Efficiency of Microwave Radiation on Antioxidant Compounds Extracted from Red Algae “Hypneahamulosa“and Optimal Extraction Conditions Using Response Surface Methodology (RSM)

Document Type : Research Article

Authors

• Elham Garmsiri
• Masoud Rezaei ¹
• Amirreza Shaviklo ²
• Aria Babakhani ¹

¹ Tarbiat Modares University

² Department of Animal Processing, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

https://doi.org/10.22067/ifstrj.v10i2.37820

Abstract

Seaweeds are the novel bioactive compounds resources with antioxidant activity. The aim of this study was to evaluate the efficacy of microwave radiation on antioxidant compounds extracted from red algae Hypneahamulosa and optimal extraction conditions using response surface methodology (RSM).. Independent variables were time (4,8,12 min), acetone/water concentration (50%,75% ,100% acetone) and power microwave (200,300.400 W). Antioxidant activity of red algae was measured using DPPH radical scavenging activity assay.DPPH value varied from 4.19 to 72.99%. The optimal extraction conditions was include acetone concentration of 50%, at 12 min and microwave power of 200 W.The actual experimental values were in close agreement with the predicted values from the developed quadratic polynomial equation .R-squared was obtained99% that shows suitability of the model useded and the success of RSM in optimizing the extraction conditions.

Keywords

• DPPH radical scavenging activity
• Hypneahamulosa
• Microwave-assisted extraction
• Phenolic compounds
• Response surface methodology