سنتز سبز نانو ذرات نقره با استفاده از عصاره آبی برگ گیاه شیشه‌شور(Callistemon citrinus) و ارزیابی فعالیت ضدباکتریایی آن

نوع مقاله : مقاله پژوهشی فارسی

نویسندگان

• زهره سوسنی غریبوند ¹
• بهروز علیزاده بهبهانی ¹
• محمد نوشاد ²
• حسین جوینده ¹

¹ گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.

² گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران

https://doi.org/10.22067/ifstrj.2021.68173.1008

چکیده

امروزه تمایل به تولید و استفاده از مواد با ابعاد نانومتری با توجه به ویژگی‌های منحصر به فرد و جالب این مواد روز به روز در حال افزایش است. تاکنون روش‌های مختلف فیزیکی و شیمیایی جهت سنتز نانو ذرات نقره مورد استفاده قرار گرفته است اما استفاده از گیاهان جهت سنتز نانو ذرات نقره بسیار سریع، ساده، غیرسمی و سازگار با محیط زیست است. در این پژوهش، عصاره آبی برگ گیاه شیشه‌شور جهت سنتز زیستی نانو ذرات نقره مورد استفاده قرار گرفت. رنگ محلول نیترات نقره پس از افزودن عصاره به رنگ مایل به قرمز تغییر رنگ داد. فعالیت ضدمیکروبی نانو ذرات نقره علیه باکتری‌های گرم مثبت استافیلوکوکوس اورئوس و لیستریا اینوکوا و باکتری‌های گرم منفی اشرشیاکلی، سودوموناس آئروژینوزا و سالمونلا تیفی با روش‌های انتشار در دیسک، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی مورد بررسی قرار گرفت. حداقل غلظت مهارکنندگی برای باکتری‌های اشرشیاکلی، استافیلوکوکوس اورئوس، سودوموناس آئروژینوزا، سالمونلا تیفی،  لیستریا اینوکوا به‌ترتیب 128، 256، 256، 256 و 512 میلی‌گرم بر میلی‌لیتر بود و حداقل غلظت کشندگی نانو ذرات نقره برای تمامی باکتری‌ها بزرگتر از 512 میلی‌گرم بر میلی‌لیتر بود. قطر هاله عدم رشد برای باکتری سودوموناس آئروژینوزا (حساس‌ترین سویه) در روش‌های انتشار در دیسک و چاهک آگار در غلظت 150 میلی‌گرم بر میلی‌لیتر به‌ترتیب 13 و 75/7 میلی‌متر بود. در حالی که قطر هاله عدم رشد برای باکتری استافیلوکوکوس اورئوس (مقاوم‌ترین سویه) در روش‌های انتشار در دیسک و چاهک آگار در غلظت 150 میلی‌گرم بر میلی‌لیتر به‌ترتیب 8 و 75/6 میلی‌متر بود. نتایج این پژوهش نشان داد که عصاره برگ گیاه شیشه‌شور قادر به سنتز نانو ذرات نقره می‌باشد و نانو ذرات سنتزی فعالیت ضدمیکروبی مناسبی بر سویه‌های بیماری‌زا در شرایط برون‌تنی از خود نشان داد.

کلیدواژه‌ها

• عصاره آبی
• برگ شیشه‌شور
• نانو ذرات نقره
• باکتری‌های گرم مثبت و گرم منفی

موضوعات

• زیست فناوری مواد غذایی

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