Antibiotic resistance pattern and frequency of ESBL producing Enterobacteriaceae isolated from lettuce and spinach in Gorgan

Document Type : Research Article

Authors

• Anegol Kheradmand ¹
• Ania Ahani Azari ¹
• Ahmad Danesh ²

¹ Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran.

² Department of Health and Social Medicine, School of Medicine, Golestan University of Medical Sciences, Iran.

https://doi.org/10.22067/ifstrj.v1396i0.62853

Abstract

Introduction: In recent years, there have been many epidemic outbreaks caused by consumption of contaminated vegetables. In most cases, Enterobacteriaceae were responsible for these epidemics. The purpose of this study was to investigate antibiotic resistance pattern and frequency of strains producing broad-spectrum beta-lactamase (ESBL) isolated from lettuce and spinach produced in Gorgan, Iran.

Material and Methods: After culturing the isolates on MacConkey medium, colonies with specific morphological characteristics were selected. After preparation of pure culture, strains resistant to cefotaxime were identified and strains belonging to the Enterobacteriaceae family were isolated. The isolates were then studied for broad-spectrum beta-lactamases (ESBL) and their antibiotic resistance patterns were determined.

Results & Discussion: The results of this study showed that ESBL Enterobacteriaceae is present in vegetables such as lettuce and spinach. It was also found that all isolated ESBL Enterobacteriaceae from spinach samples were resistant to Cotrimoxazole, Nalidixic Acid, Tetracycline, Chloramphenicol, and Amoxicillin, and sensitive to Imipenem. Isolated ESBL Enterobacteriaceae from lettuce samples was sensitive to Amikacin and Imipenem. Multiple antibiotic resistance was observed in all isolates. Therefore, it can be concluded that use of animal fertilizers increases the risk of antibiotic-resistant bacteria in vegetables and ultimately in humanand animals.

Keywords

• Enterobacteriaceae
• broad-spectrum beta-lactamases
• Vegetables
• antibiotic resistance

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