Comparison of technological characteristics of native starter isolates from traditional Khorasan yogurts with commercial starter in yogurt production

Document Type : Full Research Paper

Authors

• Fatemeh Barmak ¹
• Mohammad Bagher Habibi Najafi ¹
• Reza Hajimohammadi Farimani ²
• Mohammad Reza Edalatian Dovom ¹

¹ Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

² Department of Food Science & Technology, Faculty of Agriculture, Shahid Bahonar University of Kerman

https://doi.org/10.22067/ifstrj.v17i4.86655

Abstract

Introduction: One of the most important factors in producing yogurt is choosing the right starter. Native isolates of any country are among the genetic resources of that country, which play a major role in the production and development of the organoleptic properties of fermented products. Therefore, it seems necessary to study the industrial applications of native isolates. In this study, the production of yogurt was investigated by using native starter isolates from traditional Khorasan yogurts and its comparison with yogurts produced with two types of commercial starters.
 
Materials and methods: Six strains of Streptococcus thermophilus and three strains of Lactobacillus delbrueckii subsp. Bulgaricus isolated from traditional Khorasan yogurts were selected. The proteolytic activity of Streptococcus thermophilus strains was determined according to the method of Erkus et al(2012) and the proteolytic activity of Lactobacillus delbrueckii subsp. Bulgaricus was determined according to the method of Nespolo et al.(2010). Milk acidification activity byall examined strains was evaluated according to the method of Erkus et al.(2012). The production property of gamma-aminobutyric acid of native isolates was also evaluated using the method of Lacroix et al. (2013). Yogurt was produced using commercial starters and native isolates. Acidity and pH were measured according to Iranian National Standard No. 2852. Synersis of yogurt samples was measured using centrifugation according to Çelik (2007) method. In order to measure textural properties, a combination of backward extrusion techniques and Texture Profile Analysis (TPA) was used (Yang et al, 2010). Sensory characteristics of yogurt samples were evaluated by 20 panelists using five-point hedonic scale. This study was conducted based on factorial experiment using a completely randomized design. Statistical analysis of data was performed using Minitab Statistical Software (version 17) and ANOVA. Comparison of means was performed using Tukey’s test at the significant level (p < 0.05).
 
Results and discussion: The results of proteolytic activity of Lactobacillus delbrueckii subsp. Bulgaricus showed that the L₃ code was weaker than the other two strains. All Streptococcus thermophilus strains were positive protease. All Streptococcus thermophilus strains of the study decreased the pH of the milk to 4.6 in less than five hours, and Streptococcus thermophilus strain S₆ code, as the fastest acid producer, decreased the pH of the milk to 4.6within 3 and half hours (Figure 2). The results of GABA production showed that among the streptococcus thermophilus strains, S₁, S₅ and S6 codes had distinct blue discoloration. Among the Lactobacillus strains, except the L₃ strain, two other strains produced green color. Of the 18 samples of the yoghurt, S₅L₂ and S₂L₂ samples showed the highest decrease in pH and increased in acidity compared to other samples after being stored refrigerated overnight (P <0.05). The other samples had the same pH and acidity changes as the control samples after overnight refrigeration. The results show the ability of yogurt producion by native starters to compete with those produced by commercial starters in terms of technological characteristics. Thus, samples with codes S₂L₂, S₃L₁, S₃L₂, and S₁L₁ showed less Synersis of   yogurt than commercial starters. This decrease in the Synersis of yogurt might due to the possibility of exopolysaccharide production by these strains. Textural characteristics of yogurt samples were the hardness of the samples in the range of 3 to 4 N, which was significantly different (P <0.05). The lowest hardness was observed for the S₃L₃, which can be attributed to the poor proteolytic activity of the strain. L₃, S₆L₂, S₃L₂ and S₂L₂ had the highest   hardness compared to other compounds and control samples (P <0.05). This differences in the hardness of the samples can be attributed to the possible production of some metabolites by the strains. There was no statistically significant difference in the adhesion properties of the samples (p <0.05). Considering the sensory evaluation scores, it was found that in all respects, S₃L₃ had the lowest score among the other samples (P <0.05). This was in good correlation with the results of the textural properties of the test. Therefore, this compound was eliminated compared to the control samples. Finally, considering the proteolytic behavior of the strains and the ability to produce acid and GABA, as well as the sensory, rheological and physicochemical properties of 18 samples in comparison to the two control samples, Streptococcus thermophilus strains with codes S₄, S₂, S₅, S₁, S₆, and Lactobacillus delbrueckii subsp. Bulgaricus strains with codes L₁ and L₂ have the potential to be used as commercial starters in the form of compounds S₁L₁, S₂L₂, S₅L₁, S₆L₂, S₄L₂.

