Food Biotechnology
Hadis Taghvatalab; Dornoush Jafarpour
Abstract
Introduction Scientific evidence is mounting that synthetic chemicals used as food additives may have harmful impacts on health and the biological system and cause many diseases and damages to the human body. Also, many consumers are concerned about the use of artificial ingredients to maintain ...
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Introduction Scientific evidence is mounting that synthetic chemicals used as food additives may have harmful impacts on health and the biological system and cause many diseases and damages to the human body. Also, many consumers are concerned about the use of artificial ingredients to maintain the quality and safety of foods. Therefore, the use of natural preservatives and food preservation methods based on natural compounds have attracted the attention of researchers. Edible films and coatings are useful materials, mainly produced from biodegradable polymers including polysaccharides (gums), proteins, and lipids, and are commonly used for the shelf life extension of foods. The primary edible films /coatings are promising alternative methods to preserve, and retard the adverse chemical reactions and microbial growth. They also can act as a carrier of antimicrobials, antioxidant substances, and other additives. Sage seed gum (SSG) is a water-soluble polysaccharide obtained from Sage (Salvia macrosiphon). It is an environmentally-friendly biodegradable material that can form high-viscosity aqueous solution and exhibit pseudoplastic behavior. Essential oils (EOs) are volatile and aromatic oily liquids extracted from various plants. Most of the EOs have antimicrobial and antioxidant activities due to their phenolic compounds, terpenes and terpenoids. A promising technique is incorporating EOs into coating solutions as active film/coating to extend the shelf life of food products. Bay leaf (Laurus nobilis) is an aromatic evergreen tree or large shrub with green, glabrous leaves. It is used as a flavoring agent and an essential ingredient in food preparation. Bay leaf has received much attention due to its antimicrobial, antioxidant, anti-inflammatory and immune system stimulating properties. Hence, the aim of the present study was to evaluate the antimicrobial and antioxidant properties of SSG coating incorporated with different concentrations of bay leaf EO (BLEO) and its nanoemulsion (BLNEO). Materials and Methods The active packaging was produced based on the gum of sage seed containing BLEO and BLNEO. After preparing the EO from bay leaves, their corresponding NEO was produced and the characterization of nanoparticles was evaluated in terms of droplet size, polydispersity index (PDI) and zeta potentials. Then, the antimicrobial and antioxidant properties of BLEO and BLNEO were compared. After that, SSG coatings were prepared with 1.5% and 3% BLEO and their corresponding NEO forms. Subsequently, the antioxidant (DPPH and ABTS) and antimicrobial (against Bacillus cereus, and Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli) properties of the produced films were investigated. Results and DiscussionGas chromatography-mass spectrometry (GC-MS) identified 1,8-Cineole and α- Terpinyl acetate as the major components of BLEO. The BLNEO exhibited a droplet size of approximately 92.4 nm and a zeta potential of -45.1 mV. In comparison to the control and SSG, it was found that the group comprising EO and NEO significantly (p<0.05) showed superior free radical scavenging capacity. SSG-3% BLNEO had the highest DPPH inhibition percentage (69.54%). According to the results, EO at the nanoscale can scavenge more free radicals than EO (p<0.05). Antimicrobial inhibition zone of different treatments against selected gram positive and gram negative bacteria showed that all bacteria were strongly inhibited after the addition of BLEO into the SSG. Moreover, data revealed that the growth of the studied pathogens was completely inhibited in a dose-dependent manner (p<0.05). SSG-BLNEO exhibited better antimicrobial activity than SSG-BLEO coating and its antimicrobial activity was significantly enhanced by increasing BLNEO concentration (p<0.05). This phenomenon is attributed to the protective role of encapsulation and the slow release of EO from the coating matrix, resulting in enhanced antimicrobial activity. Nanoemulsions, owing to their small droplet size and high surface area, offer superior efficacy compared to conventional emulsions. Consequently, the gradual release of essential oils from nanoemulsion-based edible coatings contributes to their enhanced antimicrobial performance. ConclusionThese findings suggest that the SSG-BLNEO edible active coating possesses promising applications as an antimicrobial and antioxidant agent for food packaging applications.
