نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

دانشگاه صنعتی اصفهان

چکیده

هدف از این تحقیق بررسی کارایی الکتروریسی بدون نازل برای انکپسوله کردن اسانس زنیان (به‌عنوان یک زیست فعال آبگریز) با استفاده از دو هیدروکلوئید (کیتوزان/ژلاتین) به‌منظور افزایش خواص آنتی‌اکسیدانی و پایداری آن برای کاربردهای غذایی بود. نانوالیاف با استفاده از کیتوزان/ژلاتین در نسبت‌های 1:6، 1:8 و 1:10 و غلظت‌های 20 و 40 درصد زنیان ریسیده شدند. خواص محلول (ویسکوزیته و هدایت الکتریکی) اندازه‌گیری شد. داده‌های کارایی انکپسولاسیون و ظرفیت بارگذاری مبین بهبود با افزایش غلظت اسانس بود. قطر و مورفولوژی الیاف با میکروسکوپ الکترونی روبشی مورد بررسی قرار گرفت. نانوالیاف کیتوزان/ژلاتین با نسبت 1:6 حاوی 40 درصد اسانس دارای بیشترین کارایی انکپسولاسیون (9/99%)، ظرفیت بارگذاری (9/39%) و کمترین قطر ( nm146) بودند. طیف‌سنجی فروسرخ با انعکاس کلی ضعیف شده (ATR-FTIR) ثابت کرد که حین الکتروریسی، هیچ برهمکنش شیمیایی بین مواد تشکیل‌دهنده رخ نداده است و داده‌های کالریمتری روبشی افتراقی (DSC) نشان داد که اسانس به‌خوبی در نانوالیاف محصور شده است. خواص آنتی‌اکسیدانی توسط آزمون DPPH تجزیه و تحلیل شد و کارایی کپسولاسیون برای محافظت از آنتی‌اکسیدان‌ها را تأیید کرد.