 

Keywords

• Synersis
• Acidity
• Native starter
• Yogurt
• Texture profile

Main Subjects

• Food Biotechnology

References

 

Amani, E., Eskandari, M H., Shekar Forosh, S., Hanif Pour, M A.2015. Investigation the effects of proteolytic activity and exopolysaccharide production by native starter bacteria on technological and organoleptic properties of yogurts. Food Science and Technology. 13 (58):15-29.

Birollo, G.A., Reinheimer, J.A., and Vinderola, C.G. 2000. Viability of lactic acid microflora in different types of yoghurt. Journal of Food Research International, 33: 799-805.

Bourne M.C. 2002. Food Texture and Viscosity: Concept and Measurement. Academic press. London.

Burkus, Z., and Temelli, F. 2005. Rheological properties of barley b-glucan. Journal of Carbohydrate Polymers, 59: 459-465.

BumPark, k., & Oh, S.H. 2007. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresource Technology, 98,1675-1679.

Çelik, E.F. 2007. Determination of aroma compounds and exopolysaccharide formation by Lactic acid bacteria isolated from traditional yoghurt. MSc Thesis, Izmir institute of technology.

Chieh Wang, Y., Chui Yu, R. and Chun, C.C. 2002. Growth and survival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk drinks. Journal of Food Microbiology, 19: 501-508.

Corrieu, G., and Béal, C. 2016. Yogurt: The Product and its manufacture. Encyclopedia of Food and Health, 617-624.

Cruz, N.S., Capellas, M., Jaramillo, D.P., Trujillo, A.J., Guamis, B., and Ferragut, V. 2009 .Soymilk treated by ultra-high pressure homogenization: acid coagulation properties and characteristics of a soy-yoghurt product. Journal of Food Hydrocolloids, 23: 490-496.

Dave, R., and Shah, N. 1997. Effect of level of starter culture on viability of yoghurt and probiotic bacteria in yoghurts. Food Australia Journal, 49: 164-168.

De Vuyst, L. 2000. Technology aspects related to the application of functional starter cultures, Food Technology and Biotechnology, 38(2): 105-113.

Dincel, S. 2012. Chemical and rheological properties of yoghurt produced by lactic acid cultures isolated from traditional Turkish yoghurt. A thesis submitted to the graduate school of natural and applied sciences of Middle East Technical University, Turkey.

Domagala, J. 2008. Sensory evaluation and rheological properties of yoghurts prepared from goat, cow and sheep milk. Electronic Journal of Polish Agricultural Universities, 11 (3): art. 4.

Donohue, D.C. 2004. Safety of Novel Probiotic Bacteria. In: Lactic Acid Bacteria–Microbiological and functional aspects, 3rd edition. Salminen, S., von Wright, A.,Ouwehand, A. Marcel Dekker, Inc. NY, USA.

Ebdali, S., and Motamedzadegan, A. 2013. The effect of replacement for part of dry material with gelatin to functional properties of fat-free’s set yogurt. Journal of Food Industry, 2: 221-229. (In Farsi).

