with the collaboration of Iranian Food Science and Technology Association (IFSTA)

Document Type : Research Article

Authors

, Department of Food Science and Technology, Faculty of Agriculture, Islamic Azad University, Isfahan branch (Khorasgan), Isfahan, Iran

Abstract

[1]Introduction: Nowadays, various methods have been developed to transfer and improve the absorption of lipophilic compounds in food in the form of coating. Colloidal emulsion-based systems are widely used in commercial systems. Chia seed oil is an oil rich in unsaturated fatty acids, especially omega 3 and omega 6, which can be used as the oily phase of nanoemulsions. The aim of this study was to investigate the fatty acid composition of chia seed oil and the effect of oil to water ratio and emulsifier on the properties of nanoemulsions of this oil. Materials and Methods: Clean chia seeds were purchased as a pack from Zistfa Company and all other chemicals were purchased from Marack, Pars Shimi and Dr. Majelli companies. By using the oil set machine, the seeds were treated by cold pressing method and oil extraction efficiency was calculated .Gas chromatography (GC) was used to identify and measure the fatty acid composition of chia seed oil. In this study, nanoemulsions were produced in three levels of oil to water (20%, 35% and 50%) and in three levels of oil emulsifier (5%, 10% and 15%).To produce nanoemulsions, the aforementioned ratios were first calculated and determined .Then, Weigh the tweens 20 and 80 with chia seed oil and water and mix the emulsifiers with the chia seed oil on the stirrer. Then a mixture of 20 and 80 tweens and oil was added dropwise to the weighed distilled water. The mixture was stirred gently by a magnet. After this step, the mixture is placed in the refrigerator to reduce its temperature. After this time, it was placed in an ice bucket and placed in an ultrasonic homogenizer for 9 minutes at a power of 300 watts to form nanoemulsions. Then, the properties of nanoemulsions including particle size and distribution, coating efficiency and antioxidant properties were evaluated. Vasco model DLS (Dynamic light scattering) was used for the particle size. The particle size of nanoemulsions was determined by this device using dynamic light diffraction method. DPPH method was used to measure the antioxidant activity of oils and nanoemulsions and the antioxidant activity was calculated by using the formula. To evaluate the efficiency of coating of nanoemulsions, the amount of surface oil and total oil was measured and the efficiency was calculated by using the formula. The release rate of nanoemulsions was evaluated for 7 weeks. The tests were performed in three replications. Experimental data were analyzed in a completely randomized design with factorial test and Duncan test was used to compare the mean data. Software (Excel, 2010) was used to draw the graphs Results and Discussion: According to the results obtained in this study, it can be said that chia seed oil contains a high percentage of unsaturated fatty acids and has a very high level of antioxidant properties (88.43%), which has antioxidant properties in the structure of nanoemulsions. Oil is better preserved in water. Other results showed that with increasing oil content of nanoemulsions, particle size, antioxidant properties and release increased and the coating efficiency decreased. Also, by increasing the ratio of emulsifier to oil in nanoemulsions, the coating efficiency and antioxidant properties increase and the particle size and release decreases. In the results obtained from these tests, the minimum particle size was 14.08 nm and the highest coating efficiency was 96.40%. The antioxidant activity of the samples was evaluated in the range of 5.61% to 21.43%. Also, the average release of samples at the end of 7 weeks of the 
study reached nearly 18%. During storage, the treatments were quite stable for five months, which could be due to the low particle size and low release of samples. Considering the mentioned advantages, using nanoemulsions of this oil is a suitable option for enriching beverages. 

