rate to the outside environment, promotes handling ability, masks taste and enhances dilution to achieve a uniform distribution in the final product when used in very small amounts. Essential oils, as natural extracted compounds extracted from plants, are unstable compounds with low water solubility and unable to achieve target cells. Essential oils encapsulation by nanoliposomes is a novel method for increasing their biological activity and protecting them from destructive factors. The aim of this study was production and optimization of nanoliposomes containing Z. teniur essential oil and investigating their antibacterial effects against pathogens (Staphylococcus aureus and Escherichia coli).
 
Materials and Methods
 Lipid film hydration method was used to produce nanoliposomes containing Z. teniur essential oil. Soy phosphatidylcholine and cholesterol were the main wall materials and chloroform was used as the mixing solvent . The particle size of nanoliposomes and their zeta-potential were investigated using laser diffraction method. In order to determine the minimum inhibitory concentration and the minimum bactericidal concentration of Z. teniur essential oil against examined bacteria, serial dilution method was used. Also, antioxidant activity of free and nano-encapsulated essential oil of Z. teniur was determined by DPPH method.
 
Results
 According to the results, highest encapsulation efficiency achieved by using 80:20 ratio of soy phosphatidylcholine to cholesterol in nanoliposomes’ wall structures. In general, by increasing the ratio of phosphatidylcholine to cholesterol, encapsulation efficiency was improved. Zeta-potential of nanoliposomes was equal to -5.3 mv and mean particle sizes were in the range of 94.7-119.9 nm. Results indicated that essential oil ejection from nanoliposomes has direct relation to the time of storage and after 30 hours, ejection rate will increase considerably. Ejection rate was higher in phosphate buffer pH=7.4 in comparison with phosphate buffer pH=5.4. Minimum inhibitory concentration and minimum bactericidal concentration of free essential oil against Escherichia coli was 100 and 175 (µl/ml) respectively. Although, Minimum inhibitory concentration and minimum bactericidal concentration of nanoliposomes containing Z. teniur essential oil were equal to 75 and 150 (µl/ml) respectively. Also, results shown that , minimum inhibitory concentration and minimum bactericidal concentration of encapsulated Z. teniur essential oil against Staphylococcus aureus were lower in comparison with free form of Z. teniur essential oil. Staphylococcus aureus (as Gram-positive bacteria) was more susceptible than Escherichia coli (as Gram-negative bacteria).
 
Conclusion
 Encapsulation of Z. teniur essential oil by nanoliposomes led to improve antibacterial effects of essential oil against Staphylococcus aureus and Escherichia coli. Also, investigating of antioxidant activity showed that encapsulated Z. teniur essential oil in nanoliposomes was more effective than free form of Z. teniur essential oil in scavenging of DPPH free radicals. Using nanoliposome encapsulation technology can be an effective way for increasing the efficiency of natural antibacterial compounds and essential oils encapsulated in nanoliposomes are suitable alternatives for synthetic preservatives used in food industry nowadays. The use of liposomes containing Z. teniur essential oil can provide the necessary protection against growth of spoilage and pathogenic microorganisms such as Staphylococcus aureus and Escherichia coli in food products.
 

