نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
2 گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
امروزه تمایل به تولید و استفاده از مواد با ابعاد نانومتری با توجه به ویژگیهای منحصر به فرد و جالب این مواد روز به روز در حال افزایش است. تاکنون روشهای مختلف فیزیکی و شیمیایی جهت سنتز نانو ذرات نقره مورد استفاده قرار گرفته است اما استفاده از گیاهان جهت سنتز نانو ذرات نقره بسیار سریع، ساده، غیرسمی و سازگار با محیط زیست است. در این پژوهش، عصاره آبی برگ گیاه شیشهشور جهت سنتز زیستی نانو ذرات نقره مورد استفاده قرار گرفت. رنگ محلول نیترات نقره پس از افزودن عصاره به رنگ مایل به قرمز تغییر رنگ داد. فعالیت ضدمیکروبی نانو ذرات نقره علیه باکتریهای گرم مثبت استافیلوکوکوس اورئوس و لیستریا اینوکوا و باکتریهای گرم منفی اشرشیاکلی، سودوموناس آئروژینوزا و سالمونلا تیفی با روشهای انتشار در دیسک، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی مورد بررسی قرار گرفت. حداقل غلظت مهارکنندگی برای باکتریهای اشرشیاکلی، استافیلوکوکوس اورئوس، سودوموناس آئروژینوزا، سالمونلا تیفی، لیستریا اینوکوا بهترتیب 128، 256، 256، 256 و 512 میلیگرم بر میلیلیتر بود و حداقل غلظت کشندگی نانو ذرات نقره برای تمامی باکتریها بزرگتر از 512 میلیگرم بر میلیلیتر بود. قطر هاله عدم رشد برای باکتری سودوموناس آئروژینوزا (حساسترین سویه) در روشهای انتشار در دیسک و چاهک آگار در غلظت 150 میلیگرم بر میلیلیتر بهترتیب 13 و 75/7 میلیمتر بود. در حالی که قطر هاله عدم رشد برای باکتری استافیلوکوکوس اورئوس (مقاومترین سویه) در روشهای انتشار در دیسک و چاهک آگار در غلظت 150 میلیگرم بر میلیلیتر بهترتیب 8 و 75/6 میلیمتر بود. نتایج این پژوهش نشان داد که عصاره برگ گیاه شیشهشور قادر به سنتز نانو ذرات نقره میباشد و نانو ذرات سنتزی فعالیت ضدمیکروبی مناسبی بر سویههای بیماریزا در شرایط برونتنی از خود نشان داد.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Green synthesis of silver nanoparticles using Callistemon citrinus leaf extract and evaluation of its antibacterial activity
نویسندگان [English]
- Zohreh Sosani Gharibvand 1
- Behrooz Alizadeh Behbahani 1
- Mohammad Noshad 2
- Hossein Jooyandeh 1
1 Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده [English]
Introduction: Nowadays, production and utilization of Nano materials have increased due to their unique and interesting properties. So far, different physical and chemical methods have been used to synthesize silver nanoparticles. Chemical synthesis is not compatible due to the hazardous chemicals residues on the surface of the nanoparticles (NP) as well as production of by products with high impact on the environment. Physical routes for synthesis of NPs have some drawbacks, too. These methods require high energy and space, and are expensive. Therefore, biological methods for the synthesis of silver nanoparticles are considered emerging technologies as economic choices in the green chemistry field. Among these methods, plant-mediated synthesis of AgNPs is a rapid, simple, non-toxic and eco-friendly technique. Silver nanoparticles exhibit high bactericidal activity at their utilized concentrations with no toxic effect on human cells, and they also strongly enhance the antibacterial activity of conventional antibiotics even against multi-resistant bacteria through their synergistic effects. Callistemon citrinus belongs to the family Myrtaceae and includes more than 30 species. The plant is widespread in wet tropics, notably Australia, South America and tropical Asia, but presently can be found all over the world. Callistemon citrinus is a potential medicinal plant used to treat gastrointestinal distress, pain, and infectious diseases caused by bacteria, fungi, viruses, and parasites. In this study Callistemon citrinus aqueous extract was used to reduce silver ions in silver nitrate solution. In the following, the antimicrobial activity of nanoparticles synthesized by various qualitative and quantitative methods on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi and Listeria innocua was investigated.
