Arash Koocheki; Seyed Ali Mortazavi
Abstract
In order to prevent the turbidity during storage of kiwifruit juice, clarification is necessary. Through proper clarification, flavor and taste of the product is also improved. Therefore, the purpose of the present study was to present a method to determine the optimum amount of clarifying agents (Pectinase ...
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In order to prevent the turbidity during storage of kiwifruit juice, clarification is necessary. Through proper clarification, flavor and taste of the product is also improved. Therefore, the purpose of the present study was to present a method to determine the optimum amount of clarifying agents (Pectinase enzyme, Bentonite and polyvinyl poly pyrolidone) for producing kiwifruit juice with stable clarity. For this purpose, effect of Pectinase enzyme (2-8 g/kg), Bentonite (200-350 g/Ton) and polyvinyl poly pyrolidone (70-110 g/100 lit) on viscosity, turbidity, clarity and total phenolics compound of kiwifruit juice was investigated. For each response a second order polynomial model was developed using a multiple linear regression analysis. Results showed that R2 for viscosity, turbidity, clarity and the total amount of phenolics were more than 0.90. The statistical analysis indicates that viscosity, turbidity and clarity (P
Abbas Ahmadi; Seyed Ali Mortazavi; Elnaz Milani; Reza Rezaeemokaram
Abstract
In this study, frozen yoghurt was produced as a synbiotic product. A food product containing both probiotics and prebiotics is named as synbiotic or functional food. Lactobacillus acidophilus (La-5) as a probiotic bacteria was added to frozen yoghurt in two types; free and encapsulated, and its survivability ...
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In this study, frozen yoghurt was produced as a synbiotic product. A food product containing both probiotics and prebiotics is named as synbiotic or functional food. Lactobacillus acidophilus (La-5) as a probiotic bacteria was added to frozen yoghurt in two types; free and encapsulated, and its survivability was evaluated during 60 days storage at -18 °C . Also Fructo-oligosaccharide as a prebiotic compound was used for producing frozen yoghurt in different levels (0%, 0.4% and 0.8% (w/w)). The viable cell number in free state in samples with (0%, 0.4% and 0.8% (w/w)) Fructo-oligosaccharide was 3.8×109 cfu/ml, 3.5×109 cfu/ml and 3.8×109 cfu/ml and after 60 days of storage these numbers were decrease to 2×107 cfu/ml, 2.2×107 cfu/ml and 2.2×107 cfu/ml respectively. Whereas in encapsulated state, the viable cells in samples with (0%, 0.4% and 0.8% (w/w)) Fructo-oligosaccharide these numbers was 7.5×109 cfu/ml, 8.9×109 cfu/ml and 9.8×109 cfu/ml and after 60 days, these numbers were decreased to 2.13×109 cfu/ml, 2.5×109 cfu/ml and 2.9×109 cfu/ml.The results indicated that encapsulation of L. acidophilus could significantly (p
Elnaz Milani; Hajar Naeemi; Seyed Ali Mortazavi; Arash Koocheki
Abstract
In this study, symbiotic yog-ice (rozen yogurt) was produced that was inoculated with different levels of inulin(0,2.5 amd 5%)as prebiotic compound and Lactobacillus Casei as probiotic bacteria in two types; free and encapsulated with Sodium Algint-Whey Protein Concentration for low fat yog-ice cream.The ...
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In this study, symbiotic yog-ice (rozen yogurt) was produced that was inoculated with different levels of inulin(0,2.5 amd 5%)as prebiotic compound and Lactobacillus Casei as probiotic bacteria in two types; free and encapsulated with Sodium Algint-Whey Protein Concentration for low fat yog-ice cream.The survivability of this probiotic bacteria was evaluated under simulated gastro-intestinal conditions ,during 30 days storage at -18oC. In the samples with free state of probiotic bacteria, the number of viable cells ,after 1h exposuring simulated gastric juice(pH=2,pepcine) decreased from 9.78-9.81 log cfu/ml to 6.97-7.26 log cfu/ml,at the end of 30 days storage at -18oC,while, the viable number of encapsulated state of probiotic bacteria, after 1h exposuring simulated gastric juice, decreased from 8.15-8.66 log cfu/ml to 6.80-7.09 log cfu/ml at the end of 30 days storage at -18oc.After exposuring simulated gastric juice, samples was ezposured 2.5h under simulated intestinal juice(pH=7.4,panceratin), and the results that was gained showed that, the samples containing free probiotic bacteria after 2.5h exposuring simulated intestinal juice decreased from 8.808-9.355 log cfu/ml to 5.159-5.9 log cfu/ml at the end of 30 days storage at -18oC.While, in the samples containing sodium alginate-whey protein concentration capsules, the viable number of cells decreased from 7.38-8.201 log cfu/ml to 6.151-6.672 log cfu/ml, at the end of 30 days storage at -18oC. The results indicate that there are significant differences (p
Ashraf Nazarian; Seyed Ali Mortazavi; Majid Arab Ameri; Marzieh Bolandi; Mohammad Mahdi Karimkhani
Abstract
Recently great afford have focused on the production of food and beverages which are rich in phenolic and antioxidant components. The epidemiology investigations have proved that the regular Composition of food and beverages are richer in phenolic and antioxidant components can strongly decrease the ...