Keywords

  1. Abdollahi, A., ShafafiZenozian, M., Saeedi Asl, M., Armin, M., Big Babaei, A. (2018). 'Investigation of the effect of microwave pretreatment on physicochemical properties of sesame seed oil', Innovation in Food Science and Technology, 10 (2), pp. 13-21.
  2. Akinfenwa, A. O., Cheikhyoussef, A., Cheikhyoussef, N. Hussein, A. A., (2020). Cold pressed chia (Salvia hispanica) seed oil. In Cold Pressed Oils (pp. 181- 190). Academic Press. https://doi.org/10.1016/B978-0-12-818188-1.00015-3
  3. Anacleto, S., Ruiz, G., Rana, J., Gordillo, G., West, H., Sharma, M., et al. (2016). Chia crop (Salvia hispanica): Its history and importance as a source of polyunsaturated fatty acids omega-3 around the world: A review. Journal of Crop Research and Fertilizers, 1(104), 1–9
  4. Assadpour, E., Maghsoudlou, Y., Jafari, S. M., Ghorbani, M., Aalami, M., (2016). Optimization offolic acid nano-emulsification and encapsulation by maltodextrin-whey protein double emulsions. J. Biol. Macromol. 86, 197–207.
  5. Ayerza, R., & Coates, W. (2011). Protein content, oil content and fatty acid profiles as potential criteria to determine the origin of commercially grown chia (Salvia hispanica). Industrial Crops and Products, (34), 1366– 1371. https://doi.org/10.1016/j.indcrop.2010.12.007
  6. Bushway, A., Belyea, P. R., & Bushway, R. J. (1981).Chia Seed as a Source of oil, polysaccharide, and protein. Journal of Food Science, (46), 1349- 1350. https://doi.org/10.1111/j.1365-2621.1981.tb04171.x
  7. Chahardoli, M., Ahmadichenarbon, H .(2017). Evaluation of nutritional value of chia seeds (Hispanica Salvia L). First International Conference on Applied Research in Agricultural Sciences, Natural Resources and Environment, https://civilica.com/doc/674104
  8. Chen, H., Guan, Y., Zhong, Q. (2015). Microemulsions based on a sunflower lecithin–Tween 20 blend have high capacity for dissolving peppermint oil and stabilizing coenzyme Q10. Journal of Agricultural and Food Chemistry 63(3), 983-989. https://doi.org/10.1021/jf504146t
  9. Faraji, N., Alizadeh, M., Almasi, H., Pirsa, S., Faraji, S. (2020). Optimization of Iranian mentha longifolia of nanoemulsion containing omega-3 fatty acids by spontaneous emulsification, Iranian Food Science and Technology Research Journal, 17(1), 143-172. 
  10. Frascareli, E. C., Silva, V. M., Tonon, R. V., Hubinger, M. D. (2012). Effect of process conditions on the microencapsulationof coffee oil by spray drying. Food and bioproducts processing, 90(3), 413- 424. https://doi.org/10.1016/j.fbp.2011.12.002
  11. Gharenaghadeh, s., Samadlouie, H. R., Sowtim., Hamisekarh., Rezaei Mokaram, R., (2017). Evaluation of the antimicrobial and antioxidant properties of Salvia essential oil nanoliposome (Salvia multicaulis), Food Science and Technology, 14(1), 271-282. magiran.com/p1665862
  12. Ghosh, A., Mandal, A. K., Sarkar, S., Panda, S. Das, N. (2009). Nanoencapsulation of quercetin enhances its dietary efficacy in combating arsenic-inducedoxidative damage in liver and brain of rats. Life Sciences, 84: 75- 80. https://doi.org/10.1016/j.lfs.2008.11.001
  13. Hadian, J., Kohzadi, B., Asadi, M., ShafieZargar, A. R., Ghorbanpour, M. (2019). Evaluation of growth, yield characteristics and oil components of two Chia (Salvia hispanica) varieties in different planting dates, Journal of Medicinal Plants, 18(70), 59- 72. DOI: 10.29252/jmp.2.70.59
  14. Hernandez, L. M. (2012). Mucilage from chia seeds (Salvia hispanica): Microestructure, physico-chemical characterization and applications in food industry (Doctoral dissertation, Pontificia Universidad Catolica De Chile).‏
  15. Iglesias-Puig, E. and Haros, M., (2013). Evaluation of performance of dough and bread incorporatingchia (Salvia hispanica). European Food Research and Technology, vol. 237, 2013, pp.1- 10. https://doi.org/10.1007/s00217-013-2067-x
  16. Imani Motlagh, Y., Gharachorloo, M. (2020). Chemical evaluation of oil extracted from chia Seed (Salvia hispanica), Journal of Food Technology & Nutrition, 18(1), 27-36. 
  17. Iranian Institute of Standards and Industrial Research., 2004, Analysis of fatty acid methyl esters by gas chromatography. Iranian National Standard No. 4091