Materials and Methods: For the synthesis of silver nanoparticles, 25 mL of silver nitrate solution was added to 5 mL of leaf extract with a concentration of 100 mg/mL and maintained for 24 h at 20 °C. Change the color of the solution to Red represents the production of silver nanoparticles in the solution. To stabilize the presence of silver nanoparticles, the absorption spectrum of silver nanoparticles produced by spectrophotometer was prepared. Antimicrobial activity of silver nanoparticles synthesized using Callistemon citrinus leaf aqueous extract was examined by disc diffusion agar, well diffusion agar, minimum inhibitory concentration (microdilution broth) and minimum bactericidal concentration on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi and Listeria innocua.
Results and Discussion: The results showed that in disc diffusion agar method, the diameter inhibition zone increased with increasing the concentration of silver nanoparticles. The maximum effect of silver nanoparticles synthesized using Callistemon citrinus leaf aqueous extract at a concentration of 150 mg / ml was observed for Pseudomonas aeruginosa. An inhibition zone was observed for all examined pathogenic microorganisms at all concentrations. The results showed that in the well diffusion agar method, nanosilver particles at a concentration of 18.75 mg/ml did not show any inhibitory effect on all the pathogenic microorganisms. The results of statistical analysis showed that there was no significant difference between all the concentrations of silver nanoparticles synthesized for Escherichia coli, Salmonella typhi and Staphylococcus aureus (P˂ 0.05(. The MIC for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhimurium and Listeria innocua was 128, 256, 256, 256 and 512 mg/mm, respectively. The MBC for all the pathogenic strains was 512 mg/mm. The results of this study showed that the Callistemon citrinus leaf extract has a good ability to synthesize silver nanoparticles. Nanoparticles synthesized from Callistemon citrinus leaf extract had good antimicrobial activity against examined pathogenic bacteria, especially Gram-negative bacteria. Green-synthesized nanoparticles can be used as antimicrobial agent to fight infectious diseases caused by various microbial strains, although more research is needed in vitro, animal models and in vivo.
کلیدواژهها [English]
- Aqueous extract
- Callistemon citrinus leaf
- Silver nanoparticles
- Gram-positive and Gram-negative bacteria
- Abbasi, E., Milani, M., Fekri Aval, S., Kouhi, M., Akbarzadeh, A., Tayefi Nasrabadi, H., & Samiei, M., (2016). Silver nanoparticles: synthesis methods, bio-applications and properties. Critical Reviews in Microbiology, 42(2), 173-180. https://doi.org/10.3109/1040841X.2014.912200
- Ahluwalia, V., Elumalai,, Kumar, V., Kumar, S., & Sangwa, R. S., (2018). Nano silver particle synthesis using Swertia paniculata herbal extract and its antimicrobial activity. Microbial Pathogenesis, 114, 402-408. https://doi.org/10.1016/j.micpath.2017.11.052
- Alghooneh, A., Alizadeh Behbahani, B., Noorbakhsh, H., Tabatabaei Yazdi, F. (2015). Application of intelligent modeling to predict the population dynamics of Pseudomonas aeruginosa in Frankfurter sausage containing Satureja bachtiarica extracts. Microbial Pathogenesis, 85,58-65. https://doi.org/10.1016/j.micpath.2015.06.003
- Alizadeh Behbahani, B., Noshad, , Falah, F. (2019). Study of chemical structure, antimicrobial, cytotoxic and mechanism of action of syzygium aromaticum essential oil on foodborne pathogens. Potravinarstvo Slovak Journal of Food Sciences,13(1), 875-83. https://doi.org/10.5219/1226
- Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Mortazavi, SA., Zendeboodi, F., Gholian, MM., Vasiee, A. (2013). Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis on food infection and intoxication microorganisms “in vitro”. Journal of Paramedical Sciences, 4(3), 89-99. https://doi.org/10.22037/jps.v4i3.4666
- Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Noorbakhsh, H., Riazi, F., Jajarmi, A., Tabatabaei Yazdi, F. (2016). Study of the antibacterial activity of methanolic and aqueous extracts of Myrtus communis on pathogenic strains causing infection. Zahedan Journal of Research in Medical Sciences, 18(2), e5989. doi: 10.17795/zjrms-5989
- Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Shahidi, F., & Riazi, F. (2016). Antifungal Effect of the Aqueous and Ethanolic Avicennia marina Extracts on Alternaria citri and Penicillium digitatum. Zahedan Journal of Research in Medical Sciences, 18(2), e5992. doi: 10.17795/zjrms-5992
- Arokiyaraj, S., Arasu, MV., Vincent, S., Prakash, NU., Choi, SH., Oh, YK., Choi, KC., & Kim, KH., (2014). Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study. International Journal of Nanomedicine, 9, 379-378.
