Research Article
Food Biotechnology
Soheyl Reyhani Poul; Sakineh Yeganeh; Reza Safari
Abstract
IntroductionOne of the synthetic and harmful preservatives used in sausage formulation is sodium nitrite. This compound helps to increase the shelf life and marketability of meat products by preventing the growth of anaerobic bacteria, especially clostridium, exerting an antioxidant effect, stabilizing ...
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IntroductionOne of the synthetic and harmful preservatives used in sausage formulation is sodium nitrite. This compound helps to increase the shelf life and marketability of meat products by preventing the growth of anaerobic bacteria, especially clostridium, exerting an antioxidant effect, stabilizing the red color of meat and improving the taste. Despite these benefits, sodium nitrite is very dangerous for health and it can cause malignant diseases. For this reason, it is necessary to replace this substance using a natural preservative. Pigments extracted from aquatics such as astaxanthin due to having antioxidant activity, antimicrobial properties and pink color may be a good substitute for sodium nitrite. However, these pigments must be nanoencapsulated at first due to their sensitivity to food processing conditions, including high temperatures. The aim of the current research at the first was to extract astaxanthin from Haematococcus pluvialis microalgae using the acid-acetone method and pigment nanoencapsulation using maltodextrin-sodium caseinate combined coating. Then, sodium nitrite in the sausage formulation was replaced by the carrier nanocapsules with different proportions and oxidative and microbial spoilage tests, color and sensory evaluations were performed for different treatments. Materials and MethodsAt first, astaxanthin pigment was extracted from Haematococcus pluvialis using the acid-acetone technique. Then, the extracted pigment was nanoencapsulated using maltodextrin-sodium caseinate combined coating and the resulting (carrier) nanocapsules in the form of treatments A (120 mg/kg sodium nitrite), B (120 mg/kg nanocapsules carrying astaxanthin), C (90 mg/kg sodium nitrite+30 mg/kg nanocapsules carrying astaxanthin), D (60 mg/kg sodium nitrite+60 mg/kg nanocapsules carrying astaxanthin) and E (30 mg/kg sodium nitrite+90 mg/kg nanocapsules carrying astaxanthin) were replaced sodium nitrite in the sausage formulation. These treatments were evaluated in terms of oxidative and microbial spoilage, color indices and sensory properties during 28 days of storage at refrigerator along with the control (without sodium nitrite and carrier nanocapsules). This research was conducted in a completely randomized design. Data were analyzed by one-way analysis of variance and the difference between the means was evaluated by Duncan's test at 95% confidence level. Results and DiscussionAccording to the results, the lowest levels of thiobarbituric acid and peroxide value during the storage period were related to treatments B, E and D (p>0.05). Treatments A and C had no significant difference in terms of thiobarbituric acid and peroxide value until day 14 (p>0.05), but with increasing storage time, this difference became significant and treatment A showed higher values (p<0.05). The results of this section showed that the power of astaxanthin in controlling oxidative spoilage is significantly greater than sodium nitrite, and if the purpose is only to control this type of spoilage, there is no need to replace or use sodium nitrite. The results also showed that in terms of controlling microbial spoilage, sodium nitrite has more power than nanocapsules carrying astaxanthin. So that, the lowest amount of total volatile basic nitrogen (TVB-N) and the most standardized pH were related to treatments A, C and D (p>0.05) during the storage period (p<0.05). Treatments B and E (p>0.05) were ranked next (p<0.05) in terms of the two mentioned indicators. The results of this section showed that if sodium nitrite reduced from 120 mg/kg to 60 mg/kg and replaced by nanocapsules carrying astaxanthin in the sausage formulation, the resulting product has the same antimicrobial power as the product containing 120 mg/kg sodium nitrite. Evaluation of the color and sensory properties of treatments showed that A, C and D treatments are at a higher level than B, E (treatments) and control in terms of color indices and general acceptance (p<0.05). The comparison of the color indices and sensory properties of the treatments on days 0 and 28 of storage at refrigerator showed that the color and sensory indices remained constant in the formulated treatments, unlike the control. Conclusion Nanocapsules carrying astaxanthin with maltodextrin-sodium caseinate combined coating as a natural product with many properties in health, control and prevention of various diseases, have a high efficiency to replace the sodium nitrite in sausage formulation. So that, if 30 to 60 mg/kg of the permissible limit of 120 mg/kg of sodium nitrite in the sausage formulation is replaced by nanocapsules carrying astaxanthin, the resulting product will be similar to the product containing 120 mg/kg of sodium nitrite in terms of shelf life, resistance to oxidative and microbial spoilage, color indices and sensory properties.
Research Article
Food Chemistry
Atena Modiri Dovom; Akram Arianfar; Sara Naji-Tabasi; Vahid Hakimzadeh
Abstract
IntroductionMayonnaise is a products which is widely popular in most countries. Apart from the desirable taste of this product as a seasoning, plays an effective role in providing nutrients and energy for humans. Dietary mayonnaise, is semi-solid or liquid product prepared from emulsification fat substitutes ...
