with the collaboration of Iranian Food Science and Technology Association (IFSTA)

Document Type : Research Article

Authors

Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, Iran.

Abstract

Introduction: Today, the demand for low calorie food based on fruits and easy production and keeping primary features including texture and taste is increasing. Jelly is one of low calorie products which is produced from fruits and other components, and its usage is increasing for human health. Chemical ingredients and natural features of fruits decrease the cancer. In addition to nutrition and medical values, rheological and texture features affect the general quality of fruit jelly. Gelatin is a protein which is obtained by thermal hydrolysis of collagen and is the main protein of bone, cartilage and skin. The source, animal age and the type of collagen are influential factors on gelatin characteristics. Different gelatins have different thermal and rheological characteristics such as transformation temperature to jelly and melting temperature. The melting temperature of gelatin is lower than human body temperature. In food industry, gelatin is used as an alternative for fat, to improve elasticity and transparency of fruit juices and also is used in production of jelly, chocolate, edible films, and so on. Gelatin quality and its application in industry are mainly because of its rheological features. Global demand for gelatin during recent years increases due to its low cost and solubility in biologic environments.

Materials and methods: In this study, the gelatin from chicken feet was extracted by acidic method using hydrochloric acid 0.5% normal with the rate of 1: 3.22 weight / volume. pH was adjusted to 7 by the use of sodium hydroxyl 1 normal and was dried in an incubator at 450 c for 28 hours. Different tests such as pH, protein, ash, moisture, fat, viscosity, jelly strength, color and rheology were done in gelatin molecule to measure of storage modules (G') and loss modules (G''). Then, the effect of extracted jelly in concentrations of 0 to 1.5 % on the physico-chemical (Brix, humidity, acidity, color, texture) and organoleptic properties of cantaloupe jelly (odor, sweetness, color, appearance, jelly status, transparency, adhesion) were investigated using five point Hedonic scale ranked. Experiments related to cantaloupe jelly were conducted in terms of a completely random design. A one-way analysis of variance and Duncan test (P≤ 0.05), in three replications were used to establish the significance of differences in experimental data’s. The result was performed using the SPSS version 16.0 windows program, and charts were plotted with Excel 2010.

Results and Discussion: The results showed that the average yield of gelatin based on the wet weight was 4.80%, pH value before drying is 3.7, the total amount of protein is 83.95%, the total amount of ash is 0.89%, moisture is 9.66%, fat is 0%, viscosity is 216 centipoise. The strength of jelly is 487g that in compare with other alternatives such as gelatin from chicken skin is 355±48.1 gr, cow gelatin is 299±71.1 gr, fish gelatin including 181 to 263 gr for tilapia, 280 gr for horse mackerel fish, 125 and 177 gram for Sin croaker and Shortfin scad, respectively. The main reason for low level of gelatin in fish skin is the low amount of hydroxyl proline. Thus, it can be claimed that the high gel strength in chicken feet gelatin might be due to lower extraction temperature, strong hydrogen bond and more probably hydroxyl proline. Hydrocolloid solutions are usually viscoelastic; therefore, the level of storage modules (G') and loss modules (G'') is measurable for them. Gelatin from chicken feet exhibited G' greater than the G'' in a wide range of frequencies of the oscillatory test, which have indicated the gel network is high stable. By increasing the amount of gelatin in the cantaloupe juice, transparency and firmness of cantaloupe jelly increased. In the sensory analysis, jellies had not significant differences in the intensity of sweetness. In terms of overall acceptability, the sample containing 1.25 and 1.5 percent of gelatin gained maximum score.

