Effect of Ultrasonic Waves on Structural, Functional and Rheological Properties of Locust Bean Gum

Document Type : Research Article

Authors

• Samira Farizad
• Hajar Abbasi

Department of Food Science and Technology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

https://doi.org/10.22067/ifstrj.2022.74595.1133

Abstract

Introduction
 Hydrocolloid is a term refers to all polysaccharides extracted from plants, seeds, and microbial sources that regardless of their biological and nutritional role has various functional properties in food products such as concentration and gel production of aqueous solutions, stabilization of foam, emulsions and dispersed systems, prevention of ice and sugar crystals, control of the release of flavor compounds and consequently control and improve food quality. Ultrasound has been widely used in the food industry due to its numerous physical and chemical effects. The effect of ultrasound is due to cavitation, heating, dynamic mobility and shear stresses to the sample. Today, ultrasound is used as a green new technology with unique effects on food storage and processing. One of the newest applications of ultrasound is altering the structure of polymers such as polysaccharides. Changes in the structure of hydrocolloids lead to modification in their functional properties.
 
Materials and Methods
 In this study, effect of ultrasonic waves, time (0-90 min) and processing temperature (25-75 °C) on physicochemical, rheological and functional properties of locust bean gum was investigated. In this regards, different parameters including changes in pH, solubility, minimum gelatinization concentration, viscosity and emulsifying properties (Emulsifying capacity, emulsion stability and particle dimensions) of treated locust bean gum were determined. Response surface methodology in central composite design was used to evaluate the effect of independent variables on qualitative properties of locust bean gum and model their changes. The best treatment condition was determined and the optimum treated samples were evaluated and compared in microstructure using scanning electron microscopy, rheological properties (rotational and oscillatory test) and Fourier transform infra-red.
 
Results and Discussion
 The results showed that with increasing the time of ultrasound treatment, pH, and viscosity of the hydrocolloid decreased and its solubility and minimum gel concentration increased. However, with increasing temperature, pH, minimum gelatinization concentration and hydrocolloid viscosity increased and its solubility decreased. Optimization of treatment conditions was performed by considering the achievement of the best hydrocolloid performance characteristics. Two different temperature and time conditions were proposed for the desirable treatment of the hydrocolloid using ultrasound. In the first case, the selection criteria were to achieve the best solubility and emulsifying properties of the hydrocolloid, and in the second case, the selection criteria were solubility and emulsifying properties improvement while maintaining the gelatinization properties of the hydrocolloid. Based on the desired quality factors, optimization was performed and the results showed that in the first optimal sample (treated at 40 ° C and 48.9 minutes), solubility and emulsifying properties and in the second optimal sample (treated at 66.67 ° C and 15 Min) by maintaining the gelation properties of hydrocolloid, the solubility of the hydrocolloid increased significantly. The performance of treated samples validated developed models. SEM results showed that the ultrasound increased three-dimensional structure of gum. The smaller microscopic structure was observed in untreated sample and the larger one was in treated with ultrasonic waves for 40.9 minutes at 40 ° C. Therefore, ultrasound caused agglomeration of treated freeze dried gum. It is noteworthy that several holes observed in the structure of treated gum with ultrasonic, which can increase solubility of the gum. The flow behavior of the sonicated and control samples showed that the viscosity of all samples decreased with increasing shear rate, which indicates their pseudoplastic behavior. At low shear rates (about 0.01 per second), the viscosity of the control sample was higher than that of the sonicated samples. However, at high shear rates (about 40 per second), viscosity of the three samples were almost the same. Therefore, the control sample was more affective to shear rate compared to the treated samples. The modulus of elasticity (G ') and viscosity (G' ') of the treated specimens were lower compared to the control. Also, the frequency sweep of samples shown that in the frequency range under study, G 'and G'' were frequency dependent and with increasing frequency, the amount of these two parameters increased. This observation indicates the influence of rheological properties of the samples on the frequency changes that are commonly observed in suspensions. At low frequencies, the predominance of viscous behavior over elastic was observed in all three control and ultrasonic samples (G ''> G ') and this feature was higher in the treated samples than the control. The modulus of elasticity and viscosity of the treated sample for 49 minutes was lower than other treated samples for 15 minutes, which shows the effect of ultrasound on the rheological properties of the hydrocolloid.
 
