Document Type : Full Research Paper


1 Department of Processing of Fishery Products, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, 09113964324, P. O. Box :46511-66289.

2 Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, 01133687574, P. O. Box: 4818166996.


Introduction: Shrimps are highly sensitive to oxidation at refrigerator temperature. On the other hand, storage of shrimp in freezing conditions leads to a decrease in product quality after thawing. It should be noted that shrimp oxidation also occurs in freezing conditions, but the oxidation rate in these conditions is much slower than storage in refrigerated conditions. Therefore, it seems necessary to use a method that can control the oxidation of shrimp in both freezing and refrigerating conditions. The aim of this study was to evaluate the feasibility of controlling shrimp oxidation (at refrigerator temperature) using whey protein coating containing ascorbic acid or α-tocopherol, and to compare the efficacy of these antioxidants (in combination with whey protein).
Materials and Methods: In order to advance the purpose of the research, shrimp fillets were stored in four treatments, including treatments No. 1 (control), 2 (shrimp fillet coated by whey protein), 3 (shrimp fillet coated by whey protein + ascorbic acid) and 4 (shrimp fillet coated by whey protein+ α-tocopherol) at refrigerator temperature for 9 days.  In order to evaluate the oxidation intensity and also the stability of the treatments against oxidative damage, peroxide indices, free fatty acids, anisidine and thiobarbituric acid of the treatments were determined on days 0, 3, 6 and 9. This study was implemented in form of completely randomized design and data were analyzed by one-way ANOVA. Significant differences among means were tested by Duncan's test at 95 confidence level.
Results and Discussion: The results showed that whey protein alone (treatment 2) as shrimp coating can partially control the oxidation process of fillet fats compared to control. But when whey protein was combined with ascorbic acid (treatment 3) and α-tocopherol (treatment 4), the coatings' strength against oxidative deterioration significantly increased (p<0.05). According to our findings, during the storage period, the lowest amount of peroxide, free fatty acids, anisidine and thiobarbituric acid indices were related to treatment 3 (p<0.05). During the storage period, all the mentioned indicators (in all treatments) had an increasing trend, but the slope of this trend was different and the lowest slope was related to treatment 3. Comparison of fresh shrimp fillet fatty acid profile with fatty acid profile of treatments at day 9 showed that the whey protein coating combined with ascorbic acid (treatment 3) had the most protective effect on the structure of fatty acids. Overall, according to the results of the present study, it can be claimed that whey protein- ascorbic acid coating is more effective than whey protein-α-tocopherol coating to increase the oxidative stability of shrimp fillet. Therefore, the ascorbic acid is more efficacious than α -tocopherol (in combination with whey protein) in controlling the oxidation of shrimp fillets.


