Possibility of HAV diagnosis in milk by ELISA technique

Document Type : Research Article

Authors

Masoud Yavarmanesh ¹

Seyed Ali Mortazavi ¹

Mehdi Nasiri mahalati ²

Javad Barooei ²

¹ Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran.

² Ferdowsi University of Mashhad, Mashhad, Iran.

https://doi.org/10.22067/ifstrj.v1i1.212

Abstract

Food borne viral infections are recognized increasingly as human illneses. Hepatitis A virus (HAV) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk . In this study for approaching to best diagnosis method of HAV in milk by ELISA technique, various dilutions of HAV antigen (1 ml/L, 10-3 ml/L, 10-6 ml/L, 10-9 ml/L) were added to UHT milk . Various treatments for virus(antigen) isolation, showed that milk with acidic coagulation, following filtration with paper and membrane filters, had the most optical density. It`s indicated that this treatment resulted the best diagnosis . Also it has been revealed that this method has not any sensitivity for quantitative determination of HAV(antigen) in milk.

Key words: diagnosis, Hepatitis A virus(HAV), Milk, ELISA

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