Erkus, O., Okuklu, B., Yenidunya, A.F., and Harsa, S. 2014. High genetic and phenotypic variability of Streptococcus thermophilus strains isolated from artisanal Yuruk yoghurts. LWT - Food Science and Technology, 58: 348-354.

Eskandari, m., roshanzadeh, s., jafarpour, d., shokrforosh, sh.1390. Investigation of important industrial technological features of isolated starter bacteria from native Iranian yogurts. Twentieth national congress of food science and technology.

Fadela, C., Abderrahim, C., and Ahmed, B. Sensorial and Physico-Chemical Characteristics of Yoghurt Manufactured with Ewe's and Skim Milk. 2009.  Journal of Dairy & Food Sciences, 4(2): 136-140.

Franciosi, E., Settanni, L., Cavazza, A., and Poznanski, E.2009. Biodiversity and technological potential of wild lactic acid bacteria from raw cows’ milk. International Dairy Journal, 9: 3-11.

Forgani, S., Peighambardoust, S. H., Hesari, J., Rezai Mokarram, R.2018. Effect of adding millet milk on viability of Lactobacillus acidophilus LA-5, starter bacteria and some physicochemical characteristics of the probiotic yogurt. Journal of Food Science & Technology. 76(15): 207-219.

Gauche, G., Tomazi, T., Barreto, P.L.M., Ogliari, P.G., and Bordignon-Luiz, M.T. 2009. Physical properties of yoghurt manufactured with milk whey and transglutaminase. LWT - Food Science and Technology, 42(1): 239-243.

Ghorbani Hasansaraei, A. 1393. Yogurt fortification with different sources of omega3 and evaluation of its physicochemical and Sensory properties during storage. PhD Thesis, Ferdowsi University of Mashhad.

Güler-Akın, M.B., and Akın, S.M. 2007. Effects of cysteine and different incubation temperatures on the microflora, chemical composition and sensory characteristics of bio-yogurt made from goat’s milk. Journal of Food Chemistry, 100: 788-793.

Guggisberg, D., Cuthbert-Steven, J., Piccinali, P., and Bütikofer, U.P. 2009. Rheological, microstructural and sensory characterization of low-fat and whole milk set yoghurt as influenced by inulin addition. International Dairy Journal, 19: 107-115.

Habibi Najafi , M.B.,  Razavi, M.A.,  Mazaheri Tehrani, M.1377. Yogurt knowledge and technology: our knowledge (Vol. 1). Richard Kenneth Robinson , Tamim . Jihad Daneshgahi (Ferdowsi University of Mashhad).

Hajimohammadi Farimani, R., Habibi Najafi, M.B., Fazly Bazzaz, B.S., Edalatian, M.R. Bahrami, A.R., Belén Flórez, A., Mayo, B. 2016. Identification, typing and functional characterization of dominant lactic acid bacteria strains from Iranian traditional yoghurt. Journal of Eur Food Res Technol, 242: 517-526.

Hajimohammadi Farimani, R. 1394. Isolation, phenotypic and genotypic characterization of lactic acid bacteria from artisanal yoghurts in khorasan and study the technological properties of yoghurt Producing strains. PhD Thesis, Ferdowsi University of Mashhad.

Hagiwara, H., Seki, T., & Ariga, T. 2004. The Effect of Pre-germinated Brown Rice Intake on Blood Glucose and PAI-1 Levels in Streptozotocin induced Diabetic Rats. Bioscience, Biotechnology, and Biochemistry, 68(2), 444-447.

Hanh, T.H.N., Ong, L., Kentish, S.E., and Gras, S.L. 2015. Homogenisation improves the microstructure, syneresis and rheological properties of buffalo yoghurt. International Dairy Journal, 46: 78-87.

Han, X., Zhang, L., Yu, P., Yi, H., and Zhang, Y. 2014. Potential of LAB starter culture isolated from Chinese traditional fermented foods for yoghurt production. International Dairy Journal, 34: 247-251.