Abstract Introduction: Bee pollen, commonly referred as the ‘‘life-giving dust’’, results from the agglutination of flower pollens with nectar using salivary substances of the honeybees (Almeida-Muradian et al., 2005). Pollen contains 10 to 40% protein, 1 to 13% lipid, 13 to 55% carbohydrates and 2 to 6% minerals. Royal Jelly is produced by enzymatic digesting of bee pollen by proteases and other natural enzymes. Based on dry weight, it contains 27-41% protein, 30% carbohydrates, 8-19% lipids, minerals, trace elements and some vitamins (Sabatini et al., 2009; Wytrychowski et al., 2013). The antioxidant properties of royal jelly and bee pollen, are related to main proteins and phenolic compounds and flavonoids (Nagai and Inue, 2004). The antioxidant activity of peptides can be evaluated using DPPH, radicals scavenging activity, Ferric reducing, Ferrous chelating activity (Khantaphant et al., 2011). Antioxidant and ACE inhibitory activity of pollen, royal jelly and peptides were investigated by different researchers (Bogdanov, 2014; Morais et al., 2011; Salampessy et al., 2015; Marinova and Tchorbanov, 2010; Wiriyaphan et al., 2012). The objective of present research was optimization of enzymatic hydrolysis of bee pollen protein by Alcalase according to its antioxidant and ACE inhibitory activity compared to royal jelly. Materials and methods: The preparation of the bee pollen extract was performed by mixing the bee pollen with water (1:10) (w/v). The macerates were filtered and centrifuged at 12000 g. The obtained supernatant was lyophilized. The royal jelly extract were prepared using method described by Liu et al., 2008. The total phenolic content of the extracts was recorded using the Folin–Ciocalteu method (Moreira et al., 2008). DPPH radical-scavenging activity was determined as described by Bersuder, Hole, and Smith (1998). The ability of the hydrolysate to reduce iron (III) was determined according to the method of Bougatef et al. (2008). Bee pollen was added and homogenized with 5 volumes of distilled water. pH and temperature of the solution were adjusted to pH=8 and 50◦C. Alcalase in the concentration range of 1 to 2% w/w were added to the pollen protein solution. Enzymatic hydrolysis performed during different times 2 to 5 hours. Hydrolysis was stopped by heating at 80˚C for 10 min. The hydrolysats were centrifuged at 4000x g for 30 min to remove the residue. The supernatants were pooled and then lyophilized (Matsuoka et al., 2012). DPPH radical scavenging ability and reducing power of pollen hydrolysates of pollen hydrolysates were measured. Also ACE-inhibitory activity of pollen hydrolysates was measured was assayed by method reported by Nakamura et al. (1995). Statistical analysis of results before hydrolysis was done by SPSS. Optimization of enzymatic hydrolysis was done by Response Surface Methodology (RSM) in Design Expert software. Results and discussion: Total phenol value measured for pollen ranged between 48.15 to 174 mg Gallic acid/g for royal jelly ranged from 9.24 to 87.261 mg Gallic acid/g. Considering that royal jelly is obtained by direct digestion of pollen, the amounts of their phenolic compounds were comparable (Bogdanov, 2014). Phenolic compounds increased by increasing concentration royal jelly and pollen extract in dose dependent manner. Increasing concentrations of royal jelly in range of 300 to 1000 mg/l was more effective than pollen (p

Abstract Introduction: Dairy products fortification especially ice cream is one of the most important goals and priorities of today's humanbeing to produce health food products. Protein isolate is one of the compounds used to produce health products.The aim of this study was to produce ice cream with new features based on a functional material by partially replacing dry matter with wheat germ protein isolate.
 
Materials and Methods: In present research wheat germ protein isolate with 1%, 3% and 5% was extracted and added to ice cream. In the first step, protein isolate was evaluated by tests of moisture content, fat percentage, acid insoluble ash, particle size and flour grain size. In the next step, protein isolate of wheat germ by 1, 3 and 5 percent was added to ice cream mix and its effects on rheological and texture properties, color, physico-chemical and sensory parameters of ice cream were evaluated.
 
Results and discussion: The results showed that the moisture, fat and acid insoluble ash of protein isolate were 8.41±0.42, 11.34±0.44 and 3.57±0.23 respectively. The particle size and flour grain size was 282±0.01. Laboratory data showed that by increasing protein isolate replacement, the viscosity, melting resistance and volumizing index were reduced significantly. Whereas, texture, hardness and adhesion parameters, density, particle size and diameter, specific distribution and surface area, and yellowness and redness indices were significantly increased. All the laboratory results and sensory evaluation of the samples revealed that the ice cream sample with 1 percent of wheat germ protein isolate was a preferred and optimized sample.

