نوع مقاله : مقاله پژوهشی
نویسندگان
1 پژوهشکده اکولوژی دریای خزر، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری، ایران
2 گروه فراوری محصولات شیلاتی، دانشکده شیلات و محیطزیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
چکیده
جهت استخراج رنگدانهها از جلبکهای دریایی از تکنیکهای مختلفی استفاده میشود که هر تکنیک علاوه بر داشتن معایب و مزایایی، راندمان متفاوتی دارد. یکی از این رنگدانهها، فیکوسیانین است که از روشهای مختلفی جهت استخراج آن استفاده میگردد. هدف پژوهش حاضر کشت میکروجلبک اسپیرولینا پلاتنسیس و استخراج فیکوسیانین از آن با استفاده از چهار روش اولتراسوند، انجماد-انجمادزدایی، آنزیمی و حلال معدنی بود. در مراحل بعد، میزان راندمان هر روش از طریق اندازهگیری غلظت و خلوص فیکوسیانین ارزیابی شد. ضمن اینکه اثر اعمال فرایند خالصسازی با سولفات آمونیوم نیز بر غلظت و خلوص رنگدانه مستخرج مورد سنجش قرار گرفت. نتایج نشان داد رشد میکروجلبک از زمان صفر تا 14 روز، دارای روند صعودی و تغییرات حاصله نیز در تمامی زمانها، بجز روزهای 14 و 16، معنیدار بوده است (05/0>p). همچنین جلبک بعد از سپریکردن فاز سکون کوتاه (2 روز)، وارد مرحله رشد لگاریتمی شد و تا روز 14 به رشد خود ادامه داد اما بین روزهای 14 و 16، رشد تقریبا روند ثابتی بخود گرفت. در ادامه مشخص شد که میزان توده تولیدشده پس از 16 روز، 1120 میلیگرم بر لیتر میباشد. غلظت فیکوسیانین استخراجشده در روشهای آنزیمی و اولتراسوند (به ترتیب 815/1 و 786/1 میلیگرم در میلیلیتر) فاقد اختلاف معنیدار (05/0<p) و در سطح بالاتری از دو روش دیگر قرار داشت (05/0>p)؛ ضمن اینکه غلظت رنگدانه در تکنیک انجماد-انجمادزدایی (535/1 میلیگرم در میلیلیتر) بیشتر از روش حلال معدنی (121/1 میلیگرم در میلیلیتر) بود (05/0>p). پس از خالصسازی رنگدانه با استفاده از سولفات آمونیوم، غلظت و خلوص رنگدانه به صورت معنیداری در هر روش افزایش یافت (05/0>p). نتایج تحقیق حاضر نشان داد که با انتخاب روش بهینه و همچنین اعمال فرایند خالصسازی با سولفات آمونیوم، میتوان راندمان استخراج فیکوسیانین از میکروجلبک اسپیرولینا پلاتنسیس را افزایش داد.
کلیدواژهها
موضوعات
©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.
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