بهینه‌سازی روش‌های استخراج متداول با حلال فوق داغ جهت استخراج ترکیبات پلی‌فنلی از برگ گیاه نوروزک

نوع مقاله : مقاله پژوهشی انگلیسی

نویسندگان

محبوبه سرابی جماب ¹

الهه منصوری ¹

آرام بستان ²

¹ گروه پژوهشی زیست‌فناوری مواد غذایی، مؤسسه پژوهشی علوم و صنایع غذایی، مشهد، ایران

² گروه پژوهشی نانوفناوری مواد غذایی، مؤسسه پژوهشی علوم و صنایع غذایی، مشهد، ایران

https://doi.org/10.22067/ifstrj.2026.97188.1537

چکیده

کارایی روش استخراج در بازیابی ترکیبات فنلی زیست‌فعال به شدت به مکانیسم و شرایط عملیاتی آن وابسته است. پلی‌فنول‌ها از برگ‌های گیاه نوروزک با استفاده از روش‌های متداول استخراج با حلال و فوق داغ استخراج شدند. طرح آزمایشی مرکب مرکزی از روش آماری سطح پاسخ برای ارزیابی اثرات نسبت حلال، دما و زمان بر بازده فرآیند و محتوای فنلی کل به کار گرفته شد. بهینه‌سازی نشان داد که اصلاح در فرآیند استخراج تا حد زیادی بر راندمان روش استخراج تأثیر می‌گذارد. طبق نتایج، روش استخراج با حلال فوق داغ در مقایسه با روش استخراج متداول با حلال به‌طور قابل توجهی کارآمدتر بود و بالاترین بازده 53.29 درصد و محتوای فنلی کل 737.21 میلی‌گرم معادل اسید گالیک به گرم عصاره استخراج شده تحت شرایط بهینه (25.17 درصد حلال اتانولی در دمای استخراج 160 درجه سانتی­گراد به‌مدت 28.97 دقیقه) به‌دست آمد. در مقابل، بهینه‌سازی روش استخراج متداول با حلال، حداکثر بازده استخراج و فنول کل را به‌ترتیب به 30.96 درصد و 693.23 میلی­گرم معادل اسید گالیک به گرم عصاره استخراج شده (با استفاده از 50 درصد حلال اتانولی در دمای استخراج 82.57 درجه سانتی­گراد به‌مدت 103.31 دقیقه) رساند. این اولین گزارش در مورد بهینه‌سازی استخراج ترکیبات فنولی با حلال فوق داغ از برگ‌ گیاه نوروزک است.

کلیدواژه‌ها

استخراج با حلال فوق داغ

استخراج متداول

پلی‌فنول‌ها

روش سطح پاسخ

نوروزک

موضوعات

• مهندسی مواد غذایی

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عنوان مقاله English

Optimization of Conventional and Superheated Solvent Extraction Methods for Extracting Polyphenolic Compounds from Salvia leriifolia Leaves

نویسندگان English

Mahboobe Sarabi Jamab ¹

Elaheh Mansouri ¹

Aram Bostan ²

¹ Department of Food Biotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

² Department of Food Nanotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

چکیده English

The efficiency of an extraction procedure in recovering bioactive phenolic compounds is highly dependent on its mechanism and operating conditions. Polyphenols were extracted from Salvia leriifolia leaves using conventional solvent extraction and also superheated extraction techniques. The faced central composite experimental design of response surface methodology (RSM) applied to evaluate the effects of the solvent ratio, temperature, and time on both the process yield and total phenolic content. Optimization revealed that the modification in the extraction technology greatly affected the procedure. According to the results, the superheated solvent method was significantly more efficient compared to the conventional solvent extraction, and the highest yield of 53.29 % and total phenolic content of 737.21 mg GAE/g E were obtained under the optimal conditions (25.17 % ethanolic solvent at the extraction temperature of 160 °C for 28.97 min). In contrast, the optimization of conventional solvent extraction method revealed a maximum extraction yield and total phenolics of 30.96 % and 693.23 mg GAE/g E, respectively (using 50 % ethanolic solvent at the extraction temperature of 82.57 °C for 103.31 min). This is the first report on optimizing the superheated solvent extraction of phenolic compounds from Salvia leriifolia leaves.

کلیدواژه‌ها English

Conventional extraction

Polyphenols

Response surface methodology (RSM)

Salvia leriifolia

Superheated solvent extraction

Authors retain the copyright. This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0)

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