Document Type : Research Article-en
Authors
MS Graduated student, Department of Food Science and Technology, Damghan Branch, Islamic Azad University, Damghan, Iran
Abstract
Walnuts have a high nutritional value because of their high levels of essential compounds for human health. Phenolic compounds have beneficial properties, including anti-cancer and antimicrobial properties. In this study, the amount of the extracted antioxidants from different parts of walnut, including the walnut kernel, hard shell and green husk by ethanol and water was compared with those of the ultrasound-assisted extraction. This study was performed by determining the amount of polyphenols present and the free radical scavenging power of DPPH. The results showed that the effect of all factors was statistically significant at 99% statistical level. The highest rate of extraction of phenolic compounds (1.09 mg Gallic acid/g) and the highest rate of free radical scavenging of DPPH (6.86%) was related to the use of ethanol solvent for extraction. It was also shown that the hard walnut shell has the highest amount of phenolic compounds (1.1 mg Gallic acid per gram of extract) and the walnut kernel has the highest antioxidant properties (7.99%). Ultrasonic pretreatment increased the extraction efficiency of phenolic compounds and antioxidant properties so that this process increased the extraction of phenolic compounds from the green husk of walnut by 1.22 mg Gallic acid/g and increased the antioxidant properties of the kernel walnut by 13.51% compared to other parts.
Keywords
Main Subjects
- Albu, S., Joyce, E., Paniwnyk, L., Lorimer, J. P. and Mason, T. J. (2004). Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrasonics Sonochemistry, 11(3-4):261–265. https://doi.org/10.1016/j.ultsonch.2004.01.015
- Azadedel, S., Hanachi, P. and Saboora, A. (2018). Investigation on antioxidant activity of pistachio (Pistacia vera L.) skin extraction. Journal of Plant Research (Iranian Journal of Biology), 30(4):722-731.
- Ballard, T. S., Mallikarjunan, P., Zhou, K.& O'Keefe, S. (2010). Microwave-assisted extraction of phenolic antioxidant compounds from peanut skins. Food Chemistry, 120(4): 1185-1192
- Banaian, N. (2010). Estimating the energy of walnut waste and its recycling strategies. National Conference on Agricultural Waste and Waste Management.Tehran, Iran. https://civilica.com/doc/98938
- Chew, K. K., Khoo, M. Z., Ng, S. Y., Thoo, Y. Y. and Aida, W. W. (2011). Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts. International Food Research Journal, 18(4): 1427-1435.
- Chirinos, R., Rogez, H., Campos, D., Pedreschi, R. and Larondelle, Y. (2007). Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers. Separation and Purification Technology, 55(2):217-225. https://doi.org/10.1016/j.seppur.2006.12.005
- Dolat Abadi, M., Raftani Amiri, Z. and Esmailzadeh Kenari, R. (2017a). Comparison of the effect of time and extraction methods on the phenolic compounds and antioxidant properties of walnut green husk of different regions of northern Iran. Iranian Food Science and Technology Research Journal. 13(2):273- 281.
- Dolat Abadi, M., Raftani Amiri, Z. and Esmailzadeh, R. (2017b). Assessment of anti- microbial and anti- oxidant properties of Hezarjerib originated walnut green husk extract in refined soybean oil. Journal of Food Research, 27(4):23-35.
- Dolat Abadi, , Raftani Amiri, Z., Esmail Zadeh Kenari, R. (2014). Comparison of total phenols and antioxidant properties of walnut (Juglans regia L.) green husk of three regions of northern Iran (Shahrood, Bandar Gaz and Hzargarib). Journal of Food Science and Technology (Iran), 11(45):183-192
- FAO. (2020). Retrieved September 27 (2020), http://faostat.fao.org/site/339/default.aspx. Accessed 24 September 2021.
- Fernández- Agulló, A., Castro-Iglesias, A., Freire, M. S. and González-Álvarez, J. (2020). Optimization of the extraction of bioactive compounds from walnut (Juglans major 209 x Juglans regia) leaves: Antioxidant capacity and phenolic profile. Antioxidants, 9(1):18.
