با همکاری انجمن علوم و صنایع غذایی ایران

نوع مقاله : مقاله پژوهشی فارسی

نویسندگان

1 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، ایران

2 مؤسسه دولتی ارزیابی ریسک آلمان، برلین، آلمان

چکیده

امروزه به‌دلیل وجود نگرانی‌های زیست‌محیطی و افزایش تقاضای مصرف‌کنندگان برای محصولات غذایی با کیفیت و ماندگاری بیشتر، استفاده از فیلم‌ها و پوشش‌های زیست تخریب‌پذیر مورد توجه بسیاری واقع شده‌اند. در این مطالعه، اثر افزودن درصدهای مختلف نانوالیاف سلولز به فیلم مرکب ژلاتین-پلولان و اثر ضدباکتریایی فیلم‌های حاوی باکتریوفاژ توسط روش انتشار دیسک بررسی شد. به‌علاوه، اثر ضدباکتریایی فیلم مرکب ژلاتین-پلولان-نانو فیبر سلولز روی گوشت مرغ در طول دوره نگه‌داری در دو دمای 4 و 12 درجه سانتی‌گراد علیه باکتری سالمونلا تایفی موریوم مورد مطالعه قرار گرفت. نتایج نشان داد که با افزایش درصد نانوفیبرهای سلولز در فیلم ژلاتین-پلولان ضخامت، حلالیت، تورم، مقاومت کششی، و درصد کشش پذیری فیلم‌ها به‌ترتیب افزایش، کاهش، افزایش، افزایش، و کاهش یافتند. فیلم‌های حاوی باکتریوفاژ روی محیط آگار ناحیه بازدارندگی خوبی داشتند. استفاده از فیلم ضدباکتریایی روی سطح گوشت مرغ در دمای  12 بعد از یک روز منجر به کاهش یک سیکل لگاریتمی شد در حالی‌که در دمای  4 در روز هفتم یک سیکل لگاریتمی کاهش را در جمعیت باکتری سالمونلا منجر شد.  

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Production of Gelatin-Pullulan- Nanofibers Cellulose Film Containing Salmonella Phages and Effect Its Anti-salmonella Against Salmonella typhimurium

نویسندگان [English]

  • Asma Entezari 1
  • Nasser Sedaghat 1
  • Golshan Shakeri 2

1 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran

2 German Federal Institute for Risk Assessment (BfR), Department of Chemicals and Product Safety, Berlin, German

چکیده [English]

Introduction
 The main sources of Salmonella for humans are pork, beef, chicken, eggs, fruits, vegetables, and their derivatives such as mayonnaise, and peanut butter. Different species of Salmonella can adapt, grow or survive at different environmental conditions. Salmonella enterica is a majorcause of food borne illness in humans, and Salmonella typhimurium and Salmonella enteritidis serovars are the most prevalent. One strategy is to use active packaging to reduce the microbial load or prevent the growth of microorganisms on food. Recently, antimicrobial active packaging has received much attention due to maintaining food quality, safety, and increasing shelf life. Among the antimicrobials used in the food industry, bacteriophages have a very good efficiency to control pathogenic bacteria. Pullulan has a good ability to form a film, its film has good characteristics such as transparency, odorlessness, tastelessness, solubility in water, and low permeability to oxygen and fat, However, the major obstacle is related to its price. The combination of polysaccharides with proteins has been done in order to improve the performance and reduce the costs of films. Gelatin is a suitable option to combine with pullulan in terms of good mechanical properties, reduced permeability, and its good price. Different ratios of gelatin and pullulan were studied and suitable film selected, but it needed to modify, so nanofibers cellulose was added in order to improve the mechanical properties and water resistance. Adding cellulose nano fiber can be a good and appropriate option. The aim of this research was to evaluate theantibacterial effectiveness of gelatin-pullulan- nanofibers cellulose composite film containing bacteriophage against Salmonella typhimurium at two different temperatures.
 