Main Subjects

  1. Abdel-Aal, H. A. (2001). Using antioxidants for extending the shelf life of frozen Nile karmout (Claries lazera) fish mince. Journal of Aquatic Food Product Technology, 10(4), 87-99.
  2. Ahvenainen, R. (Ed.). (2003). Novel food packaging techniques. Elsevier. CRC Pub. 590pp.
  3. Aubourg, S. P. (1993). Interaction of malondialdehyde with biological molecules—new trends about reactivity and significance. International journal of food science & technology, 28(4), 323-335.
  4. Aubourg, S. P., Pérez‐Alonso, F., & Gallardo, J. M. (2004). Studies on rancidity inhibition in frozen horse mackerel (Trachurus trachurus) by citric and ascorbic acids. European Journal of Lipid Science and Technology, 106(4), 232-240.
  5. Bhobe, A. M., & Pai, J. S. (1986). Study of the properties of frozen shrimps. Journal of food science and technology, 23(3), 143-147.
  6. Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37(8), 911-917.
  7. Boonsumrej, S., Chaiwanichsiri, S., Tantratian, S., Suzuki, T., & Takai, R. (2007). Effects of freezing and thawing on the quality changes of tiger shrimp (Penaeus monodon) frozen by air-blast and cryogenic freezing. Journal of Food Engineering, 80(1), 292-299.
  8. Egan, H., & Sawyer, R. (1997). Pearson's chemical Analysis of food. 9th. Edition, Edinburgh, Scotland, Churchill. Livingstone, UK, 609-634.
  9. Farshidi, M., Ehsani, A., Ebrahimi, B., Bahar Banafshe, M., & Nazari, GH. (2017). Evaluation of whey protein coating efficacy on shrimp fillet quality under cold conditions. Journal of Food Processing and production, 7 (3), 37-45
  10. Georgantelis, D., Ambrosiadis, I., Katikou, P., Blekas, G., & Georgakis, S. A. (2007). Effect of rosemary extract, chitosan and α-tocopherol on microbiological parameters and lipid oxidation of fresh pork sausages stored at 4 C. Meat science, 76(1), 172-181.
  11. Gonçalves, A. A., & Ribeiro, J. L. D. (2008). Optimization of the freezing process of red shrimp (Pleoticus muelleri) previously treated with phosphates. International Journal of Refrigeration, 31(7), 1134-1144.
  12. Gounga, M. E., Xu, S. Y., & Wang, Z. (2007). Whey protein isolate-based edible films as affected by protein concentration, glycerol ratio and pullulan addition in film formation. Journal of Food Engineering83(4), 521-530.
  13. Han, C., Zhao, Y., Leonard, S. W., & Traber, M. G. (2004). Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria ananassa) and raspberries (Rubus ideaus). Postharvest Biology and Technology, 33(1), 67-78.
  14. Hoseini, M. & Milani, J. (2016). Increasing oxidative stability of walnut kernel by whey protein isolate coating. Journal of Food Industry Research, 27 (3), 91-102
  15. Hui, Y. H., Legarretta, I. G., Lim, M. H., Murrell, K. D., & Nip, W. K. (Eds.). (2004). Handbook of frozen foods(Vol. 133). CRC Press.
  16. Kanner, J. (2007). Dietary advanced lipid oxidation endproducts are risk factors to human health. Molecular Nutrition and Food research, 51(9), 1094-1101.
  17. Khezri, M., Rezaei, M., & Ojagh, M. (2012). The effect of ascorbic acid combined with whey protein coating on the shelf-life of rainbow trout stored at refrigerator temperature: Microbial and chemical analyzes. Iranian Journal of Nutrition Sciences & Food Technology, 7 (3), 69-78
  18. Khosravi, M., Matini, S., & Jalilzadeh, A. (2019). The effect of edible coating based on whey protein containing black seed extract on the shelf life of perch fillet in refrigerated conditions. The 3rd International Congress and the 26th National Congress of Food Science and Technology of Iran, Tehran.
  19. Kirk, R.S. & Sawyer, R. (1991). Pearsons chemical analysis of foods. (9th Ed.) Longman Scientific and Technical. Harlow, Essex, UK.
  20. Lakshmanan, P. T. (2000). Fish spoilage and quality assessment. In T. S. G. Iyer, M. K. Kandoran, Mary Thomas, & P. T. Mathew (Eds.), Quality assurance in seafood processing, pp. 26– Cochin: Society Fisher Techno (India).
  21. Lin, C. C., & Lin, C. S. (2005). Enhancement of the storage quality of frozen bonito fillets by glazing with tea extracts. Food control, 16(2), 169-175.
  22. Mielnik, M. B., Aaby, K., & Skrede, G. (2003). Commercial antioxidants control lipid oxidation in mechanically deboned turkey meat. Meat science, 65(3), 1147-1155.