- Fernandez- Agullo, A., Pereira, E., Freirea, M. S., Valentao, Andrade, P. B., Gonzalez-Alvareza, and Pereira, J. A. (2013). Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia) green husk extracts. Industrial Crops and Products, 42(2013):126– 132. https://doi.org/10.1016/j.indcrop.2012.05.021
- Ghaderi, G. M., Sadeghi, M. A., Alami, M., Azizi, M. H. and Ghorbani, M. (2012). Study on Antioxidant Activities of Phenolic Extracts from Fruit of a Variety of Iranian Acorn ( castaneifolia var castaneifolia). Iranian journal of food science and technology, 35(9):45-56.
- Gharekhani, M., Ghorbani, M., Ebrahimzadeh, M., Jaafari, S. and Sadeghi Mahoonak, A. (2010). 'Compare different methods of phenolic and flavonoid compounds extraction from Urtica dioica L.', Iranian Journal of Medicinal and Aromatic Plants Research, 26(3):389-405.
- Gohari, A., Hakim Zadeh V. and Golmakani, E. (2018). Optimizing of Temperature, Time and Solvent Concentration in Extraction of Antioxidant Extract from Walnut Interstitial Tissue During Ultrasound Process. Journal of food science and technology(Iran), 15(77):154-145
- Goli, A. H., Barzegar, M. and Sahari, M. A. 2005. Antioxidant activity and total phenolic compounds of pistachio (Pistachia Vera) hull extracts. Food Chemistry, 92(3):521-525. https://doi.org/10.1016/j.foodchem.2004.08.020
- Han, H., Wang, S., Rakita, M., Wang, Y., Han, Q. and Xu, Q. 2018. Effect of ultrasound-assisted extraction of phenolic compounds on the characteristics of walnut shells. Food and Nutrition Sciences, 9(8):1034-1045. DOI: 4236/fns.2018.98076
- Jiménez, A., Beltrán, G., & Uceda, M. (2007). High-power ultrasound in olive paste pretreatment. Effect on process yield and virgin olive oil characteristics. Ultrasonics sonochemistry, 14(6):725-731.
- Kamkar, A., Shariatifar, N., Jamshidi, A. H. Mohamadian, M. (2010). Evaluate the performance of the antioxidant water, methanol and ethanol extract cumin and Blghst in vitro. Journal of Gonabad University of Medical Sciences and Health Services, 16(2): 37-45.
- Kaur, S. and Mondal, P. (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiol Experiment, 1(1): 00005.
- Kulačanin, D., Bijelić, S., Šućur, J., Bogdanović, B., Ercisli, S. and Malenčić, Đ. (2020). Antioxidant Activity and Polyphenolic Profile of Walnut (Juglans regia) Green Husks and Liqueurs. Serbian Journal of Agricultural Sciences, 69(1-2):5-11.
- Mirzaee, Z. and Dehghan, G. (2016). The relationship between the antioxidant activity with different amounts of phenolic compounds. Studies in Medical Sciences, 27(4):321-329.
- Mohagheghi, S. A., Poorazarang, H., Elhamirad, A. H., Dezashibi, Z. and Hematyar, N. (2010). Extraction of phenolic compounds from potato peel (Ramus variety) with solvent and ultrasound-assisted methods and evaluation of its antioxidant activity in soybean oil. Iranian journal of food science and technology, 8(1):81-91.
- Mortazaie, S., Rafiyian, M., Ansari-samani, R. and Shahinfard, N. (2013). Compare the concentration of phenolic compounds and antioxidant activity of eight clinical plants. Journal of Rafsanjan University of Medical Sciences, 12(7):519-530.
- Nasirifar, Z., Sadeghi, M. A. and Kamali, F. 2013. Effect of extraction condition with two ultrasonic methods on phenolic, flavonoids and DPPH free radical scavenging of Celtis australis extract. Electronic journal of food processing and preservation, 5(2):115-129.
- Noshirvani, N., Fasihi, H. and Moradipayam, A. (2015). Study on the antioxidant effects of extract and powder of green walnut hulls on the oxidation of sunflower oil. Iranian Journal of Nutrition Sciences & Food Technology, 10(3): 79-90.
