with the collaboration of Iranian Food Science and Technology Association (IFSTA)

Document Type : Full Research Paper

Authors

Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

[1]Introduction: Today, the incidence of non-communicable and emerging diseases is increasing due to lifestyle changes, reduced mobility and changing dietary patterns. Some clinical evidences in simulated samples and real cases show that some compounds and plant extracts have a significant effect on the prevention and even treatment of these diseases. On the other hand, due to the structural and functional diversity of plant polysaccharides, there is a great tendency among researchers to find new polysaccharides in different sources with new functional and bioactive properties. Despite extensive studies in this field, no study has been done on the extraction of polysaccharide compounds with prebiotic properties from green almond hull as one of the agricultural wastes. Therefore, the purpose of this study was to introduce a new type of synbiotic compound to balance clone microbiota and promote consumer health.
 
Materials and methods: In this study, after extraction of water-soluble almond hull polysaccharides (AHP) by hot water extraction and precipitation with alcohol, the chemical analysis was done. To investigate the chemical composition of AHP, phenol sulfuric acid test was used to measure total sugar and Bradford test was used to measure protein. The amount of fat and ash in the sample was measured using standard methods (AOAC, 2005) and (AOAC, 2000), respectively. The amount of uronic acid of AHP was measured by calorimetry using metahydroxyphenyl at a wavelength of 520 nm. The content of AHP phenolic compounds was investigated by Folin Siocalcu calorimetric method. Fourier transform infrared (FT-IR) was also used to identify the functional groups and the anomeric status of AHP components. The prebiotic effect of this compound was also tested by digestion resistance and also by growth stimulation of the probiotic strain of Lactobacillus casei ATCC 393 in vitro for the first time.
 
Results and discussion: Chemical analysis showed that AHP is a heteropolysaccharide consisting of 86.30% w/w of total sugar, 5.10% w/w protein and 3.21% w/w uronic acid. FT-IR analysis also confirmed the chemical structure of AHP as a heteropolysaccharide. The results of digestion resistance showed that 91.24% of AHP can remain stable and undecomposed after the stages of gastrointestinal digestion, while this rate was 74.94% for inulin as a commercial prebiotic. The second prebiotic property of AHP investigated in this study was the stimulation the growth of Lactobacillus casei ATCC 393 as probiotic in sugar-free MRS-based culture media and the results showed that AHP compared to inulin significantly increased the survival of Lactobacillus casei ATCC 393 (p <0.01). The proliferation index in media containing AHP and inulin showed a significant difference and AHP stimulated the growth of Lactobacillus casei ATCC
393 significantly more than inulin (p <0.01). Therefore, to design a synbiotic product, if AHP is used as a prebiotic, the probiotic strain of Lactobacillus casei ATCC 393 would be a good choice.
Considering the annual production of thousands of tons of almond green hull waste in Iran and the concerns related to environmental problems caused by its accumulation, the introduction of industrially feasible and economically justified methods to produce value-added products from this agricultural waste seems essential. In the present study, polysaccharides extracted from almond green hull by hot water extraction and alcohol precipitation, which is an economically feasible method and can be implemented on an industrial scale, were introduced as a useful compound. In vitro studies also used culture medium containing AHP as a commercial prebiotic in comparison with culture medium containing inulin. The results showed that this compound has a good resistance to digestive conditions in the gastrointestinal tract compared to inulin. The compound was also able to stimulate the growth of the probiotic Lactobacillus casei ATCC 393 in culture medium. In general, in this study, a new synbiotic compound including Lactobacillus casei ATCC 393 and AHP was introduced as a health beneficial food additive.

