Effects of Lavender Essential Oil Addition on Oxidative Stability of Canola Oil under Accelerated Condition

Document Type : Research Article

Authors

• Fatemeh Sadat Khanagaei ¹
• Fateme Akrami Mohajeri ¹
• Elaheh Askari ²
• Hossein Fallahzadeh ³
• Elham Khalili Sadrabad ⁴

¹ Research Center for Food Hygiene and Safety, Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

² Department of Nutrition, School of Health & Nutrition, Lorestan University of Medical Sciences, Khoram abad, Iran

³ Center for Healthcare Data Modeling, Departments of Biostatistics and Epidemiology, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

⁴ Research Center for Food Hygiene and safety, Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

https://doi.org/10.22067/ifstrj.2024.83911.1276

Abstract

Introduction
Canola oil with high unsaturated fatty acids and nutritional value is susceptible to oxidation due to lipid oxidation. Lipid oxidation leads to a reduction of nutritional quality, sensory and safety characteristics of the vegetable oils. To retard lipid oxidation, the synthetic antioxidants are usually used in the vegetable oils. By increasing the public concern about health problems of synthetic antioxidants, the use of natural antioxidants is increasing. Lavender (Lavandula officinalis) is an evergreen plant native to the Mediterranean. The presence of linalool, linalyl acetate, 1,8-cineole B-ocimene, terpinen-4-ol, and camphor in lavender essential oil, make it a good natural antioxidant which could use in food industry. Therefore, in the current research, it was aimed to investigate the antioxidant effect of lavender essential oil on the stability of canola oil.
 
Material and Method
The lavender was bought from Golestan province and dried in room temperature. The lavender essential oil was prepared by hydro distillation of flower heads. Then, the phenolic compounds were determined using GC-MASS. The Total phenolic content (TPC), flavonoid content (TFC), and antioxidant activity (FRAP and DPPH) of lavender essential oil were evaluated. Then, lavender essential oil in concentrations of 200, 400, 600, 800, and 1000 mg/kg was added to the crude canola oil compared to canola oils without antioxidants and synthetic antioxidant TBHQ (100 and 200 mg/kg). Then, the samples were kept at 60 to 70 ^oC for 12 days. The analysis was done in an interval of 24 h for 12 days. Lipid oxidation of samples was determined by peroxide value, p-anisidine value, TOTOX value, and thiobarbituric acid each 24 h. analyses of Data were done by one-way analysis of variance (ANOVA) using SPSS software and the means were compared by the Tukey multiple range test.
 
Result and Discussion
 According to the GC-MS analysis, 1, 8-cineole (59.45 %), linalool acetate (32.48 %), linalool (6.31 %), and limonene (1.06 %) were identified as the major constituent of lavender essential oil. Also, Total phenol, flavonoid, FRAP and DPPH (IC₅₀) contents of lavender essential oil were 71.55 mg GAE/g, 82.66 mg of rutin/100 g, 12.63 mmol H₂SO₄, and 55.88 mg/ml, respectively. According to the results, all lipid oxidation indexes were increased after twelve days of storage. In general, lavender essential oil was effective in retarding the oxidation of canola oil at a temperature of 70 ^oC. Also, the concentration of 1000 mg/kg of the essential oil had antioxidant activities similar to the TBHQ in 100 mg/kg concentration.
 
Conclusion
 It was showed that lavender essential oil, as a natural antioxidant, has the ability to react with the radicals resulting from the oxidation of lipids and causes the interruption of oxidation chain reactions and increases the time and decreases the rate of oxidation. As observed, the oxidation of canola oil in all samples, especially the samples without antioxidants or antioxidants to a lesser extent, increased significantly with increasing storage time. In general, lavender essential oil at L₁₀₀₀ concentration and also in some oxidation indices of lavender essential oil at L₈₀₀ concentration has an effective role in preventing the oxidation of canola oils like synthetic antioxidant TBHQ.

