نوع مقاله : مقاله پژوهشی

نویسندگان

دانشگاه کشاورزی و منابع طبیعی گرگان

چکیده

بسته‌بندی ضدمیکروبی یک نوع بسته‌بندی فعال است که مهاجرت پیوسته ترکیبات ضدمیکروبی به سطح ماده غذایی را فراهم می‌سازد. در این تحقیق خاصیت ضدمیکروبی اسانس اکالیپتوس گلوبولوس علیه دو باکتری گرم مثبت (استافیلوکوکوس اورئوس و باسیلوس سرئوس) وگرم منفی (اشرشیا کلای و سالمونلا اینترتیدیس) با روش‌های دیسک دیفیوژن ، چاهک میکروپلیت و همچنین تاثیر اسانس اکالیپتوس در محیط مایع و بخارات آن بررسی شد. فیلم زیست کامپوزیت کیتوزان حاوی اسانس اکالیپتوس گلوبولوس (در غلظت‌های 5/0، 1 و 5/1 درصد حجمی/ حجمی) با روش قالب‌گیری تولید و فعالیت ضدمیکروبی آن‌ها در فاز بخار و در تماس مستقیم با محیط مایع مورد ارزیابی قرار گرفت. حداقل غلظت بازدارندگی اسانس علیه باکتری-های گرم منفی و مثبت به‌ترتیب 125/3 و 562/1 میکروگرم بر میلی‌لیتر بود. هم‌چنین قطر هاله بازدارندگی باکتری‌های گرم مثبت از گرم منفی بیشتر بود. ترتیب اثر اسانس روی باکتری‌ها بر اساس اندیس لگاریتم کاهشی به‌صورت: استافیلوکوکوس اورئوس › باسیلوس سرئوس › اشرشیا کلای › سالمونلا اینترتیدیس بود. بخار اسانس در ترکیب با فیلم کیتوزان در سطح 1 و 5/1 درصد اسانس قادر به کاهش دانسیته میکروبی بود و همچنین اندیس لگاریتم کاهشی فیلم کیتوزان با افزایش غلظت اسانس از 5/0 به 5/1 درصد در محیط مایع افزایش یافت. بر اساس این نتایج فیلم زیست کامپوزیت کیتوزان حاوی 5/1 درصد اسانس اکالیپتوس گلوبولوس در تماس مستقیم با مواد غذایی به‌عنوان یک ترکیب ضدمیکروبی مناسب در بسته‌بندی مواد غذایی می‌تواند مورد استفاده قرار گیرد.

کلیدواژه‌ها

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