با همکاری انجمن علوم و صنایع غذایی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشگاه تربیت مدرس

چکیده

در این تحقیق پروتئین آبکافت از ماهی ساردین پهلو طلایی (Sardinella gibossa) با استفاده از آنزیم پاپائین تولید گردید و شرایط آبکافت (زمان, دما و فعالیت آنزیم) جهت حصول به خاصیت آنتی اکسیدانی مطلوب با استفاده از روش سطح پاسخ بهینه سازی شد. ضریب تعیین کلی، ضریب تعیین اصلاح شده و ضریب دقت محاسبه شده توانایی تخمین مدل را نشان داد. شرایط بهینه این آزمایش عبارت بود از غلظت آنزیم 2% , زمان30 دقیقه و دمای 45 درجه سانتی گراد که در نتیجه بر اساس چنین شرایطی میزان حذف رادیکال آزاد DPPH برابر 5/64 درصد بدست آمد. بر اساس نتایج آنالیز اسید آمینه و شاخص شیمیایی متیونین و ایزولوسین اسیدهای آمینه محدود کننده بودند اما دیگر اسیدهای آمینه به میزان لازم وجود داشتند. در نتیجه گیری نهایی می‌توان گفت که مدل حاصل از شرایط خوبی جهت پیش بینی برخوردار است و پروتئین آبکافت ماهی ساردین پهلو طلایی دارای خواص آنتی اکسیدانی مطلوبی در سطح بهینه می‌باشد و می‌توان از این فرآورده شیلاتی جهت استفاده به عنوان مکمل غذایی استفاده نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Antioxidant Activity Optimization of Gold stripe Sardine (Sardinella gibossa) Protein Hydro-lysate by RSM

نویسندگان [English]

  • Ali Taheri
  • Abdolmohammad Abedian Kenari
  • Ali Motamedzadegan
  • Mehran Habibi Rezaie

Tarbiat Modares University, Faculty of Natural Resource and Marine Science

چکیده [English]

Protein hydro-lysate was prepared from Gold striped sardine (Sardinella gibossa) by Papain. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design and anti-oxidative activity was the dependent variable. Results of the total determination coefficient, adjusted R2 and predicted R2 indicating the validity of prediction model and the experiment error was very small. An enzyme activity to substrate ratio (X1), 2%; time (X2), 30 minute, and temperature (X3), 45 °C were found to be the optimum conditions for a higher DPPH free radical scavenging activity (64.5%), using Papain. According to amino acid analysis results and chemical score, methionine and Isoleucine were the limiting amino acids in the hydro-lysate. The protein hydro-lysate tends to be a functional additive in food industries.

کلیدواژه‌ها [English]

  • Protein hydro-lysate
  • Anti-oxidative activity
  • Gold striped sardine
  • Enzyme
  • Methionine
  • Chemical score
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