Keywords

• Antioxidant activity
• Canola oil
• Lavender essential oil
• Lipid oxidation

Main Subjects

• Food Chemistry

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

References

1. Aachary, A.A., Chen, Y., Eskin, N.M., & Thiyam‐Hollander, U. (2014). Crude canolol and canola distillate extracts improve the stability of refined canola oil during deep‐fat frying. European Journal of Lipid Science and Technology, 116(11), 1467-1476. https://doi.org/10.1002/ejlt.201300498
2. Abdulkarim, S., Long, K., Lai, O.M., Muhammad, S., & Ghazali, H. (2007). Frying quality and stability of high-oleic Moringa oleifera seed oil in comparison with other vegetable oils. Food Chemistry, 105(4), 1382-138. https://doi.org/10.1016/j.foodchem.2007.05.013
3. Adaramola, B., Onigbinde, A., & Shokunbi, O. (2016). Physiochemical properties and antioxidant potential of Persea Americana seed oil. Chemistry International, 2(3), 168-175.
4. Amiri, M., Arab, M., Sadrabad, E.K., Mollakhalili-Meybodi, N., & Fallahzadeh, H. (2023). Effect of gamma irradiation treatment on the antioxidant activity, phenolic compounds and flavonoid content of common buckwheat. Radiation Physics and Chemistry, 111127. https://doi.org/10.1016/j.radphyschem. 111127
5. Badr, M.M., Badawy, M.E., & Taktak, N.E. (2021). Characterization, antimicrobial activity, and antioxidant activity of the nanoemulsions of Lavandula spica essential oil and its main monoterpenes. Journal of Drug Delivery Science and Technology, 65, 102732.
6. Bajalan, I., Mohammadi, M., Alaei, M., & Pirbalouti, A.G. (2016). Total phenolic and flavonoid contents and antioxidant activity of extracts from different populations of lavandin. Industrial Crops and Products, 87, 255-260. https://doi.org/10.1016/j.indcrop.2016.04.059
7. Behbahani, B., Shahidi, F., Yazdi, F.T., Mortazavi, S.A., & Mohebbi, M. (2017). Use of Plantago major seed mucilage as a novel edible coating incorporated with Anethum graveolens essential oil on shelf life extension of beef in refrigerated storage. International Journal of Biological Macromolecules, 94, 515-526. https://doi.org/10.1016/j.ijbiomac.2016.10.055
8. Bensmira, M., Jiang, B., Nsabimana, C., & Jian, T. (2007). Effect of lavender and thyme incorporation in sunflower seed oil on its resistance to frying temperatures. Food Research International, 40(3), 341-346. https://doi.org/10.1016/j.foodres.2006.10.004
9. Caser, M., Falla, N.M., Demasi, S., & Scariot, V. (2023). From fresh to dried lavender flower: Changes in phytochemical profile according to drying mHorticulturae, 9(6), 700. https://doi.org/10.3390/ horticulturae9060700
10. Ciocarlan, A., Lupascu, L., Aricu, A., Dragalin, I., Popescu, V., Geana, E.-I., & Hristozova, G. (2021). Chemical composition and assessment of antimicrobial activity of lavender essential oil and some by-products. Plants, 10(9), 1829.
11. Cui, L., Cho, H.T., McClements, D.J., Decker, E.A., & Park, Y. (2016). Effects of salts on oxidative stability of lipids in Tween-20 stabilized oil-in-water emulsions. Food Chemistry, 197, 1130-1135. https://doi.org/1016/j.foodchem.2015.11.099
12. Dakhlaoui, S., Wannes, W.A., Sari, H., Hmida, M.B., Frouja, O., Limam, H., & Jallouli, S. (2022). Combined effect of essential oils from Lavender (Lavandula officinalis) aerial parts and coriander (Coriandrum sativum L.) seeds on antioxidant, anti-diabetic, anti-cancer and anti-inflammatory activities. Journal of Essential Oil Bearing Plants, 25(1), 188-199. https://doi.org/10.1080/0972060X.2022.2049892
13. de Rapper, S., Kamatou, G., Viljoen, A., & van Vuuren, S. (2013). The in vitro antimicrobial activity of Lavandula angustifolia essential oil in combination with other aroma-therapeutic oils. Evidence-Based Complementary and Alternative Medicine. https://doi.org/1155/2013/852049
14. Delfanian, M., Esmaeilzadeh Kenari, R., & Sahari, M.A. (2015). Antioxidative effect of loquat (Eriobotrya japonica) fruit skin extract in soybean oil. Food Science & Nutrition, 3(1), 74-80. https://doi.org/10.1002/fsn3.193
15. Dif, M., Benyahia, M., Toumi Benali, F., Rahmani, M., & Bouazza, S. (2017). Phenolic content and antioxidant activity of three algerian species of lavenders. Phytothérapie, 15(6), 367-372. https://doi.org/10.1007/s10298-016-1028-5
16. Dong, G., Bai, X., Aimila, A., Aisa, H.A., & Maiwulanjiang, M. (2020). Study on lavender essential oil chemical compositions by GC-MS and improved pGC. Molecules, 25(14), 3166. https://doi.org/3390/molecules25143166
17. Farhoosh, R., Pazhouhanmehr, S., & Poorazrang, H. (2010). Heat stability of the oils from current canola cultivars in Iran. Iranian Journal of Food Science and Technology, 6(4), 11-17.
18. Foglia, T.A., Petruso, K., & Feairheller, S.H. (1993). Enzymatic interesterification of tallow sunflower oil mixture Journal of the American Oil Chemists' Society, 70(3), 281-285. https://doi.org/10.1007/BF02545309