کلیدواژه‌ها

موضوعات

  1. Altiok, D., Altiok, E., & Tihminlioglu, F. (2010). Physical, antibacterial and antioxidant properties of chitosan films incorporated with thyme oil for potential wound healing applications. Journal of Materials Science: Materials in Medicine, 21(7), 2227-2236. https://doi.org/10.1007/s10856-010-4065-x
  2. Amjadi, S., Emaminia, S., Davudian, S. H., Pourmohammad, S., Hamishehkar, H., & Roufegarinejad, L. (2019). Preparation and characterization of gelatin-based nanocomposite containing chitosan nanofiber and ZnO nanoparticles. Carbohydrate Polymers, 216, 376-384. https://doi.org/10.1016/j.carbpol.2019.03.062
  3. Bashiri, S., Ghanbarzadeh, B., Ayaseh, A., Dehghannya, J., & Ehsani, A. (2020). Preparation and characterization of chitosan-coated nanostructured lipid carriers (CH-NLC) containing cinnamon essential oil for enriching milk and anti-oxidant activity. LWT, 119, 108836. https://doi.org/10.1016/j.lwt.2019.108836
  4. Bertolo, M. R., Martins, V. C., Horn, M. M., Brenelli, L. B., & Plepis, A. M. (2020). Rheological and antioxidant properties of chitosan/gelatin-based materials functionalized by pomegranate peel extract. Carbohydrate Polymers, 228, 115386. https://doi.org/10.1016/j.carbpol.2019.115386
  5. Cevallos, P. A. P., Buera, M. P., & Elizalde, B. E. (2010). Encapsulation of cinnamon and thyme essential oils components (cinnamaldehyde and thymol) in β-cyclodextrin: Effect of interactions with water on complex stability. Journal of Food Engineering, 99(1), 70-75. https://doi.org/10.1016/j.jfoodeng.2010.01.039
  6. Charernsriwilaiwat, N., Rojanarata, T., Ngawhirunpat, T., Sukma, M., & Opanasopit, P. (2013). Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana International journal of pharmaceutics, 452(1-2), 333-343. https://doi.org/10.1016/j.ijpharm.2013.05.012
  7. Chatterjee, S., Jain, A., & De, S. (2017). Effect of different operating conditions in cloud point assisted extraction of thymol from Ajwain (Trachyspermum Ammi) seeds and recovery using solvent. Journal of food science and technology, 54(13), 4353-4361. https://doi.org/10.1007/s13197-017-2906-z
  8. Dhandayuthapani, B., Krishnan, U. M., & Sethuraman, S. (2010). Fabrication and characterization of chitosan‐gelatin blend nanofibers for skin tissue engineering. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 94(1), 264-272. https://doi.org/10.1002/jbm.b.31651
  9. Ebrahimi, S., Fathi, M., & Kadivar, M. (2019). Production and characterization of chitosan-gelatin nanofibers by nozzle-less electrospinning and their application to enhance edible film’s properties. Food Packaging and Shelf Life, 22, 100387. https://doi.org/10.1016/j.fpsl.2019.100387
  10. Gortzi, O., Lalas, S., Chinou, I., & Tsaknis, J. (2008). Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. European food research and technology, 226(3), 583-590. https://doi.org/10.1007/s00217-007-0592-1
  11. Guinesi, L. S., & Cavalheiro, E. T. G. (2006). The use of DSC curves to determine the acetylation degree of chitin/chitosan samples. Thermochimica Acta, 444(2), 128-133. https://doi.org/10.1016/j.tca.2006.03.003
  12. Haider, A., Haider, S., & Kang, I.-K. (2018). A comprehensive review summarizing the effect of electrospinning parameters and potential applications of nanofibers in biomedical and biotechnology. Arabian Journal of Chemistry, 11(8), 1165-1188. https://doi.org/10.1016/j.arabjc.2015.11.015
  13. Haider, S., Al‐Masry, W. A., Bukhari, N., & Javid, M. (2010). Preparation of the chitosan containing nanofibers by electrospinning chitosan–gelatin complexes. Polymer Engineering & Science, 50(9), 1887-1893. https://doi.org/10.1002/pen.21721
  14. Karim, M., Fathi, M., & Soleimanian-Zad, S. (2020). Incorporation of zein nanofibers produced by needle-less electrospinning within the casted gelatin film for improvement of its physical properties. Food and Bioproducts Processing, 122, 193-204. https://doi.org/10.1016/j.fbp.2020.04.006
  15. Keawchaoon, L., & Yoksan, R. (2011). Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles. Colloids and surfaces B: Biointerfaces, 84(1), 163-171. https://doi.org/10.1016/j.colsurfb.2010.12.031
  16. Kostakova, E., Meszaros, L., & Gregr, J. (2009). Composite nanofibers produced by modified needleless electrospinning. Materials Letters, 63(28), 2419-2422. https://doi.org/10.1016/j.matlet.2009.08.014
  17. Kurd, F., Fathi, M., & Shekarchizadeh, H. (2017). Basil seed mucilage as a new source for electrospinning: Production and physicochemical characterization. International journal of biological macromolecules, 95, 689-695. https://doi.org/10.1016/j.ijbiomac.2016.11.116
  18. Liu, F., Liu, Y., Sun, Z., Wang, D., Wu, H., Du, L., & Wang, D. (2020). Preparation and antibacterial properties of ε-polylysine-containing gelatin/chitosan nanofiber films. International journal of biological macromolecules, 164, 3376-3387. https://doi.org/10.1016/j.ijbiomac.2020.08.152