Abstract Introduction: Metal oxide nanoparticles have unique physical and chemical properties. These components have shown antimicrobial effects against a wide range of microorganisms. In order to improve the physical properties of metal oxide nanoparticles, doping other elements with metal oxide nanoparticles is an effective way. Bacillus cereus is a gram-positive bacteria causing food-borne diseases. In this study, the antimicrobial effects of doped zinc oxide nanoparticles with manganese or iron on Bacillus cereus have been studied. To investigate the synergistic effects of the combined nanoparticles with two common biocides, including hydrogen peroxide and sodium hypochlorite, have been used.  Materials and methods: Co- precipitation method was used to prepare nanoparticles of manganese-zinc oxide and iron-zinc oxide. In this method, zinc sulfate and manganese sulfate were used to prepare manganese-zinc oxide  and iron sulfate and zinc sulfate are used for Zn- Fe doped nanoparticles. After preparing the sulfate solutions, the sulfate solutions were mixed and placed in an ultrasonic apparatus at a frequency of 57 kHz for 2 hours at 50ºC. Then, it was stirred at 80°C. A solution of NaOH was added until the pH of the solution reached 12. In these conditions, the mixing was done for 30 minutes. The solution was placed at ambient temperature for 18 hours. Then the centrifuge was performed to separate the sediment. Purification was done through washing with distilled water and ethanol. The precipitates were dried in the vacuum oven. In this way, the doped nanoparticles of manganese-zinc oxide and iron-zinc oxide were obtained. The Fourier transform infrared spectrum (FTIR) was carried out by the Perkin-Elmer apparatus of the Spectroma2 model, using a dry potassium bromide tablet at a frequency range of 4500-4000 cm-1. The X-ray diffraction was tested using the Phillips PW1820 from 2º to 80º. Structure of produced nanoparticles was assessed by the HITACHI electron microscope, the H-7500 model, by placing a drop of nanoparticles dissolved in methanol on a special lining with carbon coating and air drying, and performing microscopic images using an electron microscope in 100kv. The bacteria used in this study included Bacillus cereus (PTCC 1665) was purchased from the Iranian Scientific and Industrial Research Center and was transferred to the BHI medium in sterile condition and incubated for 32 hours at a temperature of 32°C. Microbial cells were isolated by centrifugation at 4000 rpm. McFarland's method was used for determining the bacterial population. Dilution was carried out to reach a population of about 106 CFU/ml. Agar disc diffusion method was used for assessing the antimicrobial effect of the doped nanoparticles alone or in combination with tested biocides (hydrogen peroxide, sodium hypochlorite). At first, 106 CFU/ml of Bacillus cereus were inoculated on the surface of Blood Agar. Then, 5, 10, 20, 30, 50, 100 and 200 mg/L of each of the nanoparticles were placed on the surface of the culture medium and then the plates was incubated at 37°C for 24 hours. Inhibition zone was considered as antibacterial activity. In order to investigate synergistic effects, inhibitory fraction index test was calculated. All experiments were performed in three replications. Statistical analyzes were performed using STATISTICA software.  Results and discussion: Results obtained from X-ray and FTIR analysis of doped nanoparticles confirmed that co- precipitation is a suitable method for producing doped nanoparticles of zinc oxide. TEM analysis of produced nanoparticles also affirm formation of doped nanoparticles of zinc oxide with manganese and iron. The results of antimicrobial tests showed that Mn-Zn oxide nanoparticles have more antimicrobial effects on Bacillus cereus than zinc oxide (32mm inhibition zone) whereas Fe- Zn oxide nanoparticles cause inhibition zone about 12 mm. In addition, both doped nanoparticles have more antimicrobial effects than zinc oxide nanoparticles alone, resulted in doping process improves antimicrobial properties of zinc oxide. The synergistic effects of synthetic nanoparticles in the combination of two common antimicrobial agents, including hydrogen peroxide and sodium hypochlorite, have been identified. Both nanoparticles show synergistic effects in combination with two tested biocides (especially in high concentrations). A mixture of two biocides with nanoparticles increases their antimicrobial properties. Manganese-zinc oxide nanoparticles with hydrogen peroxide and sodium hypochlorite showed a partial synergistic effect at low concentrations (5 + 20) and a complete synergistic effect at higher concentrations. In the case of iron-zinc oxide, combination of this nanoparticle with hydrogen peroxide and sodium hypochlorite, has complete synergistic effects at high concentration (100 + 200) and at other conditions, shows partial synergistic effects.