- Azizian Shermeh, O., Valizadeh, J., Noroozifar, M., & Qasemi, A., (2016). Investigating the Antimicrobial Activities of Silver Nanoparticles Biosynthesized by Aqueous Extract of Sambucus ebulus L. Scientific Journal of Ilam University of Medical Sciences, 24(5), 92-108. ]In Persian[.
- Balasundaram, A., Ragupathy, R., Sankar, S., Thiyagarajan, M., Ravi, L., Karuppasamy, R., & Veerappapillai, S., (2016). Investigation of Phytocompounds and Computational Approach for the Evaluation of Therapeutic Properties of Ethanolic Leaf Extract of Callistemon citrinus. International Journal of Pharmaceutical Sciences, 37(1), 110-116.DOI: 2147/IJN.S53546
- Behravan, M., Panahi, AH., Naghizadeh, A., Ziaee, M., Mahdavi, R., & Mirzapour, A., (2019). Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules, 124, 148-54. https://doi.org/10.1016/j.ijbiomac.2018.11.101
- Dehghan Nayeri, F., Mirhosseini, M., Mafakheri, S., & Zarrabi, MM., (2018). Antibacterial and antifungal effects of silver nanoparticles synthesized by the aqueous extract of sesame (Sesamum indicum L.). Journal of Cellular and Molecular Research (Iranian Journal of Biology), 31(1), 16-26. ]In Persian[.
- Dhand, V., Soumya, L., Bharadwaj, S., Chakra, S., Bhatt, D., & Sreedhar, B., (2016). Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Materials Science and Engineering: C, 58, 36-43. https://doi.org/10.1016/j.msec.2015.08.018
- Dolatabadi, S., Emrani, S., Mehrafruz, E., & Zhiani, R., (2017). Green synthesis and antibacterial effect of silver nanoparticles using Eucalyptus camaldulensis. Journal of Neyshabur University of Medical Sciences, 5(3), 74-85. ]In Persian.[
- Dousti, B., Nabipour, F., & Hajiamraei, A., (2019). Green synthesis of silver nanoparticle by using the aqueous extract of Fumaria Parviflora and investigation of their antibacterial and antioxidant activities. Razi Journal of Medical Sciences, 26(6), 105-117. ]In Persian[.
- Emrani, Sh., Zhiani, R., & DafeJafari, M., (2018). The Biosynthesis of Silver Nanoparticles Using Plants of Glycyrrhiza glabra and Mentha Piperata and Its Antimicrobial Effect on Some Bacterias That Cause Tooth Decay. Journal of Rafsanjan University of Medical Sciences, 16(10), 953-968. ]In Persian[.
- Erjaee, H., Rajaian, H., & Nazifi, S., (2017). Synthesis and characterization of novel silver nanoparticles using Chamaemelum nobile extract for antibacterial application. Advances in Natural Sciences: Nanoscience and Nanotechnology, 8(2), 1-9.
