Effect of black cumin essential oil on physicochemical, microbial and sensorial characteristics of ultrafilterated Feta cheese

Document Type : Full Research Paper

Authors

• Marziyeh Momeni Sarvestani ¹
• Hannan Lashkari ²

¹ Department of Food Science and Technology, Islamic Azad University, Sarvestan Branch, Iran, Sarvestan.

² Department of Food Science and Technology, Islamic Azad University, Zarrin Dasht Branch, Iran, Zarrin Dasht.

https://doi.org/10.22067/ifstrj.v16i4.80664

Abstract

Introduction: Black cumin is one of the most important and valuable herbal plants that are wildly found in regions of Iran with dry weather such as Kerman, Fars, Isfahan and Yazd. Black cumin seed in traditional medicine is used as anti- flatulence, anti-diarrhea, antipyretic, blood fat and cholesterol reducer, anti-allergic and digestive enhancers. Essential oils are oily liquids that are obtained from different parts of the plant. Different compounds in the essential oil of black cumin include cumin-aldehyde, gamma trypinene, paracymon and other active ingredients, which are often terpene compounds and have numerous uses as anti-bacterial and antioxidant agents. In recent years, consumers have become more concerned with the health issues and are looking to eat foods with higher functional characteristics, as well as higher nutritional value. Therefore, it can be suggested that essential oil of Cumin as a natural and vegetable preservative was used in food. Because cheese is a nutritious environment for the growth of microbes, it always has microbial degeneration.
 
Materials and Methods: Identification of essential oil compound was performed by GC/MS chromatography. In order to evaluate the effect of Black Cumin essential oil on physicochemical, microbial and sensory properties of cheese, different percentages of Black Cumin essential oil (0, 0.05, 0.1% and 0.15%) were added to cheese. Iranian white cheese production was done by ultrafiltration method in Arjan Fars Company. Cheese sample were analyzed at days 3, 30 and 60 of storage time. Acidity, pH, fat, protein and dry matter were measured according to National Standard No. 2852, 2852, 8785, 1811, 1753 respectively. To measure the inhibition of free radicals of DPPH, method of Rezai et al(2012) was followed. Microbial characteristics were performed according to the standard microbial milk and dairy products number 2406. The color analysis was performed based on Hosseini et al (2019) method. The parameters of color include L* (lightness), a* (redness) and b* (yellowness) were measured. The sensory attributes were evaluated by 9 panelists. A five-point hedonic scale rating (1= very bad, 2=bad, 3= neither bad nor good, 4= good, 5= very good) was carried out. Data analyzed with SPSS: 22 software and Means were compared with Duncan multiple range test (p<0.05).
 
Results and Discussion: The analysis of black cumin essential oil with GC/MS chromatography showed that the main ingredients of it were α-Thujene, p-Cymene, Linalool, Thymoquinone, Carvacrol and Longifolene. The results showed that Changes in essential oil percentage had significant effect (p<0.05) on acidity, pH, IC50. With increasing essential oil percentage, an increase in the level of acidity and decrease in the level of IC50 and pH was observed. Changes in essential oil percentage had no significant effect(p<0.05) on protein, fat, salt and dry matter content of cheese. The results showed that storage period had no significant effect (p<0.05) on protein, fat and salt content. Increasing the essential oil percentage of cheese significantly decreased the L* index and increased the b* index. The microbial analyses showed no microbial contamination (coliforms, sataphylococcus aureus, mold and yeast) was found during the storage time therefore, the essential oil of Cumin can be used as a natural preservative in cheese production. Analyses of the sensory characteristics during storage time showed that the addition of essential oil to cheese did not have any significant effect on color and texture but had a significant effect on taste and acceptance of cheese. In all treatments, the taste score changed significantly with time (p <0.05). Over time, the flavour rating began to increase until the twentieth day, and then decreased to the end of the storage time. In general, the treatment containing 0.05% Black Cumin essential oil has the highest acceptance in sensory evaluation and since its other characteristics were standard, it was selected as the best treatment.