  18. Ixtaina, V. Y., Martínez, M. L., Spotorno, V., Mateo, C. M., Maestri, D. M., Diehl, B. W., Nolasco, S. M. and Tomás, M. C., (2011). Characterization of chia seed oils obtained by pressing and solvent extraction. Journal of Food Composition and Analysis, 24 (2), pp.166- 174. https://doi.org/10.1016/j.jfca.2010.08.006
  19. Jafari, S. M., Assadpoor, E., He, Y. Bhandari, B., (2008). Re-coalescence of emulsion droplets during high-energy Food hydrocolloids, 22 (7), pp.1191- 1202. https://doi.org/10.1016/j.foodhyd.2007.09.006
  20. Kaya, E. C. (2019). Formulation and characterization of chia seed oil nanoemulsions (Doctoral dissertation, Middle East Technical University).‏
  21. Kiralan, S. S., Doğu-Baykut, E., Kittipongpittaya, K., McClements, D. J. and Decker, E. A., (2014). Increased antioxidant efficacy of tocopherols by surfactant solubilization in oil-in-water emulsions. Journal of agricultural and food chemistry, 62 (43), pp.10561- 10566.
  22. Marsanasco, M., Márquez, A. L., Wagner, J. R., Alonso, S. D. V., Chiaramoni, N. S. (2011). Liposomes as vehicles for vitamins E and C: An alternative to fortify orange juice and offer vitamin C protection after heat treatment. Food research international, 44(9): 3039- 3046. https://doi.org/10.1016/j.foodres.2011.07.025
  23. Martínez, M. L., Marín, M. A., Faller, C. M. S., Revol, J., Penci, M. C., &Ribotta, P. D. (2012). Chia (Salvia hispanica) oil extraction: study of processing parameters. LWT- Food Science and Technology, 47(1), 78- 82.‏ https://doi.org/10.1016/j.lwt.2011.12.032
  24. McClements, D, J. and Rao, J. (2011). Food-Grade Nanoemulsions: Formulation, Fabrication, Properties, Performance, Biological Fate, and Potential Toxicity. Critical Reviews in Food Science and Nutrition, 51: 285- 330. https://doi.org/10.1080/10408398.2011.559558
  25. NutraLease (2011). Available from: http://www.nutralease.com/Nutra/ Templates/ showpage. asp ?DBID= 1&LNGID= 1&TMID= 84&FID= 767
  26. Rachmadi W., Permatasari. D, Rahma. A., Rachmawati. H., (2015). Self- nanoemulsion containing combination of curcumin and silymarin: Formulation and characterization. Research and Development on Nanotechnology in Indonesia 2(1): 37- 48.
  27. Ribeiro, R. C. D. A., Barreto, S. M. A. G., Ostrosky, E. A., Rocha-Filho, P. A. D., Veríssimo, L. M., and Ferrari, M. (2015). Production and characterization of cosmetic nanoemulsions containing Opuntia ficus-indica (L.) mill extract as moisturizing agent. Molecules, 20 (2), 2492- 2509. https://doi.org/10.3390/molecules20022492
  28. Saberi, A. H., Fang, Y. and McClements, D. J., (2013). Fabrication of vitamin E-enriched nanoemulsions: factors affecting particle size using spontaneous emulsification. Journal of colloid and interface science, 391, pp.95- 102. https://doi.org/10.1016/j.jcis.2012.08.069
  29. Tavakolipour, H., Mokhtarian M, (2016). Nano-encapsulation of pomegranate seed oil by liquid- liquid dispersion method and oil releasing in gastric simulated conditions, Iranian Journal of Nutrition Sciences & Food Technology, 11(2), 75-84. magiran.com/p1559678
  30. Timilsena, Y. P., Vongsvivut, J., Adhikaria, R. & Adhikari, B. (2017). Physicochemical and thermal characteristics of Australian chia seed oil. Food Chemistry, (228), 394– 402. https://doi.org/10.1016/j.foodchem.2017.02.021
  31. Uluata, S., McClements, D. J. Decker, E. A. (2015). Physical stability, autoxidation, and photosensitized oxidation of ω-3 oils in nanoemulsions prepared with natural and synthetic surfactants. Journal of agricultural and food chemistry, 63(42), 9333- 9340. https://doi.org/10.1021/acs.jafc.5b03572
  32. Velasco, L. Fernandez- Martinez, J. M. (2002).Breeding oilseed crops for improved oil Journal of Crop Production5(1-2), 309-344. https://doi.org/10.1300/J144v05n01_13
  33. Venkadesaperumal, G., Rucha, S., Sundar, K., and Shetty, P. H. (2016). Anti-quorum sensing activity of spice oil nanoemulsions against food borne pathogens. LWT- Food Science and Technology, 66, 225- 231. https://doi.org/10.1016/j.lwt.2015.10.044
  34. Wilkinson, J. B., (1994). Harry'sCosmeticology. 7 ed: Longman Scientific and Technical.
  35. Wooster, T. J., Golding, M. and Sanguansri, P., (2008). Impact of oil type on nanoemulsion formation and ostwald ripening stability. Langmuir, 24(22), pp.12758- 12765. https://doi.org/10.1021/la801685v

 

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