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IntroductionMayonnaise is a products which is widely popular in most countries. Apart from the desirable taste of this product as a seasoning, plays an effective role in providing nutrients and energy for humans. Dietary mayonnaise, is semi-solid or liquid product prepared from emulsification fat substitutes and vegetable oils with vinegar and other additives with less energy and fat. Fat has more calories (9 kcal/g) compared to protein and carbohydrates (4 kcal/g). Mayonnaise is an oil-in-water emulsion and due to having high amounts of fat it causes cardiovascular diseases. Gums are part of construction formula in low fat products to create texture. Due to the great desire to consume low-fat products and also the wide use of this sauce, production of low-fat mayonnaise is important. The purpose of this research was to develop reduced fat mayonnaise using stabilized nano emulsion with casein complex Pickering and Gadomeh Shirazi gum. Materials and MethodsMayonnaise with reduced oilcontains 30, 40 and 50 percent Pickering emulsion replacement oil respectively at the level of 42, 32 and 22 percent produced and compared with the control sample. Centrifugal and time stability tests, textural features, color characteristics, morphology, organoleptic properties were evaluated. Results and DiscussionThe results showed as the replacement percentage increases nano emulsion containing Pickering particles and reducing the percentage of fat in mayonnaise emulsion stability she found her mayonnaise sauce although at a replacement level of 30% nano emulsion, this decrease in stability was not significant (P<0.05). In the time stability test low-fat mayonnaise with an increase in the percentage of nano emulsion replacement, After 90 day’s significant difference between the control sample and mayonnaise no significant difference was observed with oil reduced by 30 and 40%. In the colorimetry test it showed that among the factors L*. a*. b statistically with the sample there was a significant difference (P≤0/05). Brightness In the witness sample, it was 48/85 in connection with RFM50% the least complex of particles Gadomeh Shirazi gum and casein protein and in relation to RFM30% the most complex of particles Gadomeh Shirazi gum and casein were used. Due to the presence of nanoemulsion contains complex particles Gadomeh Shirazi gum particles and protein. The brightness has decreased in general, from the RFM50% sample up to RFM 30% samples simultaneous with increasing amount of nanoemulsion and reduce the amount of fat the brightness is reduced. ConclusionBy replacing the nano emulsion and reducing the amount of fat in the structure of the sauce the amount of tissue stiffness decreased and pheneritis increased. The sensory test of the samples showed, the witness sample has the most general acceptance but there is a significant difference between the samples there was no reduced-fat mayonnaise with the control. Investigating the characteristics of mayonnaise with reduced fat using nanoemulsion stabilized with complex Pickerings casein and Shirazi Gadomeh gum the results showed that with the increase, reduce the amount of oil up to 30%, Sensory and texture characteristics in a meaningful way decreased. But oil reduction up to 50% in the presence of Pickering nanoemulsion preserve textural features and promoted in some cases. All emulsions produced of favorable stability during storage and centrifugation had. Best stability in control and sample RFM-30% was observed. Based on the results, use of nanoemulsion maintains quality characteristics mayonnaise with oil especially reduced in the sample RFM 30%, it was. Also note to the point that Pickering emulsion structure in the digestive system high stability against digestion, is hope Pickering nanoemulsion structure in the production of food products various low-fat items be investigated further.
Research Article
Food Technology
Mahshad Lavarsetagh; Nafiseh Zamindar; Yasaman Esmaeili
Abstract
Introduction The first quality attribute about food evaluated by consumer is visual characteristics. The apparent attributes of a product include its shape, color, packaging, size, and uniformity. The consumer considers the product's appearance in the first step before any other information about ...
