تهیه سامانه امولسیون  ژل سرد با استفاده از پیکرینگ های کمپلکس ایزوله پروتئین سویا-صمغ دانه ریحان به‌عنوان جایگزین چربی در خامه

نوع مقاله : مقاله پژوهشی

نویسندگان

• سیما ناجی طبسی ¹
• الهام مهدیان ¹
• اکرم آریان فر ¹
• سارا ناجی طبسی ²

¹ گروه علوم و صنایع غذایی، واحد قوچان، دانشگاه آزاد اسلامی، قوچان، ایران

² گروه نانوفناورى مواد غذایى، موسسه پژوهشی علوم و صنایع غذایی، مشهد، ایران

https://doi.org/10.22067/ifstrj.2020.39273

چکیده

مصرف زیاد چربی خطر ابتلا به بیمار‌ی‌های مختلفی را افزایش می دهد، اما به‌دلیل نقش چندگانه چربی در فرآورده‌های غذایی، حذف یا کاهش آن منجر به ایجاد خصوصیات کیفی نامطلوب می‌گردد. بنابراین، در این تحقیق استفاده از ساختار امولسیون‌ژل سرد پایدارشده با استفاده از پیکرینگ های ایزوله پروتئین سویا (ISP) و کمپلکس ایزوله پروتئین سویا-صمغ دانه ریحان (ISP-BSG) به‌عنوان جایگزین چربی موردمطالعه قرارگرفت. ذرات پیکرینگ ISP-BSG به‌‎ترتیب با نسبت‌های مختلف جرمی ISP:BSG، 0:1، 1:1، 1:2 و 1:3 تهیه و به‌ترتیب 1S:0B ،1S:1B ،2S:1B و 3S:1B نام‌گذاری شدند. بررسی ویژگی های امولسیون ژل نشان داد که پوشش دهی سطح قطرات امولسیون توسط نانوذرات جامد S:B یک پوسته سفت و سخت را تشکیل می‌دهد. با افزودن کلسیم کلرید به امولسیون، شبکه‌های امولسیون‌ ژل از قطرات روغن تجمع‌یافته تشکیل‌ شدند که این ساختار در نمونه‌های 1S:0B و 2S:1B قابل‌مشاهده‌تر بود. همچنین، نوع و ساختار پیکرینگ به‌کار‌رفته بر مقدار حفظ روغن در امولسیون ژل اثرگذار بود. حضور BSG در نسبت مناسب (2S:1B) با ISP باعث ایجاد امولسیون ژل باثبات‌تر با استحکام مکانیکی (سختی و قوام) بالاتری شد. با توجه به پایداری و خصوصیات بافتی از بین نمونه‌های امولسیون ژل، دو فرمول 1S:0B و 2S:1B برای تولید خامه با چربی کاهش‌یافته (5، 10 و 15%) استفاده شد. استفاده از درصدهای بیشتر از 5 درصد جایگزینی با افزایش آب‌اندازی خامه همراه بود (05/0p <)، اما نوع امولسیون‌ ژل بر میزان آب‌اندازی تأثیر معنی‌داری نداشت. بیشترین میزان پذیرش کلی با جایگزینی امولسیون ژل 2S:1B با کاهش 5% چربی حاصل شد (05/0p>).

کلیدواژه‌ها

• امولسیون  ژل
• ایزوله پروتئین سویا
• ذرات جامد
• صمغ دانه ریحان
• کمپلکس
• جایگزینی چربی

موضوعات

• فناوری مواد غذایی

عنوان مقاله [English]

Preparation of cold gel emulsion system using isolated soy protein- Basil seed gum complex as a fat replacement in cream

نویسندگان [English]

• Sima Naji-Tabasi ¹
• Elham Mahdian ¹
• Akram Arianfar ¹
• Sara Naji-Tabasi ²

¹ Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran.

² Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST) PO Box, 91895-157.356, Mashhad, Iran.

چکیده [English]

Introduction: Fats have a special place in human nutrition and their main role is supplying energy for the body. But scientific findings approve an association between high fat intake and an increment risk of some diseases, such as atherosclerosis, heart disease, and …. Demand for low-fat foods has increased dramatically as people become more aware of fat consumption side effects. However, due to the multiple role of fats in food products, eliminating or decreasing fat lead to poor quality of products. Therefore, it is necessary to use a combination of fat substitutes to maintain the rheological, texture and sensory properties of the low fat food products. The use of emulsion gel structures are new methods for the production of low-fat product that has been studied in this investigation. The Pickering emulsion was used to produce the emulsion. In this method, instead of using surface-active agents, solid particles are used to stabilize the emulsion. The aim of this study was to prepare Pickering Emulsion from Isolated Soybean protein (ISP) and Basil Seed Gum (ISP-BSG) Complex. Finally, emulsion gel systems were applied as fat substitutes in cream.
 
