Evaluation of Antioxidant Effect of Ethanolic Extract of Aloe Vera Gel on the Stability of Soybean Oil

Document Type : Research Article

Authors

• Marjan Karami
• Leila Nateghi
• Simin Asadollahi

Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

https://doi.org/10.22067/ifstrj.2022.77602.1189

Abstract

Introduction
 Oxidation of lipids results in changes that may affect the nutritional quality, wholesomeness, colour, flavour and texture of food. The aim of this study was to investigate the type and amount of phenolic compounds in ethanolic extract of aloe vera gel as a source of natural antioxidant and its effect on the oxidative stability of soybean oil.
Using synthetic antioxidant due to the possibility of toxic and carcinogenic effects is limited. Thus, it is important to find an alternative to synthetic antioxidants by natural antioxidant. Different intrinsic and extrinsic factors may initiate the oxidation of lipids. The initial products of oxidation are tasteless and odorless and after degradation and production of secondary products, the off-flavors and off-odors will appeared in edible oils. This is a great concern in food industry, because it decreases the shelf life of food products. Free radicals are produced during chain reactions in lipid oxidation process. To avoid this, synthetic antioxidants are usually used which are sensible to heat and are hazardous to human health and may cause cancer. Polyphenols have antioxidant activity and absorb free radicals. Thus, the vegetable oils rich in polyphenols can affect human health. In this research, we aimed to investigate the application of natural extract of aloe vera gel as a natural antioxidant to avoid soy oil oxidation and to compare it with synthetic antioxidants.
The rate of oxidation reaction can be delayed by adding antioxidants. Consumers today tend to use natural antioxidants instead of synthetics. The overall purpose of this study was to investigate the effect of adding ethanolic extract of aloe vera gel as natural antioxidant on improving the stability of soybean oil.
 
Materials and Methods
 The compounds in ethanolic extract of aloe vera gel were determined using GC / MS. The antioxidant activity was evaluated by the method of DPPH. For this purpose, ethanolic extracts of aloe vera gel were added to soybean oil in four different concentrations (500, 1000, 1500, 2000 ppm) and peroxide value, acidity, thiobarbituric acid, total phenol, oxidative stability to Rancimat method, fatty acid profile and sensory evaluation were performed on soybean oil samples and compared with the control sample containing 120 ppm BHA and soybean oil sample without adding antioxidants during 30, 60 and 90 days of storage at 25 ° C.
 
Results and Discussion
 The results showed that by increasing the concentration of aloe vera extracts from 500 to 2000 ppm, the oxidation rate decreases during 90 days of storage, the amount of peroxide, thiobarbituric acid and acidity of soybean oil containing 2000 ppm ethanolic extract of aloe vera gel was lower than the control sample containing 120 ppm BHA. The total phenol content and free radical scavenging and stability to oxidative degradation by Rancimat method in soybean oil sample containing 2000 ppm aloe vera ethanol extract was higher than soybean oil samples containing BHA120 ppm. Evaluation of sensory properties showed that no significant difference was observed between the sensory properties of the oil sample containing 2000 ppm ethanolic extract of aloe vera gel and the control soybean oil sample of BHA120 ppm.
 
Conclusion
 Considering that the sample of soybean oil containing 2000 ppm ethanolic extract of aloe vera gel had higher phenol content and free radical scavenging and more antioxidant properties than the control sample, it did not differ significantly from the control sample. Qualitative and health properties were selected as the superior treatment. The results of this study showed that the ethanolic extract of aloe vera gel can be used as a natural antioxidant instead of conventional synthetic antioxidants in the oil industry and to prevent oxidative spoilage of the oil in a desirable way. Therefore, it might be employed as a natural antioxidant in foods, particularly those containing edible oils.