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Recently great afford have focused on the production of food and beverages which are rich in phenolic and antioxidant components. The epidemiology investigations have proved that the regular Composition of food and beverages are richer in phenolic and antioxidant components can strongly decrease the cardiovascular disease. This was done after the preparation of components such as soymilk mixed with sour cherry and barberries juice. In this research, the DPPH free radical method bas been used in order to analyses the antioxidant activity, Folin-Ciocalteau and pH differential method were to estimate the amounts of phenolic compounds and antioxidant and anthocyanin . Also the amount of vitamin c was estimated by standard method which is used for fruit juice . The data's were analyzed by SAS software . The maximum scavenging capacity of radical DPPH wsa seen in sample (20 %soymilk and 80% juice ) with the average of 41. 88 %SC (ppm) ,85. 5 mg/100ml of anthocyanin content,10. 12 mgGAE /gr dry extract phenolic contents and 4. 192 mg/100ml of vitamin c were reported which showed reduction in order samples . corrolational researches shows appositive and significant relation ship between the phenolic contents and anthocyanin and vitamin c of compound and their ability in neutralization of DPPH radical.
Seyed Hossein Razavizadegan Jahromi; Farideh Tabatabaei Yazdi; Mahdi Karimi; Seyed Ali Mortazavi
Abstract
Efforts to improve the quality of bakery products are increasing every day. Several techniques were developed for this purpose. In this study the possibility of betterment and optimization of quality properties of dough and Barbari bread with emphasis on process variables using rotatable response surface ...
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Efforts to improve the quality of bakery products are increasing every day. Several techniques were developed for this purpose. In this study the possibility of betterment and optimization of quality properties of dough and Barbari bread with emphasis on process variables using rotatable response surface methodology were investigated. The independent process variables for baking process were dough mixing time at low speed (MTLS) (63 rpm) and high speed (MTHS) (180 rpm) from 2 to 8 min and dependent process variables were dough adhesiveness, dough cohesiveness, dough hardness, dough resilience, dough stickiness and specific volume of Barbari bread. Statistical results showed that all the process variables have a significant effect on all the responses. The linear terms of MTHS was the most effective factor on dough stickiness and dough resilience. Quadratic terms of MTHS and MTLS were the most effective factor on dough cohesiveness and dough hardness and specific volume of Barbari bread, respectively. Interaction terms of process variables have significant effect (p
Elham Zaerzadeh; Seyed Ali Mortazavi; Mahmoud Reza Jafari; Sima Afsharnejad; Farideh Tabatabaei Yazdi; Mehdi Nasiri mahalati
Abstract
The objective of this study was evaluating the effectiveness of encapsulated nisin in liposoms in contrast to free nisin in control of Listeria monocytogenes ATCC 19117 in Feta cheese during its ripening. The size of the nano-encapsules with nisin was around 103-150 nm and of the nano-encapsules without ...
