Measurement and Comparison of Nitrate, Nitrite and Toxic Elements in First Harvest and Re-harvest Rice

Document Type : Research Article

Authors

• Zeinab Soltan Touyeh
• Shiva Dehghan Abkenar
• Nazanin Khakipour

Department of Agriculture and Chemistry of Savadkooh Branch, Islamic Azad University, Savadkooh, Iran

https://doi.org/10.22067/ifstrj.2023.75970.1160

Abstract

 
Introduction
 Rice as a staple food, especially in Asian countries, can be a major source of heavy metals. Heavy metals also enter the soils where crops grow naturally and / or through human activities. Metals are absorbed and accumulated in the edible parts of the plant and enter the food chain. Toxic metals, which are present in nature due to industrialization, have polluted the environment, including soil, air, water and food, and have adverse effects on human health through food chains. The Codex Organization has set maximum levels for these elements in various cereals to protect trade and health. Nitrate and nitrite are naturally present in soil, water and food. But today, foods have higher levels of nitrate and nitrite. Excessive use of nitrogen fertilizers to achieve higher yields and improper disposal of human and animal wastes may lead to nitrate accumulation in agricultural products. Very few studies have been performed on the measurement of heavy metal and nitrogen indices in replanted rice. The aim of this study was to measure the amounts of heavy metals (lead, cadmium and arsenic) and the amounts of nitrate and nitrite in first harvest rice and re-harvest rice and compare it with the standard values of the World Health Organization.
 
Methods and Materials
 In this study, a total of 18 rice samples were prepared from three selected farms in the first and second cultivation times and the amount of nitrite, nitrate and heavy metals lead, cadmium, arsenic and mercury were evaluated.
 
Results and Discussion
 The results showed that in all farms in the second crop the concentration of lead decreased significantly (P<0.05). The concentration of lead in all treatments of the first crop Has been more than allowed and in the second cultivation, the treatment of field number one and two, is more than allowed (P<0.05). The concentration of cadmium in all treatments is within the allowable range and in the second crop compared to the first crop of fields number one and three has a significant decrease and in field number two has increased significantly (P <0.05). The highest amount of cadmium is related to field treatments number three. The highest amount of arsenic was observed in the first crop of farm number one and it is more than the allowable limit and in other treatments the amount of arsenic was less than the allowable level and in all three farms the concentration of arsenic in the second crop was significantly reduced compared to the first crop. Regarding mercury, in fields number one and two, with the change of cultivation, the amount of mercury increased significantly and in field number three, there was a significant decrease (P <0.05). Mercury concentration is less than the allowable limit only in the second culture sample of farm number three. Nitrite and nitrate concentrations were also low in all treatments and were considered zero. Experiments showed the amount of nitrite and nitrate in all samples to be negligible and undetectable. Due to the fact that the detection limit of the method (LOQ) used to measure nitrate and nitrite is 100 ppb, the amount of nitrate and nitrite in all samples can be less than 100 ppb. The permissible level of nitrate in food products and rice grains is set at 50 mg/kg according to national standard 16596. The results of the samples showed that all 18 samples had lower amounts of nitrate than the allowable limit. Therefore, rice samples prepared from the first and second crops, their nitrate content is less than the allowable limit and have a complete degree of health.
 
Conclusion
 According to the results obtained, all rice cultivated in the first and second crops have some arsenic, cadmium and lead, but the amount measured in some samples is less and in others, more than specified in the national standard of Iran.  their consumption may be dangerous for consumers. These results also indicate that due to the stability of the field and plant type, there is a positive and significant relationship between the amount of heavy metals studied in rice and the time of cultivation, and this requires further studies on heavy metal contamination in the region. Take place. Therefore, with the conducted studies, it can be concluded that there are concerns in the consumption of rice cultivated in the city of Mazandaran province, in terms of the possibility of endangering the health of consumers.