- Oliveira, I., Sousa, A., Ferreira, I. C., Bento, A., Estevinho, L. and Pereira, J. A. (2008). Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia) green husks. Food and chemical toxicology, 46(7):2326-2331. https://doi.org/10.1016/j.fct.2008.03.017
- Paniwnyk, L., Beaufoy, E., Lorimer, J. P. and Mason, J. (2001). The extraction of rutin from flower buds of Sophora japonica. Ultrasonics Sonochemistry, 8(3): 299–301. https://doi.org/10.1016/S1350-4177(00)00075-4
- Rahimipanah, M., Hamedi, M. and Mirzapour, M. (2011). Analysis of some factors affecting the phenolic compounds extracted from green husk of walnut (Juglans regia). Iranian Journal of Medicinal and Aromatic Plants Research, 27(3): 419-430. DOI: 10.22092/IJMAPR.2011.6383
- Rezai Erami, S., Jafari, S., Khamiri S. M. Bayat, H. (2015). Isolation of Shahmirzadi Husk Walnut Extract Using Microwave Assisted Extraction (MAE) and Evaluation of its Antioxidant Activity. Journal of Food Technology and Nutrition, 12(3):85-98.
- Rezai, E. S., Jafari, S. M., Khomeiri, M. Bayat, H. (2012). Antioxidant activity of toyserkani variety of walnut husk and comparison of its antiradical activity with synthetic antioxidants. journal of food research (university of tabriz), 22(1): 39-50.
- Rodriguez-Rojo, S., Visentin, A., Maestri, D. Cocero, M. J. (2012). Assisted extraction of rosemary antioxidants with green solvents. Journal of Food Engineering, 109(1):98–103. https://doi.org/10.1016/j.jfoodeng.2011.09.029
- Sanadgol, E., Raftani Amiri, Z. Mohammadzadeh Millani, J. (2018). Effect of different extraction methods on total phenolic content and antioxidant activity of the walnut (Juglans regia) leaves from Damavand, Shahrood and Hezarjarib. Innovative Food Technologies, 5(2), 349-359. DOI: 10.22104/JIFT.2017.2203.1510
- Shabanian, M., Sari, A. A. and Daraei Garmakhany, A. (2021).Optimization of ethanolic extracts of walnut green peel extracted by Microwave method and investigation of their antioxidant properties. Journal of Food Science & Technology (Iran), 18(115):283-298.
- Sun, T., powers M. R. Tang, J. (2007). Evalurion of the antioxidant activity of asparagus, broccoli and their juices. Food Chemistry, 105(1): 101-106. https://doi.org/10.1016/j.foodchem.2007.03.048
- Thompson, L. H. Doraiswamy, L. K. (1999). Sonochemistry: science and engineering. Industrial & Engineering Chemistry Research, 38(4): 1215-1249. https://doi.org/10.1021/ie9804172
- Vahdat Shariatpanahi, Z. Shahbaazi, S. (2013). Effects of Inner Part of Walnut on Serum Triglyceride and HDL Cholesterol Level in Hypertriglyceridemic Subjects. Journal of Islamic and Iranian Traditional Medicine, 4(2):97-103.
- Wang, S., Fu, W., Han, H., Rakita, M., Han, Q. Xu, Q. (2020). Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Walnut Shells and Characterization of Their Antioxidant Activities. Journal of Food and Nutrition Research, 8(1): 50-57.
- Yang, J., Chen, C., Zhao, S., Ge, F., Liu, D. (2014). Effect of solvents on the antioxidant activity of walnut (Juglans regia) shell extracts. Journal of Food and Nutrition Research, 2 (9): 621-626.
- Zhang, Z., Liao, L., Moore, J., Wu, T. & Wang, Z. (2009). Antioxidant phenolic compounds from walnut kernels (Juglans regia). Food Chemistry, 113(1): 160-165.
- Ziaolhagh, H. (2012). Study on the best packaging material and packaging conditions on the rancidity of almond kernels. Horticulturae, 963:113-118. DOI:10.17660/ActaHortic.2012.963.20.
- Ziaolhagh, S., Fatemian, H. Goodarzi, F. (2020). The Effect of Thermal Pretreatment and Packaging Conditions on the Shelf-life of Walnut Kernels. Journal of Nuts, 11(2):159-168.
Send comment about this article