Conclusion
 The results of this study showed that ultrasonic waves provide a good opportunity to change the physical and functional properties of carob seed gum. It is possible to significantly improve the solubility and emulsifying activity of this hydrocolloid using ultrasound. It is necessary to determine the treatment conditions of the sample according to the intended application.

Keywords

• Locust bean gum
• Rheology
• Solubility
• Ultrasound
• Viscosity

Main Subjects

• Food Technology

References

1.  

   1. Barak, S., & Mudgil, (2014). Locust bean gum: processing, properties and food applications-a review. International Journal of Biological Macromolecules, 66, 74-80. https://doi.org/10.1016/j.ijbiomac.2014.02.017
   2. Camino, N.A., Pérez, O.E., & Pilosof, A. (2009). Molecular and functional modification of hydroxypropylmethylcellulose by high-intensity ultrasound. Food Hydrocolloids, 23, 1089-1095. https://doi.org/10.1016/j.foodhyd.2008.08.015
   3. Chen, R.H., Chang, J.R., & Shyur, J.S. (1997). Effects of ultrasound conditions and storage in acidic solutions on changes in molecular weight and polydispersity of treated chitosan. Carbohydrate Research, 299, 287–294. https://doi.org/10.1016/S0008-6215(97)00019-0
   4. Dickinson, (2003). Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocolloids, 17, 25-39. https://doi.org/10.1016/S0268-005X(01)00120-5
   5. Dickinson, (2009). Hydrocolloids as emulsifiers and emulsion stabilizers. Food Hydrocolloids, 23, 1473-1482. https://doi.org/10.1016/j.foodhyd.2008.08.005
   6. Darab Zadeh,, & Farahnaki, A. (2010). The comparison of physicochemical and rheological properties between Iranian and commercial carob bean gum. In proceedings of 19^th National Congress on Food Science and Technology, 12– 13 Nov., Tehran University, Tehran-Iran.
   7. Farzi, M., Saffari, M.M., Emam‐Djomeh, Z., & Mohammadifar, M. (2011). Effect of ultrasonic treatment on the rheological properties and particle size of gum tragacanth dispersions from different species. International Journal of Food Science & Technology, 46, 849-854. https://doi.org/10.1111/j.1365-2621.2011.02558.x
   8. Feng, L., Cao, Y., Xu, D., Wang, S., & Zhang, (2017). Molecular weight distribution, rheological property and structural changes of sodium alginate induced by ultrasound. Ultrasonics Sonochemistry, 34, 609-615. https://doi.org/10.1016/j.ultsonch.2016.06.038
   9. Huang, X., Kakuda, Y., & Cui, (2001). Hydrocolloids in emulsions: particle size distribution and interfacial activity. Food Hydrocolloids, 15, 533-542. https://doi.org/10.1016/S0268-005X(01)00091-1
   10. Hosseini, S.M.H., Emam-Djomeh, Z., Razavi, S.H., & Moosavi-Movahedi, A. (2013). Beta-Lactoglobuline-sodium alginate interaction as affected by polysaccharide depolymerization using high intensity ultrasound. Food Hydrocolloids, 32, 235–244. https://doi.org/10.1016/j.foodhyd.2013.01.002
   11. Koda, S., Taguchi, K., & Futamura, (2011). Effects of frequency and a radical scavenger on ultrasonic degradation of watersoluble polymers. Ultrasound Sonochemistry, 18, 276–281. https://doi.org/10.1016/j.ultsonch.2010.06.007
