نوع مقاله : مقاله پژوهشی

نویسندگان

دانشگاه تربیت مدرس

چکیده

در این تحقیق پروتئین آبکافت از ماهی ساردین پهلو طلایی (Sardinella gibossa) با استفاده از آنزیم پاپائین تولید گردید و شرایط آبکافت (زمان, دما و فعالیت آنزیم) جهت حصول به خاصیت آنتی اکسیدانی مطلوب با استفاده از روش سطح پاسخ بهینه سازی شد. ضریب تعیین کلی، ضریب تعیین اصلاح شده و ضریب دقت محاسبه شده توانایی تخمین مدل را نشان داد. شرایط بهینه این آزمایش عبارت بود از غلظت آنزیم 2% , زمان30 دقیقه و دمای 45 درجه سانتی گراد که در نتیجه بر اساس چنین شرایطی میزان حذف رادیکال آزاد DPPH برابر 5/64 درصد بدست آمد. بر اساس نتایج آنالیز اسید آمینه و شاخص شیمیایی متیونین و ایزولوسین اسیدهای آمینه محدود کننده بودند اما دیگر اسیدهای آمینه به میزان لازم وجود داشتند. در نتیجه گیری نهایی می‌توان گفت که مدل حاصل از شرایط خوبی جهت پیش بینی برخوردار است و پروتئین آبکافت ماهی ساردین پهلو طلایی دارای خواص آنتی اکسیدانی مطلوبی در سطح بهینه می‌باشد و می‌توان از این فرآورده شیلاتی جهت استفاده به عنوان مکمل غذایی استفاده نمود.

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