Materials and Methods: Basil seeds were purchased from Mashhad market. Isolated soy protein was purchased from Shandong Yuxin Bio-Tech Co. (China). Sodium azide was purchased from Applichem Inc. (Dramstadt, Germany). Sodium dodecyl sulphate (SDS) was obtained from Merck, Germany.  Pickering (solid particles) of soy protein isolate (SPI) and SPI-basil seed gum (ISP-BSG) complex used as emulsifier for stabilization of cold emulsion. ISP-BSG particles were prepared with different mass ratios of ISP: BSG, 1: 0, 1: 1, 2: 1 and 3: 1 and named 1S: 0B, 1S: 1B, 2S: 1B and 3S: 1B, respectively. These solid particles were used as Pickering for emulsion preparation. Cold Emulsion was prepared by adding calcium chloride. The oil leakage, oil leakage after thermal treatments, microscopic structure, textural properties and viscoelastic properties of emulsion gel were studied. Then, the best structures used as a fat substitute in cream (5, 10 and 15%).
 
Results & Discussion: Investigation of emulsion gel properties showed that coating the surface of emulsion droplets with solid nanoparticles formed a rigid shell that acted as a barrier against the deformation and transfer of materials from the interfacial surface. The use of ISP-BSG nanoparticles as picking particles caused more homogeneity and stiffness in emulsion gel structure. The rate of water loss in reduced fat cream was in the range of 1-2% by using the emulsion-gel system, which indicates the effectiveness of these systems in reducing dehydration in cream. The increasing replacement percentages led to increase water loss (p <0.05). However, emulsion type had no significant effect on water loss content (p> 0.05). According to the results, ISP-BSG nanoparticles impart high potential to stabilize emulsion with small oil droplets. Based on the results of the sensory test, it was found that the characteristics of the cream samples depended more on the replacement rate than the type of system used as an alternative. The presence of a fat replacement system maintained the desired quality in low-fat cream samples. Most of the samples scored higher than 3, which indicate the high acceptance of low-fat samples.

کلیدواژه‌ها [English]

• Basil seed gum
• Complex
• Emulsion gel
• Fat replacement
• Soy protein isolate
• Solid particles

مراجع

Alting, A,. C. (2003). Cold gelation of globular proteins. Thesis Wageningen University, The Netherlands.

Arditty, S., V. Schmitt, J. Giermanska-Kahn and F. Leal-Calderon (2004). "Materials based on solid-stabilized emulsions." Journal of Colloid and Interface Science 275(2): 659-664.

Arkoumanis, P. G., I. T. Norton and F. Spyropoulos (2019). "Pickering particle and emulsifier co-stabilised emulsions produced via rotating membrane emulsification." Colloids and Surfaces A: Physicochemical and Engineering Aspects 568: 481-492.

Ayed, C., S. I. Martins, A. M. Williamson and E. Guichard (2018). "Understanding fat, proteins and saliva impact on aroma release from flavoured ice creams." Food chemistry 267: 132-139.

Brodelius, P. E., C. Funk and R. D. Shillito (1988). "Permeabilization of cultivated plant cells by electroporation for release of intracellularly stored secondary products." Plant cell reports 7(3): 186-188.

Clark, A. H. and S. B. Ross-Murphy (1987). Structural and mechanical properties of biopolymer gels. Biopolymers, Springer: 57-192.

Costa, C., Medronho, B., Filipe, A., Mira, I., Lindman, B., Edlund, H., & Norgren, M. (2019). Emulsion formation and stabilization by biomolecules: The leading role of cellulose. Polymers, 11(10), 1570.

De Vries, A., J. Hendriks, E. Van Der Linden and E. Scholten (2015). "Protein oleogels from protein hydrogels via a stepwise solvent exchange route." Langmuir 31(51): 13850-13859.

Fiszman, S. and M. Damasio (2000). "Suitability of single‐compression and TPA tests to determine adhesiveness in solid and semi‐solid foods." Journal of texture studies 31(1): 55-68.

Ge, S., L. Xiong, M. Li, J. Liu, J. Yang, R. Chang, C. Liang and Q. Sun (2017). "Characterizations of Pickering emulsions stabilized by starch nanoparticles: Influence of starch variety and particle size." Food chemistry 234: 339-347.

Hesarinejad, M. A., S. M. Razavi and A. Koocheki (2015). "Alyssum homolocarpum seed gum: Dilute solution and some physicochemical properties." International journal of biological macromolecules 81: 418-426.

Hoefler, A. (2001). Carrageenan: chemistry, functionality, and applications, Oxford PA. p.

Jo, C. and D. U. Ahn (1999). "Fat reduces volatiles production in oil emulsion system analyzed by purge and trap dynamic headspace/gas chromatography." Journal of Food Science and Technology (Mysore) 64.4: 641-643.

Katsiari, M., L. Voutsinas, E. Kondyli and E. Alichanidis (2002). "Flavour enhancement of low-fat Feta-type cheese using a commercial adjunct culture." Food Chemistry 79(2): 193-198.

Kavas, G., G. Oysun, O. Kinik and H. Uysal (2004). "Effect of some fat replacers on chemical, physical and sensory attributes of low-fat white pickled cheese." Food chemistry 88(3): 381-388.