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Introduction The first quality attribute about food evaluated by consumer is visual characteristics. The apparent attributes of a product include its shape, color, packaging, size, and uniformity. The consumer considers the product's appearance in the first step before any other information about other characteristics, such as taste or smell, especially in the first purchase. Color is the most crucial indicator of the quality of food that affects consumer acceptance. To replace the color lost during the process, increasing the existing color and minimizing variation during processing; natural colors, synthetic and inorganic colorants are added to food. Red beet is one of the most important natural sources of color; the essential pigment in this plant is betalain, composed of two subunits, betacyanin, and betaxantine. Considering the growing demand to develop more effective methods for extracting active compounds present in vegetable materials, including naturally occurring betalain and other nutritious compounds, this research was conducted to optimize the extraction conditions of this pigment from red beet. Materials and Methods Preparation of red beet The red beets were washed, peeled, and grated to prepare the sample. Then, red beets were dried in an oven at 30 °C for 48 hours and milled. For extraction, 10 grams of red beet powder was mixed with 200 ml of solvent (water-ethanol in a ratio of 1:1) and subjected to ultrasonication at 37 kHz. Experiments in 27 runs were performed based on a historical data design, randomized subtype and quadratic model. After the implementation with the edition of Design Expert 7 software, the range of variables pH (3-5), temperature (15-25 °C), and time (10-20 min) were applied for extraction. The pulp was dried using a Wattman filter paper in an oven at 35 °C and used for subsequent tests. Yield measurements The efficiency of the extraction process was calculated by dividing the weight of the extract obtained by the weight of the beetroot. Measurement of pigments The betacyanin and betaxanthine pigments show maximum absorption in 522-559 nm and 474-480 nm, respectively. To measure betacyanin and betaxanthine in extracts, a certain amount of powder was dissolved in distilled water, and its absorbance was measured at 530 and 480 nm. The amount of pigments obtained from different extraction conditions was compared. Results and Discussion In this study, the overall results showed that an increase in the extraction pH and time increased the extraction of betacyanine and betaxanthine. It would be better to extract betacyanine at temperatures below 25 °C since this pigment is sensitive to high temperatures. The maximum yield was observed when pH value, temperature, and time were 5, 21 °C, and 20 min, respectively. Comparison of experimental and predicted values of the proposed responses at the proposed optimal points by t-test showed no significant difference (p<0.05). A quadratic model was suggested for the responses, and the lack of fit was not significant (p<0.05). The extraction of betaline from red beet using the ultrasonic assisted method causes a reduction in time, temperature, energy, and solvent consumption. ConclusionThe maximum yield was observed when pH value, temperature, and time were 5, 21 °C, and 20 min, respectively. Comparison of experimental and predicted values of the optimal points showed no significant difference by t-test (p<0.05). According to the results obtained from this research, ultrasonic assisted extraction can be considered an efficient and fast method. Extraction of betalain from red beetroot with ultrasonic assisted method is more efficient. It can replace the regular practice of extraction in the industry due to the reduction of time, temperature, energy, and solvent consumption, thus reducing cost and increasing profit.
Research Article
Food Technology
Zahra Dehghan; Elnaz Milani; Neda Hashemi
Abstract
IntroductionGastrointestinal diseases are very important among human societies, especially in developing countries. Celiac disease is one of these diseases, caused by the interference of gluten in food, the body's immune system, genetics and environmental factors. Therefore, it is necessary to ...
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IntroductionGastrointestinal diseases are very important among human societies, especially in developing countries. Celiac disease is one of these diseases, caused by the interference of gluten in food, the body's immune system, genetics and environmental factors. Therefore, it is necessary to provide a solution for the production of gluten-free products and also to improve their quality. The tremendous growth in the nutritional knowledge of the public has caused the development and production of healthy food products for certain groups. Baked products such as cookies are very popular among the society because of their textural characteristics as well as flavoring and attractive colors. Therefore, their enrichment is of interest. Most commercial gluten-free bakery products are based on pure starch or the combination of corn starch with gluten-free flour, which is associated with dryness and sandiness in the product. Materials and MethodsThe aim of this research was to investigate the functional characteristics of non-extruded and extruded chickpea flour samples and then the effect of adding different levels of it (0, 20 and 40%) on physicochemical, Lightness, porosity and sensory characteristics using completely randomized factorial design. The blend of chick pea flour- xanthan gum was extruded by a parallel twin-screw extruder (Jinan Saxin, China). Process was performed with die of 3 mm, and extrusion temperature of 140 ℃. The chemical composition of raw materials was measured by standard AOAC (2000) methods. The hardness of cookies was measured using Texture Analyzer (TA plus Ametek, UK). The cylinder steel probe (2 mm diameter) was set to move at a speed of 1 mm/s. Samples were punctured by the probe to a distance of 10 mm. The color values of L* (lightness), a*(redness), b*(yellowness) of the samples were determined by the Hunterlab instrument (Reston VA, US) (Rhee et al., 2004). Water absorption index in terms of grams of bonded water was calculated per gram of dry material in percent. Sensory evaluation was performed using a 5-point hedonic test. Results and DiscussionIn general, gluten-free products are unable to maintain carbon dioxide gas due to the lack of a coherent and uniform gluten network, which causes an increase in volume. As a result, the product is small in volume and the structure of the crumb is compressed. The extrusion baking process had a high potential to improve cookie quality. In such a way that the addition of extruded chickpea flour increased the porosity of the cookie samples. The results of the texture analysis show that the addition of extruded chickpeas up to a 40% improved the texture of the cookie and also increased its shelf life. Also, the sensory test results showed that the favorable effect of adding extruded chickpea flour up to 20%. Consequently, by summarizing the results of physical and sensory tests, it was determined that cookies with appropriate sensory and quality characteristics can be produced using 40% of extruded chickpea flour. Peas, and especially extruded peas, rich in protein and dietary fiber content and high water absorption ability, led to increase the moisture and reduced the hardness of the cookie texture. Considering the increase in demand for gluten-free products, it seems that enriching these products with nutrients such as chickpea flour can be an alternative method to improve the nutritional value of these products. Adding legumes is a good way to increase the consumption of legumes, which are rich in the amino acid lysine. Legumes, especially peas, have high nutritional value and functional characteristics, and including them in the diet by adding them to bakery products is a good way to increase their consumption. The use of chickpea flour as a nutrient source in cookie formulation increases the nutritional value, reduces the glycemic index and improves the variety of such products.
