with the collaboration of Iranian Food Science and Technology Association (IFSTA)

Document Type : Research Article

Authors

Faculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

Introduction: The edible films and coatings had remarkable growth in recent years to increase the shelf life and to enhance food quality, stability, and safety and expected to have an important impact on the food market in the following years. In addition, these matrices can be used as carriers of antimicrobials to minimize the risk of foodborne contamination by pathogens and inhibit the development of spoiler microbes. Antimicrobial packaging is a type of active packaging that provides the continuous migration of antimicrobial components to the surface of the foods. Chitosan is a linear copolymer of β-1, 4-linked D-glucosamine and N-acetyl-d-glucosamine. It is a cationic polysaccharide for food packaging applications, due to its unique characteristics of films, including excellent oxygen barrier properties, good mechanical properties, nontoxicity and good antimicrobial activity. Eucalyptus is a plant native from Australia and the Myrtasya family that includes about 900 species and sub-species. There is abundant scientific evidence regarding the efficacy of different species Myrtasya as the antibacterial and antifungal compounds used in health products, and food industry. Using natural antimicrobials are interesting strategies for reducing the use of chemical additives in the food industry. Essential oils (EOs) are defined as a mixture of volatile water insoluble substances to be incorporated into the edible films due to exhibit antimicrobial effects. Moreover, evaluation EOs on the physical, optical and structural properties of the resulting film is also important. Therefore, the aims of this work were to determine the effect Eucalyptus globulus essential oil on antibacterial properties (2) to determine the antimicrobial activity of chitosan based films containing Eucalyptus globulus essential oil against S. aureus, B. cereus, E. coli and S. intertidis.

Materials and methods: The foodborne microbial strains were prepared from Persian Type Culture Collection. The essential oil was analyzed by gas chromatography (GC) (Thermoquest 2000, UK). In this study, the antimicrobial activity of Eucalyptus globulus essential oils (EGOs) was evaluated against two gram positive (S. aureus and B. cereus) and two gram negative (E. coli and S. intertidis) bacteria by the agar diffusion technique and microdilution test. The effect of EGO was evaluated in liquid media and vaporous phase too. Chitosan solution were prepared by dissolving 1.5 % (W/V) of chitosan in aqueous solution containing 0.7% (V/V) of acetic acid under a magnetic stirrer at 40°C until chitosan was completely dissolved. Glycerol as plasticizer (10% weight of chitosan powder) was added to the solution and stirred for 10 minutes. The EGO with concentrations of 0.5, 1 and 1.5% v/v was added to the solution and was stirred for 5 minutes. The film forming solutions using a homogenizer (Heidolph, Germany) were homogenized with 12000 rpm for 4 min, then degassed for 5 min and 25 ml were cast on a 10 cm diameter petri dish. After drying the film in the oven at 38°C for 24 h, they were peeled from the plate surface and were evaluated. The antimicrobial activity of the films was evaluated in contact with liquid and vaporous media.

Results and discussion: Minimum inhibition concentration for gram negative (E. coli, S. enteritidis) and gram positive (B. cerus and S. aureus) bacteria showed 3.125 and 1.562 µg/l respectively. The inhibition zone for gram positive bacteria was bigger than gram negatives. The effect of EGO on bacteria based on Log reduction value (LRV) of S. aerus > B. cerus > E. coli> S. enteritidis. Thses results confirmed that gram positive bacteria were more sensitive to inhibition by plant essential oils than the gram-negative bacteria. Our results showed that chitosan film containing 1 and 1.5 % essential oil was able to reduce the density of bacteria. The Log reduction value of chitosan bioactive film was increased by increasing the concentration of E. globulus essential oil than 0.5 to 1.5 % in liquid media. The results of this work had demonstrated that chitosan bioactive film containing 1.5% EGO can be used an effective antimicrobial film for food packaging in direct contact.

Conclusion: Chitosan is a good biopolymer for active food packaging. The result of this study showed that chitosan films containing EGO could be used as active films due to enhanced the antimicrobial properties which are important in food packaging applications. Films containing essential oil had unique properties that are useful for coating of perishable foods such as fish and poultry.