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The objective of this study was evaluating the effectiveness of encapsulated nisin in liposoms in contrast to free nisin in control of Listeria monocytogenes ATCC 19117 in Feta cheese during its ripening. The size of the nano-encapsules with nisin was around 103-150 nm and of the nano-encapsules without nisin was of approximately 101-143 nm. Addition of 500IU/g nisin to cheese resulted into 0.57, 4 and 3.7 log reduction in viable cells, respectively in free nisin, nano-encapsulated nisin (formulation 1) and nano-encapsulated nisin (formulation 2) at the end of four weeks ripening. In addition, changes in pH during this period of time was also affected by the form of addition of nisin, it was significantly different from liposomal nisin formulations(p
Elnaz Milani; Homa Baghaei; Seyed Ali Mortazavi
Abstract
Yog-ice cream (Frozen yogurt), is a kind of frozen dessert which has similar features to ice cream in physical and appearance characteristics. Recently, there has been a growing interest in using frozen yogurt due to existence of lactic acid bacteria & fermentation processing. Besides, yog-ice cream ...
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Yog-ice cream (Frozen yogurt), is a kind of frozen dessert which has similar features to ice cream in physical and appearance characteristics. Recently, there has been a growing interest in using frozen yogurt due to existence of lactic acid bacteria & fermentation processing. Besides, yog-ice cream contains lower levels of sugar, fat & emulsifiers than ice cream. This Research was conducted to examine the effect of date syrup with different concentrations (0, 25 and 50% as a sugar replacement) & guar gum addition (0, 0.1, 0.2 & 0.3% as a fat replacer) on rheological properties of low fat orange yog-ice cream. The results showed that by increasing the date syrup and guar concentration, the viscosity of mixture increased but overrun decreased significantly in all samples (p< 0.05). Results for stickiness of the yog-ice cream samples indicated that the low fat yog-ice cream was considerably less adhesive/sticky than both low fat product containing guar and date syrup. Hardness of low fat yog-ice cream samples was observed to be considerably higher than the samples with guar and date syrup, whilst the addition of guar and date syrup up to 0.3% & 25%, decreased the hardness of the low fat product.
Keywords: Low-fat yog-ice cream, Guar, Dates syrup, Rheological properties
Arash Koocheki; Fakhri Shahidi; Seyed Ali Mortazavi; Mahdi Karimi; Elnaz Milani
Abstract
The effect of xanthan and Qodume shirazi seed gum on fresh bread quality and its influence on dough rheology were studied. Incorporation of 0.3 to 1% of these two gums was investigated to evaluate its effect on the Mixing tolerance index (MTI), dough development time (DDT), gelatinization temperature ...
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The effect of xanthan and Qodume shirazi seed gum on fresh bread quality and its influence on dough rheology were studied. Incorporation of 0.3 to 1% of these two gums was investigated to evaluate its effect on the Mixing tolerance index (MTI), dough development time (DDT), gelatinization temperature (GT), viscosity, dough stability and quality of wheat flour. Addition of xanthan and Qodume shirazi seed gum increased the water absorption, viscosity and dough stability. In contrast, MTI and GT decreased with increase in gum concentration. DDT remained statistically unaffected by all the levels of gum. There was increase in softness of bread with increase in the level of these gums up to 1%. Hardness values indicated that the breads containing xanthan gum was softer than the one with Qodume shirazi seed gum. However, Qodume shirazi seed gum could also improve these qualities so that it could be used as a new source of hydrocolloid in bread.
Keywords: Bread, Gum, Alyssum homolocarpum seeds, Rheology, Hardness
Rassoul Kadkhodaee; Seyed Ali Mortazavi; Farideh Tabatabaei Yazdi
Abstract
The aim of this work was to investigate the influence of interactions between modified starch (Hi-Cap 100) and the nonionic surfactant Tween 80 on the properties of cardamom oil-in-water emulsion and its corresponding spray dried microcapsules. To this end the mean diameter and size distribution of droplets ...
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The aim of this work was to investigate the influence of interactions between modified starch (Hi-Cap 100) and the nonionic surfactant Tween 80 on the properties of cardamom oil-in-water emulsion and its corresponding spray dried microcapsules. To this end the mean diameter and size distribution of droplets as well as their polydispersity index and also the viscosity and flow behavior of emulsion samples along with the encapsulation efficiency of powders were determined in the presence of various ratios of Tween 80. The results showed that increasing the concentration of Tween 80 up to 1% decreased the size of droplets and their polydispersity index while further increase beyond this point led to adverse effect. Also significant rise in the viscosity and marked alteration in the flow behaviour of emulsions was observed within this limit, which clearly indicates the influence of Hi-Cap 100 and Tween 80 interaction on the quality attributes of samples. Moreover, the findings of this study demonstrated that the aforementioned interaction considerably affected the microstructure and overall encapsulation efficiency of spray dried powders.
