Investigating the effect of operational factors on reducing cream cholesterol by beta-cyclodextrin using response surface method

Document Type : Full Research Paper

Authors

• Mitra Mansouri Bani ¹
• Vahid Samavati ²
• Marzieh Bolandi ³

¹ Department of Food Science and Technology, Kherad Institute of Higher Education, Bushehr, Iran.

² Department of Food Science and Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran.

³ Department of Food Science and Technology, Islamic Azad University, Damghan Branch, Damghan, Irani.

https://doi.org/10.22067/ifstrj.v16i4.81186

Abstract

Introduction: The Cholesterol in our diet is a risk factor for the prevalence of cardiovascular diseases. One of the effective ways to reduce the incidence of such diseases is to remove cholesterol, including animal fats, from our food sources. In this research, attempts were made to investigate the effect of operational factors on reducing cholesterol in cream by beta-cyclodextrin using RSM and to examine its physicochemical properties. The variables in this study were beta-cyclodextrin concentration, mixing temperature and mixing time.
 
Materials and Methods: The 3.5% fat raw milk was pasteurized at 72°C for 16 s, and then it was cooled to 55°C. The milk cream was separated by a separator and adjusted to 36% fat. Beta-cyclodextrin was added in 0.5, 1, 1.5% concentration in water bath at three levels of 10, 30, 50 ° C. It was mixed at three time intervals of 5, 15, 25, min. Cream containing beta-cyclodextrin was centrifuged to eliminate the complex formed in different periods and the beta-cyclodextrin and cholesterol complex was isolated. Beta-cyclodextrin of 99.2% purity was purchased from Sigma-Aldrich and all chemical agents from Merk, Germany. To measure cholesterol content in the preparation phase, hexane and ethanolic solution of potassium hydroxide (Merk) and the standard solution of cholesterol (Merk) were used. To determine the level of cholesterol in the samples, Lee et al. (1999) method was used after a few modifications. Firstly, cholesterol was extracted from the specimens and the cholesterol levels in the samples were determined with a chromatography apparatus equipped with flame ionization. The quantitative measurement of cholesterol was conducted by comparing the peak areas with a response from the internal standard. The experiments were designed, using the response surface method and the Box-Behnken Design (BBD), to achieve a high-efficiency process in reducing the highest cholesterol level and determining optimum conditions. The viscosity was determined using a Brookfield viscometer, and the over run and foam stability of the cholesterol cream samples were determined using a Graduated cylinder.
 
Results and discussion: Due to the cavity in the central part of beta-cyclodextrin molecular arrangement, it can form a stable cholesterol-insoluble complex and help to isolate it from the product. The results showed that time, mixing temperature, and beta-cyclodextrin concentration all had a positive effect on reducing the level of cholesterol, and the effect of independent factors was almost the same, as the time factor had the highest effect, supported in Makoto et al. (1999). Concentration factor had the least effect on cholesterol reduction, contrary to studies by Aryafar et al. (2007) and Yen et al. (1995). Among the interactions of the investigated factors, the highest effect was due to the simultaneous effects of temperature and time (88.9%) and the least effect was due to the simultaneous effect of concentration and temperature (85.5%). Findings of this study showed that the use of beta-cyclodextrin resulted in a successful reduction of cholesterol from the product without affecting tissue properties, increasing viscosity and volume, or reducing stability of the foam. The absorption of cholesterol by beta-cyclosporine depends on its concentration. The mixing time and temperature also boosts the effect. After examining the effects of each independent variable, it can be concluded that all three investigated variables have an incremental effect on the level of cholesterol in the evaluated levels. Increasing mixing time has the highest effect on increasing cholesterol and increasing the concentration of beta-cyclodextrin has the lowest effect on cholesterol reduction. Investigating the interaction effects of variables also showed that the simultaneous effect of temperature and time with the proper concentration of beta-cyclodextrin could have a significant effect on cholesterol reduction. The response surface method and Box-Behnken Design as statistical optimization and modeling techniques enabled the researchers to predict the optimal conditions for maximal removal of cholesterol by beta-cyclodextrin at the decided levels. The optimal operating condition obtained by the model for 36% fat cream contains 42.1% beta-cyclodextrin concentration, 76.75 ° C temperature and 87.83 min of mixing time. The predicted cholesterol reduction in these conditions was 89.92%, which was very close to the experimental value obtained in predicted optimal conditions. It indicates the accuracy and predictive power of the intended model.