Keywords

Main Subjects

  1. AOAC (Association of Official Analytical Chemists). (2005). Method of analysis Gaithersburg. AOAC International, Gaithersburg, MD, 1–38.
  2. Biedrzycka, E., & Bielecka, M. (2004). Prebiotic effectiveness of fructans of different degrees of polymerization. Trends in Food Science and Technology, 15(3–4), 170–175. https://doi.org/10.1016/j.tifs.2003.09.014. https://doi.org/10.1016/j.tifs.2003.09.014
  3. Blumenkrantz, N., & Asboe-Hansen, G. (1973). New method for quantitative determination of uronic acids. Analytical Biochemistry, 54(2), 484–489. https://doi.org/10.1016/0003-2697(73)90377-1
  4. Carvalho, A. F. U., Portela, M. C. C., Sousa, M. B., Martins, F. S., Rocha, F. C., Farias, D. F., & Feitosa, J. P. A. (2009). Physiological and physico-chemical characterization of dietary fibre from the green seaweed Ulva fasciata Delile. Brazilian Journal of Biology, 69(3), 969–977.
  5. Dawood, D. H., Darwish, M. S., El-Awady, A. A., Mohamed, A. H., Zaki, A. A., & Taher, M. A. (2021). Chemical characterization of Cassia fistula polysaccharide (CFP) and its potential application as a prebiotic in synbiotic preparation. RSC Advances, 11(22), 13329–13340.
  6. do Nascimento Santos, D. K. D., da Silva Barros, B. R., da Cruz Filho, I. J., Júnior, N. da S. B., da Silva, P. R., do Bomfim Nascimento, P. H., de Lima, M. do C. A., Napoleão, T. H., & de Melo, C. M. L. (2021). Pectin-like polysaccharide extracted from the leaves of Conocarpus erectus Linnaeus promotes antioxidant, immunomodulatory and prebiotic effects. Bioactive Carbohydrates and Dietary Fibre, 26, 100263. https://doi.org/10.1016/j.bcdf.2021.100263
  7. Elahi, M. Y., Kargar, H., Dindarlou, M. S., Kholif, A. E., Elghandour, M. M. Y., Rojas-Hernández, S., Odongo, N. E., & Salem, A. Z. M. (2017). The chemical composition and in vitro digestibility evaluation of almond tree (Prunus dulcis DA Webb syn. Prunus amygdalus; var. Shokoufeh) leaves versus hulls and green versus dry leaves as feed for ruminants. Agroforestry Systems, 91(4), 773–780. https://doi.org/10.1007/s10457-016-9964-5
  8. Firdaus, A., Nurul, M., Mustafa, S., Hashim, D., & Manap, Y. A. (2012). Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots. Molecules, 17, 1635–1651. https://doi.org/10.3390/molecules17021635
  9. Gllibowski, P., & Bukowska, A. (2011). The effect of pH, temperature and heating time on inulin chemical stabilityH. Acta Scientiarum Polonorum Technologia Alimentaria, 10(2), 189–196. https://doi.org/10.1007/s00217-004-1098-8
  10. Grootaert, C., Delcour, J. A., Courtin, C. M., Broekaert, W. F., Verstraete, W., & Van de Wiele, T. (2007). Microbial metabolism and prebiotic potency of arabinoxylan oligosaccharides in the human intestine. Trends in Food Science & Technology, 18(2), 64–71. https://doi.org/10.1016/j.tifs.2006.08.004
  11. Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D. J., Pot, B., Morelli, L., Canani, R. B., Flint, H. J., Salminen, S., Calder, P. C., & Sanders, M. E. (2014). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology, 11(8), 506–514. https://doi.org/10.1038/nrgastro.2014.66
  12. Huang, F., Liu, H., Zhang, R., Dong, L., Liu, L., Ma, Y., Jia, X., Wang, G., & Zhang, M. (2019). Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques. Carbohydrate Polymers, 206, 344–351. https://doi.org/10.1016/j.carbpol.2018.11.012
  13. Huebner, J., Wehling, R. L., Parkhurst, A., & Hutkins, R. W. (2008). Effect of processing conditions on the prebiotic activity of commercial prebiotics. International Dairy Journal, 18(3), 287–293. https://doi.org/10.1016/j.idairyj.2007.08.013