Huang, J., Mei, L., Sheng, Q., Yao, S., & Lin, D. 2007. Purification and Characterization of glutamate decarboxylase of Lactobacillus brevis CGMCC 1306 isolated from fresh milk. Chemical Engineering. 15(2), 157-61.

Innocente, N., Biasutti, M., Rita, F., Brichese, R., Comi, G., and Iacumin, L. 2016. Effect of indigenous Lactobacillus rhamnosus isolated from bovine milk on microbiological characteristics and aromatic profile of traditional yogurt. Journal of Food Science and Technology, 66: 158-164.

Isanga, J., and Zhang, G. 2009. Production and evaluation of some physicochemical parameters of peanut milk yoghurt. Journal of Food Science and Technology, 42(6): 11321138.

Isleten, M., and Karagul-Yuceer, Y. 2006. Effects of dried dairy ingredients on physical and sensory properties of non fat yogurt. Journal of Dairy Science, 89(8): 2865-2872.

Kailasapathy, K. 2006. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. Journal of Food Science and Technology, 39(10):1221-1227.

Karazhyan, H., Salari, R.2011. Comparison of physic-chemical, rheological and sensory properties of yoghurt made from fresh cow’s milk and powdered milk. Journal of Food Science and Technology, 2(8): 11-19.

  Lacroix, N., St-Gelais, D., Champagne, C.P., and Vuillemard, J.C. 2013. Gamma-aminobutyric acid-producing abilities of lactococcal strains isolated from old-style cheese starters. Journal of Dairy science and Technology, 93: 315–327.

Lee, W., and Lucey, J. 2010. Formation and physical properties of yogurt. Asian-Aust. Journal of Animal Science, 23: 1127-1136.

Letort, C., Nardi, M., Garault, P., Monnet, V., and Juillard, V. 2002. Casein utilization by Streptococcus thermophilus results in a diauxic growth in milk. Journal of Applied and Environmental Microbiology, 68: 3162-3165.

Li, H., & Cao, Y. 2010. Lactic acid bacterial cell factories for gamma-aminobutyric acid Amino Acids, 39, 1107-1116.

Lucey, J.A. 2001. The relationship between rheological parameters and whey separation in milk gels. Journal of Food Hydrocolloids, 15: 603-608.

Lucey, J.A. 2002. Formation and physical properties of milk protein gels. Journal of Dairy Science, 85(2): 281-294.

Marshall, V.M., and Rawson, H.L. 1999. Effects of exopolysaccharide producing strains of thermophilic lactic acid bacteria on the texture of stirred yogurt. International Journal of Food Science and Technology, 34: 137-143.

Nespolo, C.R. and Brandelli, A. 2010. Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese. Brazilian Journal of Microbiology, 41: 1009-1018.

Ng, E W., Yeung, M., and Tong, P S. 2011. Effects of yogurt starter cultures on the survival of Lactobacillus acidophilus. International Journal of Food Microbiology, 145(1): 169-175.

Oliveira, M.N., Sodini, I., Remeuf, F. and Corrieu, G. 2001. Effect of milk supplementation and culture composition on acidification, textural properties and Microbiological stability of fermented milks containing probiotic bacteria. Journal of International Dairy, 11: 935-942.

Pimentel, T.C., Garcia, S., and Prudencio, S.H. 2012. Effect of long-chain inulin on the texture profile and survival of Lactobacillus paracasei ssp. paracasei in set yoghurts during refrigerated storage. International Journal of Dairy Technology, 65(1):104-110.

Pourahmad, R., and Mazaheriassadi, M. 2005.Yoghurt production by Iranian native starter cultures Nutrition & Food Science, 35(6): 410-415.

pourahmad, R., and Mazaheriassadi, M. 2010. Evaluation of acetaldehyde content in yogurts produced by native microbial strains. Journal of Food Technology and Nutrition. 7 (26); 2-9.