Abstract Introduction: The quality of breads depends on the baking capability of flour, fermentation time, protein content, and type of additives. An economically important concern of the baking industry is delayingthe bread staling. In addition, the staling process changes outer and inner properties, scent, flavor, and chewiness of breads, which in turn leads to staled or, in other words, non-fresh breads. The application of lactic sourdough and/or other techniques such as improved dough making, improved baking quality, packaging, and use of additives and improvers can be highly effective in delaying this process. One of the additives that improve the properties of baked products is the α-amylase enzyme. α- amylase can improve the elastic properties of the texture while delaying staling. This enzyme can also form useful sugars for yeasts and increase gas formation in dough, which in turn improves and increases the bread volume. Against this background, the current study analyzed the effects of sourdough and α-amylase on qualitative characteristics, physicochemical properties, staleness, size and sensory profile of the toast breads. Materials and methods: The ingredients of the toast bread dough, i.e. 78% wheat flour (1 kg), sugare (1-2 w/w%), salt (1.8-2.2%), oil (3-6%), yeast (1.5-2%), α-amylase (0.01-0.03%), lactic sourdough (4 and 6% of wheat weight), were prepared and weighed to make the dough required for the baking process. The dough samples were spread and then rolled after an initial resting of 10-15 minutes. The rolls (approx. 580 g) were transferred to a cast. The dough samples were finally moved to the fermentation chamber at 35°C and 80% relative humidity for final fermentation and baking. Their analyzed using different physical, chemical, and sensory tests. A completely randomized design with three replications was used to analyze experimental data (except for staleness data of bread samples from sensory and instrumental tests which were analyzed using factorial experiment with a completely randomized design). Means were compared by Duncan's multiple-range test (α = 1%) in SPSS 16. Results and discussion: According to the results, α-amylase and lactic sourdough caused an increase in bread’s MC compared to the control samples. In other words, starch hydrolysis by α-amylase created free water in the dough. Moreover, sourdough increased water absorption of wheat through mechanisms including production of exopolysaccharides and increased production of pentosans. The application of α-amylase and lactic sourdough also increased the ash content of the breads compared to the control samples because they increased the production of dextrins and synthesis of exopolysaccharides. It should be mentioned that these additives decreased the pH of breads compared to the control samples. This is because the sourdough bacteria produced lactic, acetic and other acids that acidified the medium and reduced the pH. This enzyme also produced metabolites like dextrins and increased the amount of fermented active ingredients, which further reduced the pH. The proteolytic activity of lactic bacteria of the acid in sourdough led to decomposition of proteins, particularly gluten, into amino acids. This also reduced the protein content and diluted the gluten in breads. The results showed that the lactic acid bacteria of the sourdough hydrolyzed the fat control of bread samples by their lipase and thus lowered the fat content in bread samples compared to the control. The fiber content of samples was higher than that in the control as a result of enzyme and sourdough application. This is due to the fact that fermentation increases the pentosan content and reduces their molecular size. It also increased solubility of insoluble fibers like beta-glucans. On the other hand, their application led to larger bread size than the control because α-amylase hydrolyzes the starch and breaks the amylose and amylopectin chains to convert them into disaccharide substrates. Accordingly, yeasts could grow better and produce more CO2 in the dough, increasing the bread size. The application of this enzyme and lactic sourdough gave a brighter appearance to toast bread samples whereas a* and b* were reduced. This is because the bread undergoes changes in its crust color during the baking process, which are mainly caused by maillard and caramelization reactions. It is worth noting that these additives reduced the staleness of the samples. With respect to staling, the gluten content and its ratio to starch are highly effective. During the bread shelf life, as its kinetic energy decreases, the cross-linking increases and intensities. In other words, the bread becomes harder and staler. However, using α-amylase and sourdough, the bread hardness and staleness are decreased as the starch swelling is limited and cross-linking between protein and gluten is inhibited. These two additives improved most sensory properties including resistance to breakage and rupture, porosity, kernel color, crumb color, proportionality of shape, back-side uniformity, flavor, taste, texture, chewiness, and larger size of the bread samples. This reason for these improvements can be due to starch hydrolysis by α-amylase and production of dextrins and CO2. According to the results, the bread sample containing 0.03% α-amylase and 6% lactic sourdough was selected as the best sample.

Abstract An accelerated shelf-life test using elevated temperatures 62, 72 and 82 ˚C was conducted to predict the oxidation progression of walnuts over a long-term storage. Oxidation parameters including values of conjugated dienes (CD) and trienes (CT) values and thiobarbituric acid (TBA) value were employed to evaluate the oxidation processes. Changes followed an apparent first-order kinetic. Walnuts and walnut kernels were also kept in normal condition (20-30˚С; RH, 35-45%) for 12 months in order to validate the approach. A maximum of energy (62.24-75.67 kJ mol-1 K-1) needed for formation of primary oxidation products and a minimum of energy (35.65 kJ mol-1 K-1) to generate secondary oxidation products were calculated. Formation of oxidation products in walnut kernels was found to be a temperature-dependent reaction, with Q10 =1.44- 2.1. The results showed that CD and CT values could provide a proper estimation for oxidative stability of the nuts stored in ordinary condition, with an average error of approximately 12.9%.