Keywords

• beta cyclodextrin
• cream
• cholestrol
• Optimization
• Response surface method

References

Abuajah, C. I., Ogbnna, A. C., & Osuji, C.M. (2015). Functional Components and Medicinal Properties of Food: Areview. Journal of food Science and Technology, 52 (5), 2522-2529.

Ahn, j., Kwak, H.(1999) Optimizing cholesterol removal in cream using β_cyclodextrin and response surface methodology.journal of foodscince,64 (4), 629-632.

Alonso, L., Cuesta, P., Fontecha, J., Juarez, M., and Gilliland, S. E. (2009). Use of betacyclodextrin to decrease the level of cholesterol in milk fat, Journal of Dairy Science, 92(3): 863–869.

Amiri, S., Radi, M. (1999). Investigating the physico-chemical and texture properties of low-fat cream, prepared from starch modified wheat, 18th National Congress of Science and Technology, Iran.

Aryafar, M., Zandi, p. (2007). Low Cholesterol Butter Production By Using β-cyclodextrin. Journal of Nutrition Sciences and Iranian Food Industry, 3: Pages From 23-32.

Boudreau, A., and Arul, J. (1993). Cholesterol Reduction and Fat Fractionation Technologies for Milk Fat: An Overview, Journal of Dairy Science, 76: 1772-1787.

Bruhn, C., Bruhn, J. (1988). Observations on the whipping characteristics of cream, Journal of Dairy Science, 71: 857–862.

Dickinson, E., (1997) Propertise of emulsions stabilized with milk proteins.J. Dairy Science, 80(10): 2607-2619.

Donnell, J. A. O. 1993. Future of Milk Modification by Production or Processing: Integration of Nutrition, Food Science and Animal Science, Journal of Dairy Science, 76: 1797-1801.

Doosh, K .S.,Majeda, F.,& SO, M. A. (2013). Manufactring Cholesterol – Reduced Butter by Betacyclo dextrin and study some of its Chemical and Physical Properties. Pakistan Journal of Nutrition, 12(9), 837-841

Food Consumption Report (2000-2001). Institute of Nutrition Research and Food Industry of the Country.

Galante., M., Pavon, Y., Lazzaroni, S., Soazo, M., Costa, S., Boerris, V., & Rozycki, S. (2017). Effect of Cholestrol-reduced and Zinc fortification treatments on Physicochemical, functional, TextureMicro structural and Sensory Properties Of Soft Chees. International Journal of Dairy Technology.

German Experimental Whipping Cream Standard (1996).

Gonzalez-Hierro, M., Ruiz-Sala, P., Alonso, L., and Santamaria, G. (1995). Extraction of ewe’s milk cream with supercritical carbon dioxide, Z. Lebensm. Unters. Forsch. 200: 297–300.

Grundy, S., Brheimer, D., Blackburn, H., Brown, W., Kwiterovich, P., Mattson, F., Schonfeld, G., and Weidman, W. (1982). Rationals of the diet-heart statement of the American Heart Association. Report of the Nutrition Committee, Circulation 65: 839A–854A.

Gurr, M. (1992). Dietary lipids and coronary heart disease: old evidence, new perspective. Progress, Lipid Research. 31: 195–243.

Hariharan, K.,soma kurien, k. and venkat Roa,s.(1995). Effect of supplementaition of milk fat with peanut oil on blood lipids and lipoproteins in infants. International Journal of Food Science. Nutrrriant, 46: 309-317.

Health and Diseas Survey in Iran (2001). Publication of Secretariat of Applied Research of Health Deputy.

Hettinga D.in Baileys industrial oil and fat products. Edited by F.Shahidi, 6 th., Vol.2, john Wiley, New York; (2005), pp 15-21.

Kim SH, Ahn J, Kwak HS. Optimizing Cholesterol removal from Milk. Arch pharm Res.2004, 27(11):1183-1187.

Kim, S.H., Ahn,j. and Kwak H.S., 2004. Crosslinking of β_cyclodextrin on cholesterol removal from milk Archives of pharmacal research, 27(11):1183-1187.

Larsen, J. Froning, G. (1981). Extraction and processing of various components from egg youlk. Poultry science, 60: 160-167.

Lee, J., Ustunol, Z., and Smith, D. (1993). Cholesterol removal from cream using β-cyclodextrin and derivatives, Journal of Dairy Science, 76: 143.

Lee, D., Ahn, J., and Kwak, H. (1999). Cholesterol removal from homogenized milk with β-cyclodextrin, Journal of Dairy Science, 82: 2327–2330.