Keywords

• Aloe vera gel
• Antioxidant activity Soybean oil

Main Subjects

• Food Technology

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

References

1. Ahmadi, F., Kadivar, M., & Shahedi, M. (2007). Antioxidant activity of kelussia odoratissima moza, in model and food systems. Food Chemistry, 105, 57-64. https://doi.org/10.1016/j.foodchem.2007.03.056
2. Ayoubi, A., Omidi, A., Valizade, R., & Mousaei, A. (2013). Effect of hydroalcoholic extract of Aloe vera and Teucrium on serum glucose and lipid profile in streptozotocin diabetic male rats. Journal Birjand Univercitu Medical Science, 20(2), 144-152. (In Persian). http://journal.bums.ac.ir/article-1-1335-fa.html
3. Bazari Moghaddam, S., Haghighi, M., Sharif Rohani, M., Hamidi, M., & Ghasemi, M. (2016). Effects of Aloe vera extract on growth indices, carcass composition and bacterial flora of intestine in Siberian sturgeon (Acipenser baerii). Iranian Scientific Fisheries Journal, 25(1), 40-53. (In Persian). https://doi.org/10.22092/ISFJ.2017.110222
4. Bhargawa, S., Kapoor, S., Ranote, P.S., & Sharma, S. (2014). Studies on aloe juice supplemented kinnow nectar. Research Jouranal Agriculture and Forestry Science, 2(8), 14-20. http://www.isca.me/AGRI_FORESTRY/Archive/v2/i8/3.ISCA-RJAFS-2014-041.pdf
5. Boghani, A.H., Raheem, A., & Hashmi, S.I. (2012). Development and storage studies of blended papaya-Aloe vera ready to serve (RTS) beverage. Jornal of Food Process Technology, 3(10), 2. https://doi.org/10.4172/2157-7110.1000185
6. Bubonja-Sonje, M., Giacometti, J., & Abram, M. (2011). Antioxidant and antilisterial activity of olive oil, cocoa and rosemary extract polyphenols. Food Chemistry, 127(4), 1821-7. https://doi.org/10.1016/j.foodchem.2011.02.071
7. Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10, 178-182. https://doi.org/10.38212/2224-6614.2748
8. Chang, X.L., Chen, B.Y., & Feng, Y.M. (2011). Water-soluble polysaccharides isolated from skin juice, gel juice and flower of Aloe vera Miller. Journal of the Taiwan Institute of Chemical Engineers, 42(2), 197-203. https://doi.org/10.1016/j.jtice.2010.07.007
9. Dordevic, S., & Petrovic, S. (2007). Antimicrobial, anti-inflammatory, anti-ulcer and antioxidant activities of Carlina acanthifolia root essential oil. Journal of Ethnopharmacol, 109, 458–63. https://doi.org/10.1016/j.jep.2006.08.021
10. Dehghan, B., Esmaeilzadeh Kenari, R., & Raftani Amiri, Z. (2019). Investigate the antioxidant properties of orange peel essential oil (Citrus sinesis) on the stability of soybean oil during storage conditions. Food Technology & Nutrition, 16(3), 73-90. (In Persian). https://jftn.srbiau.ac.ir/article_14371.html?lang=en
11. Ebrahimzadeh, M.A., Pourmorad, F., & Hafezi, S. (2008). Antioxidant activities of Iranian corn silk. Turkish Journal of Biology, 32, 43-49. https://journals.tubitak.gov.tr/biology/vol32/iss1/7
12. El-Shemy, H.A., Aboul-Soud, M.A., Nassr- Allah, A.A., Aboul-Enein, K.M., Kabash, A., & Yagi, A. (2010). Antitumor properties and modulation of antioxidant enzymes activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction. Current Medicinal Chemistry, 17(2), 129-38. https://doi.org/10.2174/092986710790112620
13. Esfandiarifard, M., & Ziaolhagh, S. (2021). Study on the antioxidant activity of propolis extract and its effect on the oxidation of sunflower oil. Biochemistry and Forensic Science, 17(107), 119-130. https://doi.org/52547/fsct.17.107.119
14. Farhoosh, R. (2007). The effect of operational parameters of the Rancimat method on the determination of the oxidative stability measures and shelf-life prediction of soybean oil. Journal of American Oil Chemistry Society, 84, 205-209. https://link.springer.com/article/10.1007/s11746-006-1030-4