Materials and Methods
Gelatin and pullulan powders were weighted separately and mixed together (20gelatin-80pullulan). Nanofiber cellulose was extracted from rice bran and was used at three different levels (1%, 3%, and 5%). Commercial bacteriophage solution was added to each of the films separately and the films were prepared by molding method. Thickness, moisture content, solubility, swelling, tensile strength, and elongation of gelatin-pullulan film containing nanofibers were studied. Zone inhibitory of films containing different percentage of cellulose nanofibers on the agar media against Salmonella typhimurium (104 CFU.ml-1) was evaluated. The, antibacterial effect of selected film on the poultry meat inoculated with S. typhimurium (104 CFU.g-1) and several phages on the surface meat at 4  and 12  was also investigated. 
Results and Discussion
 The results indicated that gelatin-pullulan films containing different percentages of cellulose were showed approximately 2 mm of zone inhibitory compare to films free of phages. Also, inhibitory among films at different percentage of nanofiber cellulose did not show significant change. Antibacterial effect on poultry meat was dependent on temperature, films loaded with bacteriophages at higher temperature (12 ) was more effective compare to lower temperature (4 ). The populations of S. typhimurium were decreased 1 log and 0.7 log than control samples at 4  after 7 and 9 days respectively, while at 12 , 1 log and around 2.55 log decrease was found after 1 and 9 days, respectively. In a study, beef inoculated with salmonella was treated by SALMONELEXTM bacteriophage and resulted in 1.29 log reduction of pathogenic bacteria compared to the control sample (Yeh et al., 2017).  In another study, the antibacterial effect of double-layer poly lactic acid/xanthan film at 10 °C compared to 4 °C against pathogenic bacteria of Salmonella and Listeria was determined and found that at10 °C, the number of pathogenic bacteria was decreased more than at 4 °C (Radford et al., 2017).. Kamali et al. (2022b) reported that the release of phages from the film of 30 poly lactic acid/70 whey protein to the meat surface after one hour was 63.22 % and 63.18 % at 4 °C and 10 °C, respectively, which means no  significant difference, after one day at both temperatures.







 

کلیدواژه‌ها [English]

