Document Type : Research Article

Authors

1 Horticulture Group, Agriculture and Natural Resources College, Hormozgan University, Bandar Abbas, Iran.

2 Department of Packaging Engineering, Agricultural Engineering Research Institute (AERI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Introduction: Persian walnut (Juglans regia) from Juglandaceae family is one of the most important fruits in the world that has an important role in human health. It contains a notable list of plant nutrients that has been found to have disease preventing and health promoting properties. Walnut is a source of bioactive compounds such as phenolic and flavonoid complexes where they act as antioxidants and free radical scavengers. It also contains pleasant flavor and high concentration of minerals and fatty acids. Extending shelf-life of this perishable fruit has been accomplished due to its high oil level and oxidation of oil (Tajeddin, 2004). Rate of loss in walnut kernel quality and quantity between harvest and consumption affects its productivity. Control of temperature is the most effective tool for extending the shelf life of fresh horticultural products. However, to reduce high losses and keeping product’s quality, packaging method is very important as well as low temperature. Therefore, in this study, the effect of temperature (4 and 25 oC) and packaging methods (vacuum and air packaging) on the changes of walnut kernel compounds was evaluated to improve quality of its storability during six month cold storage.

Materials and methods: Walnut harvested from a commercial garden at the mountain regional of Raber, Kerman province. All chemical materials for different tests obtained from Merck Company, Germany. Immediately after walnuts harvesting, they were dehulled and dried in expose of sun shade with the circulation of natural air. The walnuts were then transferred to the laboratory and their wooden shells were removed. Subsequently, about 25 g of walnut kernel was packaged in the polyethylene films with 87μm (0.087 mm) thickness, under a vacuum machine and stored at 4°C and 25°C to be later assessed for further analyses intended for six months. Phenol, flavonoid, carbohydrate, protein, water percent, color parameters (C, h, WI), organoleptic characteristics, and peroxide value of kernels were measured every month during storage time. The control samples of packaged walnuts under environmental conditions were also stored. The current study carried out as a factorial assay on the basis of a completely randomized design with three replications at Hormozgan University. Data were subjected to ANOVA using SAS software version 9.4. Verification of significant differences was done using Duncan's Test at 1% probability level.

Results and discussion: Results showed that carbohydrate and proteins decreased during storage time. Both of vacuum package and low temperature contorted the reduction of different characteristics of kernel such as bioactive compounds during storage significantly. Sensory properties were also reduced during storage, especially at the end of period, in all conditions except for treatment at 4°C and vacuum packing. Control samples (temperature 25°C and air-containing packags) during the experiment showed a lower quality for all factors. Samples that stored in low temperature and vacuum package had better brightness (higher chroma, Hugh, lightness (L) and white index values (Wi)) than other treatments. The treatments had a significant role in the preventing of increasing peroxide value. The peroxide value in treated samples was increased from 0.023 to 0.68 meq/g, while in vacuum packages it was changed from 0.023 to 0.37 meq/g. Increasing of peroxide value was observed from 0.023 to 0.68 and from 0.023 to 0.25 meq/g in room temperature and cold temperature, respectively. After six months, the average peroxide value in all samples was less than one milliequivalent per oil kilogram. Decreasing of phenolic compounds (30%) and flavonoids (35%) and increasing the peroxide index simultaneously led to the reduction of the appearance and organoleptic properties of the control samples. Generally, vacuum package and low temperature condition that using in this study showed the best effect on the nutritional compounds quality of walnut kernel such as bioactive components during six months storage. Shelf life enhancement of walnut by vacuum packaging in the different polymers has been already reported by Tajeddin, 2004.

