The Effect of Different Concentrations of Glycerol on Properties of Blend Films Based on Polyvinyl Alcohol-AllysumHomolocarpum Seed Gum

Document Type : Research Article

Authors

• Leila Monjazeb Marvdashti
• Masoud Yavarmanesh
• Arash Koocheki

Ferdowsi university of Mashhad

https://doi.org/10.22067/ifstrj.v12i5.53473

Abstract

Introduction:Packaging is an important factor in food industry and is dominated by petroleum-derived polymers. Therefore, the amount of research involving the production and characterization of biodegradable films has increased substantially, mainly due to interest in minimizing the ecological impact caused by the use of synthetic packaging materials. Several biopolymers have been exploited to develop eco-friendly food packaging materials. Usually, films based on biopolymers are highly sensitive to environmental conditions and generally present low mechanical resistance. As a result, several researchers have developed films based on mixtures of biopolymers and synthetic polymers. In order to increase the workability and flexibility of biodegradable films, various plasticizers, usually poly-ols, have been widely used, glycerol being one of the most preferred and most studied. Plasticizers reduce intermolecular forces, increase the mobility of the biopolymer chains and thereby improve the mechanical properties of the films. Therefore, the aim of the present study were to investigate the effect of different proportions (20e100% w/w) of plasticizer (glycerol) on physicochemical, mechanical, permeability, surface and thermal properties of biodegradable PVA-AHSG blend films.
Polymer blending is one of the most effective methods to have new material with desired properties. Films formed by blending of polymers usually results in modified physical and mechanical properties compared to films made of individual components. Since synthetic polymers are easily obtained and have low production cost, blending of natural and synthetic polymers improves the cost performance ratio of the resulting films.
Since Alyssum homolocarpumseed gum (AHSG) is environmentally friendly due to its biodegradability and has good film forming properties, it is considered as a very promising biopolymer.
Some synthetic polymers from non-renewable sources are also biodegradable, such as polyvinyl alcohol (PVA). PVA is a synthetic, water soluble polymer with excellent film forming, emulsifying, and adhesive properties. It also imparts good tensile strength (TS) and biodegradability and hence has been used in many biomaterial applications. PVA has also been approved for use in packaging meat and poultry products by the USDA (DeMerlis&Schonek, 2003). AHSG contains free hydroxyl and amine groups, and is therefore miscible with PVA due to the formation of hydrogen bonds.

Materials and methods: The aim of this study was to investigate the possibility of producing a novel biodegradable blend film from PVA-AHSG with glycerol as plasticizer in the different concentrations. Films were prepared by the casting method using PVA and AHSG (60:40 ratio). Glycerol was used ac plasticizer because it is compatible with PVA-AHSG blend improving film flexibility, facilitating its handling and preventing cracks. The PVA–AHSG blend film was prepared with different glycerol concentration (20–70%, w/w).The optical properties such as opacity and color were measured. Water vapor permeability, moisture content, water solubility and density of the films were also investigated. Films were evaluated for mechanical and antitoxin properties. The PVA–AHSG blend films were characterized using DSC, FTIR and scanning electron microscopy.