  14. Jain, S. K., Jain, A., Gupta, Y., & Ahirwar, M. (2007). Design and development of hydrogel beads for targeted drug delivery to the colon. AAPS PharmSciTech, 8(3), 34–41. https://doi.org/10.1208/pt0803056
  15. Jayamanohar, J., Devi, P. B., Kavitake, D., Priyadarisini, V. B., & Shetty, P. H. (2019). Prebiotic potential of water extractable polysaccharide from red kidney bean (Phaseolus vulgaris). Lwt, 101, 703–710. https://doi.org/10.1016/j.lwt.2018.11.089
  16. Luo, A., He, X., Zhou, S., Fan, Y., He, T., & Chun, Z. (2009). In vitro antioxidant activities of a water-soluble polysaccharide derived from Dendrobium nobile Lindl. extracts. International Journal of Biological Macromolecules, 45(4), 359–363. https://doi.org/10.1016/j.ijbiomac.2009.07.008
  17. Miles, A. A., Misra, S. S., & Irwin, J. O. (1938). The estimation of the bactericidal power of the blood. The Journal of Hygiene, 38(6), 732–749. https://doi.org/10.1017/S002217240001158X
  18. Nep, E. I., Sims, I. M., Morris, G. A., & Kontogiorgos, V. (2015). Evaluation of some important physicochemical properties of starch free grewia gum. Food Hydrocolloids, 15, 77–76. https://doi.org/10.1016/j.foodhyd.2015.02.016.
  19. Slavov, A., Panchev, I., Kovacheva, D., & Vasileva, I. (2016). Physico-chemical characterization of water-soluble pectic extracts from Rosa damascena, Calendula officinalis and Matricaria chamomilla wastes. Food Hydrocolloids, 61, 469–476. https://doi.org/10.1016/j.foodhyd.2016.06.006
  20. Tadayoni, M., Sheikh-Zeinoddin, M., & Soleimanian-Zad, S. (2015). Isolation of bioactive polysaccharide from acorn and evaluation of its functional properties. International Journal of Biological Macromolecules, 72, 179–184. https://doi.org/10.1016/j.ijbiomac.2014.08.015
  21. Taylor, P., Woisky, R. G., & Salatino, A. (2015). control Analysis of propolis : some parameters and procedures for chemical quality control. Journal of Apicultural Research, April, 37–41. https://doi.org/10.1080/00218839.1998.11100961
  22. Wang, X., Huang, M., Yang, F., Sun, H., Zhou, X., Guo, Y., Wang, X., & Zhang, M. (2015). Rapeseed polysaccharides as prebiotics on growth and acidifying activity of probiotics in vitro. Carbohydrate Polymers, 125, 232–240. https://doi.org/10.1016/j.carbpol.2015.02.040
  23. Wichienchot, S., Jatupornpipat, M., & Rastall, R. a. (2010). Oligosaccharides of pitaya (dragon fruit) flesh and their prebiotic properties. Food Chemistry, 120(3), 850–857. https://doi.org/10.1016/j.foodchem.2009.11.026
  24. Wichienchot, S., Thammarutwasik, P., Jongjareonrak, A., Chansuwan, W., Hmadhlu, P., Hongpattarakere, T., Itharat, A., & Ooraikul, B. (2011). Extraction and analysis of prebiotics from selected plants from southern Thailand. Sonklanakarin Journal of Science and Technology, 33(5), 517.
  25. Xie, J.-H., Liu, X., Shen, M. Y., Nie, S. P., Zhang, H., Li, C., Gong, D. M., & Xie, M. Y. (2013). Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves. Food Chemistry, 136(3), 1453–1460. https://doi.org/10.1016/j.foodchem.2012.09.078
  26. Zhu, J., Liu, W., Yu, J., Zou, S., Wang, J., Yao, W., & Gao, X. (2013). Characterization and hypoglycemic effect of a polysaccharide extracted from the fruit of Lycium barbarum L. Carbohydrate Polymers, 98(1), 8–16. https://doi.org/10.1016/j.carbpol.2013.04.057
  27. Zhu, W., Zhou, S., Liu, J., McLean, R. J. C., & Chu, W. (2020). Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide. Biomedicine & Pharmacotherapy, 121, 109591. https://doi.org/10.1016/j.biopha.2019.109591
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