Keywords: Emulsion, Microencapsulation, Surfactant, Modified starch, Hi-Cap 100, Tween 80
Mohsen Qods Rohani; Seyed Ali Mortazavi; Mostafa Mazaheri Tehrani
Abstract
In this study, ultrafiltrated Feta cheese was prepared from cow’s milk ultrafiltraton retentate and soymilk blend. Rennet and calcium chloride were used simultaneously as coagulant to coagulate cow’s milk proteins and soymilk proteins, respectively. Then, the effect of storage period on physical ...
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In this study, ultrafiltrated Feta cheese was prepared from cow’s milk ultrafiltraton retentate and soymilk blend. Rennet and calcium chloride were used simultaneously as coagulant to coagulate cow’s milk proteins and soymilk proteins, respectively. Then, the effect of storage period on physical (Texture Profile Analysis test, Penetration test), chemical (total solids, protein, fat) and sensory (flavor, texture) properties of samples was investigated in 3, 20, 40 and 60 days after production. The results showed that during storage period physical properties decreased for both Texture Profile Analysis test and Penetration test. But, sensory and chemical characteristics of the product during storage did not significantly change, the way that the flavor and texture scores of the last day were still higher than the middle and the product after two months storage well was usable.
Keywords: Soymilk, Retentate, CaCl2, Storage period, Cheese
Fatemeh Zabetian Hosseini; Seyed Ali Mortazavi; Bibi Sedigheh Fazli Bazaz; Arash Koocheki; Shadi Bolourian
Abstract
Thymus vulgaris is a plant from Lamiasceae family. The antimicrobial activities of T. vulgaris essential oil and extract depend on the phenolic compounds like carvacrol, thymol, γ-terpinene and p-cymene, which are reported to have antibacterial properties. In this research the antimicrobial effect of ...
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Thymus vulgaris is a plant from Lamiasceae family. The antimicrobial activities of T. vulgaris essential oil and extract depend on the phenolic compounds like carvacrol, thymol, γ-terpinene and p-cymene, which are reported to have antibacterial properties. In this research the antimicrobial effect of different concentration (0, 0.1, 0.2, 0.4%)of Thymus vulgaris extract was studied on Salmonella enteritidise PT4 presence mayonnaise at two different temperatures (4oC and 25oC) during storage time. This test was evaluated as a completely randomized factorial design with 3 replicates and the models were shown using RSM. The results showed that the antimicrobial effect of T.vulgaris extract was higher for the mayonnaise kept at 25oC. This effect increased with increase in concentration of extract. These concentrations of T.vulgaris extract didn’t have any unfavorable effect on the sensory parameters in mayonnaise.
Keywords: Mayonnaise, T. vulgaris extract, Salmonella enteritidise, Modeling
Rassoul Kadkhodaee; Seyed Ali Mortazavi
Abstract
In this study formation and properties of o/w emulsion stabilized by a mixture of sodium caseinate (2, 5 and 10 wt %) and non-ionic emulsifier Tween 80, sorbitan mono-9-octadecenoate, (0, 0.5, 1 and 1.5 wt %) were investigated at various pH values(3, 5 and 7) and calcium ion concentrations (0, 0.2, 0.4 ...
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In this study formation and properties of o/w emulsion stabilized by a mixture of sodium caseinate (2, 5 and 10 wt %) and non-ionic emulsifier Tween 80, sorbitan mono-9-octadecenoate, (0, 0.5, 1 and 1.5 wt %) were investigated at various pH values(3, 5 and 7) and calcium ion concentrations (0, 0.2, 0.4 and 0.6 wt%). Emulsion samples were prepared by using a sonicator with a nominal power output of 750 W and an operating frequency of 20 kHz. Sonication of samples was carried out in a temperature-controlled chamber at a constant temperature of 20 ºC and the highest acoustic power available for 3 minutes. The results showed that increasing the concentration of sodium caseinate and Tween 80 generally reduced the size of droplets, although it was observed that Tween80 adversely affected the size of droplets at higher concentration under acidic conditions. Moreover, pH and calcium ion significantly affected the diameter and specific surface area of droplets (p
Nasser Valipoor Motlagh; Mohamad Taghi Hamed Mosavian; Seyed Ali Mortazavi
Abstract
In this research, attention is focused on the resistantance of packages containing silver nanoparticles to gases such as oxygen as well as to the antimicrobial property of these packages. The effect of these packages on increasing quality and shelf time of the barberry was investigated in comparison ...