Results and Discussion: The results of this study showed that blend of PVA and AHSG could be used as a new film-forming material. However, it was not possible to make PVA-AHSG blend films without addition of glycerol as a plasticizer to the formula. Glycerols in 20-70% (w/w) concentration were used to prepare the blend films. At the level of 20% (W/W) of glycerol, PVA-AHSG blend films had the lowest thickness (0.065 mm), moisture sorption (118.76%), water vapor permeability (WVP) values (4.9 g mm m-2 kPa−1 d-1), elongation at break (EB)(2.1%), moisture content (22.5%) and water solubility (16.6%) and the highest values for tensile strength (TS)(64.6 MPa), young modulus (YM) (892 MPa),density (0.109 g cm-3),opacity (0.069 A/mm) and water contact angle (74.52◦). Increasing of glycerol concentration in PVA-AHSG blend films resulted in increase in water vapor permeability and percent of elongation while, decreased tensile strength and surface hydrophobicity.
Increasing the glycerol concentration significantly (p < 0.05) diminished initial water contact angle of films from 74.52◦ to 37.80◦. It has been shown that the addition of plasticizers diminished the films’ water contact angle, which in turn, decreased hydrophobicity of the films. The higher hydro-philicity of the samples is attributable to the hygro-scopicity (water-binding capacity) of the plasticizer. Plasticizer can diminish interactions between biopolymer molecules and increase solubility due to its hydrophilic nature, giving the polymer molecules higher affinity to attract water. The moisture content increased significantly from 22.5% to 40.9% as the plasticizer content increased (p < 0.05). Because of glycerol acts as a water-holding agent, with the higher number of water molecules in glycerol-plasticized films increasing plasticizing activity.WVP increases as plasticizer content of the film increases due to its hydrophilic nature. WVP can be directly related to the quantity of OH group on the molecule. Also, environmental conditions can significantly affect the WVP. Increasing plasticizer concentration decreased the intermolecular forces between polymer chains and increased free volume and segmental motions, allowing water molecules to diffuse more easily and giving a higher WVP. Mechanical strength of films decreases due to plasticizer addition resulting in decreased tensile strength and increased elongation. The measurement of color values showed that by the increasing of the glycerol concentration in polymers blend matrix, the b and L values increased while a value decreased. Furthermore, the addition of glycerol promoted the interactions among PVA, AHSG and glycerol through hydrogen bonding as reflected on the shifting of main peaks of the glycerol-free film to higher wavenumbers as shown by FTIR spectra. Microscopic views indicated smooth and uniform surface morphology without obvious cracks, breaks, or openings on the surfaces after the incorporation of glycerol as a plasticizer. Scanning electron microscopy showed that the microstructure of PVA-AHSG blend films have a critical effect on their physical and mechanical properties that is important in food packaging applications.

Keywords

• Polyvinyl alcohol
• Alyssum homolocarpum seeds gum
• Glycerol
• Biodegradable film

References

Abdollahi, M., Alboofetileh, M., Rezaei, M., & Behrooz, R, (2013). Comparing physico-mechanical and thermal properties of alginate nanocomposite films reinforced with organic and/or inorganic nanofillers. Food Hydrocolloids, 32(2), 416-424.

Abugoch, L. E., Tapia, C., Villaman, M. C., Yazdani-Pedram, M., & Diaz-Dosque, M, (2011). Characterization of quinoa protein–chitosan blend edible films. Food Hydrocolloids, 25(5), 879-886.

Ahmadi, R., Kalbasi-Ashtari, A., Oromiehie, A., Yarmand, M.-S., & Jahandideh, F, (2012). Development and characterization of a novel biodegradable edible film obtained from psyllium seed (Plantago ovata Forsk). Journal of Food Engineering, 109(4), 745-751.

Angles, M. N., & Dufresne, A, (2000). Plasticized starch/tunicin whiskers nanocomposites. 1. Structural analysis. Macromolecules, 33(22), 8344-8353.

Antoniou, J., Liu, F., Majeed, H., Qazi, H. J., & Zhong, F, (2014). Physicochemical and thermomechanical characterization of tara gum edible films: effect of polyols as plasticizers. Carbohydrate polymers, 111, 359-365.

Arismendi, C., Chillo, S., Conte, A., Del Nobile, M. A., Flores, S., & Gerschenson, L. N, (2013). Optimization of physical properties of xanthan gum/tapioca starch edible matrices containing potassium sorbate and evaluation of its antimicrobial effectiveness. LWT-Food Science and Technology, 53(1), 290-296.

ASTM, Designation E96-00, (2000). Standard method for water Vapor transmission of materials. In Annual Book of ASTM Standards. American Society for Testing and Materials.

ASTM, Designation D882-02, (2002). Standard method for Tensile Properties of Thin Plastic Sheeting. In Annual Book of ASTM Standards. American Society for Testing and Materials.

da Silva, M. A., Bierhalz, A. C. K., & Kieckbusch, T. G, (2009). Alginate and pectin composite films crosslinked with Ca 2+ ions: effect of the plasticizer concentration. Carbohydrate polymers, 77(4), 736-742.

Ebrahimi, S. E., Koocheki, A., Milani, E., & Mohebbi, M, (2015). Interactions between Lepidium perfoliatum seed gum–grass pea (Lathyrus sativus) protein isolate in composite biodegradable film. Food Hydrocolloids.