Qiyamati Yazdi, F., 1391. Population diversity of lactic acid bacteria in south Khorasan, Iran. Thesis, Gorgan University of Agricultural Sciences and Natural Resources - Faculty of Agriculture.

Ramchandran, L., and Shah, N.P. 2009. Effect of exopolysaccharides on the proteolytic and angiotensin-I converting enzyme-inhibitory activities and textural and rheological properties of low-fat yogurt during refrigerated storage. Journal of Dairy Science, 92(3): 895-906.

Ramchandran, L., and Shah, N.P. 2010. Characterization of functional, biochemical and textural properties of symbiotic low-fat yogurts during refrigerated storage. Journal of Food Science and technology, 43: 819-827.

Rajiv, I., and Negendra, P. Sh. 1997. Viability of Yoghurt and Probiotic Bacteria in Yoghurts Made from Commercial Starter Cultures. International Dairy Journal, 7: 31-41.

Robinson, R.K. 2007. Manufacture yoghurt and fermented milk. International Dairy Journal. 60(3): 237-247.

Robinson, R.K. 2005. Dairy Microbiology Handbook: The Microbiology of Milk and Milk Products. John Wiley & Sons.

Sanders, JW, Leenhouts, K., Burghoorn, J., Brands, JR., Venema, G., & Kok, J.A. 1998. Chloride-inducible acid resistance mechanism in Lactococcus lactis and its regulation. Molecular Microbiology, 27(2), 299-310.

Serra, M., Trujillo, A.J., Guamis, B., and Ferragut, V. 2009. Evaluation of physical properties during storage of set and stirred yogurts made from ultra-high pressure homogenization-treated milk. Journal of Food Hydrocolloids, 23(1): 82-91.

Shaker, R.R., Jumah, R.Y., and Abu-Jdayil, B. 2000. Reological properties of plain yoghurt during coagulation process: impact of fat content and preheat treatment of milk. Journal of Food Engineering, 44: 175-180.

Shoji, A.S., Oliveira, A.C., Balieiro, J.C.C., Freittas. O., Thomazini, M., Heinemann, R.J.B., Okuro, P.K., and Favaro, C.S. 2013. Viability of L.acidophilus microcapsules and their application to buffalo milk yoghurt. Journal of Food and Bioproducts Processing, 91: 83-88.

Soomro, A. H., and Masud, T. 2008. Selection of yoghurt starter culture from indigenous isolates of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus on the basis of technological properties. Journal of Annals of Microbiology, 58 (1): 67-71.

Sybesma, W., Hugenholtz, J., de Vos, W.M. Smid, E.J. 2006. Safe use of genetically modified lactic acid bacteria in food. Bringing the gap between consumers, green groups, and industry. Electronic journal of Biotechnology, 9(4): 424-448.

Tamime, A.Y., Robinson, R.K. 2007. Yoghurt: Science and Technology. 3rd ed. Woodhead Publishing. Cambridge, UK.

Ueno, H. 2000. Enzymatic and structural aspects on glutamate decarboxylase. Molecular Catalysis B: Enzymatic, 10, 67-79.

Xanthopoulos, V., Petridis, D., Tzanetakis, N. 2001. Characterization and classification of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus strains isolated from traditional Greek yoghurts. Journal of Food Science. 66:747-752.

Yang, M., and Li, L. 2010. Characteristics of Probiotic Soy Yogurt, Journal of Food technology Biotechnology, 48(4): 490-496.

 Yvon, M. 2006. Key enzymes for flavour formation by lactic acid bacteria. Australian Journal of Dairy Technology, 61: 80-96.

Zhao, Q.Z., Wang, J.S., Zhao, M.M., Jiang, Y.M., and Chun, C. 2006. Effect of Casein Hydrolysates on Yogurt Fermentation and Texture Properties during Storage. Journal of Food Technology and Biotechnology. 44 (3): 429–434