Keywords

• Black cumin
• Cheese
• Essential oil
• Physicochemical
• microbial

References

Abdel-Razig, K.A., Ahamed Kunna, M. and Mohammed, A.S. (2014). Effect of levels of black cumin seeds (Nigella sativa) and storage period on biochemical properties and acceptability of Sudanese braided cheese. Sudanese Journal of Agricultural Sciences, 1: 55–62.

Abduganiew, B.E., Abdullaev, U.A., Aripov, K.N., Baser, K.H.C. and Oezek, T. (1997). Compositionof theessential oil of Bunium persicum (Boiss.) B. Fedtsch. from Tajikistan. Journal of Essential Oil Reserch, 9: 597 - 8.

Ahmed Hamid, O.I. (2014). Effect of cumin oil concentrations on chemical composition and sensory characteristics of Sudanese white cheese during ripening. International Journal of Current Microbiology and Applied Sciences, 3 (4): 961-968.

Akbarpour J. (2017) evaluation and study the effects of Althaea officinalis extract and Tragacanth Gum as a fat replacer on physichochemilchal properties of probiotic yogurt. MSc thesis.

Alsawaf, S.D. and Alnaemi, H. S. (2011). Effect of Nigella sativa (seed and oil) on the bacteriological quality of soft white cheese. Iraqi Journal of Veterinary Sciences, 25 (1): 21-27.

Ataie Kachouei M.(2016). Study the antimicrobial effects of the essential oils of Origanum vulgare, Mentha piperita and Carum carvi on the bacteria isolates from food stuffs. Journal of food microbiology. 3: 1-10.

Bourgou, S., Pichette, A., Marzouk, B., & Legault, J. (2010). Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany, 76(2), 210-216.

Burt, S. (2004). Oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology, 94 (3): 223–253.

Dehkhoda, A.A. (1975). Dictionary of Persian words, (In Persian).University of Tehran press. Tehran. 264 - 275.

Fox, P.F. (1993). Cheese: Chemistry, Physics and Microbiology. Chapman and Hall, London, 1: 414.

Gahruie, H. H., Eskandari, M. H., Van der Meeren, P., & Hosseini, S. M. H. (2019). Study on hydrophobic modification of basil seed gum-based (BSG) films by octenyl succinate anhydride (OSA). Carbohydrate polymers, 219, 155-161.

Ghods Rohani M. Mortazavu A. Mazaheri Tehrani M. (2010). Evaluation the effects of shelf life on physical, chemichal and sensorial properties ultrafiltreted UF feta chease.Iranian Food Science and Technology Research Journal. 6, 3, 149-157.

Gholamzadeh M ؛ Hosseini H ؛ Eskandari S ؛ Hosseini؛ E Gholamzadeh M (2013). Antioxidant activity of black cumin (Nigella sativa L.) and black caraway (Buniumpersicum Boiss) extracts, individually and in combination on chemical changes and sensory properties of silver carp (Hypophthalmichthysmolitrix) stored in refrigerator. 3 (11); 11-22.

Hashemi Gahruie, H., Hosseini, S. M. H., Taghavifard, M. H., Eskandari, M. H., Golmakani, M.-T., & Shad, E. (2017). Lipid oxidation, color changes, and microbiological quality of frozen beef burgers incorporated with Shirazi thyme, cinnamon, and rosemary extracts. Journal of Food Quality, 2017.

Amraei, H., Azizi, M.H., Barzegar, M., and Ahmadi Ghavlighi, H. (2007). Chemical and sensory characteristics of UF white cheese fortified with iron. J. Agric. Sci. Natur. Resour. 14(4), 1-9.

