Evaluation of antimicrobial effect of Hop water extract with Humulune from Hop against Staphylococcus aureus, Enterobacter aerogenes, Listeria monocytogenes and Salmonella enterica

Document Type : Short Article

Authors

Ali Mohamadi Sani

Mryam Azami

Masoud Yavarmanesh

https://doi.org/10.22067/ifstrj.v1394i2.38741

Abstract

Plants derived products have been used for medicinal purposes for centuries because of antimicrobial activity. In this investigation Antibacterial effect of Hop(Humulus lupulus), the pharmaceutical plant which has an important place in traditional medicine of Iran, was evaluated on some of food borne pathogens .This study conducted in order to evaluate antimicrobial effect of Hop water extract compared to Humulune from Hop against Staphylococcus aureus (PTTC1431), Enterobacter aerogenes (PTCC1221), Listeria monocytogenes (PTCC1298) and Salmonella enterica (PTCC1709) by agar disk diffusion and broth microdilution methods. The results indicated that gram positive bacteria were more sensitive to the extracts compared to gram negative bacteria. The minimum inhibitory concentration (MIC) of Humulune and water extract of Hop against Staphylococcus aureus were 0.039 and 0.625 mg/ml respectively. Findings indicate that Humulune had the most antibacterial activity in both methods. The most inhibitory effect was shown against staphylococcus aureus in disk diffusion method (inhibition zone of 17mm). The effect of Humulune were more than water extract of Hop. Results indicate that Hop extract has antibacterial effect and can be used in combinational therapy or as a food preservative

Keywords

Humulus lupulus

Minimum inhibitory concentration

water extract

Humulune

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