19. Fu, M., Qu, Q., Yang, X., & Zhang, X. (2016). Effect of intermittent oven drying on lipid oxidation, fatty acids composition and antioxidant activities of walnut. LWT-Food Science and Technology, 65, 1126-1132. https://doi.org/10.1016/j.lwt.2015.10.002
20. Ghadri, T.A., Mousavi Gargari, S.L., Sharafi, S.M., Darvish Alipour Astaneh, S., & Rezaei, M.B. (2010). Antimicrobial, antioxidant, hematologic and cytotoxic properties of Lavandula angustifolia essential o Pathobiology Research (Modares Journal Of Medical Sciences), 12(4), 45-58.
21. Goli, A.H., Barzegar, M., & Sahari, M.A. (2005). Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chemistry, 92(3), 521-525. https://doi.org/10.1016/ foodchem.2004.08.020
22. Karami, O.R., Khodaverdi, M., & ALI, A.F. (2010). Antibacterial effect of effective compounds of Satureja hortensis and Thymus vulgaris essential oils against Erwinia amylovora. https://jast.modares.ac.ir/article-23-2947-en.html
23. Marín, I., Sayas-Barberá, E., Viuda-Martos, M., Navarro, C., & Sendra, E. (2016). Chemical composition, antioxidant and antimicrobial activity of essential oils from organic fennel, parsley, and lavender from Spain. Foods, 5(1), 18. https://doi.org/3390/foods5010018
24. Mastrodi Salgado, J., Baroni Ferreira, T.R., de Oliveira Biazotto, F., & dos Santos Dias, C.T. (2012). Increased antioxidant content in juice enriched with dried extract of pomegranate (Punica granatum) peel. Plant Foods for Human Nutrition, 67(1), 39-43. https://doi.org/1007/s11130-011-0264-y
25. Mohammadi, M., Hajeb, P., Seyyedian, R., Hossein Mohebbi, G., & Barmak, A. (2013). Evaluation of oxidative quality parameters in imported edible oils in Iran. British Food Journal, 115(6), 789-795. https://doi.org/10.1108/BFJ-Feb-2011-0035
26. Naghshineh, M., Ariffin, A.A., Ghazali, H.M., Mirhosseini, H., Kuntom, A., & Mohammad, A.S. (2009). Monitoring the change patterns of physicochemical properties of oil blend as function of storage time. Journal of Food, Agriculture & Environment, 7(3&4), 1-2 https://www.isfae.org/scientificjournal.php
27. Rodrigues, N., Malheiro, R., Casal, S., Manzanera, M.C.A.-S.-., Bento, A., & Pereira, J.A. (2012). Influence of spike lavender (Lavandula latifolia) essential oil in the quality, stability and composition of soybean oil during microwave heating. Food and Chemical Toxicology, 50(8), 2894-2901. https://doi.org/10.1016/j.fct.2012.05.020
28. Sarafraz Ardakani, M., Akrami Mohajeri, F., Askari, E., Javdan, G., Pourramezani, F., Fallahzadeh, H., & Khalili Sadrabad, E. (2023). Efficacy of different Sesame oil refining stages in reduction of heavy metals, antioxidant activity, and oxidative pJournal of Food Processing and Preservation. https://doi.org/10.1155/2023/1197398
29. Sarrami, S., Akrami Mohajeri, F., Sadeghizadeh-Yazdi, J., Jambarsang, S., & Khalili Sadrabad, E. (2023). Chemical composition and antioxidant activity of clove essential oil and its effect on stability of sesame oil under accelerated cJournal of Nutrition and Food Security, 8(3), 343-352. https://doi.org/10.18502/jnfs.v8i3.13280
30. Sayyad, R., & Farahmandfar, R. (2017). Influence of Teucrium polium essential oil on the oxidative stability of canola oil during storage. Journal of Food Science and Technology, 54, 3073-3081. https://doi.org/10.1007/s13197-017-2743-0
31. Sharayei, P., Farhoosh, R., Poorazrang, H., & Khodaparast, M.H.H. (2011). Effect of bene kernel oil on the frying stability of canola oil. Journal of the American Oil Chemists' Society, 88, 647- https://doi.org/10.1007/s11746-010-1708-5
32. Taha Nejad, M., Barzegar, M., Sahari, M., & H, N.B. (2012). Evaluation of antioxidant activity of Lavandula angustifolia essential oil in crude soybean oil s Journal Medicinal Plants, 11(41), 127-140. https://jmp.ir/article-1-482-fa.html
33. Turan, (2014). Efficiency of various plant essential oils in stabilization of canola oil and of its purified triacylglycerols. Journal of Essential Oil Research, 26(3), 166-176. https://doi.org/10.1080/10412905.2013.840810
34. Viuda-Martos, M., Mohamady, M., Fernández-López, J., Abd ElRazik, K., Omer, E., Pérez-Alvarez, J., & Sendra, E. (2011). In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control, 22(1), 1715-1722. https://doi.org/10.1016/j.foodcont.2011.04.003
35. Xylia, P., Chrysargyris, A., Botsaris, G., & Tzortzakis, N. (2017). Potential application of spearmint and lavender essential oils for assuring endive quality and safety. Crop Protection, 102, 94-103. https://doi.org/10.1016/j.cropro.2017.08.015
36. Yalcin, H., Kavuncuoğlu, H., Tulukcu, E., & Eroğlu, Z. (2017). The effect of harvest time on the bioactive properties and volatile components of lavender (Lavandula officinalis). Quality Assurance and Safety of Crops & Foods, 9(3), 275-283. https://doi.org/10.3920/QAS2015.0763