Short Article
Food Chemistry
Zahra Khodakaramifard; Hannan Lashkari
Abstract
Introduction
The date palm (Phoenix dactylifera L.) plays an important social, environmental, and economical role for many people living in arid and semiarid regions of the world. Date fruit is one of the major agricultural crops in the East Asia region, where about 90% of the world's dates are cultivated. ...
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Introduction
The date palm (Phoenix dactylifera L.) plays an important social, environmental, and economical role for many people living in arid and semiarid regions of the world. Date fruit is one of the major agricultural crops in the East Asia region, where about 90% of the world's dates are cultivated. Dates are rich in certain nutrients and provide a good source of rapid energy, due to their high carbohydrate content (70–80%). Moreover, date fruits contain fat (0.20–0.50%), protein (2.30–5.60%), dietary fibre (6.40–11.50%), minerals (0.10–916 mg/100 g dry weight), and vitamins (C, B1, B2, B3, and A) with very little or no starch. In addition to the direct consumption of the fruit, various industrial products are also extracted derived from this product, including date juice, date honey, liquid sugar, vinegar, alcohol, caramel, date paste and date chocolate. The annual production of one million and 400 thousand tons of dates in Iran has made Iran the second pole of date production in the world after Egypt. Zarin Dasht region is located in Fars province, and the annual production of dates in this region reaches more than 1000 tons. The aim of the present work was to investigate the chemical composition, carbohydrate, and antioxidant capacity of two cultivars of Zarin Dasht dates.
Materials and Methods
After collection, all date fruits were washed with tap water, and the seeds were then removed, and the flesh were shade dried at room temperature. The dimensions and area of the imaged surfaces were measured by the physical properties measurement device in 100 repetitions. The working principle of this device is based on image processing technique. By placing the product in three different positions and perpendicular to each other, pictures of the date samples were taken individually. Date mass was obtained using a sensitive digital scale with an accuracy of 0.01 g. The displaced water method was used to determine the volume and density of each date seed. Bulk density, date porosity, geometric mean diameter, sphericity coefficient and surface area of the samples were determined. The amount of moisture was determined by weight method, ash by burning in an electric furnace, titratable acidity based on malic acid and pH of the samples were measured by a digital pH meter. To measure the amount of total phenol in the fruit, Folin–Ciocalteu reagent was used and the absorbance of the reaction mixture was read at 750 nm by a spectrophotometer. The amount of total phenol was reported in terms of gallic acid. The antioxidant capacity was determined through the neutralization of free radical 2 and 2 diphenyl 1-picrylhydrazyl (DPPH). To measure the sugar of all samples, first a standard curve was drawn from the glucose solution in different concentrations, then the sugar content of the samples was measured in milligrams per gram of fresh weight at 490 nm using the sulfuric phenol method. The amount of crude fibre was calculated according to the standard method of AOAC-991/43. The amount of fat was obtained with the Universal Extractor E-800 device for 3 hours at a suitable temperature and in 250 cc of n-hexane solvent. Finally, the statistical analysis of the data was done factorially and in the form of a completely random design in 3 replications using SAS 4, 9 software and the comparison of the means was done using the LSD test at a probability level of 1%.
Results and Discussion
According to the results of this research, there was a significant difference in all qualitative traits except pH (P<0.01). In comparing the characteristics of the palms of two cultivars, it was observed that the highest amount of fibre (1.78 %), titratable acid (0.59 %), ash (1.64 %) and fat (0.85 %) is related to Shahani cultivar,and the highest amount of total phenol (8.1 mg/gFW), DPPH inhibitory property (13 %), moisture (18.7%), sugar (63.8 %), protein (0.29 %) and pH (5.74) belonged to Khassui cultivar. Also, comparing the kernel characteristics of two cultivars, it was observed that the highest amount of ash (3.17 %), total phenol (10.8 mg/gFW), antioxidant property (72 % DPPH inhibition), protein (2.55 %), pH (6.11) and fat (9.20 %) related to the kernel of Shahani variety and the highest amount of fibre (26.2 %), moisture (5.26 %), sugar (15.8 %) and titratable acid (0.38 %) belonged to the kernel of Khassui cultivar. Overall, the kernel of Shahani variety had more DPPH inhibitory power among all the samples.
Research Article
Food Technology
Ebrahim Taghizadeh; Mohammad Alizadeh khaled abad; Hamed Hassanzadeh
Abstract
IntroductionEdible films and coatings are suitable for fresh, semi-processed foods, and also for processed fruits and vegetables because they can increase their shelf life, protect them from the activity of microorganisms, and ultimately improve their nutritional and sensory value. In addition, edible ...
