محاسبه عددی کشندگی باکتری در شیر بطری شده تحت تیمار پلاسمای سرد

نوع مقاله : مقاله پژوهشی لاتین

نویسنده

• آزاده رنجبر ندامانی

دانشگاه علوم کشاورزی و منابع طبیعی ساری

https://doi.org/10.22067/ifstrj.2023.81316.1238

چکیده

در سال‌های اخیر، پلاسمای سرد یکی از جایگزین‌های مورد انتظار برای تیمارهای پس از برداشت محصولات هستند. در این مطالعه، یک سیستم تخلیه سطحی برای جستجوی زمان نابودی باسیلوس سرئوس، باسیوس کوآگولانس، باسیلوس اسئاروترموفیلوس و کلستریدیوم بوتولینیوم در شیر بطری شده استفاده شد. شبیه‌سازی توسط نرم‌افزار COMSOL ورژن a5/3 برای یک هندسه دو بعدی اجرا شد. داده‌های آزمایشی جمع‌آوری شده در نرم‌افزار شبیه‌سازی شدند. فاکتور k  حاصل از داده‌های غیرفعال‌سازی میکروارگانیسم برای تأیید داده‌های شبیه‌سازی استفاده شد. نتایج نشان دادند تولید گونه‌های فعال اکسیژن طی تیمار پلاسمای سرد، با افزایش زمان افزایش می‌یابد و در کل ظرف پخش می‌شود. غلظت این گونه‌ها در ابتدای تولید یعنی در لحظه خروج از پروب پلاسما، بالا بوده و در انتها که سطح آزاد شیر را ترک می‌کنند، کاهش می‌یابد. با افزایش دمای اولیه نمونه شیر از 50 به 80 درجه سانتی‌گراد، می‌توان تغییرات بارزی در مقدار ازون مشاهده کرد. اما تغییرات ولتاژ در این دو دما اثر بارزی بر غلظت ازون نداشت. همچنین بلافاصله با آغاز تیمار پلاسما، تولید پلاسما نیز آغاز شده و میزان غلظت گونه‌های فعال در آن لحظه بیشترین مقدار است. نشان داده شده است که در بین چهار باکتری مورد مطالعه، باسیلوس استئاروترموفیلوس بیشترین مقاومت را در برابر پلاسمای سرد داشته و باکتری‌های دیگر بعد از آن قرار می‌گیرند. در نهایت می‌توان نتیجه گرفت که تیمار پلاسما در عمق بطری، این امکان را ایجاد می‌کند که محدودیت کاربرد سطحی تیمار پلاسمای سرد رفع شود.

کلیدواژه‌ها

• پلاسمای سرد
• شیر
• استریلیزاسیون
• پاستوریزاسیون

موضوعات

• فناوری مواد غذایی

عنوان مقاله [English]

Numerical calculation of the lethality of becteria in bottled milk under cold plasma treatment

نویسنده [English]

• Azadeh Ranjbar Nedamani

Sari Agricultural Sciences and Natural Resources University

چکیده [English]

In recent years, cold plasma is one of the expected alternatives for post-harvest treatments and post-harvest management of products. A surface discharge plasma system was used for investigating the destruction time of Bacillus cereus, Bacillus coagulans, Bacillus stearothermophilus, and Clostridium botulinum in bottled milk. The simulation was performed by COMSOL a3.5 software for a two-dimensional geometry. The collected experimental data were simulated in COMSOL software. The k factor of microorganism deactivation data was used to validate the simulated data. Results showed that the production of reactive oxygen species during plasma treatment increases with time and extends to the entire container. The concentration of reactive oxygen species (at the output of the plasma probe) at the beginning of the production was high, and at the end when they leave the free surface of the milk, the concentration decreased. Increasing the initial temperature of milk sample, from 50 to 80℃, can cause significant changes in the amount of ozone from 125 mol/m³ to 266 mol/m³, respectively (p <0.05). However, voltage changes in these two temperatures did not show a significant effect on ozone concentration. Also, immediately upon the initiation of plasma treatment, plasma destruction begins where the concentration of active species is higher. It is shown that among the four studied bacteria, Bacillus stearothermophilus has the highest resistance against cold plasma, and after that other bacteria have shown similar resistance. Finally, it can be concluded that the deep plasma treatment in bottle can make it possible to overcome the surface limitation of cold plasma treatment.

کلیدواژه‌ها [English]

• Cold plasma
• Milk
• Sterilization
• Pasteurization

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