15. Froehlicher, T., Hennebelle, T., Martin-Nizard, F., Cleenewerck, P., Hilbert, J.L., Trotin, F., & Grec, S. (2009). Phenolic profiles and antioxidative effects of hawthorn cell suspensions, fresh fruits, and medicinal dried parts. Food Chemistry, 115, 897–903. https://doi.org/10.1016/j.foodchem.2009.01.004
16. Gupta, V.K., & Malhotra, S. (2012). Pharmacological attribute of Aloe vera: revalidation through experimental and clinical studies. An International Quarterly Journal of Research in Ayurveda, 33(2), 193–196. https://doi.org/10.4103/0974-8520.105237
17. Hinneburg, I., Damien Dorman, H.J., & Hiltunen, R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Journal of Food Chemistry, 97, 122–129. https://doi.org/10.1016/j.foodchem.2005.03.028
18. Iqbal, S., & Bhanger, M. (2007). Stabilization of sunflower oil by garlic extract during accelerated storage. Food Chemistry, 100, 246–254. https://doi.org/10.1016/j.foodchem.2005.09.049
19. Joseph, B. (2010). Pharmacognostic and phytochemical properties of Aloe vera linn – an overview. International Journal of Pharmaceutical Sciences Review and Research, 4(2), 34-42. https://www.scirp.org/(S(i43dyn45teexjx455qlt3d2q))/reference/ReferencesPapers.aspx?ReferenceID=996325
20. Kamkar, A., Tooryan, F., Akhondzadeh Basti, A., Misaghi, A., & Shariatifar, N. (2013). Chemical composition of summer savory (Satureja hortensis ) essential oil and comparison of antioxidant activity with aqueous and alcoholic extracts. Journal of Veterinary Reserch, 68, 2, 183-190. (In Persian). https://doi.org/10.22059/JVR.2013.31966
21. Kulisic, T., Radonic, A., Katalinic, V., & Milosa, M. (2004). Use of different methods for testing antioxidative activity of oregano essential oil, analytical, nutritional and clinical methods. Food Chemistry, 85, 633–640. https://doi.org/10.1016/j.foodchem.2003.07.024
22. Mazaheri Kalhori, M., Basiri, A., & Jalali, H. (2014). Evaluation of anti-oxidant properties of fennel seed essential oil (Foeniculum vulgare) and its effect on oxidative stability of soybean oil. Iranian Biosystem Engineering, 45(2), 131-139. (In Persian). https://doi.org/10.22104/JIFT.2014.41
23. Molyneux, P.H. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakar in Journal Science Technology, 26, 211-9. https://www.thaiscience.info/journals/article/song/10462423.pdf
24. Mohagheghi Samarin, A., Poorazarang, H., Elhamirad, A.H., Dezashibi1, Z., & Hematyar, N. (2011). Extraction of phenolic compounds from potato peel (Ramus variety) with solvent and ultrasound-assisted methods and evaluation of its antioxidant activity in soybean oil. Journal of Food Science and Technology, 8(1), 81-91. http://fsct.modares.ac.ir/article-7-5437-en.html
25. Mohammadi, A., Jafari, S.M., Esfanjani, A.F., & Akhavan, S. (2016). Application of nano-encapsulated olive leaf extract in controlling the oxidative stability of soybean oil. Food Chemistry, 190, 513-519. https://doi.org/10.1016/j.foodchem.2015.05.115
26. Mohammadi, A., & Arabshahi- Delouee, S. (2016). Antioxidant properties of extract Boswellia serrata oleo-gum resin in soyabean oil. Iranian Food Science and Technology Research Journal, 12(4), 477-488. (In Persian). https://doi.org/10.22067/ifstrj.v12i4.40783
27. Parizan, T., Elhamirad, A.H., Estiri, S.H., & Armin, M. (2011). Assessing antioxidant activity methanol extract of senna leaf and its effect on the stability of soybean oil. Inovations in Food Science and Technology, 1(7), 51-59. (In Persian). https://sid.ir/paper/176504/fa