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IntroductionEdible films and coatings are suitable for fresh, semi-processed foods, and also for processed fruits and vegetables because they can increase their shelf life, protect them from the activity of microorganisms, and ultimately improve their nutritional and sensory value. In addition, edible films and coatings are able to transfer compounds that may have been added to improve the performance of the coating film and better preserve the product or have benefits for the consumer. The present research was conducted with the aim of investigating the structural physicochemical properties of whey protein concentrate smart film containing pomegranate and red grape anthocyanins and zinc oxide nanoparticles. Material and MethodTo prepare nano composite film, 10 grams of whey protein concentrate powder was added to 90 ml of distilled water. At the beginning of the production of the film, the pH was adjusted to pH = 8 with the help of 0.1 normal soda, and in order to better dissolve the isolate, the solution was heated for 30 minutes at 80 degrees Celsius to ensure denaturation. It was then placed on a magnetic stirrer. After cooling the solution, pomegranate and red grape anthocyanins were added to the solution separately according to the statistical plan. The solution was then homogenized for 10 minutes at 13,000 rpm, and finally zinc oxide nanoparticles were added to the solution according to the statistical plan and homogenized on a magnetic stirrer for 10 minutes, and then in an ultrasound bath to disperse for 10 minutes In the next step, glycerol was added to the 40% of the weight of the dry substance and placed on a rotating magnetic stirrer without heat for 15 minutes. The formed solution was aerated for 10 minutes and 25 ml of it was poured in the center of the plate (8 cm). Then the film was dried in the oven at 38 degrees Celsius and the control film was produced. After preparing the films, the color of the film samples was determined by measuring the color components L* (lightness), a* (red/green) and b* (yellow/blue) using a Hanterlab colorimeter. The thickness of the films was measured by a digital micrometer with an accuracy of 0.001 mm at 10 random points of each film. Finally, humidity, solubility and FTIR measurement tests were also performed for the prepared films. The preparation of whey protein concentrate smart composite film was investigated based on two variables: red grape anthocyanin/pomegranate anthocyanin percentage and copper oxide nanoparticle percentage. Each of which was investigated in five levels and the response surface methodology (RSM) of the central composite design was used. A total of 24 composite films were prepared and their physical and chemical properties were investigated. Also, after analyzing the data, the optimal movies were checked based on optimization and utility function by 11 Design expert statistical software. Results and DiscussionBy increasing the amount of anthocyanin and zinc oxide nanoparticles, the thickness of the layers increased up to the middle point (0.58-0.89 mm), and the largest increase in thickness was observed in samples with 1.5 cc of anthocyanin and 2% of zinc oxide nanoparticles. Also, films with low concentrations of anthocyanin (0.1-4 cc) and a high percentage of the selected range of zinc oxide (0.2-0.7%) show the highest percentage of solubility. The added zinc oxide nanoparticles increased the solubility of the films. FTIR analysis showed that the interactions between whey concentrate and anthocyanins were probably responsible for the changes in the properties of the composite layers. The utility function for each response was estimated by numerical methods and the general utility function was obtained for the optimal samples of red grape and pomegranate. For the optimal pomegranate, the obtained results include the amount of anthocyanin equal to 2.6 cc, zinc oxide nanoparticles 0.6 (weight/volume), solubility 65.37, thickness 0.64 mm, indices a, L and bwere. 27.88 0.24 and -2.01, respectively. Finally its total desirability has been reported as 0.58. The utility function for each answer was estimated by numerical methods and the general utility function was obtained for the optimal samples of red grapes such as the optimal pomegranate. For the optimal grape film samples, the obtained results include: the amount of anthocyanin equal to 1.38 cc, 3.4 zinc oxide nanoparticles (weight/volume), solubility 59.83%, thickness 0.83 mm, a, L and b indices were 27.03, 7.05 and 1.92 respectively and, its total desirability was 0.63.
Research Article
Food Biotechnology
Mina Rasoulifar; Alireza Sadeghi; Fahimeh Hajinia; Maryam Ebrahimi; Mohammad Ghorbani
Abstract
IntroductionWheat bread is a staple food worldwide, and bread making using sourdough is one of the oldest technologies. In this regard, the use of legume sourdough is important in improving the quality of wheat bread. Sprouted legumes are also proper substrates rich in bioactive compounds for sourdough ...
