Production of Whey Protein Concentrate Smart Film Containing Pomegranate and Red Grape Anthocyanin and Zinc Oxide Nanoparticles: Physicochemical and Structural Characteristics

Document Type : Research Article

Authors

• Ebrahim Taghizadeh ¹
• Mohammad Alizadeh khaled abad ¹
• Hamed Hassanzadeh ²

¹ Department of Food Science and Technology, Urmia University, Urmia, Iran

² Department of Food Science and Hyeigene, Ilam University, Ilam, Iran

https://doi.org/10.22067/ifstrj.2024.84940.1289

Abstract

Introduction
Edible films and coatings are suitable for fresh, semi-processed foods, and also for processed fruits and vegetables because they can increase their shelf life, protect them from the activity of microorganisms, and ultimately improve their nutritional and sensory value. In addition, edible films and coatings are able to transfer compounds that may have been added to improve the performance of the coating film and better preserve the product or have benefits for the consumer. The present research was conducted with the aim of investigating the structural physicochemical properties of whey protein concentrate smart film containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles.
 
Material and Method
To prepare nano composite film, 10 grams of whey protein concentrate powder was added to 90 ml of distilled water. At the beginning of the production of the film, the pH was adjusted to pH = 8 with the help of 0.1 normal soda, and in order to better dissolve the isolate, the solution was heated for 30 minutes at 80 degrees Celsius to ensure denaturation. It was then placed on a magnetic stirrer. After cooling the solution, pomegranate and red grape anthocyanins were added to the solution separately according to the statistical plan. The solution was then  homogenized for 10 minutes at 13,000 rpm, and finally zinc oxide nanoparticles were added to the solution according to the statistical plan and homogenized on a magnetic stirrer for 10 minutes, and then in an ultrasound bath to disperse for 10 minutes In the next step, glycerol was added to the 40% of the weight of the dry substance and placed on a rotating magnetic stirrer without heat for 15 minutes. The formed solution was aerated for 10 minutes and 25 ml of it was poured in the center of the plate (8 cm). Then the film was dried in the oven at 38 degrees Celsius and the control film was produced. After preparing the films, the color of the film samples was determined by measuring the color components L* (lightness), a* (red/green) and b* (yellow/blue) using a Hanterlab colorimeter. The thickness of the films was measured by a digital micrometer with an accuracy of 0.001 mm at 10 random points of each film. Finally, humidity, solubility and FTIR measurement tests were also performed for the prepared films. The preparation of whey protein concentrate smart composite film was investigated based on two variables: red grape anthocyanin/pomegranate anthocyanin percentage and copper oxide nanoparticle percentage. Each of which was investigated in five levels and the response surface methodology (RSM) of the central composite design was used. A total of 24 composite films were prepared and their physical and chemical properties were investigated. Also, after analyzing the data, the optimal movies were checked based on optimization and utility function by 11 Design expert statistical software.
 
Results and Discussion
By increasing the amount of anthocyanin and zinc oxide nanoparticles, the thickness of the layers increased up to the middle point (0.58-0.89 mm), and the largest increase in thickness was observed in samples with 1.5 cc of anthocyanin and 2% of zinc oxide nanoparticles. Also, films with low concentrations of anthocyanin (0.1-4 cc) and a high percentage of the selected range of zinc oxide (0.2-0.7%) show the highest percentage of solubility. The added zinc oxide nanoparticles increased the solubility of the films. FTIR analysis showed that the interactions between whey concentrate and anthocyanins were probably responsible for the changes in the properties of the composite layers. The utility function for each response was estimated by numerical methods and the general utility function was obtained for the optimal samples of red grape and pomegranate. For the optimal pomegranate, the obtained results include the amount of anthocyanin equal to 2.6 cc, zinc oxide nanoparticles 0.6 (weight/volume), solubility 65.37, thickness 0.64 mm, indices a,  L and bwere. 27.88 0.24 and -2.01, respectively. Finally its total desirability has been reported as 0.58. The utility function for each answer was estimated by numerical methods and the general utility function was obtained for the optimal samples of red grapes such as the optimal pomegranate. For the optimal grape film samples, the obtained results include: the amount of anthocyanin equal to 1.38 cc, 3.4 zinc oxide nanoparticles (weight/volume), solubility 59.83%, thickness 0.83 mm, a, L and b indices were 27.03, 7.05 and 1.92 respectively and, its total desirability was 0.63.