Fabra, M., Talens, P., & Chiralt, A, (2010). Water sorption isotherms and phase transitions of sodium caseinate–lipid films as affected by lipid interactions. Food Hydrocolloids, 24(4), 384-391.

Garcia, M. A., Pinotti, A., & Zaritzky, N. E, (2006). Physicochemical, water vapor barrier and mechanical properties of corn starch and chitosan composite films. Starch‐Stärke, 58(9), 453-463.

Gennadios, A., Weller, C., Hanna, M., & Froning, G, (1996). Mechanical and barrier properties of egg albumen films. Journal of food science, 61(3), 585-589.

Ghanbarzadeh, B., Almasi, H., & Entezami, A. A, (2010). Physical properties of edible modified starch/carboxymethyl cellulose films. Innovative food science & emerging technologies, 11(4), 697-702.

Ghasemlou, M., Khodaiyan, F., & Oromiehie, A, (2011). Physical, mechanical, barrier, and thermal properties of polyol-plasticized biodegradable edible film made from kefiran. Carbohydrate polymers, 84(1), 477-483.

Gontard, N., & Guilbert, S, (1994). Bio-packaging: technology and properties of edible and/or biodegradable material of agricultural origin Food packaging and preservation (pp. 159-181): Springer.

Gontard, N., Guilbert, S., & Cuq, J. L, (1993). Water and glycerol as plasticizers affect mechanical and water vapor barrier properties of an edible wheat gluten film. Journal of food science, 58(1), 206-211.

Gupta, B., Agarwal, R., & Sarwar Alam, M. S. (2013). Preparation and characteri-zation of polyvinyl alcohol–polyethylene oxide–carboxymethyl cellulose blendmembranes. Journal of Applied Polymer Science, 127(2), 1301–1308.

Han, J. H., & Floros, J. D, (1997). Casting antimicrobial packaging films and measuring their physical properties and antimicrobial activity. Journal of Plastic Film and Sheeting, 13(4), 287-298.

Haq, M. A., Hasnain, A., & Azam, M, (2014). Characterization of edible gum cordia film: Effects of plasticizers. LWT-Food Science and Technology. 55(1), 163-169.

Hesarinejad, M. A., Razavi, S. M., & Koocheki, A, (2015). Alyssum homolocarpum seed gum: Dilute solution and some physicochemical properties. International journal of biological macromolecules, 81, 418-426.

Jia, D., Fang, Y., & Yao, k, (2009). Water vapor barrier and mechanical properties of konjac glucomannan–chitosan–soy protein isolate edible films. Food and bioproducts processing, 87(1), 7-10.

Jost, V., Kobsik, K., Schmid, M., & Noller, K, (2014). Influence of plasticiser on the barrier, mechanical and grease resistance properties of alginate cast films. Carbohydrate polymers, 110, 309-319.

Jouki, M., Khazaei, N., Ghasemlou, M., & HadiNezhad, M, (2013a). Effect of glycerol concentration on edible film production from cress seed carbohydrate gum. Carbohydrate polymers, 96(1), 39-46.

Jouki, M., Yazdi, F. T., Mortazavi, S. A., & Koocheki, A, (2013b). Physical, barrier and antioxidant properties of a novel plasticized edible film from quince seed mucilage. International journal of biological macromolecules, 62, 500-507.

Kanatt, S. R., Rao, M., Chawla, S., & Sharma, A, (2012). Active chitosan–polyvinyl alcohol films with natural extracts. Food Hydrocolloids, 29(2), 290-297.

Khazaei, N., Esmaiili, M., Djomeh, Z. E., Ghasemlou, M., & Jouki, M, (2014). Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum. Carbohydrate polymers, 102, 199-206.

Koocheki, A., Mortazavi, S. A., Shahidi, F., Razavi, S., Kadkhodaee, R., & Milani, J. M, (2010). Optimization of mucilage extraction from Qodume shirazi seed (Alyssum homolocarpum) using response surface methodology. Journal of Food Process Engineering, 33(5), 861-882.