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IntroductionWheat bread is a staple food worldwide, and bread making using sourdough is one of the oldest technologies. In this regard, the use of legume sourdough is important in improving the quality of wheat bread. Sprouted legumes are also proper substrates rich in bioactive compounds for sourdough fermentation to produce enriched products. Furthermore, application of herbal extracts in sourdough formulation is a simple way to enhance organoleptic properties and mold-free shelf-life of the produced sourdough bread. Considering positive effects of sourdough on techno-functional properties of the produced bread, the aim of the present study was to enhance quality parameters of wheat bread using controlled fermented sprouted lentil containing fennel extract. Materials and MethodsIn the present study, predominant lactic acid bacteria (LAB) were isolated using the sequential back-slopping process from fermented sprouted lentil containing fennel extract. The isolates were screened based on their antifungal activities against Aspergillus niger. The selected isolates were identified through amplification of the target sequence with 1500 bp from its 16S rDNA gene and sequencing of the PCR products. After that, controlled fermentation containing selected LAB isolates (as a starter culture) was performed in processing of wheat bread. Crumb hardness, specific volume, water activity, overall acceptability and surface growth rate of the target fungus were compared in the produced breads. In order to determine the effects of the substrate, fermentation and germination, suitable samples were produced. The results of the present study were also analyzed in a completely randomized design with three replications, and Duncan test at P<0.05 significant difference was used to compare the mean. Results and DiscussionSequencing results of the PCR products (amplicons) led to the identification of Pediococcus acidilactici as the selected LAB isolate. Addition of lentil sourdough to wheat bread significantly (P<0.05) reduced the weight loss and crumb hardness of wheat bread and also increased its specific volume without significant effect. Among the produced breads, the lowest specific volume was belonged to the sample containing lentil and the highest specific volume was observed in the control sample. Moreover, fermentation and sprouting reduced the surface growth of A. niger in the produced breads. The surface growth rate of the fungus in the bread containing fermented sprouted lentil was also significantly lower than those of the control sample. In addition, wheat bread containing fermented sprouted lentil received the highest overall acceptability score among the produced enriched breads. It is reported that the positive effects of controlled sourdough on textural features of the produced bread are mainly associated with the acidification activity of the starter culture used. Production of organic acids and other inhibitory metabolites by the sourdough starter culture is also involved in its antifungal activity in the product. Moreover, production of volatile and non-volatile aroma precursors during sourdough fermentation affect sensory attributes of the sourdough bread. ConclusionNowadays, consumer demand for healthy products with minimal processing and with no synthetic additives such as clean-label foods, and the use of bio-preservatives (application of microorganisms and their metabolites to prevent spoilage and increase the shelf-life of the product) has become a growing trend. Application of legumes as a good source of protein, other nutrients and essential components in bread formulation is important for their use in the daily diet. According to the results of the present study, controlled fermented sprouted lentil containing fennel extract can be used as an antifungal compound and texture improver in bakery industries. Overall, the use of plant extracts with antifungal effects as well as controlled fermentation of sourdough with selected LAB as protective/starter culture can reduce the use of chemical preservatives in bread.
Research Article
Food Biotechnology
Elnaz Mehrabi; Mojtaba Bonyadian; Aziz A. Fallah
Abstract
IntroductionRecently, the use of new packaging materials and natural additives for improving the durability and preservation of foods has been increasingly considered. Edible coatings containing plant extracts lead to increase the shelf life of food, do not cause environmental contamination, and do not ...
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IntroductionRecently, the use of new packaging materials and natural additives for improving the durability and preservation of foods has been increasingly considered. Edible coatings containing plant extracts lead to increase the shelf life of food, do not cause environmental contamination, and do not endanger the health of the consumer. Fish is a food rich in amino acids, vitamins and minerals, and polyunsaturated fatty acids, especially omega-3, which has made it more and more popular in the human diet. However, the presence of a significant amount of polyunsaturated fatty acids as well as easily digestible proteins has turned fish into a highly perishable commodity. Fresh seafood spoils rapidly due to the enzymatic and bacterial activities that occur after death, as well as the spoilage caused by the oxidation of polyunsaturated fatty acids and the high concentration of hematin compounds and metal ions of fish muscle, such as iron, and their pH which is close to neutral. Therefore, the use of natural coatings that can increase the shelf life of food and attract the consumers should be given more attention. Iran is the largest fishery producer in the region, thus, providing new methods to increase the shelf life of these products until the time of consumption can guarantee the safety and be effective in the economic prosperity of this industry. Therefore, the present study was designed to investigate the effect of chitosan coating along with the volatile oil of ginger plant on the shelf life of salmon fillets stored in refrigerator to reduce microbial growth, reduce chemical reactions, and increase the storage life of salmon fillets in refrigerator. Material and MethodsRainbow trout fish with an approximate weight of 600 grams were purchased from the market. Fish were washed with sterile distilled water, and cut into 60-gram pieces after separating the head and tail and eviscerating. The prepated fish were randomly divided into 4 