Keywords

کشیری، م.، مقصودلو، ی.، خمیری، م. و بهروز، ر.، 1393، ارزیابی خواص ضدباکتریایی فیلم زیست فعال زئین حاوی اسانس آویشن شیرازی، فصلنامه علوم و صنایع غذایی، شماره 50، ص 195-206.
Adams, R. P., 2001, Identification of essential oils components by gas chromatography/quadrupole. Mass spectroscopy. Allured, Carol Stream, Illnois. New York: Spinger
Bakkali, F., Averbeck, S., Averbeck, D. & Idaomar, M., 2008, Biological effects of essential oils – A review. Food and Chemical Toxicology, 46, 446–475.
Botsoglou, N. A., Christaki, E., Fletouris, D. J., Florou-Paneri, P. & Spais, A. B., 2002, The effect of dietary oregano essential oil on lipid oxidation in raw and cooked chicken during refrigerated storage. Meat Science, 62(2), 259-265.
Brooker, M. & Kleinig, D. A., 2004, Field Guide to Eucalyptus. 2nd Edn., Vol. 8. Melbourne : Bloomings Books.
Cimanga, K., Kambu, K., Tona, L., Apers, S., De Bruyne, T., Hermans, N., & Vlietinck, A. J., 2002, Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of ethnopharmacology, 79(2), 213-220.
Coppen, J. J. W., 2002, Eucalyptus: The Genus Eucalyptus (Mediacinal and Aromatic Plants–Industrial Profiles). London and NewYork: Taylor and Francis, 440.
Draughon, F. A., 2004, Use of botanicals as biopreservatives in foods. Food and Agriculture Organization of the United Nations, 58(2), 20-28.
Duke, J. A. & Beckstrom-Sternberg, S., 1994, Potential for synergistic action of phytochemicals in spices. In: Amsterdam: Elsevier Science.
Ghalem, B. R. & Mohamed, B., 2008, Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. African Journal of Pharmacy and Pharmacology, 2, 211–215.
Ghanem, A. & Skonberg, D., 2002, Effect of preparation method on the capture and release of biologically active molecules in chitosan gel beads. Journal of applied polymer science, 84(2), 405-413.
Gilles, M., Zhao, J., An, M. & Agboola, S., 2010, Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chemistry, 119, 731–737.
Guarda, A., Rubilar, J. F., Miltz, J. & Galotto, M. J., 2011, The antimicrobial activity of microencapsulated thymol and carvacrol. International journal of food microbiology, 146(2), 144-150.
Inouye, S., Takizawa, T. & Yamaguchi, H., 2001, Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. Journal of Antimicrobial Chemotherapy, 47, 565–573.
Jones, R. N., 2001, Resistance patterns among nosocomial pathogens: trends over the past few years. Chest Journal, 119(2_suppl), 397S-404S.
Kim, S., 2008, Processing and properties of gluten/zein composite. Bioresource technology, 99(6), 2032-2036.
Kurita, K., 2006, Chitin and chitosan: functional biopolymers from marine crustaceans. Marine Biotechnology, 8(3), 203-226.
Lis‐Balchin, M., Hart, S. L. & Deans, S. G., 2000, Pharmacological and antimicrobial studies on different tea‐tree oils (Melaleuca alternifolia, Leptospermum scoparium or Manuka and Kunzea ericoides or Kanuka), originating in Australia and New Zealand. Phytotherapy research, 14(8), 623-629.
Muriel-Galet, V., Cerisuelo, J. P., LopezCarballo, G., Lara, M., Gavara, R. & Hernandez-Munoz, P., 2012, Development of antimicrobial films for microbiological control of packaged salad. International Journal of Food Microbiology. 157: 195–201.
Muriel-Galet, V., Cerisuelo, J. P., Lopez-Carballo, G., Lara, M., Gavara, R. & Hernandez-Muñoz, P., 2012, Development of antimicrobial films for microbiological control of packaged salad. International journal of food microbiology, 157(2), 195-201.
Ojagh, S. M, Rezaei, M., Razavi, S. H. & Hosseini, S. M. H., 2010, Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chemistry, 122,161-166.
Ouattara, B., Simard, R. E., Holley, R. A., Piette, G. J. P. & Begin, A., 1997, Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. International journal of food microbiology, 37(2), 155-162.
Oussalah, M., Caillet, S. & Lacroix, M., 2006, Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157:H7 and Listeria monocytogenes. Journal of food protection, 69,1046–1055.
Pitarokili, D., Tzakou, O., Loukis, A. & Harvala, C., (2003). Volatile metabolites from Salvia fruticosa as antifungal agents in soilborne pathogens. Journal of Agricultural and Food Chemistry, 51, 3294–3301.