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In this research, attention is focused on the resistantance of packages containing silver nanoparticles to gases such as oxygen as well as to the antimicrobial property of these packages. The effect of these packages on increasing quality and shelf time of the barberry was investigated in comparison with the current polyethylene packages. To do this, different microbial factors and the color of the barberry were investigated. The microbial experiments were done according to the national standards of Iran. The data obtained from microbial tests statistically considered by SPSS15 software. They showed decreasing microbial capacity, especially in the case of mold and counting total bacteria, packages containing silver nanoparticles had between 1% and 2% concentration of nanoparticles in comparison with the samples of polyethylene packages. Investigation of the apparent color of the samples was also done according to an average red color and brightness of the images taken from barberry seeds by Photoshop CS3 software, version10. The data obtained from these investigations statistically considered by SPSS15 software. The results showed that the apparent color is qualitatively better in the samples of packages containing 2% and 3% silver nanoparticles, compared with samples of polyethylene packages. However, a low concentration of nanoparticles, in the range of 200 part per million (ppm), did not produce a great effect on increasing either the quality or the shelf life in barberry, compared with ordinary polyethylene packages.
Keywords: Barberry, Nanosilver package, Silver nanoparticles, Microorganism, Antimicrobial property, Polyethylene
Behzad Nasehi; Seyed Ali Mortazavi; Seyed Mohammad Ali Razavi
Abstract
Abstract
The aim of this research was to investigate the enthalpy variations of Iranian flat breads during storage in room temperature and determining relationship between enthalpy and amount of staling development. For this purpose, the major Iranian Breads including Barbari, Taftoon, Lavash and Sangak ...
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Abstract
The aim of this research was to investigate the enthalpy variations of Iranian flat breads during storage in room temperature and determining relationship between enthalpy and amount of staling development. For this purpose, the major Iranian Breads including Barbari, Taftoon, Lavash and Sangak beside Baguette, were prepared using standard ingredients, specific recipe, and under controlled conditions. After baking and cooling, breads were packed in bi layer PE, in order to minimize the interchanges of moisture and air of products with atmosphere. Each bread was then taken out from the package at different time intervals and 20 mg sample from its crumb was weighted. In next step, thermal properties such as: enthalpy, maximum and final temperature of reactions were determined by Simultaneous Thermal Analysis (STA) method. This evaluation was performed in triplicate during 4 days storage. A factorial experiment in completely randomized design was used for statistical analysis. The results shown that the fresh breads have exothermic curves. However with increasing the storage time, curve becomes more endothermic, and these changes was not similar in all breads. Generally, according to the statistical analysis, it was found that enthalpy is not suitable for evaluation of extent of staling and shelf life in Taftoon, Lavash and Sangak. However, these changes of enthalpy had a regular and documentary trend in Barbari during first three days, althoush, using the enthalpy as parameter for predicting the extent of staling in Barbari still requires more investigation.
KEYWORDS: Barbari, Sangak, Lavash, Taftoon, Enthalpy, Simultaneous Thermal Analysis, Staling.
Mohsen Ghods rohani; Seyed Ali Mortazavi; Seyed Mohammad Ali Razavi; Mostafa Mazaheri Tehrani
Abstract
In this study, soymilk curd (tofu) was produced and evaluated at three stages according to Iranian preference. In the first stage, the effect of soybean varieties (Williams & Habbit), production methods (Traditional & Recommended), and formulation (0, 10 and 20% cheese whey instead of water used in soymilk ...
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In this study, soymilk curd (tofu) was produced and evaluated at three stages according to Iranian preference. In the first stage, the effect of soybean varieties (Williams & Habbit), production methods (Traditional & Recommended), and formulation (0, 10 and 20% cheese whey instead of water used in soymilk production) on the qualitative and quantitative characteristics of tofu were evaluated.
In the second stage, fresh tofu was stored in brine or cheese whey containing salt at different concentrations (2, 4 & 6%) and the quality of tofu was then evaluated. In the third stage, the consumer preference and the consumption attributes were determined. The results shown that the best quality of tofu will be achieved when the Williams variety, the recommended method and 0% whey are used. Furthermore, the results indicated that the most appropriate storage medium is cheese whey with 4% salt. Such product can gain the 72% of the consumer’s acceptability.