groups. The control group was without any treatment but the treatment groups were immersed in chitosan containing different concentrations of Ginger essential oils. All the samples were kept in zipped bags at refrigerator (4°C) for 15 days. Microbial tests (Mesophilic, Psychrophilic, Coliform, and Lactic acid bacteria count), chemical tests (pH, TVN, TBARS), and sensory tests (color, smell, taste, texture, and overall acceptance) were performed on days 1, 4, 7, 12 and 15. The data obtained from the microbial and chemical tests were analyzed by one-way analysis of variance and the data obtained from the sensory tests were analyzed by the Kruskal-Wallis test in Sigma Stat 4 statistical software, considering P<0.05. Results and Discussion The results of bacterial tests showed that chitosan coating with ginger essential oil had a significant effect on reducing the growth of mesophilic, coliform, lactic acid bacteria and psychrophilic bacteria compared to the control and chitosangroups during 15 days of storage in the refrigerator (P<0.05). During the storage period, chitosan-treated groups containing 1.5% and 0.75% of ginger volatile oil had the best microbial quality in terms of mesophilic bacteria, Psychrophilic bacteria, lactic acid- bacteria, and coliforms. Also, in samples immersed in chitosan coating with ginger essential oil, the pH, TVN and TBARS values at the end of the storage period were significantly lower than the control and chitosan group (P<0.05). The results showed that pH, TVN, and TBARS did not exceed the defined standard for fish meat at the end of the storage period in the groups treated with ginger volatile oil. Sensory characteristicsindicated that the groups treated with chitosan coating containing ginger essential oils showed better sensory characteristics in terms of color, taste, smell, texture, and overall acceptance than the control and chitosan groups during the storage period (P<0.05). ConclusionBased on the results of the present study using the chitosan coating combined with ginger volatile oils has antimicrobial and antioxidant properties, which can reduce the oxidation of fats and microbial loads, while maintaining the organoleptic quality and increasing the shelf life of fish meat at refrigerator temperature. In comparison between treated groups, the use of chitosan along with 0.75% volatile oil of ginger is recommended, because by using a smaller amount of volatile oil, the microbial, chemical, and organoleptic properties can be kept at the standard level until the 15th day. Acknowledgments The authors are grateful to Shahrekord University's Research Vice-Chancellor for supporting this project.
Research Article
Food Technology
Aliehsadat Rafaathaghighi; Leila Jafari; Abdolmajid Mirzaalian Dastjerdi; Farzin Abdollahi
Abstract
Introduction
Ziziphus mauritiana, also known as Ber fruit, is a tropical fruit from the Rhamnaceae family with high nutritional value and a short shelf life. Edible coatings the surface of the fruit can play a positive role in increasing its shelf life. The application of alginate (Al), a linear polysaccharide ...
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Introduction
Ziziphus mauritiana, also known as Ber fruit, is a tropical fruit from the Rhamnaceae family with high nutritional value and a short shelf life. Edible coatings the surface of the fruit can play a positive role in increasing its shelf life. The application of alginate (Al), a linear polysaccharide derived from brown seaweed (Macrocystis pyrifera), which has the advantage of moisture retention, and also Shirazi thyme (Zataria multiflora) essential oil (ZEO), which contains antioxidant and antimicrobial compounds, can improve the fruit's characteristics and preserve its quality for a longer period. In recent years, the application of ZEO together with organic coatings in order to enhance the quality characteristics of different fruits during storage has been the focus of researchers. This study aims to investigate the effect of alginate coating and ZEO on the post-harvest and physicochemical characteristics of Ber fruit during storage at 6±1 C˚ and a relative humidity of 90-95%.
Material and Methods
This study was conducted to enhance the shelf life of Ber fruits by treating them with alginate (Al) and Shirazi thyme essential oil (ZEO). Ber fruits at their mature green stage were harvested in 2022 from a commercial orchard in Minab, Hormozgan province. After harvesting, the fruits were transported to the Horticulture Laboratory University of Hormozgan, to apply the treatments. Factors include six treatments (control, Shirazi thyme essential oil 0.5%, Shirazi thyme essential oil 1%, alginate, alginate + Shirazi thyme essential oil 0.5% and alginate + Shirazi thyme essential oil 1%) and five storage periods(zero, 7, 14, 21, 28 days). The treated fruits were then stored at 6±1 C˚, and relative humidity of 90-95% for 28 days. The factorial experiment was conducted as a completely randomized design with four replications, and ten fruits were used in each replication. Fruit parameters such as fruit weight loss, respiration rate, total soluble solids (TSS), organic acid, ascorbic acid, fruit firmness, carotenoids, and chlorophyll content were evaluated. Statistical analysis of data was done using SAS software. Figures were drawn using Excel software.
Results and Discussion
Based on the results of the analysis of variance, the main effects of time and treatment were significant (P<0.01) for all characteristics. Additionally, the interaction effect of treatment and time was significant (P<0.01) for all traits except for ascorbic acid. Comparisons of means showed that all treatments, maintained fruit quality compared to the control. The Al + ZEO treatment had a significant effect on reducing respiration rate. This combined treatment reduced weight loss, decay percentage, and fruit softening while slowing down the increase in TSS. The alginate coating and Shirazi thyme essential oil delayed ripening and subsequent fruit softening by reducing ethylene activity. Furthermore, the coated fruits had higher levels of ascorbic acid compared to the untreated fruits. These coatings are impermeable to water vapor and create a saturated atmosphere, preventing fruit evaporation and wrinkling, thus delaying ripening. The use of essential oils in the form of a semi-permeable layer also reduces respiration and surface transpiration by inhibiting the movement of oxygen, carbon dioxide, and moisture, playing a positive role in preserving fruit quality.