- Gomathi, M., Rajkumar, PV., Prakasam, A., & Ravichandran, K., (2017). Green synthesis of silver nanoparticles using Datura stramonium leaf extract and assessment of their antibacterial activity. Resource-Efficient Technologies, 3(3), 280-4. https://doi.org/10.1016/j.reffit.2016.12.005
- Guzman, M., Dille, J., & Godet, S., (2012). Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine: Nanotechnology, Biology and Medicine, 8(1), 37-45. https://doi.org/10.1016/j.nano.2011.05.007
- Haji Rostamloo, B., Zhiyani, R., Omrani, Sh., (2019). Biosynthesis of Silver Nanoparticles by Salvia officinalis Extract and Evaluation of their Antioxidant and Antimicrobial Activity Against Bacteria of Food Corruption. Journal of Innovation in Food Science and Technology, 11(2), 107-118. ]In Persian[.
- Karamian, R., & Kamalnejade, J., (2019). Green synthesis of silver nanoparticles using aqueous seed extract of Cuminum cyminum L. and evaluation of their biological activities. Scientific Journal of Ilam University of Medical Sciences, 10;26(5), 128-41. ]In Persian[.
- Kavoosi, S., & Yaghoubi, H., (2017). Synthesis of silver nanoparticles using green method of plant extract European marjoram (Origanum majorana) and their antibacterial effects. Journal of Cellular and Molecular Research (Iranian Journal of Biolohy), 30(2), 161-173. ]In Persian[.
- Kiarsi, Z., Hojjati, M., Alizadeh Behbahani, B., Noshad, M. (2020). In vitro antimicrobial effects of Myristica fragrans essential oil on foodborne pathogens and its influence on beef quality during refrigerated storage. Journal of Food Safety 40(3), e12782. https://doi.org/10.1111/jfs.12782
- Larayetan, R., Ojemaye, MO., Okoh, OO., & Okoh, AI., (2019). Silver nanoparticles mediated by Callistemon citrinus extracts and their antimalaria, antitrypanosoma and antibacterial efficacy. Journal of Molecular Liquids, 273, 615-625. https://doi.org/10.1016/j.molliq.2018.10.020
- Larayetan, R., Ololade, ZS., Ogunmola, OO., & Ladokun, A., (2019). Phytochemical Constituents, Antioxidant, Cytotoxicity, Antimicrobial, Antitrypanosomal, and Antimalarial Potentials of the Crude Extracts of Callistemon citrinus. Evidence-Based Complementary and Alternative Medicine, 28, 1-14. https://doi.org/10.1155/2019/5410923
- Mabhiza, D., Chitemerere, T., & Mukanganyama, S., (2016). Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry, 1-7. http://dx.doi.org/10.1155/2016/6304163
- Mafakheri, S., Dehghan Nayeri, F., & Mirhoseini, M., 2017. Study the biological production and antibacterial and antifungal effects of silver nanoparticles synthesized by the methanolic extract of clove (Syzygium aromaticum). Modares Journal of Biotechnology, 8(3), 110-120. ]In Persian[.
- Mahadevan, S., Vijayakumar, S., & Arulmozhi, P., (2017). Green synthesis of silver nano particles from Atalantia monophylla (L) Correa leaf extract, their antimicrobial activity and sensing capability of H2O2. Microbial Pathogenesis, 113, 445-450. https://doi.org/10.1016/j.micpath.2017.11.029
- Nayak, D., Ashe, S., Rauta, PR., Kumari, M., & Nayak, B., (2016). Bark extract mediated green synthesis of silver nanoparticles: evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Materials Science and Engineering: C, 58, 44-52. https://doi.org/10.1016/j.msec.2015.08.022
- Nikbakht, M., & Pourali, P., (2015). Survey of biological and antibacterial effects of silver nanoparticles of aqueous and methanol extracts of Berberis Vulgaris. Medical Science Journal of Islamic Azad Univesity Tehran Medical, 25 (2), 112-118.
