Document Type : Research Article

Authors

1 Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

2 Department of Aqucalture, Marine Science College, Ministry of Higher Education and Inovation, Oman

3 Department of Food Sciene and Technology, Tehran Science and Research Branch, Islamic Azda Unvesity, Tehran, Iran

Abstract

Introduction
 Seaweeds contain a high amount of protein, essential amino acids, vitamins, minerals, unsaturated fatty acids such as arachidonic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), natural pigments, macro and micro nutrient compounds. Microalgae Spirulina (Spirulina platensis) is a species with high nutritional value. About 60% to 70% of the dry weight (Spirulina platensis) is protein, which has all the essential amino acids. This is a cyanobacterial microalga that is cultivated all over the world and used as a supplement in the human diet in the form of tablets, powder and cookies, bread, salad and soup. Several studies have been conducted in the field of investigating the effect of microalgae addition in food products. The purpose of the current research was to investigate the effect of this microalgae powder on sensory, physical, protein and iron properties of three different products of bulk bread, cake and layered sweets with different formulations.
 
Materials and Methods
 Spirulina microalgae dry powder in 0.25%, 0.5%, 0.75%, 1% and 1.25% was added to the formula of three products: bulk bread, layered pastry, and cake. From each product, a sample without microalgae powder was also prepared and considered as a control. The treatments were evaluated in terms of sensory, color, texture, protein and iron content. Sensory evaluation was carried out by 30 panelists using 7 hedonic points to evaluate the color, flavor, texture, smell and overall acceptance. The color of the surface of the samples was done with a Minolta Chroma Meter (CR-300 Minolta Japan). The results calculated based on L* (whiteness/darkness), a*(redness/greenness) and b*(blueness/yellowness). Hardness of samples was measured with Texture Analyzer TA-XT2 (Stable Micro Systems, Surrey, England) and P/0.5 cylindrical probe (12.5 mm diameter) with 30 kg load cell. Protein of the samples was measured by Kjeldahl method and the amount of iron was measured according to the standard method of AOAC 999.11. All analyses were performed in three repetitions and one-way ANOVA and Tukey's test were used to compare the means.
 
 
 
Results and Discussion
 The results showed that the behavior of spirulina microalgae in changing the characteristics of the three products is different, and this difference is especially significant in sensory characteristics. The addition of spirulina microalgae increased the amount of protein and iron in different treatments. This increase for protein in bread, cake and sweets was about 1, 0.6 and 1.2 percent, respectively. Also, the amount of iron in treatments containing microalgae in bread, cake, and layered sweets was 4, 5, and 3 mg/kg, respectively. Spirulina microalgae is basically known as an aquatic plant with high protein and iron. The microalgae used in this research contained a high amount of protein (67.97%) and 29.5 mg/100 grams of iron, so adding this microalga to the samples increased the amount of protein and iron. Sensory evaluation of the samples showed that all three products had an acceptable acceptance score. However, in comparison among the three products of bread, cake and layered sweets, bread had a lower score than the other two products. The instrumental analysis of L*, a*, b* color indices showed that the increase of spirulina caused green color in the treatments and this color change is more significant in the bread sample. Also, the results of texture analysis showed that the addition of spirulina reduces the hardness of samples containing spirulina. It can be concluded that spirulina microalgae can be used to improve texture, color, and also increase the amount of protein and iron in products.