Conclusion
The aim of this study was to investigate the effect of alginate coating and Shirazi thyme essential oil on the post-harvest and physicochemical characteristics, as well as the shelf life of Ber fruit. The results clearly indicated the enhancement of fruit quality using edible coatings compared to the control. The combination of Al + ZEO (0.5%) had significantly positive effects on preserving the postharvest quality of Ber fruit. In fact, by creating a semi-permeable coating on the surface of the fruit, thyme essential oil and alginate lead to limiting the fruit respiration and metabolism and fungi growth, thus delaying the ripening process, increasing TSS and ascorbic acid and total acidity. According to these results, to reduce post- harvest losses and increase productivity of fruit, the use of 0.5% Shirazi thyme essential oil in combination with alginate coating is recommended due to its beneficial effects on the appearance and physicochemical characteristics of Ber fruit during storage.
Acknowledgement
The authors of this article express their gratitude and appreciation for the financial support from the Hormozgan Science and Technology Park and the University of Hormozgan.
Research Article
Food Technology
Behshad Tahani; Babak Beheshti; Mohsen Heidarisoltanabadi; Ehsan Hekmatian
Abstract
Introduction
Isfahan province is considered to be one of the top ten producing provinces of apple orchards with an area of 17,274 hectares and a production of 120,000 tons of apple. Determining the quality parameters of agricultural products requires the use of various methods, which are different according ...
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Introduction
Isfahan province is considered to be one of the top ten producing provinces of apple orchards with an area of 17,274 hectares and a production of 120,000 tons of apple. Determining the quality parameters of agricultural products requires the use of various methods, which are different according to the nature and characteristics of each product. The two main categories for dividing these methods include analytical (objective) methods and sensory (intrinsic) methods. Both qualitative assessment methods can be used in destructive and non-destructive ways. Apple is one of the fruits that can be stored for a relatively long time. Physiological changes during the storage period are inevitable and cause change to some quality characteristics of the fruit, such as firmness, moisture content, density, pH, and soluble solids. Using the methods of determining and extracting the quality characteristics of fruit during storage and monitoring the changes created, provides a suitable criterion for selecting and preparing the necessary conditions for storage and storage at the disposal of users and producers. X-ray imaging technique is among the methods used and computed tomography, which is now well-known in evaluation of various agricultural commodities and food quality. X-rays are electromagnetic waves with a short wavelength (0.01 to 10 nm) and high energy (from 120 electron volts to 120 kilo electron volts) that can penetrate many materials.
Materials and Methods
In order to determine the relationship between the physicochemical properties and the CT number of Golden Delicious apples, 280 Golden Delicious apples were collected from an orchard located in Semirom city of Isfahan province and stored in a cold room at 0 and 4 ºC and a humidity of 85±5%. These apples were harvested and collected from several trees after the fruit ripened completely. The weight and volume of apples were randomly selected based on the apples on the trees. The samples of stored apples were examined in 4 stages and based on the duration of storage on zero (the beginning of storage), 45, 90 and 135 days.. These tests included non-destructive tests of CT scan imaging and destructive tests to extract the physicochemical properties of apples, including CT number, pH, firmness, moisture, density, and soluble solids. In order to prepare CT scan images of apple samples (non-destructive X-ray test), a GE Healthcare multi-slice CT scan machine model 5122080-12 with 80 kV and 10 mA was used in the CT scan center of Al-Zahra Hospital in Isfahan. Moisture content was determined by weighing the samples and recording their moisture changes before and after drying in the oven at 105 ºC s for 24 hours. Total soluble solids were measured by refractometer device and reported as Brix degree. In order to measure the density of fruits, the weight and volume of each fruit was recorded. A digital pH meter was used to measure the pH of fruit. Penetrometer device was used to measure the firmness of apples. At the end, with the specified values, Pearson correlation coefficients between CT number and other physicochemical properties were determined. Also, by plotting the mentioned values, the most suitable regression equations with the highest coefficients of determination were obtained.
Results and Discussion
Based on the results, the average value of CT number, pH, firmness, moisture, density and total soluble solid of apple at two temperatures of zero and 4 ºC were -115.02 and -166.86, 3.85 and 3.86, 76 37.36 and 33.36 N, 0.82 and 0.80%, 0.76 and 0.72 g/cm3 and 15.30 and 15.79% Brix, respectively. The results showed that the CT number has a negative linear correlation with pH and total soluble solid and a positive linear correlation with the properties of apple firmness, moisture and fruit density. Based on the results, at two storage temperatures of 0 and 4 ºC, the coefficients of determination (R2) obtained from the linear regression model of CT number with pH levels of 0.75 and 0.55, apple firmness 0.32 and 0.57, moisture content 0.78 and 0.85, fruit density 0.82 and 0.84 and total soluble solid 0.85 and 0.62 were obtained.
Conclusion
These results shown that the non-destructive test of X-ray imaging can be used with a suitable approximation to determine some quality properties of apple products.
