بررسی پایداری، رنگ و ویسکوزیته ی نانوکمپلکس های پروتئین آب پنیر – پکتین به عنوان نانوحامل روغن پوست پرتقال

نوع مقاله : مقاله پژوهشی

نویسندگان

• ساناز قاسمی ¹
• سید مهدی جعفری ¹
• مرتضی خمیری ¹
• الهام اسدپور ²

¹ گروه علوم و صنایع غذایی، دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی، گرگان، ایران.

² گروه علوم و صنایع غذایی، موسسه آموزش عالی بهاران، گرگان، ایران.

https://doi.org/10.22067/ifstrj.v1396i0.62600

چکیده

روغن پوست پرتقال، طعم دهنده ی پرمصرف صنعتی، در مجاورت هوا، نور، رطوبت و دمای بالا فرار و از نظر شیمیایی ناپایدار می باشد. نانوکمپلکس های بیوپلیمری، دسته ای از نانو حامل هایی هستند که بیشتر با استفاده از برهم کنش های الکترواستاتیک بین گروه های باردار پلی ساکاریدها و پروتئین ها تولید می شوند. این نانوذرات توانایی حمل، حفاظت و افزایش زیست دسترسی ترکیبات غذا- دارو را دارند. از این رو در این پژوهش، نانوکمپلکس های بیوپلیمری پروتئین آب پنیر- پکتین به عنوان حامل روغن پوست پرتقال تولید شد و ویژگی های محلول نانوکمپلکس تهیه شده با غلظت های مختلف پروتئین آب پنیر (4، 6 و 8 %)، پکتین (5/0، 75/0 و 1 %) و مقادیر مختلف pH (3، 6 و 9) مورد بررسی قرار گرفت. ویسکوزیته، پایداری و رنگ (شاخصL*) تیمارهای طراحی شده، به روش سطح پاسخ بررسی شد. نتایج نشان داد پروتئین آب پنیر 4% و پکتین 1% در 3pH= و 9 pH= به ترتیب کمترین و بیشترین پایداری را دارا بودند. همچنین این غلظت ها در 3pH= بیشترین ویسکوزیته و بیشترین L* را نیز نشان دادند. کمترین ویسکوزیته در پروتئین آب پنیر 6% و پکتین 75/0% در 3pH= بدست آمد که به دلیل نامتعادل بودن غلظت ها، کمپلکسی تشکیل نشد و جدایی فازها رخ داد. کمترین L* را نیز پروتئین آب پنیر4% و پکتین 5/0% در 9 pH= داشته است. در نهایت پروتئین آب پنیر 4% و پکتین 1% در3pH= به دلیل تشکیل کمپلکس قوی و مناسب، به عنوان نمونه ی بهینه انتخاب شد. اندازه ی ذرات و پتانسیل زتای نمونه ی بهینه به ترتیب nm 160 و mV53/0- بدست آمد.

کلیدواژه‌ها

• نانوحامل
• کمپلکس
• پروتئین آب پنیر
• روغن پوست پرتقال
• پکتین

عنوان مقاله [English]

Evaluation of the stability, color, and viscosity for whey protein-pectin nano complexes as nano carriers of orange peel oil

نویسندگان [English]

• Sanaz Ghassemi ¹
• Seyed Mahdi Jafari ¹
• Morteza Khomeiri ¹
• Elham Assadpour ²

¹ Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Iran.

² Department of Food Science and Technology, Baharan Institute of Higher Education, Gorgan, Iran.

چکیده [English]

Orange peel oil,a widely used industrial flavoring, is volatile and chemically unstable in the presence of air, light, moisture and high temperatures. Biopolymer Nano complexes, a bunch of Nano carriers, are produced between groups of charged polysaccharides and proteins with the use of electrostatic interactions. The nanoparticles are able to carry, protect, and increase their bioavailability of food-drug materials. Hence, in this study, the Nano complexes of pectin- whey protein concentrate, as a carrier of orange peel oil, were produced and the features of prepared Nano complex solution with various concentrations of whey protein (4, 6 and 8%), pectin (0.5, 0.75 and 1%) and different values of pH (3, 6 and 9) were studied. The Viscosity, stability and color (index L *) of the treated designs, done in a response surface methodology, were examined. The results showed that whey protein 4% and pectin 1%( with a pH of 3 and 9) had the lowest and highest stability respectively. Also this treatment with a pH of 3 showed the highest viscosity as well as the highest L *. The lowest viscosity was achieved by whey protein 6% and pectin 0.75% with a pH of 3, and because of the unbalanced compound, the complex did not form and a phase separation occurred. The lowest L * was obtained by whey protein 4% and pectin 0.5% with a PH of 9. Ultimately, whey protein 4% and pectin 1% with a PH of 3 were selected as the optimum sample because of formation the strong and suitable complex. Particle size and zeta potential measurement of optimum sample, were 160 nm and -0.53 mV respectively.

کلیدواژه‌ها [English]

• nano carrier
• Complex
• whey protein
• pectin
• orange peel oil

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