Koocheki, A., Shahidi, F., Mortazavi, S.A., Karimi, M., & Milani, E. (2011). Effect of QodumeShirazi (Alyssum Homolocarpum) seed and xanthan gum on rheological properties of wheat flour dough and quality of bread.Iranian Food Science and Technology Research Journal. 7(1): 9-16.

Kowalczyk, D., & Baraniak, B, (2011). Effects of plasticizers, pH and heating of film-forming solution on the properties of pea protein isolate films. Journal of Food Engineering, 105(2), 295-305.

Kurt, A., & Kahyaoglu, T. (2014), Characterization of a new biodegradable edible film made from salep glucomannan. Carbohydrate polymers, 104, 50-58.

Li, Y., Jiang, Y., Liu, F., Ren, F., Zhao, G., & Leng, X, (2011). Fabrication and characterization of TiO 2/whey protein isolate nanocomposite film. Food Hydrocolloids, 25(5), 1098-1104.

Mali, S., Sakanaka, L., Yamashita, F., & Grossmann, M, (2005). Water sorption and mechanical properties of cassava starch films and their relation to plasticizing effect. Carbohydrate polymers, 60(3), 283-289.

Marsh, K., & Bugusu, B, (2007). Food packaging—roles, materials, and environmental issues. Journal of food science, 72(3), R39-R55.

Martins, J. T., Cerqueira, M. A., Bourbon, A. I., Pinheiro, A. C., Souza, B. W., & Vicente, A. A, (2012). Synergistic effects between κ-carrageenan and locust bean gum on physicochemical properties of edible films made thereof. Food Hydrocolloids, 29(2), 280-289.

Mchugh, T. H., Aujard, J. F., & Krochta, J, (1994). Plasticized whey protein edible films: water vapor permeability properties. Journal of food science, 59(2), 416-419.

Motedayen, A. A., Khodaiyan, F., & Salehi, E. A. (2013). Development and characterisation of composite films made of kefiran and starch. Food chemistry, 136(3), 1231-1238.

Mu, C., Guo, J., Li, X., Lin, W., & Li, D, (2012). Preparation and properties of dialdehyde carboxymethyl cellulose crosslinked gelatin edible films. Food Hydrocolloids, 27(1), 22-29.

Müller, C. M., Yamashita, F., & Laurindo, J. B, (2008). Evaluation of the effects of glycerol and sorbitol concentration and water activity on the water barrier properties of cassava starch films through a solubility approach. Carbohydrate polymers, 72(1), 82-87.

Muñoz, L., Aguilera, J., Rodriguez-Turienzo, L., Cobos, A., & Diaz, O, (2012). Characterization and microstructure of films made from mucilage of Salvia hispanica and whey protein concentrate. Journal of Food Engineering, 111(3), 511-518.

Nkwachukwu, O. I., Chima, H. C., Ikenna, A. O., & Albert, L. (2013). Focus on potential environmental issues on plastic world towards a sustainable plastic recycling in developing countries. International Journal of Industrial Chemistry, 4, 1-34.

Piermaria, J., Bosch, A., Pinotti, A., Yantorno, O., Garcia, M. A., & Abraham, A. G, (2011). Kefiran films plasticized with sugars and polyols: water vapor barrier and mechanical properties in relation to their microstructure analyzed by ATR/FT-IR spectroscopy. Food Hydrocolloids, 25(5), 1261-126.

Piermaria, J. A., Pinotti, A., Garcia, M. A., & Abraham, A. G, (2009). Films based on kefiran, an exopolysaccharide obtained from kefir grain: Development and characterization. Food Hydrocolloids, 23(3), 684-690.

Ramos, Ó. L., Reinas, I., Silva, S. I., Fernandes, J. C., Cerqueira, M. A., Pereira, R. N., & Malcata, F. X, (2013). Effect of whey protein purity and glycerol content upon physical properties of edible films manufactured therefrom. Food Hydrocolloids, 30(1), 110-122.

Razavi, S. M. A., Amini, A. M., & Zahedi, Y. (2015). Characterisation of a new biodegradable edible film based on sage seed gum: Influence of plasticiser type and concentration. Food Hydrocolloids, 43, 290-298.

Rezaee Taghiabadi, M., Maftoonazad, N., Badiee, F., & Hosseini, SA. A. (2013). Evaluation of factors affecting mechanical properties, opacity and water vapor permeability of edible films based on gum tragacanth by using RSM method. Journal of Food Science, 9 (37), 123 to 134.