Pyla, R., Kim, T. J., Silva, J. L. & Jung, Y. S., 2010, Enhanced antimicrobial activity of starch-based film impregnated with thermally processed tannic acid, a strong antioxidant. International journal of food microbiology, 137(2), 154-160.
Quintavalla, S. & Vicini, L., 2002, Antimicrobial food packaging in meat industry. Meat science, 62(3), 373-380.
Saidana, D., Mahjoub, M. A., Boussaada, O., Chriaa, J., Cheraif, I., Daami, M & Helal, A. N., 2008, Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae). Microbiological research, 163(4), 445-455.
Salarbashi, D., Tajik, S., Shojaee-Aliabadi, S., Ghasemlou, M., Moayyed, H., Khaksar, R. & Noghabi, M. S., 2014, Development of new active packaging film made from a soluble soybean polysaccharide incorporated Zataria multiflora Boiss and Mentha pulegium essential oils. Food chemistry, 146, 614-622.
Sartorelli, P., Marquioreto, A. D., Amaral-Baroli, A., Lima, M. E. & Moreno, P. R., 2007, Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus. Phytotherapy Research, 21, 231–233.
Shan, B., Cai, Y., Brooks, J. D. & Corke, H., 2007, Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomum burmannii): activity against foodborne pathogenic bacteria. Journal of Agricultural and Food Chemistry. 55 (14): 5484–5490.
Shen, L., Worrell, E. & Patel, M., 2010, Present and future development in plastics from biomass. Biofuels, Bioproducts and Biorefining, 4(1), 25-40.
Shieh, J. C., 1996, Yields and chemical components of essential oils in Eucalyptus camaldulensis leaves. Taiwan Journal of Forest and Science, 11, 149–157.
Silva, J., Abebe, W., Sousa, S. M., Duarte, V. G., Machado, M. I. L. & Matos, F. J. A., 2003, Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. Journal of ethnopharmacology, 89(2), 277-283.
Soliman, K. M. & Badeaa, R. I., 2002, Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food and chemical toxicology, 40(11), 1669-1675.
Sonboli, A., Babakhani, B. & Mehrabian, A. R., 2006, Antimicrobial activity of six constituents of essential oil from Salvia. Zeitschrift für Naturforschung C, 61, 160–164.
Su, Y. C., Ho, C. L., Wang, E. I. & Chang, S. T., 2006, Antifungal activities and chemical compositions of essential oils from leaves of four eucalyptus. Taiwan Journal of Forest and Science, 21, 49–61.
Suresh, P., Ingle, V. K. & Vijayalakshmi, V., 1992, Antibacterial activity of eugenol in comparison with other antibiotics. Journal of food science and technology, 29(4), 254-256.
Synowiecki, J. & Al-Khateeb, N. A., 2003, Production, properties, and some new applications of chitin and its derivatives. National Center of Biotechnology Information, 43(2):71-145
Takahashi, T., Kokubo, R. & Sakaino, M., 2004, Antimicrobial activities of Eucalyptus leaf extracts and flavonoids from Eucalyptus maculate. Letters in Applied Microbiology, 39, 60–64.
Tsiri, D., Kretsi, O., Chinou, I. B. & Spyropoulos, C. G., 2003, Composition of fruit volatiles and annual changes in the volatiles of leaves of Eucalyptus camaldulensis Dehn growing in Greece. Flavour and Fragrance Journal, 18, 244–247.
Tyagi, A. & Malik, A., 2010, In situ SEM, TEM and AFM studies of the antimicrobial activity of lemon grass oil in liquid and vapour phase against Candida albicans. Micron, 41, 797–805.
Tyagi, A. K. & Malik, A., 2011, Antimicrobial potential and chemical composition of Eucalyptusglobulus oil in liquid and vapour phase against food spoilage microorganisms. Food Chemistry, 126(1), 228-235.
Vilela, G. R., de Almeida, G. S., D'Arce, M. A. B. R., Moraes, M. H. D., Brito, J. O., da Silva, M. F. D. G., & da Gloria, E. M., 2009, Activity of essential oil and its major compound, 1, 8-cineole, from Eucalyptus globulus Labill., against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare. Journal of Stored Products Research, 45(2), 108-111.
Yanishlieva, N. V., Marinova, E. M., Gordon, M. H. & Raneva, V. G., 1999, Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chemistry, 64(1), 59-66.
Zheng, L. Y. & Zhu, J. F., 2003, Study on antimicrobial activity of chitosan with different molecular weights. Carbohydrate Polymers, 54(4), 527-530.
Zivanovic, S., Chi, S. & Draughon, A. F., 2005, Antimicrobial activity of chitosan films enriched with essential oils. Journal of food science, 70(1), M45-M51
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