Keywords

استاندارد ملی ایران، 1392، فرآورده های ژله ای -ویژگی ها و روش های آزمون، اصلاحیه شماره1، شماره 2682.
Almeida, P., Silva Lannes, S., Calarge, F., Brito Farias, T . and Curvelo Santana, J. 2012. FTIR characterization of gelatin from chicken feet. Journal of chemistry and chemical engineering. 6(11): 1029-1032.
AOAC. 2006. Official methods of analysis of AOAC international (18th ed.). Virginia,USA: Association of official and analytical chemists international.
Badii, F., and Howell, N. K. 2006. Fish gelatin: structure, gelling properties and
interaction with egg albumen proteins. Food hydrocolloids. 20, 630-640.
Benali, S., Benamara, S., Bigan, M. and Madani, Kh. 2014. Feasibility study of date (Phoenix dactylifera L.) fruit syrup-based natural jelly using central composite design. J. Food sci. technol. DOI. 10. 1007/s13197-014-1529-x.
Binsi, P.K., Shamasundar, B.A., Dileep, A.O., Badii, F. and Howell, N.K., 2009. Rheological and functional properties of gelatin from the skin of Bigeye snapper (Priacanthus hamrur) fish: Influence of gelatin on the gel-forming ability of fish mince. Food hydrocolloids. 23, 132–145.
Boran,G and Joe., Regenstein,M. 2010. Fish gelatin. Advances in food and nutrition research. DOI. 10.1016, s1043-4526 (10) 60005-8.
Chandra, M.V. and Shamasundar, B.A. 2015. Rheological properties of gelatin prepared from the swim bladders of freshwater fish Catla catla. Food hydrocolloids. 48, 47- 54.
Cheow, C. S., Norizah, M. S., Kyaw, Z. Y. and Howell, N. K. 2007. Preparation and characterisation of gelatins from the skins of sin croaker (Johnius dussumieri) and shortfin scad (Decapterus macrosoma). Food chemistry. 101, 386-391.
Gaspar, C., Laureano, O.and Sousa, I., 1998. Production of reduced-calorie grape juice jelly with gellan, xanthan and locust bean gums: sensory and objective analysis of texture. European food research and technology/ Z lebensm unters Forsch A .206, 169-174.
Gomez-Guillen, M.C., Gimenez, B., Lopez-Caballero, M.E. and Montero, M.P. 2011. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food hydrocolloids. 25, 1813-1827.
Grossman, S., and Bergman, M. 1992. Process for the production of gelatin from the fish skins. US Patent . 5,093,474.
Hanani,Z.A., Roos,Y.H. and Kerry,J. 2012. Use of beef, pork and fish gelatin sources in the manufacture of films and assessment of their composition and mechanical properties. Food hydrocolloids. 29,144-151.
Jamilah, B., and Harvinder, K. G. 2002. Properties of gelatins from skins of fish-black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food chemistry. 77, 81-84.
Karim, A.A. and Bhat, R. 2009. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food hydrocolloids. 23, 563–576.
Kasankala, L. M., Xue, Y., Weilong, Y., Hong, D.S. and He, Q. 2007. Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology. Bioresource technology. 98, 3338–3343.
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F., 2010. Comparative study on characteristics of gelatin from the skins of brownbanded bamboo shark and blacktip shark as affected by extraction conditions. Food hydrocolloids. 24, 164–171.
Lassoued,I., Jridi,M., Nasri,R., Dammak,A., Hajji,M., Nasri,M. and Barkia, Ahmed. 2014. Characteristics and functional properties of gelatin from thornback ray skin obtained by pepsin-aided process in comparison with commercial halal bovine gelatin. Food hydrocolloids. 41, 309-318.
Lee, H.E., Yeom, J., Ha, S.M. and Bae, H.D. 2010. Development of Banana Peel Jelly and its antioxidant and textural properties. Food sci. biotechnol. 19(2): 449-455.
Norziah, M.H., Al-Hasan, A., Khairulnizam, A.B., Mordi, M.N. and Norita, M. 2009. Characterization of fish gelatin from surimi processing wastes: Thermal analysis and effect of transglutaminase on gel properties. Food hydrocolloids. 23, 1610-1616.
Sarbon,N., Abdi,F. and Howell,N. 2013. Preparation and characterisation of chicken skin gelatin as an alternative to mammalian gelatin. Food hydrocolloids. 30, 143-151.
Shakila,R., Jeevithan, E., Varatharajakumar, A., Jeyasekaran,G. and Sukumar, D. 2012. Functional characterization of gelatin extracted from bones of red snapper and grouper in comparison with mammalian gelatin. LWT - Food science and technology. 48, 30-36.
Shyni, K., Hema, G.S., Ninan, G., Mathew, S., Joshy, C.G. and Lakshmanan, P.T.2014. Isolation and characterization of gelatin from the skins of skipjack tuna (Katsuwonus pelamis), dog shark (Scoliodon sorrakowah), and rohu (Labeo rohita). Food hydrocolloids. 39, 68-76.
Sousa, N.M., Matias, C. and Laureano, O.1997. The texture of low calorie grape juice jelly. European food research and technology/ Z Lebensm unters forsch A. 205, 140–142.
Trachootham, D., Songkaew, W., Hongsachum, B., Wattana, Ch., Changkluengdee, N., Karapoch, J., Thirdsuttironnapumi, S., Meennuch, E., Klaitong, Ch., Sinthusek, T. and Lam-u bol, A. 2015. Nutri-jelly may improve quality of life and decrease tube feeding demand in head and neck cancer patients, Support care cancer. 23, 1421–1430
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