بررسی خواص آنتی‌اکسیدانی و آنتی‌باکتریالی عسل زنبور عسل کوچک Apis florea Fabricius (Hymenoptera: Apidae) علیه Helicobacter pylori

نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

• شبنم پری چهره ¹
• غلامحسین طهماسبی ¹
• محمد اسلام پناه ²
• پژواک خاکی ²

¹ بخش زنبور عسل، موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

² بخش میکروب‌شناسی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

https://doi.org/10.22067/ifstrj.2023.81933.1248

چکیده

هلیکوباکتر پیلوری با آلوده‌سازی دستگاه گوارش فوقانی، منجر به آسیب‌دیدگی در مخاط شده و امکان ابتلا به سرطان لوله گوارش را بویژه در مردان افزایش می‌دهد. با توجه به اینکه امکان درمان آن با روش‌های سنتی همیشه به طور موثری امکان‌پذیر نیست، بنابراین تا زمان تهیه واکسن علیه آن، یافتن روش‌های ایمن تر مبارزه با این باکتری بسیار حائز اهمیت است. عسل یک مکمل غذایی با محتوای کربوهیدرات بالا و فعالیت آنتی‌اکسیدانی و همچنین طیف وسیع ضد میکروبی است که در سال‌های اخیر به عنوان یکی از روش‌های مقابله با طیف گسترده‌ای از عوامل میکروبی از جملهH. pylori‎  مطرح بوده است. در این پژوهش برای نخستین بار خواص آنتی‌اکسیدانی و آنتی‌باکتریالی 15 نمونه عسل زنبور عسل کوچک A. florea جمع‌آوری شده از بوشهر، دزفول، ایرانشهر، چابهار، رودان، جهرم و جیرفت مورد بررسی قرار گرفت. ترکیبات فنلی، فلاونوئیدی، پروتئین و ترکیبات معدنی عسل‌های جمع‌آوری شده مورد بررسی قرار گرفت. همچنین خواص آنتی‌اکسیدانی عسل‌ها با استفاده از سه روش DPPH، FRAP و ABTS و خواص ضدباکتریایی در شرایط درون‌تنی و برون‌تنی ارزیابی شد. نتایج نشان داد که همبستگی مثبت و معنی‌داری بین خواص آنتی‌اکسیدانی و آنتی‌باکتریالی عسل‌ها وجود دارد. همچنین براساس نتایج بدست آمده بیشترین خواص آنتی‌باکتریالی علیه هلیکوباکتر پیلوری مربوط به عسل کنار بوشهر بود. طبق نتایج بدست آمده علت تفاوت قدرت آنتی‌اکسیدانی و ضد میکروبی مشاهده شده در بین نمونه‌های عسل به دلیل تفاوت در تنوع فلور گیاهی منطقه و تفاوت جغرافیایی مناطق جمع‌آوری عسل بوده است. بررسی قدرت ضد میکروبی در این پژوهش نشان داد، عسل توانایی بالایی در جلوگیری از آلودگی و نیز درمان آلودگی و التهاب ایجاد شده در دستگاه گوارش به وسیله باکتری هلیکوباکتر پیلوری را داشته است و می‌تواند در کنار سایر روش‌های موجود، در درمان آلودگی به این باکتری مورد استفاده قرار گیرد.

کلیدواژه‌ها

• خواص آنتی اکسیدانی
• خواص ضد باکتریایی
• عسل
• H. pylori

موضوعات

• زیست فناوری مواد غذایی

عنوان مقاله [English]

Evaluation of Antioxidant and Antibacterial Activities of Apis florea Fabricius (Hymenoptera: Apidae) Honey on Helicobacter pylori

نویسندگان [English]

• Shabnam Parichehreh ¹
• Gholamhossein Tahmasbi ¹
• Mohammad Eslampanah ²
• Pejvak Khaki ²

¹ Department of Honeybee, Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

² Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

چکیده [English]

Iranian men are at risk of developing gastrointestinal cancer caused by H. pylori. It is very imperative to find effective methods to control this bacterium as there are currently no very effective treatments for it. Honey has been shown to have antimicrobial properties against various pathogens. This study analyzed 15 honey samples from A. florea bees, collected from different floral and geographical origins, for their antimicrobial efficacy against H. pylori. Using atomic absorption measurements, the honey samples were also tested for their phenolic and flavonoid content, protein concentration, and mineral content. Antioxidant activity was determined using the FRAP, DPPH, and ABTS methods. The antibacterial activity of honey samples was investigated both in-vitro and in-vivo in the gastrointestinal tract of mice. Statistical analysis revealed a significant positive correlation between antioxidant activity and antibacterial activity. All honey samples showed antimicrobial activity in-vitro, among which jujube honey from Bushehr exhibiting the highest activity. Differences in antioxidant and antimicrobial activities were likely due to the flora of the plants and the geographic region from which the honey was harvested. Based on these results, A. florea honey may be used in the prevention and treatment of H. pylori-associated infections and inflammation of the gastrointestinal tract. This feature can be applied to the control of Helicobacter pylori along with other available measures. 

کلیدواژه‌ها [English]

• Antioxidant activity
• Antimicrobial activities
• Honey
• H. pylori

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