  • Bio-control
  • Composite film
  • Food safety
  • Pathogen bacteria

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

  1. Alves, D., Cerqueira, M.A., Pastrana, L.M., & Sillankorva, S. (2020). Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella enteritidis. Food Research International, 128, 108791. https://doi.org/10.1016/j.foodres.2019.108791
  2. Alves, D., Marques, A., Milho, C., Costa, M.J., Pastrana, L.M., Cerqueira, M.A., & Sillankorva, S.M. (2019). Bacteriophage ϕIBB-PF7A loaded on sodium alginate-based films to prevent microbial meat spoilage. International Journal of Food Microbiology, 291(16), 121-127. https://doi.org/10.1016/j.ijfoodmicro.2018.11.026
  3. (2014). Standard Test Method for Tensile Properties of Plastics. In ASTM International (Vol. ASTM D638-14, pp. 1–15). West Conshohocken: PA, USA.
  4. Besbes, I., Alila, S., & Boufi, S. (2010). Nanofibrillated cellulose from TEMPO-oxidized eucalyptus fibres: Effect of the carboxyl content. Carbohydrate Polymers, 84(3), 975–983. https://doi.org/10.1016/j.carbpol.2010.12.052
  5. Chen, F., & Chi, C. (2022). Development of pullulan/carboxylated cellulose nanocrystal/tea polyphenol bionanocomposite films for active food packaging. International Journal of Biological Macromolecules, 186(1), 405–413. https://doi.org/10.1016/j.ijbiomac.2021.07.025
  6. García-Anaya, M., R.Sepúlveda, D., ClaudioRios-Velasco, B.Zamudio-Flores, P., I.Sáenz-Mendoza, A., & H.Acosta-Muñiz, C. (2020). The role of food compounds and emerging technologies on phage stability. Innovative Food Science & Emerging Technologies, 64, 102436. https://doi.org/10.1016/j.ifset.2020.102436
  7. Dicastillo, C.L., Settier-Ramírez, L., Gavara, R., Hernández-Muñoz, P., & Carballo, G.L. (2021). Development of biodegradable films loaded with phages withantilisterial properties. Polymers, 13(3), 327. https://doi.org/10.3390/polym13030327
  8. Faraji, S., Maghsoudlou, Y., Khomeiri, M., Kashiri, M., & Babaei, A. (2019). In vitro biocontrol of Escherichia coli through the immobilization of its specific lytic bacteriophage on cellulose acetate biodegradable film. Iranian Journal of Medical Microbiology, 12(6), 399-408. https://doi.org/30699/ijmm.12.6.399
  9. González, A., Gastelú, G., Barrera, G.N., Ribotta, P.D., & Igarzabal, C.I.Á. (2018). Preparation and characterization of soy protein films reinforced with cellulose nanofibers obtained from soybean by-products. Food Hydrocolloids, 89, 758-764. https://doi.org/10.1016/j.foodhyd.2018.11.051
  10. Gouvea, D.M., Mendonça, R.C.S., Soto, M.L., & Cruz, R.S. (2015). Acetate cellulose film with bacteriophages for potential antimicrobial use in food packaging. LWT - Food Science and Technology, 63(1), 85-91. https://doi.org/10.1016/j.lwt.2015.03.014
  11. Guenther, S., Herzig, O., Fieseler, L., Klumpp, J., & Loessner, M.J. (2012). Biocontrol of Salmonella typhimurium in RTE foods with the virulent bacteriophage FO1-E2. International Journal of Food Microbiology, 154(1-2), 66-77. https://doi.org/10.1016/j.ijfoodmicro.2011.12.023
  12. Jahed, E., Almasi, H., & Khaledabad, M.A. (2019). Producing and optimizing the properties of chitosan-organic nanofiber biodegradable nanocomposite based containing vulgare subsp. gracile and C. copticum essential oils and its application on the oxidative stability of Canola oil. Iranian Food Science and Technology, 14(5), 907-927. https://doi.org/10.22067/ifstrj.v14i5.71229
  13. Kamali, S., Yavarmanesh, M., Najafi, M.B.H., & Koocheki, A. (2022a). Development of whey protein concentrate/pullulan composite films containing bacteriophage A511: Functional properties and anti-Listerial effects during storage. Food Packaging and ShelfLife, 33, 100902-100917. https://doi.org/10.1016/j.fpsl.2022.100902
  14. Kamali, S., Yavarmanesh, M., Najafi, M.B.H., & Koocheki, A. (2022b). Poly (lactic acid) and whey protein/pullulan composite bilayer film containing phage A511 as an anti-Listerial packaging for chicken breast at refrigerated temperatures. LWT- Food Science and Technology, 170, 114085. https://doi.org/10.1016/j.lwt.2022.114085
  15. Kassab, Z., Aziz, F., Hannache, H., Youcef, H.B., & Achaby, M.E. (2019). Improved mechanical properties of k-carrageenan-based nanocomposite films reinforced with cellulose nanocrystals. International Journal of Biological Macromolecules, 123, 1248-1256. https://doi.org/10.1016/j.ijbiomac.2018.12.030
  16. Khodaei, D., Oltroggea, K., & Hamidi-Esfahani, Z. (2020). Preparation and characterization of blended edible films manufactured using gelatin, tragacanth gum and, Persian gum. LWT- Food Science and Technology, 117, 108617. https://doi.org/10.1016/j.lwt.2019.108617
  17. Kowalczyk, D., MonikaKordowska-Wiater, Karas, M., Zięba, E., MonikaMężyńska, & Wiącek, A.E. (2020). Release kinetics and antimicrobial properties of the potassium sorbate-loaded edible films made from pullulan, gelatin and their blends. Food Hydrocolloids, 101, 105539. https://doi.org/1016/j.foodhyd.2019.105539