Keywords

• First harvest rice
• Re-harvest
• Heavy metals
• Nitrate
• Nitrite

Main Subjects

• Food Chemistry

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

References

1. Akbari, Sh., & Cheraghi, M. (2019).Investigation of the concentration of heavy metals zinc, lead and cadmium in rice offered in the consumer market of Hamadan.Journal of Environmental Science and Technology, 21(8), 13-22. https://doi.org/10.22034/jest.2020.16352.3260
2. Amirahmadi, N., Abdpoor, A., Bagheri, A., & Keyvanbehjoo, F. (2015). Study and comparison between farm and rice production using two management systems. 3^th National Congress on Organic and Conventional, Ardebil, Iran. (In Persian)
3. Atefi, M., & Mahmoudzadeh, M. (2021). Determination of nitrate and nitrite in agricultural crops distributed in northeast of Iran. Human, Health and Halal Metrics, 2(2), 18-24. https://www.sid.ir/en/journal/ViewPaper.aspx?id=901033
4. ATSDR, (2003). Agency for Toxic Substances and Disease Registry of the U.S. Department of Health and Human Services. http://www.atsdr.cdc.gov/spl/.
5. Bosque, M.A., Schuhmacher, M., Domino, J.L., & Lobet, J.M. (1990). Concentration of Lead and Cadmium in edible in vegetable from Tarragona province Spain. Science of the Total Environment, 95, 61-67.
6. Bian, Zh., Wang, Y., Zhang, X., Li., T., Grundy, S., Yang, Q., & Cheng, R. (2020). A review of environment effects on nitrate accumulation in leafy vegetables grown in controlled environments. Foods Review.
7. Cao, H., Chen, J., Zhang, J., Zhang, H., Qiao, L., & Men, Y. (2010). Heavy metals in rice and garden vegetables and their risks to inhabitants in the vicinity of an industrial zone in Jiangsu, China. Journal of Environmental Sciences, 22, 1792-1799.
8. Codex Alimentarius, Commission. (2014). Report of the Eighth Session of the Codex Committee on Contaminants in Foods. In Proceedings of the Joint FAO/WHO Food Standards Programme Codex Alimentarius Commission 37th Session, Geneva, Switzerland, December 2014. [Google Scholar]
9. Davis, J.M., Elias, R.W., & Grant, L.D. (1993). Current issues in human lead exposure and regulation of lead. Neurotoxicology, 14, 2–3, 15–27.
10. Deng, F., Yu, M., Martinoia, E., & Song, W.Y. (2019). Ideal cereals with lower arsenic and cadmium by accurately enhancing vacuolar sequestration capacity. Frontiers in Genetics, 10, 322. https://doi.org/10.3389/fgene.2019.00322
11. Farahmandfar, R., Esna Ashari, M., Rashidai Abandansari S., & Maghsoudloo E. (2019). Investigation of mycotoxins and heavy metals in rice samples of Mazandaran province by HPLC and atomic absorption spectroscopy.Iranian Journal of Food Science and Technology, 15(84), 231-242.
12. Institute of Standards and Industrial Research of Iran. Food & Feed-Maximum limit of heavy metals ISIRI. No.12968. Edition, Karaj: ISIRI; 2010. (In Persian)
13. Karbasi, M., Karbasi, E., Saremi, A., & Ghorbani zade Kharazi, H. (2010). Determination of heavy metals concentration in drinking water resources of Aleshtar in 2009. Yafte, 12(1).
14. Khanam, R., Kumar, A., Nayak, A.K., Shahid, Md., Tripathi, R., Vijayakumar, S., Bhaduri, D., Kumar, U., Mohanty, S., & Panneerselvam, P. (2020). Metal(Loid)s (as, Hg, Se, Pb and Cd) in Paddy Soil: Bioavailability and Potential Risk to Human Health. Science of the Total Environment, 699, 134330.
15. Kikuchi, T., Okazaki, M., Toyota, K., Motobayashi, T., & Kato, M. (2007). The input-output balance of cadmium in a paddy field of Tokyo. Chemosphere, 67, 920–927. https://doi.org/10.1016/j.chemosphere.2006.11.018
16. Kim, H.S., Kim, Y.J., & Seo, Y.R. (2015). An overview of carcinogenic heavy metal: molecular toxicity mechanism and prevention. Journal of Cancer Prevention, 20, 232–240. https://doi.org/10.15430/JCP.2015.20.4.232
17. Kolah Kaj, M., Bataleboui, P., Manipour, H.& Modiri, S. (2016). Investigation of arsenic concentration in rice samples and human input dose in Midwood region, Khuzestan.Journal of Health and Environment, Scientific Research Quarterly.
18. Kormoker, T., Porshad, R., Islam, M.D.S, Tusher, T.R., Uddin, M., Khadka, S., Chandra, K. & Sayeed, A. (2021). Presence of toxic metals in rice with human health hazards in Tangail district of Bangladesh. International Journal of Environmental Health Research. https://doi.org/10.1080/09603123.2020.1724271
19. Meharg, A.A., Norton, G., Deacon, C. Williams, P. Adomako, E.E., Price, A., Zhu, Y., Li, G., Zhao, F.J., & McGrath, S. (2013).Variation in rice cadmium related to human exposure. Environmental Science & Technology, 47, 5613–5618.