Keywords

Main Subjects

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

  1. AOAC Official Method 999.11. (2006). Determination of lead, cadmium, copper, iron, and zinc in foods atomic absorption spectrophotometry after dry ashing first Action 1999.
  2. (2002). Association of Official Analytical Chemists, 16 Edition, Washington.
  3. Batista, A.P., Raymundo, A., Bandarra, N.M., Sousa, I., Empis, J., & Gouveia L. (2010). Healthier food products with naturally encapsulated functional ingredients–microalgae. 2nd International Conference on Food Innovation, Valencia, October 25-29.
  4. Beheshtipour, H., Haratian, P., Mortazavian, A.M., & Khosravi-Darani, K. (2012). Effects of Chlorella vulgaris and Arthrospira platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties. European Food Research and Technology, 235, 719–728. https://doi.org/10.1007/s00217-012-1798-4
  5. Ciferri, O., & Tiboni, O. (1985). The biochemistry and industrial potential of Spirulina. Annual Review of Microbiology, 39, 503-26. https://doi.org/10.1146/annurev.mi.39.100185.002443
  6. Danesi, E., Navacchi, M., Takeuchi, K., Frata, M., Carlos, J., & Carvalho, M. (2010(. Application of Spirulina platensis in protein enrichment of Manico based bakery products. Journal Biotechnology, 150(311), DC, USA.
  7. Denka, Z., & Rosen, C. (2019). Effect of Spirulina platensis on the crumb firming of wheat bread during storage. Food Technology, 851-859. https://doi.org/10.24263/2304-974X-2019-8-4-15
  8. Fradique, M., Batista, A., Nunes, M., Gouveia, L., Bandarra, N., & Raymundo A. (2010(. Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: Preparation and Journal of the Science of Food and Agriculture, 90(10), 1656–64.https://doi.org/10.1002/jsfa.3999
  9. Hoseini, S.M., Khosravi-Darani, K,. & Mozafari, M.R. (2013). Nutritional and medical applications of spirulina microalgae. Mini-Reviews in Medicinal Chemistry, 13, 1231-1237. https://doi.org/10.2174/1389557511313080009
  10. Gouvei, L., Raymundo, A., Batista, A., Sousa, I., & Empis, J. (2005 .(Chlorella vulgaris and Haematococcus pluvialis biomass as colouring and antioxidant in food emulsions. European Food Research and Technology, 8, 433-436. https://doi.org/1007/s00217-005-0105-z
  11. Mamathaa, B.S., Namithab, K.K., Amudha, S., Smithac, J.G.A., & Ravishankar, G.A. (2007). Studies on use of Enteromorpha in snack food. Food Chemistry, 101, 1707–1713. https://doi.org/11016/j.foodchem.2006.04.032
  12. Maria, C.M., Francesca, Cuomo, L., Falasca, M., Carmela, T., Elisa De, A., & Emanuele, M. (2021). Nutritional and technological quality of high protein Pasta. Foods, 10(589), 1-11. https://doi.org/10.3390/foods10030589
  13. Mostolizade, S., Moradi, Y., Mortazavi, M.S., Motalbi, A.A., & Ghaeni, M. (2020). Effects of incorporation Spirulina platensis (Gomont, 1892) powder in wheat flour on chemical, microbial and sensory properties of pasta. Iranian Journal of Fisheries Sciences, 19(1), 410-420. https://doi.org/10.22092/ijfs.2019.119107
  14. Nikouzad, H.T.A., & Azizi, M .(2011). Effect of adding oat bran on rheological properties of bread and quality of Sangak bread. Iranian Journal of Food Science and Technology, 8(1), 1-10.
  15. Powell, R., Nevels, E., & McDowell, M. (2011). Algae Feeding in Human. Journal Nutrition, 75, 7-12. https://doi.org/10.1093/jn/75.1.7
  16. Prabhasankar, P., Ganesan, P., & Bhaskar, N. (2009). Influence of Indian brown seaweed (Sargassum marginatum) as an ingredient on quality, biofunctional and microstructure characteristics of pasta. Food Science and Technology International. https://org/10.1177/1082013209350267
  17. Rafael, G., Tyri, V., Kolbrun, S., & Helgi, T. (2004). Effects of rearing temperature and strain on sensory characteristics, texture, color and fat of Arctic charr (Salvelinus alpinus). Journal of Food Quality and Preference, 15, 177-185. https://doi.org/1016/S0950-3293(03)00056-9
  18. Salehifar, M., Shahbazizadeh, S., Khosravi-Darani Hosravi-Darani, K., Behmadi, H., & Ferdowsi, R. (2013). Possibility of using microalgae Spirulina platensis powder in industrial production of Iranian traditional cookies. Iranian Journal of Nutrition Sciences and Food Technology, 7, 63-72.
  19. Sanjari, S., Sarhadi, H., & Shahdadi, F. (2018). Investigating the effect of Spirulina platensis microalgae on textural and sensory properties of baguette bread. Journal of Nutrition and Food Security (JNFS), 4, 218-225.

 

 

CAPTCHA Image