Key words: soybean, soymilk, soymilk curd (tofu) and whey.
Mohammad Reza Edalatian Dovom; Manuchehr Hamedi; Seyed Ali Mortazavi; Mostafa Mazaheri Tehrani
Abstract
Tomato is one of the most important crops with a yeild of 98. 8 million tons in more than 128,000 hectare of area under cultivation and 3.7 million tons production in Iran. With regard to technological advancement ( improvement, development ) and increasing demand of tomato and their products in Iran ...
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Tomato is one of the most important crops with a yeild of 98. 8 million tons in more than 128,000 hectare of area under cultivation and 3.7 million tons production in Iran. With regard to technological advancement ( improvement, development ) and increasing demand of tomato and their products in Iran and all over the world, it is necessary to know about affecting factors on production, process and storage of this product more precisely. In recent years, many researches have been performed to study physico – chemical and sensory characteristics of tomato in order to forecast necessary parameters in cultivating and selecting of the tomato variety corresponding to their own application ( fresh eating, processing and both of them ). In this research, the effect of variety and storage on physical properties of tomato paste produced from four tomato varieties (Cal .J.n.3 , Early Urbana Y , Early Urbana111 and Peto early C.H) has been determined in one year storage under ambient temperature (average temperature 25 ºC ). The analysis of data on physical properties including :%PWR,RF Index, consistency and color showed that ,Early UrbanaY had the highest consistency , PWR and total scoring for all of the properties Early Urbana Y totally was the best. Cal.j.n.3 variety in the most physical properties had the least changes during storage and from this point of view it was the most stable variety during storage of tomato paste.
Key words:Tomato paste,variety,storage time,physical properties
Massoumeh Mehraban Sangatash; Hashem Pourazerang; Seyed Ali Mortazavi; Abdolmajid Maskooki; Ashraf Gohari Ardabili
Abstract
The effect of thyme and ajowan essential oils (200µg/l and 300µg/l respectivilly ) on growth of aflatoxin-producing strain of Aspergillus parasiticus on 2 stages of pistachio (green and with dry peel ) was studied . Also Organoleptic tests for evaluating odor and flavor residual of essential oils on ...
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The effect of thyme and ajowan essential oils (200µg/l and 300µg/l respectivilly ) on growth of aflatoxin-producing strain of Aspergillus parasiticus on 2 stages of pistachio (green and with dry peel ) was studied . Also Organoleptic tests for evaluating odor and flavor residual of essential oils on pistachio were also performed tested . The Collected data were statistically analyzed. The results showed that thyme essential oils at 200 µg/l had stronger effect than ajowan essential oils at 300 µg/L in terms of control the growth of Aspergillus parasiticus inspite of more thymol content of ajowan which might be as a resalts of synergistic effect between thymol and other phenolic compounds such as carvacrol in thyme. evaluation of organoleptic by 13 panelists showed that the sample which was treated by ajowan oil scored as more acceptable than the others. It was concluded that both thyme ajowan essential oils are promising natural fungistatical agents and can be used instead of currently used synthetic fungicides.
Keyword : essential oil , thyme , ajowan , Aspergillus parasiticus , pistachio
Masoud Yavarmanesh; Seyed Ali Mortazavi; Mehdi Nasiri mahalati; Javad Barooei
Abstract
Food borne viral infections are recognized increasingly as human illneses. Hepatitis A virus (HAV) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk . In this study for approaching to best diagnosis method of HAV in milk by ELISA technique, ...
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Food borne viral infections are recognized increasingly as human illneses. Hepatitis A virus (HAV) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk . In this study for approaching to best diagnosis method of HAV in milk by ELISA technique, various dilutions of HAV antigen (1 ml/L, 10-3 ml/L, 10-6 ml/L, 10-9 ml/L) were added to UHT milk . Various treatments for virus(antigen) isolation, showed that milk with acidic coagulation, following filtration with paper and membrane filters, had the most optical density. It`s indicated that this treatment resulted the best diagnosis . Also it has been revealed that this method has not any sensitivity for quantitative determination of HAV(antigen) in milk.
Key words: diagnosis, Hepatitis A virus(HAV), Milk, ELISA
