نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

1 گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه تخصصی فن‌آوری‌های نوین آمل، آمل، ایران.

2 گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه تخصصی فن‌آوری‌های نوین آمل، آمل، ایران

چکیده

ترخینه، غذای تخمیری سنتی ایران بر پایه غلات، از مخلوطی از آرد غلات، سبزیجات، سبزی، ادویه‌جات و ماست تهیه می‌شود. هدف از این مطالعه بهبود بقای باکتری‌های اسید لاکتیک (LAB) در سوپ ترخینه به‌عنوان یک ماتریکس غیرلبنی بود. لاکتوباسیلوس دلبروکئی زیرگونه بولگاریکوس و استرپتوکوکوس ترموفیلوس در نانوالیاف الکتروریسی شده تهیه شده از نشاسته ذرت (CS) و سدیم آلژینات (SA) ریزپوشانی شدند و اثر محافظتی نانوالیاف بر سلول‌ها در طول تهیه ترخینه و نیز در دستگاه گوارش مورد بررسی قرار گرفت. نتایج نشان داد که مقدار پتانسیل زتا 1/15- میلی ولت برای نانوالیاف بارگذاری شده با LAB مشاهده شد. نانوالیاف حاوی LAB دارای ساختار مهره‌ای با توزیع یکنواخت و قطر متوسط 763 نانومتر بودند. بقای لاکتوباسیلوس دلبروکئی و استرپتوکوکوس ترموفیلوس در پایان الکتروریسی 82/92 و 83/95 درصد بود که نشان‍دهنده کاهش جزئی در جمعیت آنها است. بقای  استرپتوکوکوس ترموفیلوس و لاکتوباسیلوس دلبروکئی نانوکپسوله شده 50/93و 16/89 درصد بود، در حالی که برای سلول‌های آزاد 3/85 و 4/76 درصد بود که اثر محافظتی قابل توجه الیاف CS/SA را بر سلول‌ها در برابر دهیدراتاسیون محیط ترخینه نشان داد. نانوالیاف پایداری قابل توجهی برای سلول‌ها در برابر فرآیند حرارتی تهیه سوپ و همچنین در دستگاه گوارش فراهم آوردند، زیرا بقای 106 تا 107 سلول تامین شد که در محدوده دوز توصیه‌شده پروبیوتیک جهت تاثیرگذاری بر سلامت مصرف‌کننده قرار دارد. مصرف‌کنندگانی مانند گیاه‌خواران و افراد مبتلا به عدم تحمل لاکتوز/ پپتید شیر که لبنیات مصرف نمی‌کنند، لیکن به مصرف مواد غذایی پروبیوتیکی نیاز دارند، می‌توانند از این محصول استفاده نمایند.

کلیدواژه‌ها

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