Vida Mardani Ghahfarokhi; Mehran Alami; Saeedeh Arabshahi- Delouee; Alireza Sadeghi Mahoonak
Abstract
In this study, phenolic compounds of evening primrose leaves (Oenothera biennis) were extracted with aceton 70%, ethanol 70% and methanol 70%. Total phenolic content of aceton extract was significantly higher than the ethanolic and methanolic extracts. Extracts were tested for their antimicrobial activity ...
Read More
In this study, phenolic compounds of evening primrose leaves (Oenothera biennis) were extracted with aceton 70%, ethanol 70% and methanol 70%. Total phenolic content of aceton extract was significantly higher than the ethanolic and methanolic extracts. Extracts were tested for their antimicrobial activity against Lactobacillis plantarum and Saccharomyces cerevisiae, using micro broth dilution method. All extracts showed good antimicrobial activity against tested microorganisms. Aceton extract had the most efficient antimicrobial activity. Lactobacillis plantarum was found to be more sensitive to aceton extract than Saccharomyces cerevisiae. The MBC values of aceton extract for Lactobacillis plantarum and Saccharomyces cerevisiae was 0.5 and 1 (μg/ml), respectively. The antimicrobial activity of aceton extract (0.25, 0.5 and 1 μg/ml) was also evaluated in apple juice inoculated using Lactobacillis plantarum and Saccharomyces cerevisiae during 35 days storage at ambinent temprature. Concerning microbial data in apple juice, the extract was able to control Lactobacillis plantarum and Saccharomyces cerevisiae growth. Aceton extract also showed higher inactivation effect on Lactobacillis plantarum in apple juice. Phenolic compounds did not change during 35 days of storage. No change in organoleptic quality of apple juice treated with aceton extract (0.25 μg/ml) was observed, according to sensory evaluation.
Vida Mardani Ghahfarokhi; Mehran Alami; Saeedeh Arabshahi- Delouee; Rasul Khoda Bakhshi; Maryam Ghaderi
Abstract
Evening primrose (Oenothera biennis) is a biennial flowering plant increasingly cultivated for medicinal use of γ-linolenic acid rich oil of its seeds. In this study, total phenolic and flavonoids content, antioxidant and antimicrobial activity of aceton, ethanolic and metanolic extracts of evening ...
Read More
Evening primrose (Oenothera biennis) is a biennial flowering plant increasingly cultivated for medicinal use of γ-linolenic acid rich oil of its seeds. In this study, total phenolic and flavonoids content, antioxidant and antimicrobial activity of aceton, ethanolic and metanolic extracts of evening primrose flowers were evaluated. Aceton extract had the highest extraction yield, total phenol and flavonoids content. Extracts were also tested for their antioxidant activity using scavenging activity of 2,2- diphenyl-1-picrylhydrazyl and reducing power of Fe+3 in comparision with BHT. BHT was the highest in DPPH radical scavenging and reducing power followed by aceton, ethanolic and methanolic extracts. The extracts also showed good antibacterial activity against all of tested microorganism and the tested gram-negative bacteria was more resistant to inhibitory effect of extracts. Among different bacteria tested, Salmonella. typhimurium was found to be the most resistant to aceton and ethanolic extracts. The MBC values, with respect to aceton and ethanolic extracts for Salmonella. typhimurium were 10 and 20 (μg/ml), respectively
