نوع مقاله : مقاله پژوهشی فارسی

نویسندگان

گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.

چکیده

در این پژوهش، عصاره گیاه بن‎‏سرخ با استفاده از حلال‌های متانول و آب و به‌کمک روش ماسراسیون استخراج شد. راندمان استخراج، ترکیبات فنول کل، فعالیت آنتی‌اکسیدانی (بر پایه مهار رادیکال‌های 2 و 2-دی‌فنیل 1-پیکریل هیدرازیل (DPPH) و 2،2-آزینو بیس-3-اتیل بنزوتیازولین-6-سولفونیک اسید (ABTS)) و اثر ضدمیکروبی (بر پایه آزمون‏‌های دیسک دیفیوژن آگار، حداقل غلظت مهارکنندگی و حداقل غلظت‌ کشندگی) عصاره‌ها با یکدیگر مقایسه گردید. راندمان استخراج و میزان ترکیبات فنولی عصاره متانولی به‌ترتیب 10/7 درصد و mg GAE/g 28/88 بود، درحالی‌که برای عصاره آبی به‌ترتیب 6/4 درصد و mg GAE/g 29/68 بود. فعالیت آنتی‌اکسیدانی عصاره آبی (برحسب رادیکال­‌های DPPH و ABTS به‌ترتیب mg/mL 28/2 و 98/2) به‌طور قابل‌توجهی بالاتر از عصاره متانولی (برحسب رادیکال‌های DPPH و ABTS به‌ترتیب mg/mL 33/5 و 12/7) بود که نشان می‌دهد وجود ترکیبات فنولی تنها عامل مؤثر بر ظرفیت آنتی‌اکسیدانی عصاره نمی‌باشد. میزان حداقل غلظت مهارکنندگی از رشد عصاره متانولی برای باکتری‌های Streptococcus pyogenes، Listeria innocua، Entrobacter aerogenes، Escherichia coli و Pseudomonas aeruginosa به‌ترتیب 68/4، 68/4، 75، 75/18 و 75/18 میلی‌گرم بر میلی‌لیتر بود، درحالی‌که برای عصاره آبی بن­‌سرخ به‌ترتیب 68/4، 37/9، 75، 5/37 و 5/37 میلی­‌گرم بر میلی‌­لیتر بود. میزان حداقل غلظت کشندگی عصاره متانولی برای باکتری‌­های Streptococcus pyogenes، Listeria innocua، Entrobacter aerogenes، Escherichia coli و Pseudomonas aeruginosa به‌ترتیب 75/18، 75/18، 150، 150 و 5/37 میلی‌گرم بر میلی‌لیتر بود، درحالی‌که برای عصاره آبی بن‌سرخ به‌ترتیب 5/37، 5/37، 300، 150 و 75 میلی‌گرم بر میلی‌لیتر بود. با توجه به نتایج، عصاره گیاه بن‎سرخ قابلیت استفاده به‌عنوان نگهدارنده‏ طبیعی جهت جلوگیری از اکسیداسیون غذاهای حاوی اسیدهای چرب غیراشباع و همچنین کنترل رشد باکتری‌های بیماری‌زا و مولد فساد را دارا می‌باشد. 

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