نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیمی مواد غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

2 گروه بیوشیمی، دانشگاه والنسیا، اسپانیا.

چکیده

در این پژوهش تاثیر هیدرولیزآنزیمی پروتئین‌گرده‌گل توسط آنزیم آلکالاز بر خواص‌آنتی‌اکسیدانی و بازدارندگی آنزیمACE بررسی و با ژله‌رویال مقایسه‌گردید. ترکیبات فنلی، فعالیت‌مهارکنندگی‌رادیکال‌DPPH، قدرت احیاکنندگی‌گرده‌گل و ژله‌رویال اندازه‌گیری شد که مقادیر آنها برای غلظت 1000 میلی‌گرم برلیترگرده‌گل و ژله‌رویال، به‌ترتیب 174 و 71/1031 میلی‌گرم اسیدگالیک برگرم نمونه، 33/67% و 27/95% و جذب 8/0 و 77/0 در طول موج 700 نانومتر بود. بیشترین قدرت احیاکنندگی تیمارهای هیدرولیز شده با آنزیم آلکالاز 5/1% و مدت زمان هیدرولیز 5/2 ساعت، 756/0 بود. بیشترین قدرت مهار رادیکال DPPH تیمارهای هیدرولیز شده با آنزیم آلکالاز 1% و مدت زمان هیدرولیز 4 ساعت، 48/78% بود. بیشترین قدرت مهار آنزیم مبدل آنژیوتنسین (ACE) تیمارهای هیدرولیز شده با آنزیم آلکالاز5/1% و مدت زمان هیدرولیز 4 ساعت 07/87% بود. نتایج نشان داد که با انجام عمل هیدرولیز، قدرت مهار رادیکالDPPH و آنزیم ACE گرده گل به‌ترتیب از 33/67% و 54/15% به 48/78% و 07/87 % ارتقا پیدا کرد. بنابراین بعد از انجام عمل هیدرولیز، قدرت مهار رادیکال و آنزیم ACE گرده‌گل، از نظر عددی نسبت به ژله رویال افزایش پیدا‌کرد. با توجه به اینکه تنها منبع اصلی پروتئینی زنبور برای تهیه ژله رویال، گرده گل است، می‌توان گفت که با هیدرولیز شدن پروتئیین‌های گرده گل، تا حدی می‌توان آنها را به پپتیدهای موجود در ژله رویال نزدیک کرد.