- Panacek, A., Kvitek, L., Smekalova, M., Vecerova, R., Kolar, M., Roderova, M., Dycka, F., Sebela, M., Prucek, R., Tomanec, O., & Zboril, R., (2018). Bacterial resistance to silver nanoparticles and how to overcome it. Nature Nanotechnology, 13(1), 65-71. https://doi.org/10.1038/s41565-017-0013-y
- Paosen, S., Saising, J., Septama, AW., & Voravuthikunchai, SP., (2017). Green synthesis of silver nanoparticles using plants from Myrtaceae family and characterization of their antibacterial activity. Materials Letters, 209, 201-6. https://doi.org/10.1016/j.matlet.2017.07.102
- Pirtarighat, S., Ghannadnia, M., & Baghshahi, S., (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry, 9(1), 1-9. https://doi.org/10.1007/s40097-018-0291-4
- Prabhu, S., & Poulose, EK., (2012). Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters, 2(32), 2-10. https://doi.org/10.1186/2228-5326-2-32
- Rafique, M., Sadaf, I., Tahir, MB., Rafique, MS., Nabi, G., Iqbal, T., & Sughra, K., (2019). Novel and facile synthesis of silver nanoparticles using Albizia procera leaf extract for dye degradation and antibacterial applications. Materials Science and Engineering C, 99, 1313-1324. https://doi.org/10.1016/j.msec.2019.02.059
- Rajeshkumar, S., & Bharath, LV., (2017). Mechanism of plant-mediated synthesis of silver nanoparticles–a review on biomolecules involved, characterisation and antibacterial activity. Chemico-Biological Interactions, 273, 219-27. https://doi.org/10.1016/j.cbi.2017.06.019
- Rasheed, T., Bilal, M., Iqbal, HM., & Li, Ch., (2017). Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids and Surfaces B: Biointerfaces, 158, 408-415. https://doi.org/10.1016/j.colsurfb.2017.07.020
- Rolim, WR., Pelegrino, MT., De Araujo Lima, B., Ferraz, LS., Costa, FN., Bernardes, JS., Rodigues, T., Brocchi, M., & Seabra, AB., (2019). Green tea extract mediated biogenic synthesis of silver nanoparticles: characterization, cytotoxicity evaluation and antibacterial activity. Applied Surface Science, 463, 66-74. https://doi.org/10.1016/j.apsusc.2018.08.203
- Singh, P., Kim, YJ., Singh, H., Wang, C., Hwang, KH., Farh, ME., & Yang, DC., (2015). Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. International Journal of Nanomedicine, 10, 2567-2577. DOI:2147/IJN.S72313
- Sosani Gharibvand, Z., Alizadeh Behbahani, B., Noshad, M., & Jooyandeh, H., (2020). Investigation of the Functional Groups of Bioactive Compounds, Radical Scavenging Potential, Antimicrobial Activity and Cytotoxic Effect of Callistemon Citrinus Aqueous Extract on Cell Line HT29: A Laboratory Study. Journal of Rafsanjan University of Medical Sciences, 19(5), 463-84. ]In Persian[. DOI:29252/jrums.19.5.463
- Suddin, R., & Akrema., (2016). Extracellular synthesis of silver dimer nanoparticles using Callistemon viminalis (bottlebrush) extract and evaluation of their antibacterial activity. An International Journal for Rapid Communication, 49(4), 268-75. https://doi.org/10.1080/00387010.2016.1140654
- Sureshjani, M. H., Tabatabaei Yazdi, F. Mortazavi, S. A., Alizadeh Behbahani, B., & Shahidi, F. (2014). Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria" in vitro”. Journal of Paramedical Sciences, 5(2), 115-120. https://doi.org/10.22037/jps.v5i2.5943
- Tolouietabar, H., & Hatamnia, AA., (2017). Investigation of antibacterial activity of silver nanoparticles synthesized from Scrophularia striata fruit extract. Journal of Cell & Tissue, 8(2), 206-213. ]In Persian[.
- Yeganegi, M., Tabatabaei Yazdi, F. Mortazavi, S. A., Asili, J., Alizadeh Behbahani, B., & Beigbabaei, A. (2018). Equisetum telmateia extracts: Chemical compositions, antioxidant activity and antimicrobial effect on the growth of some pathogenic strain causing poisoning and infection. Microbial Pathogenesis, 116, 62-67.https://doi.org/10.1016/j.micpath.2018.01.014
ارسال نظر در مورد این مقاله