با همکاری انجمن علوم و صنایع غذایی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه تربیت مدرس

2 دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

به منظور بهینه‌سازی شرایط استخراج ژلاتین از پوست کوسه چانه سفید (Carcharhinus dussumieri)، تأثیر غلظت سود (1 -01/0 نرمال)، غلظت اسیدکلریدریک (1 – 01/0 نرمال) و زمان استخراج (8- 3 ساعت)، بر بازده استخراج و نقطه ذوب ژلاتین با استفاده از طرح مرکب مرکزی به روش سطح پاسخ (RSM) مورد بررسی قرار گرفت. بر اساس مدل‌های رگرسیونی به‌دست آمده، مقادیر پیش‌بینی شده در شرایط بهینه شامل 222/23 درصد (بازده استخراج) و°C 224/32 (نقطه ذوب) بوده اند. شرایط اپتیمم با استفاده از سود 01/0 نرمال، اسید کلریدریک 135/0 نرمال و مدت زمان استخراج 3 ساعت به‌دست آمد. نتایج نشان داد که غلظت اسید کلریدریک و غلظت سود به شکل معنی‌داری بر بازده استخراج و نقطه ذوب ژلاتین حاصل، اثر داشت (p≤0/05)، ولی مدت زمان استخراج تأثیر معنی‌داری بر هیچ یک از دو ویژگی مورد بررسی نداشت.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Processing Conditions on the Extraction Yield and Melting Point of Whitecheek Shark (Carcharhinus dussumieri) Skin Gelatin

نویسندگان [English]

  • Masoud Rezaei 1
  • Ali Motamedzadegan 2

1 Tarbiat Modares University

2 Sari University of Agricultural Sciences and Natural Resources

چکیده [English]

To investigate the optimum conditions for extracting gelatin from whitecheek shark (Carcharhinus dussumieri (skin, response surface methodology (RSM) has been adopted. In this research, the effect of NaOH concentration (0.01-1 N), HCl concentration (0.01-1 N), and extraction time (3-8 h) on dependent variables, yield and melting point of the skin gelatin have been studied using central composite design. Based on the individual regression models, predict values of multiple response at optimal condition were %23.222 (yield), and 32.224 °C (melting point). The best pretreatment conditions to get to the optimum properties were 0.01 N NaOH, 0.135 N HCl and 3 h extraction time. The results showed that, NaOH and HCl concentrations had significant effects, but extraction time had no significant effect on gelatin extraction yield and its melting point.

کلیدواژه‌ها [English]

  • Whitecheek shark
  • Gelatin
  • RSM
  • yield
  • Melting point
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