  19. Moomand, K., & Lim, L. T. (2015). Effects of solvent and n-3 rich fish oil on physicochemical properties of electrospun zein fibres. Food Hydrocolloids, 46, 191-200. https://doi.org/10.1016/j.foodhyd.2014.12.014
  20. Naebe, M., Lin, T., Tian, W., Dai, L., & Wang, X. (2007). Effects of MWNT nanofillers on structures and properties of PVA electrospun nanofibres. Nanotechnology, 18(22), 225605. DOI1088/0957-4484/18/22/225605
  21. Nahr, F. K., Ghanbarzadeh, B., Hamishehkar, H., & Kafil, H. S. (2018). Food grade nanostructured lipid carrier for cardamom essential oil: Preparation, characterization and antimicrobial activity. Journal of Functional Foods, 40, 1-8. https://doi.org/10.1016/j.jff.2017.09.028
  22. Park, W. H., Jeong, L., Yoo, D. I., & Hudson, S. (2004). Effect of chitosan on morphology and conformation of electrospun silk fibroin nanofibers. Polymer, 45(21), 7151-7157. https://doi.org/10.1016/j.polymer.2004.08.045
  23. Piran, F., Khoshkhoo, Z., Hosseini, S., & Azizi, M. (2020). Controlling the antioxidant activity of green tea extract through encapsulation in chitosan-citrate Nanogel. Journal of Food Quality, 2020. https://doi.org/10.1155/2020/7935420
  24. Rezaei, A., Nasirpour, A., & Fathi, M. (2015). Application of cellulosic nanofibers in food science using electrospinning and its potential risk. Comprehensive Reviews in Food Science and Food Safety, 14(3), 269-284. https://doi.org/10.1111/1541-4337.12128
  25. Rezaei, A., Nasirpour, A., Tavanai, H., & Fathi, M. (2016). A study on the release kinetics and mechanisms of vanillin incorporated in almond gum/polyvinyl alcohol composite nanofibers in different aqueous food simulants and simulated saliva. Flavour and Fragrance journal, 31(6), 442-447. https://doi.org/10.1002/ffj.3335
  26. Rodrigues, M. Á. V., Marangon, C. A., Martins, V. d. C. A., & de Guzzi Plepis, A. M. (2021). Chitosan/gelatin films with jatobá resin: Control of properties by vegetal resin inclusion and degree of acetylation modification. International journal of biological macromolecules, 182, 1737-1745. https://doi.org/10.1016/j.ijbiomac.2021.05.160
  27. Shao, P., Niu, B., Chen, H., & Sun, P. (2018). Fabrication and characterization of tea polyphenols loaded pullulan-CMC electrospun nanofiber for fruit preservation. International journal of biological macromolecules, 107, 1908-1914. https://doi.org/10.1016/j.ijbiomac.2017.10.054
  28. Tabatabai, M. B., Mirjalili, M., Yazdiyan, F., & Hekmatimoghaddam, S. (2019). Antibacterial activity and cytotoxicity of nanoliposomic and nanoniosomic essential oil of Trachyspermum copticum. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 89(3), 1109-1116. https://doi.org/10.1007/s40011-018-1025-6
  29. Trifković, K. T., Milašinović, N. Z., Djordjević, V. B., Krušić, M. T. K., Knežević-Jugović, Z. D., Nedović, V. A., & Bugarski, B. M. (2014). Chitosan microbeads for encapsulation of thyme (Thymus serpyllum) polyphenols. Carbohydrate polymers, 111, 901-907. https://doi.org/10.1016/j.carbpol.2014.05.053
  30. Vafania, B., Fathi, M., & Soleimanian-Zad, S. (2019). Nanoencapsulation of thyme essential oil in chitosan-gelatin nanofibers by nozzle-less electrospinning and their application to reduce nitrite in sausages. Food and Bioproducts Processing, 116, 240-248. https://doi.org/10.1016/j.fbp.2019.06.001
  31. Voron’ko, N. G., Derkach, S. R., Kuchina, Y. A., & Sokolan, N. I. (2016). The chitosan–gelatin (bio) polyelectrolyte complexes formation in an acidic medium. Carbohydrate Polymers, 138, 265-272. https://doi.org/10.1016/j.carbpol.2015.11.059
  32. Wu, Y., Luo, Y., & Wang, Q. (2012). Antioxidant and antimicrobial properties of essential oils encapsulated in zein nanoparticles prepared by liquid–liquid dispersion method. LWT-Food Science and Technology, 48(2), 283-290. https://doi.org/10.1016/j.lwt.2012.03.027
  33. Xu, J., Wei, R., Jia, Z., & Song, R. (2020). Characteristics and bioactive functions of chitosan/gelatin-based film incorporated with ε-polylysine and astaxanthin extracts derived from by-products of shrimp (Litopenaeus vannamei). Food Hydrocolloids, 100, 105436. https://doi.org/10.1016/j.foodhyd.2019.105436
  34. Zhang, H., Liang, Y., Li, X., & Kang, H. (2020). Effect of chitosan-gelatin coating containing nano-encapsulated tarragon essential oil on the preservation of pork slices. Meat science, 166, 108137. https://doi.org/10.1016/j.meatsci.2020.108137

Zhu, Y., Bhandari, B., & Prakash, S. (2018). Tribo-rheometry behaviour and gel strength of κ-carrageenan and gelatin solutions at concentrations, pH and ionic conditions used in dairy products. Food Hydrocolloids, 84, 292-302. https://doi.org/10.1016/j.foodhyd.2018.06.016

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