28. Rafiei, Z., Jafari, S.M., Alami, M., & Khomeiri, M. (2011). Antioxidant properties of olive leaf extract and its application in sunflower oil. Journal of Food Research (University of Tabriz), 21(1), 11-23. (In Persian). https://foodresearch.tabrizu.ac.ir/article_938.html?lang=en
29. Rouzbehan, Y., Alipour, D., Barzegar, M., & Azizi, M.H. (2008). Antioxidant activity of phenolic compounds of grape pomace. International Journal of Food Science and Technology, 5(3), 69-74. https://sid.ir/paper/72424/en
30. Salehi, E.A., Esmailzadeh Kenari, R., & Nasiri Takami, T. (2014). Investigation of the effect of pomegranate extract (Pimppinella affinis Ledeb) on the stabilization of canola oil during storage conditions. Iranian Food Science and Technology Research Journal, 10(2), 176-181. (In Persian). https://ifstrj.um.ac.ir/article_33825_f546275fc322bf7f7ba17b9384341f13.pdf
31. Salta, F.N., Mylona, A., Chiou, A., Boskou, G., & Andrikopoulos, N.K. (2007). Oxidative stability of edible vegetable oils enriched in polyphenols with olive leaf extract. Food Science and Technology International, 13(6), 413-421. https://doi.org/10.1177/1082013208089563
32. Samad Louei, H.R., Azizi, M.H., & Barzegar, M. (2007). Antioxidative effect of pomegranate seed phenolic componends on soybean oil. Journal of Agricultural Sciences and Natural Resources, 14(4), 193-200. (In Persian). https://www.sid.ir/fa/VEWSSID/J_pdf/51413860416.pdf
33. Sasi Kumar, R., Ray, R.C., Paul, P.K., & Suresh, C.P. (2013). Development and storage studies of therapeutic ready to serve (RTS) made from blend of Aloe vera Aonla and ginger juice. Journal Food Process Technology, 4(232), 2. https://doi.org/10.4172/2157-7110.1000232
34. Selmi, S., Limam, Z., Batista, I., Bandarra, N.M., & Nunes, M. L. (2011). Effects of storage temperature and α tocopherol on oil recovered from sardine mince. International Journalof Refrigeration, 34(5), 1315-1322. https://doi.org/10.1016/j.ijrefrig.2010.08.018
35. Shahidi, F. (2005). Bailey’s industrial oil and fat products. (6^th edn), John Wiley & Sons, Inc, simultaneously in Canada. https://onlinelibrary.wiley.com/doi/book/10.1002/047167849X
36. Singh, G., Maurya, S., & Catalan, C. (2005). Chemical constituents antimicrobial investigations and antioxidant potentials of Anethum graveolens essential oil and acetone extract: part 52. Journal Food Science, 70, 208-215. https://doi.org/10.1111/j.1365-2621.2005.tb07190.x
37. Tahami, F.S., Basiri, A., Tarzi, B., & Mahasti, P. (2012). Evaluation of the antioxidant effect of fennel seed extract (Foeniculum vulgare) on the stability of sunflower oil. Food Technology & Nutrition, 10(1), 71-78. (In Persian). https://sid.ir/paper/143139/fa
38. Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: a review. International Pharmaceutica Sciencia, 1(1), 98-108. http://www.ipharmsciencia.com
39. Wang, W., Wu, N., Zu, Y.G., & Fu, Y.J. (2008). Antioxidative activity of Rosmarinus officinalis essential oil compared to its main components. Food Chemistry, 108, 1019–1022. https://doi.org/10.1016/j.foodchem.2007.11.046
40. Yaghmur, A., Aserin, A., Mizrahi, Y., Nerd, A., & Garti, N. (2001). Evaluation of argan oil for deep-fat frying. Lebensm Wiss. u.-Technology, 34, 124-130. https://doi.org/10.1006/fstl.2000.0697
41. Yao, X., Zhang, D., Zu, Y., Fu, Y., Luo, M., Gu, C., Li, C.M.F., & Efferth, T. (2013). Free radical scavenging capability, antioxidant activity and chemical constituents of Pyrola incarnata Leaves. Industrial Crops and Products, 49, 247–255. https://doi.org/10.1016/j.indcrop.2013.04.058