Saurabh, C. K., Gupta, S., Bahadur, J., Mazumder, S., Variyar, P. S., & Sharma, A, (2013). Radiation dose dependent change in physiochemical, mechanical and barrier properties of guar gum based films. Carbohydrate polymers, 98(2), 1610-1617.

Seyedi, S., Koocheki, A., Mohebbi, M., & Zahedi, Y, (2014). Lepidium perfoliatum seed gum: A new source of carbohydrate to make a biodegradable film. Carbohydrate polymers, 101, 349-358.

Shen, Z., Ghasemlu, M., & Kamdem, D. P. (2015). Development and compatibility assessment of new composite film based on sugar beet pulp and polyvinyl alcohol intended for packaging applications. Journal of applied polymer science,132, 41354.

Silva, F. E. F., Batista, K. A., Di-Medeiros, M. C. B., Silva, C. N. S., Moreira, R. B., & Fernandes, K. F. (2016). A stimuli-responsive and bioactive film based on blended polyvinyl alcohol and cashew gum polysaccharide. Materials Science and Engineering, 58, 927–934.

Sothornvit, R., & Krochta, J, (2005). Plasticizers in edible films and coatings. Innovations in food packaging, 403-433.

Sothornvit, R., & Krochta, J. M, (2001). Plasticizer effect on mechanical properties of β-lactoglobulin films. Journal of Food Engineering, 50(3), 149-155.

Souza, B. W., Cerqueira, M. A., Teixeira, J. A., & Vicente, A. A, (2010). The use of electric fields for edible coatings and films development and production: a review. Food Engineering Reviews, 2(4), 244-255.

Srinivasa, P., Ramesh, M., Kumar, K., & Tharanathan, R, (2003). Properties and sorption studies of chitosan–polyvinyl alcohol blend films. Carbohydrate polymers, 53(4), 431-438.

Su, J.-F., Huang, Z., Yuan, X.-Y., Wang, X.-Y., & Li, M, (2010). Structure and properties of carboxymethyl cellulose/soy protein isolate blend edible films crosslinked by Maillard reactions. Carbohydrate polymers, 79(1), 145-153.

Sun, Q., Sun, C., & Xiong, L, (2013). Mechanical, barrier and morphological properties of pea starch and peanut protein isolate blend films. Carbohydrate polymers, 98(1), 630-637.

Suyatma, N. E., Tighzert, L., Copinet, A., & Coma, V, (2005). Effects of hydrophilic plasticizers on mechanical, thermal, and surface properties of chitosan films. Journal of Agricultural and Food Chemistry, 53(10), 3950-3957.

Synytsya, A., Čopı́kova, J., Matějka, P., & Machovič, V, (2003). Fourier transform Raman and infrared spectroscopy of pectins. Carbohydrate polymers, 54(1), 97-106.

Thakhiew, W., Devahastin, S., & Soponronnarit, S, (2010). Effects of drying methods and plasticizer concentration on some physical and mechanical properties of edible chitosan films. Journal of Food Engineering, 99(2), 216-224.

Tian, H., Xu, G., Yang, B., & Guo, G, (2011). Microstructure and mechanical properties of soy protein/agar blend films: Effect of composition and processing methods. Journal of Food Engineering, 107(1), 21-26.

Wilhelm, H.-M., Sierakowski, M.-R., Souza, G., & Wypych, F. (2003). Starch films reinforced with mineral clay. Carbohydrate polymers, 52(2), 101-110.

Yang, S. Y., & Huang, C. Y. (2008). Plasma treatment for enhancing mechanical andthermal properties of biodegradable PVA/starch blends. Journal of Applied Poly-mer Science, 109(4), 2452–2459.

Yuen, S.-N., Choi, S.-M., Phillips, D. L., & Ma, C.-Y. (2009). Raman and FTIR spectroscopic study of carboxymethylated non-starch polysaccharides. Food chemistry, 114(3), 1091-1098.

Zhou, J., Wang, S., & Gunasekaran, S, (2009). Preparation and characterization of whey protein film incorporated with TiO2 nanoparticles. Journal of food science, 74(7), N50-N56