  23. Min, S., & Krochta, J. M. (2007). Ascorbic acid-containing whey protein film coatings for control of oxidation. Journal of agricultural and food chemistry, 55(8), 2964-2969.
  24. Mottalebi, A., Hasanzati, A., Khanipour, A., & Soltani, M. (2012). Effect of whey protein edible coating on moisture and sensory characteristics of empty stomach kilka. Journal of Food Science and Nutrition, 9 (4), 39-47
  25. Murphy, R. C. (1993). Mass spectrometry of lipids. Plenum press. 290 p.
  26. Naturland V. (2002). Organic farming in the tropics and subtropics exemplary description of 20 crops:  cashew nuts. 1 st ed., Germany.
  27. Ozdemir, M., & Floros, J. D. (2008). Optimization of edible whey protein films containing preservatives for mechanical and optical properties. Journal of Food Engineering, 84(1), 116-123.
  28. Paktermani, M., Ehsani, E., & Ghajarbeygi, P. (2017). Investigating -Increase the shelf life of fish with Edible Alginate sodium-Based Film containing α-tocopherol. Food Science and Technology, 61(13), 17-24.
  29. P-Anisidine Value. Cd 18-90: AOCS (Official Methods and Recommended Practices of the AOCS).
  30. Parvaneh, v. (2019). Quality control and food chemical testing. University of Tehran Press, 335p
  31. Pazos, M., Gallardo, J. M., Torres, J. L., & Medina, I. (2005). Activity of grape polyphenols as inhibitors of the oxidation of fish lipids and frozen fish muscle. Food Chemistry, 92(3), 547-557.
  32. Pourashouri, P., Shabanpour, B., Aubourg, S. P., Rohi, J. D., & Shabani, A. (2009). An investigation of rancidity inhibition during frozen storage of Wels catfish (Silurus glanis) fillets by previous ascorbic and citric acid treatment. International journal of food science & technology, 44(8), 1503-1509.
  33. Sayyad, M., Alizadeh, E., & Zakipour, E. (2017). Effect of edible Whey protein-monoglyceride coating on the quality of Hypophthalmichthys molitrix fish finger during refrigerated storage. Journal of Food Science and Technology, 64 (14), 93-102
  34. Sayyad, M., Nourzaei, Kh., & Alizadeh, E. (2016). Effects of edible whey protein coating and essential oil of Anethum graveolens on the quality of Hypophthalmichthys molitrix fillet during refrigerated Scientific - Research Journal, 5 (2), 85-94
  35. Seydim, A. C., & Sarikus, G. (2006). Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Research International, 39(5), 639-644.
  36. Seyfzadeh, M. & Mottalebi, A. (2011). The effect of using a combination coating of whey protein and sodium alginate on quality changes of kilka fish during cold storage. Journal of Aquatics and Fisheries, 2 (8), 39-51
  37. Shahin, R., Nayebzadeh, K., Alizadeh, L., & Mohammadi, A. (2014). Antioxidant effect of tocopherol and TBHQ on oil oxidation over the shelf life of mayonnaise. Iranian Journal of Nutrition Sciences & Food Technology, 8 (4), 227-236
  38. Siskos, I., Zotos, A., Melidou, S., & Tsikritzi, R. (2007). The effect of liquid smoking of fillets of trout (Salmo gairdnerii) on sensory, microbiological and chemical changes during chilled storage. Food Chemistry, 101(2), 458-464.
  39. Tarladgis, B. G., Watts, B. M., Younathan, M. T., & Dugan Jr, L. (1960). A distillation method for the quantitative determination of malonaldehyde in rancid foods. Journal of the American Oil Chemists' Society, 37(1), 44-48.
  40. Toluie, H., Mohtadi Niya, J., Arefhosseini, S., & Asghari, M. (2012). The effect of chitosan coating that enriched with α-tocopherol on lipid oxidation in farmed trout (Oncorhynchus mykiss) during refrigerated storage. Journal of Research and Innovation in Food Science and Technology, 1 (3), 153-164
  41. UNECEF Standard, (2002). Concerning the marketing and commercial quality control of cashew kernels, Ddp-17, 2002 ed., United Nations, New York And Geneva.
  42. Whang, K., Aberle, E. D., Judge, M. D., & Peng, I. C. (1986). Antioxidative activity of α-tocopherol in cooked and uncooked ground pork. Meat Science, 17(4), 235-249.
  43. Wolfson, L.M., Sumner, S.S. & Froning, G.W. (1994). Inhibition of Salmonella typhimurium on poultry by the lactoperoxidase system. Food Safety, 14(1), 53-62.
  44. Zuta, P. C., Simpson, B. K., Zhao, X., & Leclerc, L. (2007). The effect of α-tocopherol on the oxidation of mackerel oil. Food Chemistry, 100(2), 800-807.