نوع مقاله : مقاله پژوهشی فارسی
نویسندگان
گروه علوم و صنایع غذایی، دانشگاه آزاد اسلامی، واحد دامغان، دامغان، ایران.
چکیده
عصاره های گیاهی دارای خاصیت ضدمیکروبی بوده و میتوانند به‌عنوان یک طعم‌دهنده، جایگزین نگهدارنده های شیمیایی در مواد غذایی شوند. در این پژوهش اثرهم افزایی خاصیت ضدمیکروبی ترکیب عصاره های هیدروالکلی سه گیاه ریحان، دارچین و مریم گلی بر روی سویه های غذایی اشریشیاکلای، استافیلوکوکوس اورئوس و مخمر ساکارومایسس سرویزیه در محیط دوغ در طی 4 هفته نگهداری در دمای محیط بررسی شد. نتایج حاصل از این پژوهش نشان داد، حداقل غلظت مهارکنندگی عصاره های مورد آزمایش به‌صورت تکی بین 60/1 تا 5/12 میلی گرم بر میلی لیتر و حداقل غلظت ممانعت کنندگی ترکیب هر سه عصاره بین 4/0 تا 12/3 میلی گرم بر میلی لیتر بود. همچنین ترکیب هر سه عصاره ریحان، دارچین و مریم گلی بر علیه هر سه باکتری اثر هم افزایی داشت و در تمام حالات ترکیب عصاره ها اثرات رقابت کنندگی مشاهده نشد. ترکیب هر یک از عصاره های فوق‌الذکر با غلظت 62/0 میلی گرم بر میلی لیتر با یکدیگر، به‌صورت معنی داری منجر به کاهش جمعیت میکروبی به ویژه باکتری های گرم مثبت شد (05/0p<). همچنین پذیرش کلی دوغ حاوی ترکیب هر سه عصاره مورد بررسی قابل قبول ارزیابی شد و حدود 90 درصد شرکت‌کنندگان حاضر به مصرف آن بودند. بنابراین کاربرد همزمان این عصارهها میتواند در کنترل مؤثر بیماری‌زاهای مواد غذایی به‌عنوان یک روش مکمل مورد استفاده قرار گیرد.
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