کلیدواژه‌ها

Almeida, J. F., Reis, A. S., Heldt, L. F. S., Pereira, D., Bianchin, M., Moura, C., Plata-Oviedo, M. V., Haminiuk, C. W.I., Ribeiro, I. S., Luz, C. F. P. And Carpes, S. T. 2016. Lyophilized bee pollen extract: A natural antioxidant source to prevent lipid oxidation in refrigerated sausages. LWT - Food Science and Technology, 71, 1-7.
Arabshahi, S., Ardestani, A., Yazdanparast, R. 2001. Quantitative assessment of antioxidant properties of natural colorants and phytochemicals: carotenoids, flavenois, phenols and indigoids. The role of B-carotene in antioxidants functions. Journal of Science of Food and Agriculture, 81, 559- 568.
Bogdanov, S. 2014. Royal Jelly, Bee Brood: Composition, Health, Medicine: A Review. Bee Product Science, 28 (3), 118-153.
Bougatef.A; Hajji.M; Balti.R. 2009. Antioxidant and free radical – scavenging activities of smoth hound muscle protein hydrolysates obtained by gastro intestinal proteases.journal of food chemistry, 1198-1255.
Coscueta, R. F., Amorim, M. M., Voss, G. B., Nerli, B. B., Pico, G. A., Pintado, M. A. 2016. Bioactive properties of peptides obtained from Argentinian defatted soy flour protein by Corolase PP hydrolysis. Food Chemistry, 198, 36–44.
Daoud, A., Malika, D., Bakari, S., Hfaiedh, N., Mnafgui, K. Kadri, A., Gharsallah, N. 2015. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date PalmPollen (DPP) from two Tunisian cultivars. Arabian Journal of Chemistry, 48, 437-447.
Deshpande, S., Chryan, M., Salunkhe, D. 1987. Tanin analysis of food products. Critical review in food nutrition, 24, 41- 49.
Guerar, F., Guimas, l., Binet, A. 2002. Production of tuna waste hydrolysates by a commercial neutral protease preparation. Journal of Molecular catalysis B: Enzymatic, 19, 489-498.
Guo, H., Ekusa, A., Iwai, K., Yonekura, M., Takahara, Y., Morimatsu, F. 2009. Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo. Journal of Nutural Science and Vitaminology, 54, 191–195.
Guo, H., Kozuma, Y., & Yonekura, M. 2005. Isolation and properties of antioxidative peptides from water-soluble royal jelly protein hydrolysate. Food Science Technology Research, 11, 222–230.
Hmidet, N., Balti, R., Nasri, R., Sila, A., Bougatef, A., Nasri, M. 2011. Improvement of functional properties and antioxidant activities of cuttlefish (Sepia officinalis) muscle proteins hydrolyzed by Bacillus mojavensis A21 proteases. Food Research International, 44, 2703-2711.
Je, J. Y., Lee, M. H., Lee, K. h. Ahn, C. B. 2009. Antioxidant and hypertensive protein hydrolysates produced from tuna liver by enzymatic hydrolysis. Food Research International, 42, 1266-1272.
Kawashima, K., Itoh, H., Miyoshi, M., & Chibata, I. 1979. Antioxidant properties of branched-chain amino acid derivatives.Chemical Pharmacological Bulletin, 27, 1912–1916.
Khantaphant, S., Benjakul, S. 2008. Comparative study on the proteases from fish pyloric caeca and the use for production of gelatin hydrolysate with antioxidative activity. Comparative Biochemistry and Physiology, 151, 110-115.
Kishimura. H., Benjakul.S. 2011. Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle nof brownstripe red snapper prepared using pyloric caeca and commercial proteases. Process Biochemistry, 318-327.
Klompong, V., Benjakul. S., Kantachote, D., Shahidi, F. 2007. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally. Food chemistry, 102 (13), 17-27.
Kroyer. G., Hegedus, N. 2001. Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innovative Food Science & Emerging Technologies, 2, 171-174.
Ktari, N., Nasri, R., Mnafgui, K., Hamden, K., Belguith, O., Boudaouara, T., Feki, A., Nasri, M. 2014. Antioxidative and ACE inhibitory activities of protein hydrolysates from zebra blenny (Salaria basilisca) in alloxan-induced diabetic rats, 5113 (14), 73-77.
Lassoued, I., Mora, L., Nasri, R., Aydi, M., Toldra, F., Aristoy, M., C., Barkia, A., Nasri, M. 2015. Characterization, antioxidative and ACE inhibitory properties of hydrolysates obtained from thornback ray (Raja clavata) muscle. Journal of Proteomics.
Leblanc, B. W., Davis, O. K., Boue, S., DeLucca, A., Deeby, A. 2009. Antioxidant activity of Sonoran Desert bee pollen. Food Chemistry, 115, 1299–1305.
Liu, J. R., Yang, Y. C., Shi, L.S., Peng, C. C. 2008. Antioxidant properties of royal jelly associated with larval age and time of harvest. Journal of Agriculture and Food Chemistry, 56 (23), 11447-11452.
Mao, W. Y., Cheng, X., Wang, X., Wu, S. I. 2011. Free-radical-scavenging and anti-inflammatory effect of yak milk casein before and after enzymatic hydrolysis. Food Chemistry, 126 (2011), 484–490.
Marghitas, L. A., Stanciu, O. G., Dezmirean, D. S., Bobis, O., Popescu, O., Bogdanov, S., Campos, M. S., 2009. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chemistry, 115, 878–883.
Marinova, M., Tchorbanov, P. 2010. Preparation of Antioxidant Enzymatic Hydrolysates from Honeybee-Collected Pollen Using Plant Enzymes. Enzyme Research, 41, 5949-50.
Matsuoka,T., Kawashima,T., Nakamura, T.,Kanamaru,Y., Yabe, T. 2012. Isolation and characterization of proteases that hydrolyze royal jelly proteins from queen bee larvae of the honeybee, Apis mellifera. Apidologie, 43, 685–697.
Morais, M., Moreira, L., Feas, X., Estevinho, L.M. 2011. Honeybee-collected pollen from five Portuguese natural parks: Palynological origin phenolic content antioxidant properties and antimicrobial activity. Food and Chemical Toxicology, 49, 1096-1101.
Nagai, T., Inoue, R., Suzuki, N., Myoda, T., Nagashima, T. 2005. Antioxidative ability in a linoleic acid oxidation system and scavenging abilities against active oxygen species of enzymatic hydrolysates from pollen Cistus ladaniferus. International Journal of Molecular Medicine, 15(2), 259-63.
Nagai, T., & Inoue, R. 2004. Preparation and the functional properties of water extract and alkaline extract from royal jelly.Food Chemistry, 84, 181–186.
Nasri, R., Younes, I., Jridi, M., Trigui, M., Bougatef, A., Arroume, N., Dhulster, P., Nasri. M., Châabouni, M.K., 2013. ACE inhibitory and antioxidative activities of Goby (Zosterissessor ophiocephalus) fish protein hydrolysates: Effect on meat lipid oxidation. Food Research International, 54, 552–561.
Pascoal, A., Rodrigues, S., Teixeira, A., Feas, X., L, M. Estevinho. 2013. Biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidant and anti-inflammatory. Food and Chemical Toxicology.
Salampessy, J., Reddy, N., Kailasapathy, K., Phillipsa, M. 2015. Functional and potential therapeutic ACE-inhibitory peptides derived from bromelain hydrolysis of trevally proteins. Journal of Functional Foods, 14, 716-725.
Sun, L., Powers, J. R., and Tang, J. 2007. Evaluation of antioxidant activity of asparagus, broccoli and their juices. Food Chemistry, 105, 101- 106.
Villanueva, A., Vioque, J., Sanchez-Vioque, R., Clemente, A., Pedroche, J., Bautista, J., Millan, F. 1999. Peptide Characteristics of Sunflower Protein Hydrolysates. Journal of the American Oil Chemists Society, 76, 1455-1460.
Wiriyaphan, C., Chitsomboon, B., Yongsawadigul, J. 2012. Antioxidant activity of protein hydrolysates derived from threadfin bream surimi byproducts. Food Chemistry, 132, 104-111.
Zhang, M., Mu, T.-H., & Sun, M.-J. (2014). Purification and identification of antioxidant peptides from sweet potato protein hydrolysates by Alcalase. Journal of Functional Foods, 7, 191–200.
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