Keywords

• Edible film
• Grape anthocyanin
• Pomegranate anthocyanin
• Whey protein concentrate
• Zinc oxide nanoparticles

Main Subjects

• Food Technology

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

References

1. Abdellatif, A.A., Alawadh, S.H., Bouazzaoui, A., Alhowail, A.H., & Mohammed, H.A. (2020). Anthocyanins rich pomegranate cream as a topical formulation with anti-aging activity. Journal of Dermatological Treatment, 1-8. https://doi.org/10.1080/09546634.2020.1721418
2. Abdollahi, M., Rezaei, M., & Farzi, G. (2012). Improvement of active chitosan film properties with rosemary essential oil for food packaging. International Journal of Food Science and Technology, 47, 847-853. https://doi.org/10.1111/j.1365-2621.2011.02917.x
3. Ahmed, T., Shahid, M., Azeem, F., Rasul, I., Shah, A.A., Noman, M., & Muhammad, S. (2018). Biodegradation of plastics: Current scenario and future prospects for environmental safety. Environmental Science and Pollution Research, 25(8), 7287-7298. https://link.springer.com/article/10.1007/S11356-018-1234-9
4. Amit, S.K., Uddin, M.M., Rahman, R., Islam, S.R., & Khan, M.S. (2017). A review on mechanisms and commercial aspects of food preservation and processing. Agriculture & Food Security, 6(1), 1-22. https://link.springer.com/article/10.1186/s40066-017-0130-8
5. Dasgupta, N., Ranjan, S., & Gandhi, M. (2019). Nanoemulsions in food: market demand. Environmental Chemistry Letters, 17(2), 1003-1009. https://link.springer.com/article/10.1007/s10311-019-00856-2
6. Diblan, S., Erdem, B.G., & Kaya, S. (2020). Sorption, diffusivity, permeability and mechanical properties of chitosan, potassium sorbate, or nisin incorporated active polymer films. Journal of Food Science and Technology, 57(10), 3708-3719. https://link.springer.com/article/10.1007/s13197-020-04403-8
7. Díaz‐ García, M.C., Castellar, M.R., Obón, J.M., Obón, C., Alcaraz, F., & Rivera, D. (2015). Production of an anthocyanin‐ rich food colourant from Thymus moroderi and its application in foods. Journal of the Science of Food and Agriculture, 95(6), 1283-1293. https://doi.org/10.1002/jsfa.6821
8. Fathiraja, P., Gopalrajan, S., Karunanithi, M., Nagarajan, M., Obaiah, M.C., Durairaj, S., & Neethirajan, N. (2021). Response surface methodology model to optimize concentration of agar, alginate and carrageenan for the improved properties of biopolymer film. Polymer Bulletin, 1-27. https://link.springer.com/article/10.1007/s00289-021-03797-5
9. Hanani, Z.N., Yee, F.C., & Nor-Khaizura, M.A.R. (2019). Effect of pomegranate (Punica granatum) peel powder on the antioxidant and antimicrobial properties of fish gelatin films as active packaging. Food Hydrocolloids, 89, 253-259. https://doi.org/10.1016/j.foodhyd.2018.10.007
10. Hosseini, M., Hassanzadeh, H., & Miri, P. (2023). Investigating the physicochemical and mechanical properties of edible active films based on gelatin containing essential oils of thyme, oregano and sage. Journal of Food Processing and Preservation. https://doi.org/10.22069/FPPJ.2023.21472.1768
11. Ifmalinda, I., Kurnia, S.A., & Cherie, D. (2023). Characteristics of edible film from corn starch (Zea mays) with additional glycerol and variations of zinc oxide (ZnO) nanoparticles. Journal of Applied Agricultural Science and Technology, 7(3), 272-285. https://doi.org/10.55043/jaast.v7i3.87