   12. Li, J., Li, B., Geng, P., Song, A.X., & Wu, J. (2017). Ultrasonic degradation kinetics and rheological profiles of a food polysaccharide (konjac glucomannan) in water. Food Hydrocolloids, 70, 14-19. https://doi.org/10.1016/j.foodhyd.2017.03.022
   13. Iida, Y., Tuziuti, T., Yasui, K., Towata, A., & Kozuka, T. (2008). Control of viscosity in starch and polysaccharide solutions with ultrasound after gelatinization. Innovative Food Science & Emerging Technologies, 9, 140–146. https://doi.org/10.1016/j.ifset.2007.03.029
   14. Mason, T.J., Paniwnyk, L., & Lorimer,P. (1996). The uses of ultrasound in food technology. Ultrasonics Sonochemistry, 3, 253-260. https://doi.org/10.1016/S1350-4177(96)00034-X
   15. Mishra, S., Mann, B., & Joshi,K. (2001). Functional improvement of whey protein concentrate on interaction with pectin. Food Hydrocolloids, 15, 9-15. https://doi.org/10.1016/S0268-005X(00)00043-6
   16. Peres, G.L., Leite, D.C., & Silveira, N.P. (2015). Ultrasound effect on molecular weight reduction of amylopectin. Starch‐Stärke, 67, 407-414. https://doi.org/10.1002/star.201400230
   17. Prajapat, A.L., & Gogate, P. (2015). Depolymerization of guar gum solution using different approaches based on ultrasound and microwave irradiations. Chemical Engineering and Processing: Process Intensification, 88, 1-9. https://doi.org/10.1016/j.cep.2014.11.018
   18. Razavi, S.M.A., Bostan, A., Niknia, S., & Razmkhah, S. (2011). Functional properties of hydrocolloid extracted from selected domestic Iranian seeds. Journal of Food Research, 21(3), 379-389.
   19. Samari Khalaj, M., & Abbasi, S. (2014). Influence of chemical modification on solubility of insoluble fraction of Persian gum. Research and Innovation in Food Science and Technology, 3(2), 171-184. https://doi.org/10.22101/JRIFST.2014.08.23.326
   20. Sujka, M., & Jamroz, J. (2013). Ultrasound-treated starch: SEM and TEM imaging, and functional behaviour. Food Hydrocolloid, 31, 413–419. https://doi.org/10.1016/j.foodhyd.2012.11.027
   21. Tiwari, B.K., Mthkmarappan, K., O’Donnell, C.P., & Cllen, P. (2010). Rheological Properties of Sonicated Guar, Xanthan and Pectin Dispersions. International Journal of Food Properties, 13, 223-233. https://doi.org/10.1080/10942910802317610
   22. Wang, Z.M., Cheung, Y.C., Leung, P.H., & Wu, J. (2010). Ultrasonic treatment for improved solution properties of a high-molecular weight exopolysaccharide produced by a medicinal fungus. Bioresource Technology, 101, 5517-5522. https://doi.org/10.1016/j.biortech.2010.01.134
   23. Wang, Z., Han, F., Sui, X., Qi, B., Yang, Y., Zhang, H., & Jiang, L. (2016). Effect of ultrasound treatment on the wet heating Maillard reaction between mung bean [Vigna radiate (L.)] protein isolates and glucose and on structural and physico‐chemical properties of conjugates. Journal of the Science of Food and Agriculture, 96(5), 1532-1540. https://doi.org/10.1002/jsfa.7255
   24. Zhang, L., Xingqian, Y., Sophia, J.X., Xianzhong, Z., Donghong, L., Ruifeng, M., & Shiguo, C. (2013). Effect of high-intensity ultrasound on the physicochemical properties and nanostructure of citrus pectin. Journal of the Science of Food and Agriculture, 93(8), 2028-20 https://doi.org/10.1002/jsfa.6011