20. Nies, D.H. (2003). Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiology Reviews, 27, 313–339.
21. Nouri, M. Z., Gholami, M., Mousavi, S.A.A. & Hosseini, S.S. (2014). Study of rice replanting in Mazandaran and comparison of crop indices of rice cultivars in two cultivations. The First International Congress and the Thirteenth National Congress of Agronomy and Plant Breeding. 26-28 August.
22. Nozari, H. (2019). Investigation of cadmium, lead and nitrogen in rice in Abarj Fars region.Iranian Journal of Agriculture and Plant Breeding, 15(1), 15-28.
23. Pirrone, N., Cinnirella, S., Feng, X., Finkelman, R., Friedli, H., Leaner, J., Mason, R., Mukherjee, A., Stracher, G., & Streets, D. (2010). Global mercury emission to the atmosphere from anthropogenic and natural sources. Atmospheric Chemistry and Physics, 10, 5951–5964.
24. Punshon, T., Jackson, B.P., Meharg, A.A., Warczack, T., Scheckel, K., & Guerinot, M.L. (2017). Understanding arsenic dynamics in agronomic systems to predict and preven uptake by crop plants. Science of the Total Environment, 581-582, 209–220.
25. Rezaei, L., Alipour, V., Sharafi, P., Ghaffari, H., Nematollahi, A., Pesarakloo, V., & Fakhri, Y. (2021). Concentration of Cadmium, Arsenic and Lead in rice (Oryza sativa) and probabilistic health risk assessment: A case study in Hormozgan province, Iran. Environmental Health Engineering and Management Journal, 8(2), 67-75. https://doi.org/10.34172/EHEM.2021.10
26. Roberts, T.L. (2014). Cadmium and phosphorous fertilizers: the issues and the science. Procedia Engineering, 83, 52–59.
27. Rothenberg, S.E., Feng, X., Zhou, W., Tu, M., Jin, B., & You, J. (2012). Environment and genotype controls on mercury accumulation in rice (Oryza sativa) cultivated along a contamination gradient in Guizhou. China. Science of the Total Environment, 426(1), 272–280.
28. Selin, N.E. (2009). Global biogeochemical cyclying of mercury: a review. Annual Review pf Environment and Resources, 34, 43.
29. Shirzad, B., & Khakipour, N. (2021). Investigation of heavy metal, lead, cadmium and arsenic contamination in high-consumption Anbarbo rice samples in some areas of Khuzestan province. Journal of Food Science and Nutrition, 19(1), 45-54. https://doi.org/10.30495/jftn.2021.19175
30. Shokrzadeh, M., Rokni, A., & Galstvan, M. (2013). Lead, cadmium and chromium concentration in irrigation supply of Tarom rice in central cities of Mazandaran-Iran. Journal of Mazandaran University of Medical Science, 23(98), 234-242.
31. Sivasinthujah, P., Srikaran, R., Thavaranjit, A.C., Velauthamurty, K., Tharmila, C.J., Abiman, P., & Iyngaran, P. (2014). Contents of nitrate, nitrite and the occurrence of bacteria in fermented cooked parboiled rice and their potential ingestion in the diet. Journal of Microbiology and Biotechnology, 4(4), 56-61.
32. States, J.C. (2015). Arsenic: Exposure Sources, Health Risks, and Mechanisms of Toxicity. John Wiley & Sons, Hoboken, New Jersey (Chapter 2).
33. Tang, W., Dang, F., Evans, D., Zhong, H., & Xiao, L. (2017). Understanding reduced inorganic mercury accumulation in rice following selenium application: selenium application routes, speciation and doses. Chemosphere, 169, 369-376.
34. Tatah Mentan, M., Nyachoti, S., Scott, L., Phan, N., Okwori, F. O., Felemban, N., & Godebo, T.R. (2020). Toxic and essential elements in rice and other grains from the United States and other countries. International Journal of Environmental Research and Public Health, 17(21), 8128. https://doi.org/10.3390/ijerph17218128
35. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K., & Sutton D.J. (2012). Heavy metal toxicity and the environment. Supply Chain Management, 101, 64-133.
36. Vahaji, N., Tayefe, M., & Sadeghi, M. (2020). Comparison of the concentration of heavy elements and their weekly absorption in consumed rice planted in different regions of Guilan province. Ebnesina, 21(4), 51-58.
37. (2004). Joint FAO/WHO Expert Standards. Programme Codex Alimentations Commission. Geneva, Switzerland available in the: http:/www. who. int.
38. Wuana, R.A., & Okieimen, F.E. (2011). Heavy Metals in Contaminated Soils: A Review of Sources, Chemistry, Risks and Best Available Strategies for remediation. International Scholarly Research Notices. https://doi.org/10.5402/2011/402647
39. Zhang, H., Feng, X., Larssen, T., Shang, L., & Li, P. (2010). Bioaccumulation of methylmercury versus inorganic mercury in rice (Oryza sativa) grain. Environmental Science & Technology, 44, 4499–4504.