Influence of simulated gastrointestinal conditions on survivability of microencapsulated probiotic Lactobacillus casei in symbiotic frozen yogurt

Document Type : Research Article

Authors

• Elnaz Milani ¹
• Hajar Naeemi
• Seyed Ali Mortazavi ²
• Arash Koocheki ³

¹ ACECR; Khorasan Razavi

² Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran.

³ Ferdowsi University of Mashhad

https://doi.org/10.22067/ifstrj.v8i2.17279

Abstract

In this study, symbiotic yog-ice (rozen yogurt) was produced that was inoculated with different levels of inulin(0,2.5 amd 5%)as prebiotic compound and Lactobacillus Casei as probiotic bacteria in two types; free and encapsulated with Sodium Algint-Whey Protein Concentration for low fat yog-ice cream.The survivability of this probiotic bacteria was evaluated under simulated gastro-intestinal conditions ,during 30 days storage at -18oC. In the samples with free state of probiotic bacteria, the number of viable cells ,after 1h exposuring simulated gastric juice(pH=2,pepcine) decreased from 9.78-9.81 log cfu/ml to 6.97-7.26 log cfu/ml,at the end of 30 days storage at -18oC,while, the viable number of encapsulated state of probiotic bacteria, after 1h exposuring simulated gastric juice, decreased from 8.15-8.66 log cfu/ml to 6.80-7.09 log cfu/ml at the end of 30 days storage at -18oc.After exposuring simulated gastric juice, samples was ezposured 2.5h under simulated intestinal juice(pH=7.4,panceratin), and the results that was gained showed that, the samples containing free probiotic bacteria after 2.5h exposuring simulated intestinal juice decreased from 8.808-9.355 log cfu/ml to 5.159-5.9 log cfu/ml at the end of 30 days storage at -18oC.While, in the samples containing sodium alginate-whey protein concentration capsules, the viable number of cells decreased from 7.38-8.201 log cfu/ml to 6.151-6.672 log cfu/ml, at the end of 30 days storage at -18oC. The results indicate that there are significant differences (p

Keywords

• Sodium Alginat-Whey Protein Concentration encapsulation
• Inulin
• L. Casei
• simulated gastrointestinal conditions
• SEM electron microscope

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