Keywords

تاج الدین، ب. 1383. بررسی اثر پوشش‌های پلیمری در بسته‌بندی مغز گردو. فصلنامه علمی - پژوهشی پژوهش و سازندگی، جلد 17، شماره 1، 8-3.
حمیدی، ش.، یزدانی، ن.، رضایی، ک.، فرجی، ر. و وحدتی، ک. 1394. ‌ارزیابی ویژگی‌های فیزیکی شیمیایی و پایداری اکسایشی مغز گردو (Juglan regia L.) با رنگ‌های زرد، کهربایی و قهوه‌ای. مجله مهندسی بیوسیستم ایران، 46(3) ، 285-275.
Bakkalbaşi, E., Ö.M., Yilmaz, O. Yemiş, and N.Artik. (2013). Changes in the phenolic content and free radical-scavenging activity of vacuum packed walnut kernels during storage. Food Science and Technology Research, 19(1):.105-112.
Bradford, M.M. A., (1976). rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72:248-254.
Chen, Q., Li, L. and Wu, Y., (2004). Progress of research on the chemical components and pharmaceutical action of walnut kernel. Journal of Anhui University Natural Sciences, 29(1), :86-89.
Colaric, M., Veberic, R., Solar, A., Hudina, M., & Stampar, F.(2005). PHenolic acids,syringaldehyde, and juglone in fruits of different cultivars of Juglans regia L. Journal.Agricultural. Food Chemistry. 53, 6390–6396.
Christopoulos, M..V and Tsantili, M.V., (2011). Effects of temperature and packaging atmosphere on total antioxidants and colour of walnut (Juglans regia L.) kernels during storage. Scientia Horticulturae, 49–57
Christopoulos, M.V., Tsantili, E., Papageorgiou, V., Komaitis, M., and Rouskas, D., (2010). Effects of package atmosphere and temperature on phenolics, total antioxidant capacity and colour in kernels of ‘Franquette’ walnuts during 8-month storage. Acta Hortic. 858, 75–81.
Dong, W.L., Gong, X., Sun, Z.H., Dong, J. and Jin, L., (2014). Research of quality impact of different packaging manage on peanut storage. Chemical Engineer, 3, p.020.
Dubis , M., K. A., Gilles, J. K., Hamilton,P.A., Rebers and Smith, F., (1956). Colometric method for determination of sugar and related substances. Analytical Cgemistry, 28: 350-356.
FAOSTAT., 2011, Food and Agriculture Organisation, Rome
Fatemi, H. 2003. Food Chemistry. 2nd Edn. Sahami Compony of Enteshar.Tehran. 481p (In Persian).
Ghirardello, D., Contessa, C., Valentini, N., Zeppa, G., Rolle, L., Gerbi, V. and Botta, R., (2013). Effect of storage conditions on chemical and physical characteristics of hazelnut (Corylus avellana L.). Postharvest Biology and Technology, 81:37-43.
Horwitz, W., Senze, A., Reynolds, H. and Park, D.L., (1975). Official methods of analysis of the association of anaytica1 chemists. Washington: Associat Official Analytic Chemist.
Hosseini, H., Ghorbani, M., Sadeghi, A. R, and Maghsoudlou,Y., (2012) the effect of contact with the atmosphere and ambient temperature storage on the Oxidative Stability of Walnut. Journal of Food Science and Technology of preceding studies. 9: 358-348
Hotchkiss, J. H., (1988) Experimental approaches to determining the safety of food packaged in modified atmospheres. Food Technology, 42(9), 55-64.
Jensen,P.N., Sørensen,G.,Brockhoff, P. and Bertelsen, G.,(2003). Investigation of packag-ing systems for shelled walnuts based on oxygen absorbers. J.Agric. Food Chem.51: 4941–4947.
Johnson, D.R. and Decker, E.A., 2015. The role of oxygen in lipid oxidation reactions: a review. Annual review of food science and technology, 6: 171-190.
Kacyn, L. J., Saguay, I. and Karel, M., (1983) Kinetics of oxidation of dehydrated food at low oxygen pressures. Journal of Food Processing and Preservation, 7(3), 161-178.
Kader, A.A., Thompson, J.E.(2002). Postharvest handling systems: tree nuts. In:Kader, A. (Ed.), Postharvest Technology of Horticultural Crops. University of California, Division of Agriculture and Natural Resources, Oakland, pp. 399–406.
Manzocco, L., Calligaris, S., Mastrocola, D., Nicoli, M.C., and Lerici, C.R., (2000) Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci. Technol. 11, 340–346.
McGuire,R.G.,(1992) Reporting of objective color measurements. HortScience 27, 1254–1255.
Mitcham, E. J., Veltman, R. H., Feng, X., and Castro, E., et al., (2004) Application of radio frequency treatments to control insects in in‐shell walnuts. Postharvest Biol. Technol, 33:93–100.
Necla, C.(2003). Biochemical and physical properties of some walnut genotypes (Juglans regia L). Nahrung/Food 47No.1, pp. 28–32.
Penter, M.G., Bertling, I. & Sippel, A.D., (2014) August. Factors affecting shelf life of South African macadamias. In XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): 1109 (pp. 11-16).
Piccirillo, P., Fasano, P., Mita, G., De Paolis, A. & Santino, A., (2004) November. Exploring the role of lipoxygenases on walnut quality and shelf-life. In V International Walnut Symposium 705: 543-545.
Rabrenovic, B., Picuric-Jovanovic, K., & Sobajic, S. (2008). Physicochemical properties and fatty acid composition of Juglans regia cultivars grown in Serbia. Chemistry of Natural.
Raisi, M., Ghorbani, M., Mahoonak, A.S., Kashaninejad, M. & Hosseini, H., (2015). Effect of storage atmosphere and temperature on the oxidative stability of almond kernels during long term storage. Journal of Stored Products Research, 62, :.16-21.
Savage, G. P. & McNeil, D. L, (2001). Oxidative stability of walnuts during long term in shell storage. Acta Hortic., 544, 591-597.
Shui G and Leong LP.(2002). Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by highperformance liquid chromatography Journal. Chromatogr
Talcott, S.T., Duncan,C.E., Pozo-Insfran, D.D. & Gorbet, D.W.,(2005). Polyphenolic and antioxidant changes during storage of normal,mid, and high oleicacid peanuts. Food Chem.89, 77–84.
Young, C. K. & Cunningham, S. (1991). Exploring the partnership of almonds with cereal foods. Cereal Foods World, 36(5), 412-418.
Vanhanen, L. P. & Savage, G. P., (2006). The use of peroxide value as a measure of quality for walnut flour stored at five different temperatures using three different types of packaging. Food Chemistry, 99(1), 64-69.
Vasudevan, S.N., Shakuntala, N.M., Teli, S., Goud, S. & Gowda, B., (2014). Studies on effect of modified atmospheric storage condition on storability of groundnut (Arachis hypogaea L.) seed kernels. International Journal of Research Studies in Biosciences, 2(2), pp.25-36.
Wang, S., Monzon, M., Johnson, J.A., Mitcham, E. J. & Tang, J., (2007). Industrial-scale radio frequency treatments for insect control in walnuts.II: heating uniformity and energy efficiency. Postharvest Biol. Technol.45, 240–246.
Zhi, H.B. & Han, Y.S., (2006. Study on Inhibiting of Walnut Rancidity. Packaging Engineering, 5, p.009.
Sze-Tao, K.W.C.; Sathe, S.K. Walnuts (Juglans regia L): proximate composition, protein solubility, protein amino acid composition and protein in vitro digestibility. 2000. J. Sci. Food Agr. 80, 1393–1401.
CAPTCHA Image