Makoto, K., Akio, O., and Reijiro, S. (1992). Cholesterol removal from animal with cyclodextrin by inclusion. Honnen LTD., assignee. Japan Pat. No. 4,168,198

Makiela, D., Janus, L., Gorny, K., Gburski, Z. (2018). Investigation Of The influence Of Beta cyclodextrin On Cholesterol Lodgement A molecular Dynamics Simulation Study, Journal of Molecular Liquids, 262 (2018) Pages 451-459.

Maskouki, A., Beheshti, H.R., Kheradmand, A., Valibeigi, S., Feizi, J. (2013). Investigating the Possibility of Cholesterol Reduction in Raw and Homogenized Milk By Using Beta-Cyclo dextrin, Journal of Research and Innovation in Food Science and Technology, 2 (3): Pages From 263-264.

Micich, T. (1990). Behaviors of polymer supported digitonin with cholesterol in the absence and presence of butter oil, Journal of Agricalture of Food Chemistery, 38: 1839–1843.

Noda, M., and Y. Shiinoki. (1986). Microstructure and rheological behavior of whipping cream. Journal of Texture Stud. 17:189–196.

Oakenfull, D., Sidhu, G., and Rooney, M. (1991). Cholesterol removal, Commonwealth Scientific and Industrial Research Organisation, assignee. International. Pat. No. WO 91/16824.

Oakenfull, D., and Sihdu, G. (1991). Cholesterol reduction, Commonwealth Scientific and Industrial Research Organisation, assignee. International. Pat. No. WO 91/11114.

O, Donnell, JA. (1989). Like fat technologies and marketing board (1989) Milk fat round tables. Journal of Dairy Science. 12:3109- 3115.

Oh, H., Chang, E., and Kwak, H. (1998). Conditions of the removal of cholesterol from milk by treatment with saponin, Korean Journal of Dairy Science. 20:253–260.

Pyorala, K. (1987). Dietary cholesterol in relation to plasma cholesterol and coronary heart disease, Am. J. Clin. Nutr. 45: 1176–1184.

Reineccius, T., Reineccius, G., and Peppard, T. (2004). Potential for β-cyclodextrin as partial fat replacer in low-fat foods, Journal of Food Science, 69: FCT334–FCT341.

Schroder, B., and Baer, R. (1990). Utilization of cholesterol reduced milk fat in fluid milks, Food Technology. (Nov.):145–148.

Seif hashemi, S., Tofangsazan, F. (2011). Technique of industrial production of cholesterol free butter. 20th National congress of Food Science and Technology, Sharif University of Technology, Iran.

Serajzadeh, S. (2005) . Cholesterol Reduction and Dairy Products by Enzymatic Method, Master,s Thesis, Faculty of Chemical and Petroleum Engineerng, Tehran, Sharif University.

Shim,S.Y., Ahn,J., and Kwak, S. Functional Properties of Cholesterol-Removed Whipping Cream Treated by β-cyclodextrin. J. Dairy Sci. 86: 2767-2772 American Dairy Science Association.(2003)

Smith, A., Goff, H., and Kakuda, Y. (2000). Microstructure and rheological properties of whipped cream as affected by heat treatment and addition of stabilizer, International Dairy Journal, 10: 295–301.

Szente, L., and Szejtli, J. (2004). Cyclodextrin as food ingredients. Trends Food Science Technol. 15: 137–142.

Vanderghem, C., Danthine, S., Blecker,C. and Deromanne, C. (2007), Effect of proteose addition on some physico- chemical characteristics of recombind dairy creams. International Dairy Journal. 17(8), 889- 895.

Vollbrecht, H. (1991). Process for the removal of cholesterol and cholesterol esters from egg yolk, SKW Trostberg Aktiengesellschaft, assignee, US Patent. No. 5063077.

Watanabe, K., Aihara, H. and Nakamura, R. (1989). Properties of the purified extracellular cholesterol oxidase from Rhodoccuse Equi. No. 23.Journal of Agriculture and Food Chemistry, 37: 1178-1182.

Yen, G., and Tsui, L. (1995). Cholesterol removal from a lardwater mixture with β-cyclodextrin, Journal of Food Science, 60:561–564.

Zahiraqdam, H.., Zandi, P . (2004). Investigating of Oil Cholesterol Reduction Rate during Treatment With Beta-Cyclodextrin, Summary Of 8th Iranian Nutrition Congress Articles, Iran University of Medical Sciences. Pages 386-387.

Zahiraqdam, H., Zandi, P.(2006). The Effects of Treatment Process With Betacyciodextrin on cholesterol in Cow Suet, Journal of Nutrition Science and Food Industry of Iran, Issue 1 , Pages From 1-6.