Khouryieh, H., G. Puli, K. Williams and F. Aramouni (2015). "Effects of xanthan–locust bean gum mixtures on the physicochemical properties and oxidative stability of whey protein stabilised oil-in-water emulsions." Food chemistry 167: 340-348.

Kuhn, K. R., Â. L. F. Cavallieri and R. L. Da Cunha (2010). "Cold‐set whey protein gels induced by calcium or sodium salt addition." International journal of food science & technology 45(2): 348-357.

Lee, M. N., H. K. Chan and A. Mohraz (2011). "Characteristics of pickering emulsion gels formed by droplet bridging." Langmuir 28(6): 3085-3091.

Li, C., Y. Li, P. Sun and C. Yang ,2013). Pickering emulsions stabilized by native starch granules." Colloids and Surfaces A: Physicochemical and Engineering Aspects 431: 142-149.

Liu, F. and C.-H. Tang (2016). "Soy glycinin as food-grade Pickering stabilizers: Part. I. Structural characteristics, emulsifying properties and adsorption/arrangement at interface." Food Hydrocolloids 60: 606-619.

Liu, F. and C.-H. Tang (2016). "Soy glycinin as food-grade Pickering stabilizers: Part. II. Improvement of emulsification and interfacial adsorption by electrostatic screening." Food hydrocolloids 60: 620-630.

Lo, C. and L. Ramsden (2000). "Effects of xanthan and galactomannan on the freeze/thaw properties of starch gels." Food/Nahrung 44(3): 211-214.

Naji-Tabasi, S., B. Emadzadeh and R. Kadkhodaee (2019). "Effects of pectin and xanthan gum on induced-flocculation phenomenon in an acidic model emulsion system." Journal of Dispersion Science and Technology 40(2): 256-263.

Naji-Tabasi, S. and S. M. A. Razavi (2016). "New studies on basil (Ocimum bacilicum L.) seed gum: Part II—Emulsifying and foaming characterization." Carbohydrate polymers 149: 140-150.

Naji-Tabasi, S., & Razavi, S. M. A. (2017). New studies on basil (Ocimum bacilicum L.) seed gum: Part III–Steady and dynamic shear rheology. Food Hydrocolloids, 67, 243-250.

Naji-Tabasi, S., S. M. A. Razavi and H. Mehditabar (2017). "Fabrication of basil seed gum nanoparticles as a novel oral delivery system of glutathione." Carbohydrate polymers 157: 1703-1713.

Naji-Tabasi, S., S. M. A. Razavi, M. Mohebbi and B. Malaekeh-Nikouei (2016). "New studies on basil (Ocimum bacilicum L.) seed gum: Part I-Fractionation, physicochemical and surface activity characterization." Food Hydrocolloids 52: 350-358.

Nourbehesht, N., Shekarchizadeh, H., & Soltanizadeh, N. (2018). Investigation of stability, consistency, and oil oxidation of emulsion filled gel prepared by inulin and rice bran oil using ultrasonic radiation. Ultrasonics sonochemistry, 42: 585-593.

Orouji, I., Dhanbarzadeh,B., and Danesh, E.(2017). Study of texture and sensory properties of prebiotic cream containing inulin and polydextrose by using response surface methodology. Journal of food research, 27(4), 193-207.‎

Pawlik, A., D. Kurukji, I. Norton and F. Spyropoulos (2016). "Food-grade Pickering emulsions stabilised with solid lipid particles." Food & function 7(6): 2712-2721.

Piraprez, G., Marie France Hérent and S. Collin (1998). "Determination of the lipophilicity of aroma compounds by RPHPLC." Flavour and fragrance journal 400-408.

Rayner, M., A. Timgren, M. Sjöö and P. Dejmek (2012). "Quinoa starch granules: a candidate for stabilising food‐grade Pickering emulsions." Journal of theScience of Food and Agriculture 92(9): 1841-1847.

Sahan, N., K. Yasar and A. Hayaloglu (2008). "Physical, chemical and flavour quality of non-fat yogurt as affected by a β-glucan hydrocolloidal composite during storage." Food Hydrocolloids 22(7): 1291-1297.

Shao, Y. and C.-H. Tang (2016). "Gel-like pea protein Pickering emulsions at pH 3.0 as a potential intestine-targeted and sustained-release delivery system for β-carotene." Food research international 79: 64-72.

Steffe, J. F. (1996). Rheological methods in food process engineering, Freeman press.

Williams, P. D., L. N. Sadar and Y. Martin Lo (2009). "Texture stability of hydrogel complex containing curdlan gum over multiple freeze–thaw cycles." Journal of Food Processing and Preservation 33(1): 126-139.

Wu, M., Xiong, Y. L., & Chen, J. (2011). Rheology and microstructure of myofibrillar protein–plant lipid composite gels: Effect of emulsion droplet size and membrane type. Journal of food engineering, 106(4), 318-324.

Yang, Y., Z. Fang, X. Chen, W. Zhang, Y. Xie, Y. Chen, Z. Liu and W. Yuan (2017). "An overview of Pickering emulsions: solid-particle materials, classification, morphology, and applications." Frontiers in pharmacology 8: 287.