12. Jafari, S.M., Ghalenoei, M.G., & Dehnad, D. (2017). Influence of spray drying on water solubility index, apparent density, and anthocyanin content of pomegranate juice powder. Powder Technology, 311, 59-65. https://doi.org/10.1016/j.powtec.2017.01.070
13. Jahed, E., Khaledabad, M.A., Almasi, H., & Hasanzadeh, R. (2017). Physicochemical properties of Carum copticum EO loaded chitosan films containing organic nanoreinforcements. Carbohydrate Polymers, 164, 325-338. https://doi.org/10.1016/j.carbpol.2017.02.022
14. Koehler, J., Wallmeyer, L., Hedtrich, S., Goepferich, A.M., & Brandl, F.P. (2017). pH‐modulating poly (ethylene glycol)/alginate hydrogel dressings for the treatment of chronic wounds. Macromolecular bioscience, 17(5), 1600369. https://doi.org/10.1002/mabi.201600369
15. Li, Y., Jiang, Y., Liu, F., Ren, F., Zhao, G., & Leng, X. (2011). Fabrication and characterization of TiO2/whey protein isolate nanocomposite film. Food Hydrocolloids, 25(5), 1098-1104. https://doi.org/10.1016/j.foodhyd.2010.10.006
16. Liu, C. , Jin, T. , Liu, W. , Hao, W. , Yan, L. , & Zheng, L. (2021). Effects of hydroxyethyl cellulose and sodium alginate edible coating containing asparagus waste extract on postharvest quality of strawberry fruit. LWT, 111770. https://doi.org/10.1016/j.lwt.2021.111770
17. Matta Fakhouri, F., Nogueira, G.F., de Oliveira, R.A., & Velasco, J.I. (2019). Bioactive edible films based on arrowroot starch incorporated with cranberry powder: Microstructure, thermal properties, ascorbic acid content and sensory analysis. Polymers, 11(10), 1650. https://doi.org/10.3390/polym11101650
18. Mileriene, J., Serniene, L., Henriques, M., Gomes, D., Pereira, C., Kondrotiene, K., & Malakauskas, M. (2021). Effect of liquid whey protein concentrate–based edible coating enriched with cinnamon carbon dioxide extract on the quality and shelf life of Eastern European curd cheese. Journal of Dairy Science, 104(2), 1504-1517. https://doi.org/10.3168/jds.2020-18732
19. Misra, S., Pandey, P., & Mishra, H.N. (2021). Novel approaches for co-encapsulation of probiotic bacteria with bioactive compounds, their health benefits and functional food product development: A review. Trends in Food Science & Technology, 109, 340-351. https://doi.org/10.1016/j.tifs.2021.01.039
20. Moghadam, M., Salami, M., Mohammadian, M., & Emam-Djomeh, Z. (2021). Development and characterization of ph-sensitive and antioxidant edible films based on mung bean protein enriched with echium amoenum anthocyanins. Journal of Food Measurement, 15(4), 2984-2994. https://link.springer.com/article/10.1007/s11694-021-00872-3
21. Momeni, R., Hosseini, M., Hassanzadeh, H., & Saifi, T. (2024). production of antimicrobial active edible film based on gelatin containing Salvia officinalis essential oil: physical, mechanical, antioxidant and antimicrobial properties. Food Research Journal, 33(4), 29-43. https://doi.org/10.22034/FR.2023.56857.1877
22. Musso, Y.S., Salgado, P.R., & Mauri, A.N. (2019). Smart gelatin films prepared using red cabbage (Brassica oleracea) extracts as solvent. Food Hydrocolloids, 89, 674-681. https://doi.org/10.1016/j.foodhyd.2018.11.036
23. Nasiri, M., & Tamdan, M, (2014). Investigating the antimicrobial properties of zinc oxide nanoparticles synthesized with the help of ultrasonic waves. Yazd Health Dawn, 13(4), 115-128.