  18. Leung, V., Szewczyk, A.Y., Chau, J., Hosseini-Doust, Z., Groves, L., Hawsawi, H., Filipe, C.D.M. (2017). Long-term preservation of bacteriophage antimicrobials using sugar glasses. ACS Biomaterials Science and Engineering, 4(11), 3802-3808. https://org/10.1021/acsbiomaterials.7b00468
  19. Miraghaei, S., & Cheguini, F.K. (2014). Paper presented at the International Conference on Natural Food Hydrocolloids, Mashhad.
  20. Morcillo-Martín, R., Espinosa, E., Rabasco-Vílchez, L., Sanchez, L.M., Haro, J.., & Rodríguez, A. (2022). Cellulose nanofiber-based aerogels from wheat straw: influence of surface load and lignin content on their properties and dye removal capacity. Biomolecules, 12(2), 32. https://org/10.3390/biom12020232
  21. Radford, D., Guild, B., Strange, P., Ahmed, R., Lim, L.-T., & Balamurugan, S. (2017). Characterization of antimicrobial properties of Salmonella phage Felix O1 and Listeria phage A511 embedded in xanthan coatings on Poly (lactic acid) films. Food Microbiology, 66, 117-128. https://org/10.1016/j.fm.2017.04.015
  22. Ratna, Aprilia, S., Arahman, N., Bilad, M.R., Suhaimi, H., Munawar, A.A., & Nasution, I.S. (2022). Bio-nanocomposite based on edible gelatin film as active packaging from Clarias gariepinus fish skin with the addition of cellulose nanocrystalline and nanopropolis. Polymers, 14(18), 3738. https://org/10.3390/polym14183738
  23. Robeson, J., Turra, G., Huber, K., & Borie, C. (2014). A note on stability in food matrices of Salmonella enterica serovar Enteritidis-controlling bacteriophages. Electronic Journal of Biotechnology, 17(4), 189-191. https://doi.org/10.1016/j.ejbt.2014.06.001
  24. Roy, S., Biswas, D., & Rhim, J.-W. (2022). Gelatin/cellulose nanofiber-based functional nanocomposite film incorporated with zinc oxide nanoparticles. Journal Composites Science, 6(8), 223. https://org/10.3390/jcs6080223
  25. Roy, S., & Rhim, J.W. (2022). Gelatin/cellulose nanofber‑based functional flms added with mushroom‑mediated sulfur nanoparticles for active packaging applications. Journal of Nanostructure in Chemistry, 12, 979–990. https://org/10.1007/s40097-022-00484-3
  26. Sezer, B., Tayyarcan, E.K., & Boyaci, I.H. (2022). The use of bacteriophage-based edible coatings for the biocontrol of Salmonella in strawberries. Food Control, 135(101812). https://doi.org/10.1016/j.foodcont.2022.108812
  27. Shabanpour, B., Kazemi, M., Ojagh, S.M., & Pourashouri, P. (2018). Bacterial cellulose nanofibers as reinforce in edible fish myofibrillar protein nanocomposite films. International Journal of Biological Macromolecules, 117(1), 742-751. https://doi.org/10.1016/j.ijbiomac.2018.05.038
  28. Shakeri, G., Hammerl, J.A., Jamshidi, A., Ghazvini, K., Rohde, M., Szabo, I., & Kittler, S. (2021). The lytic siphophage vB_StyS-LmqsSP1 reduces the number of Salmonella enterica serovar Typhimurium isolates on chicken skin. Appllied and Environmental Microbiology, 87(24), e01424-01421. https://org/10.1128/AEM.01424-21
  29. Trovatti, E., Fernandes, S.C.M., Rubatat, L., Perez, D.S., Freire, C.S.R., Silvestre, A.J.D., & Neto, C.P. (2012). Pullulan–nanofibrillated cellulose composite films with improved thermal and mechanical properties. Composites Science and Technology, 72(13), 1556–1561. https://doi.org/10.1016/j.compscitech.2012.06.003
  30. Vonasek, E., Le, P., & Nitin, N. (2014). Encapsulation of bacteriophages in whey protein films for extended storage and release. Food Hydrocolloids, 37, 7-13. https://doi.org/10.1016/j.foodhyd.2013.09.017
  31. Wang, W., Liu, Y., Jia, H., Liu, Y., Zhang, H., He, Z., & Ni1, Y. (2017). Effects of cellulose nanofibers filling and palmitic acid emulsions coating on the physical properties of fish gelatin films. Food Biophysics, 12, 23-32. https://org/10.1007/s11483-016-9459-y
  32. Weng, S., Lopez, A., Saez-Orviz, S., Marcet, I., García, P., Rendueles, M., & Díaz, M. (2021). Effectiveness of bacteriophages incorporated in gelatine films against Staphylococcus aureus. Food Control, 121, 1-10. https://doi.org/10.1016/j.foodcont.2020.107666
  33. Yeh, Y., Moura, F.H.., Broek, K.V.D., & Mello, A.S. (2018). Effect of ultraviolet light, organic acids, and bacteriophage on Salmonella populations in ground beef. Meat Science, 139, 44-48. https://org/10.1016/j.meatsci.2018.01.007
  34. Yang, Y., Xie, B., Liu, Q., Kong, B., & Wang, H. (2020). Fabrication and characterization of a novel polysaccharide based composite nanofiber films with tunable physical properties. Carbohydrate Polymers, 236(15), 116054. https://org/10.1016/j.carbpol.2020.116054
  35. Yeh, Y., Purushothaman, P., Gupta, N., Ragnone, M., Verma, S.C., & Mello, A.S. (2017). Bacteriophage application on red meats and poultry: Effects on Salmonella population in final ground products. Meat Science, 127, 30-34. https://org/10.1016/j.meatsci.2017.01.001
  36. Zhang, C., Gao, D., Ma, Y., & Zhao, A.X. (2013). Effect of gelatin addition on properties of pullulan films. Journal of Food Science, 78(6), C805-C810. https://org/10.1111/j.1750-3841.2012.02925.x

 

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