Food Technology
Dornoush Jafarpour; Parisa Ataei
Abstract
Introduction: Every year during marine fishing, some species that are caught are not suitable for human consumption and they are known as surplus fishing or low consumption, which is an important factor in wasting these rich resources. Therefore, it is necessary to set arrangements to use such species ...
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Introduction: Every year during marine fishing, some species that are caught are not suitable for human consumption and they are known as surplus fishing or low consumption, which is an important factor in wasting these rich resources. Therefore, it is necessary to set arrangements to use such species for human consumption, including the production of surimi. The surimi industry mainly uses Alaska pollock fish as the main source for surimi production. However, due to the increase in the world’s population and partly the depletion of the fish stocks, followed by a reduction in the surimi production of Allaska Pollock fish (due to restrictive fishing laws), the need to use new species is considered urgent. In this regard, additives such as gums can be used to reform and improve the properties of surimi. Therefore, in this study, the possibility of producing surimi paste and gel from Lizardfish and Talang Queenfish was investigated and the effect of Konjac on the physicochemical properties of the product produced from these two types of fish was evaluated. Materials and methods: In this study, Talang Queenfish and Lizardfish with approximate weight of 225.9±33.6 and 275.9±24.4 g and average size of 15.1±2.4 and 17.3± 2.6 cm, respectively, were purchased freshly from the fish market. After preparing surimi paste from both types of fish, Konjac gum in concentrations of 0.25, 0.50 and 0.75 % (w/w) was added directly to the surimi paste. Then to prepare surimi gel, first, the samples were placed in a water bath at 25 ° C for 3 hours for setting and then cooked at 90 ° C for 20 minutes. After that, the prepared gels were cooled in iced water immediately. The produced paste and gel were evaluated in terms of chemical composition, water holding capacity and color factors. Texture and sensory characteristic were assessed on the produced gels of both types of fish and compared with the control sample (without gum). Results and discussion: According to the results, the yield of Lizardfish was 28% and Talang Queenfish was 22%. It seems that the larger size of Lizardfish has been effective in its higher yield than Talang Queenfish. Based on the results, the percentage of ash, protein and fat in the treatments did not change significantly compared to the control sample. Also, there was no significant difference between the two types of fish in the amount of mentioned factors (p>0.05). As the contraction level of Konjac gum increased, the amount of moisture and water holding capacity of the paste samples of both fish increased significantly, which is due to the absorption and binding of water by the Konjac hydrocolloid. The lightness level (L*) of the surimi paste and gel of both types of fish increased significantly with the addition of Konjac, which is related to the increase in water content in the samples. The lightness of the surimi paste and gel of Lizardfish was higher than that of Talang Queenfish, which is due to the presence of more pigments in the Talang Queenfish meat. Control Sample and treatment of 0.25% of Talang Queenfish surimi paste showed more yellowness and redness than Lizardfish, but in the resulting gel, their values were reduced, indicating that the formation of gel structure by Konjac gum and binding to the proteins covered the yellowness and redness of the samples. By increasing the concentration of Konjac in surimi gel of both fish the parameters of texture (firmness, cohesiveness, adhesiveness, springiness, chewiness) breaking force and deformation increased significantly compared to the control sample. Also, the results showed that the surimi gel from Lizardfish has higher firmness, cohesiveness and strength than Talang Queenfish. Sensory evaluations showed that the panelists assigned the highest score for the surimi gel from Lizardfish to the 0.5% treatment and for the Talang Queenfish to the 0.75% treatment of Konjac. Based on the findings of the present study, it was found that surimi gel from Talang Queenfish has a lower quality than Lizardfish, but with the addition of Konjac gum, its properties can be improved.