24. Otoni, C.G., Avena‐Bustillos, R.J., Azeredo, H.M., Lorevice, M.V., Moura, M.R., Mattoso, L.H., & McHugh, T.H. (2017). Recent advances on edible films based on fruits and vegetables—a review. Comprehensive Reviews in Food Science and Food Safety, 16(5), 1151-1169. https://doi.org/10.1111/1541-4337.12281
25. Pala, Ç.U., & Toklucu, A.K. (2011). Effect of UV-C light on anthocyanin content and other quality parameters of pomegranate juice. Journal of Food Composition and Analysis, 24(6), 790-795. https://doi.org/10.1016/j.jfca.2011.01.003
26. Panche, A., Diwan, A., & Chandra, S. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5. https://doi.org/10.1017/jns.2016.41
27. Pino, P., Ronchetti, S., Mollea, C., Sangermano, M., Onida, B., & Bosco, F. (2021). Whey proteins–zinc oxide bionanocomposite as antibacterial films. Pharmaceutics, 13(9), 1426. https://doi.org/10.3390/pharmaceutics13091426
28. Razmjoo, F., Sadeghi, E., Rouhi, M., Mohammadi, R., Noroozi, R., & Safajoo, S. (2021). Polyvinyl alcohol–Zedo gum edible film: Physical, mechanical and thermal properties. Journal of Applied Polymer Science, 138(8), 49875. https://doi.org/10.1002/app.49875
29. Rhim, J.-W., Hong, S.-I., Park, H.-M., Ng, P.K.J.J., & Chemistry, F. (2006). Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity. 54(16), 5814-5822.
30. Rodriguez-Amaya, D.B. (2019). Update on natural food pigments-A mini-review on carotenoids, anthocyanins, and betalains. Food Research International, 124, 200-205. https://doi.org/10.1016/j.foodres.2018.05.028
31. Saedi, S., Shokri, M., Kim, J.T., & Shin, G.H. (2021). Semi-transparent regenerated cellulose/ZnONP nanocomposite film as a potential antimicrobial food packaging material. Journal of Food Engineering, 307, 110665. https://doi.org/10.1016/j.jfoodeng.2021.110665
32. Solano Doblado, L. G., Heredia Mira, F. J., Gordillo Arrobas, B., Dávila Ortiz, G., Alamilla Beltrán, L., Maciel Cerda, A., & Jiménez Martínez, C. (2020). pH-indicating properties and storage stability of a smart edible film based on nopal-mucilage/gellan gum and red cabbage anthocyanins. Revista Mexicana de Ingeniería Química, 19(Sup. 1), 363-374. https://doi.org/10.24275/rmiq/Alim1583
33. Taghizadeh, E., Alizadeh, M., & Hassanzadeh, H. (2023). Investigation of antimicrobial, antioxidant and physicochemical properties of active film based on whey protein containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles. Innovative Food Technologies, 10(4), 397-410. https://doi.org/10.22104/IFT.2023.6374.2145
34. Thulasisingh, A., Kumar, K., Yamunadevi, B., Poojitha, N., SuhailMadharHanif, S., & Kannaiyan, S. (2021). Biodegradable packaging materials. Polymer Bulletin, 1-30. https://link.springer.com/article/10.1007/s00289-021-03767-x
35. Velásquez, P., Bustos, D., Montenegro, G., & Giordano, A.J.M. (2021). Ultrasound-assisted extraction of anthocyanins using natural deep eutectic solvents and their incorporation in edible films. Molecules, 26(4), 984. https://doi.org/10.3390/molecules26040984
36. Wang, N., Liu, W., Zhang, T., Jiang, S., Xu, H., Wang, Y., & Chen, X. (2018). Transcriptomic analysis of red-fleshed apples reveals the novel role of mdwrky11 in flavonoid and anthocyanin biosynthesis. Journal of Agricultural and Food Chemistry, 66(27), 7076-7086. https://doi.org/10.1021/acs.jafc.8b01273