Haghiroalsadat F, Vahidi A, Sabour M, Azimzadeh M, Kalantar M, Sharafadini M. (2011) The Indigenous Cuminum Cyminum L. of Yazd Province: Chemical Assessment and Evaluation of its Antioxidant Effects. 19 (4) :472-481

Hajhashemi, V., Ghannadi, A., & Jafarabadi, H. (2004). Black cumin seed essential oil, as a potent analgesic and antiinflammatory drug. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 18(3), 195-199.

Hosseini, Z. (1998). Current methods in food stuff analysis. 3rd ed, shiraz, shiraz university press, 52-53.

Jahan, I.I. and Abdel-Hakim, E.H. (2015). Quality improvement of Damietta cheese usings some spices extract. Assiut Veterinary Medical Journal, 61 (147): 24-33.

Kamkar A, Khanjari A, Oladi M, Molaee Aghaee E. (2017) Effect of packaging with chitosan film containing Bunium persicum L. essential oil on chemical and microbial properties of chicken fillet. J Fasa Univ Med Sci. 7 (1) :104-115

Karim G. (1997). Milk and Dairy product. First ed. University of Tehran Press. Iran. 206 - 237.

Pourmortazavi, S.M., Ghadiri, M. and Hajimirsadeghi, S.S. 2005. Supercritical fluid extraction of volatile components from Bunium persicum Boiss. (black cumin) and Mespilus germanica L. (medlar) seeds. Journal of Food Composition and Analysis, 18: 439 - 446.

Pour-seyedi, S. (1994). Assessment of germination and cytology of three Iranian caraway genus: Bunium, Carum and Cuminum. Tehran: University of Tehran. 89. [persian].

Rafei؛ S. Azizkhani؛ M. Areaei P. (2017). Impact of Antioxidative Properties of Cumin and Tarragon Essential Oils on the Quality of Full-Fat White Cheese. 14; 79-90.

Ramadan Hassanien, M.F., Mahgoub, S.A. and El-Zahar, K.M. (2014). Soft cheese supplemented with black cumin oil: Impact on food borne pathogens and quality during storage. Saudi Journal of Biological Sciences, 21: 280–288.

Reddy, S. H., Al-Kalbani, A. S., & Al-Rawahi, A. S. (2018). Studies On Phytochemical Screening-Gc-Ms Characterization, Antimicrobial And Antioxidant Assay Of Black Cumin Seeds (Nigella Sativa) And Senna Alexandria (Cassia Angustifolia) Solvent Extracts. International Journal Of Pharmaceutical Sciences And Research, 9(2), 490-497.

Rezaie Erami S. Jafari Sm. Khomeiri M. Bayat. H. (2012). Comparison of antioxidant properties Walnut leaf extract extracted from convectional and microvave assisted extraction. Iranian Food Science and Technology Research Journal. 8, 2, 219-234.

Sadeghi E, Akhondzadeh Basti A, Misaghi A, Zahraii Salehi T, Bohlouli Osgoii S. (2010) Evaluation of effects of Cuminum cyminum and Probiotic on Staphylococcus aureus in Feta Cheese. JMP. 2 (34) :131-141

Shankaracharya, N.B. and Shankaracharya, M.L. (1988). Research note on the essential oils of Cuminum cyminum L. and Bunium persicum. B. Pafai Journal, 10 (4): 33-35.

Soleymani N, Sattari M, Sepehriseresht S, Daneshmandi S, Derakhshan. (2010) Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. Iran J Med Microbiol.; 4 (1 and 2) :26-34

Taherkhani P, Noori N, Akhondzadeh Basti A, Gandomi H, Alimohammadi M. (2015) Antimicrobial Effects of Kermanian Black Cumin (Bunium persicum Boiss.) Essential Oil in Gouda Cheese Matrix. JMP. 2 (54) :76-85

Tarakci, Z., Temiz, H. and Ugur, A. (2011). The effect of adding herbs to labneh on physicochemical and organoleptic quality during storage. International Journal of Dairy Technology, 64 (1): 108-116.

Zeinali, N. (2007). Recognition, cultivation and culturing of Caraway, (In Persian). Vadiat. Kerman. 56.