نوع مقاله : مقاله پژوهشی
نویسندگان
گروه علوم و صنایع غذایی، واحد ورامین– پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
چکیده
اکسیداسیون چربیها از مهمترین دلیل فساد مواد غذایی به شمار میآید. سرعت واکنش اکسیداسیون را میتوان با اضافه کردن آنتیاکسیدانها به تأخیر انداخت. در این مطالعه اثر آنتیاکسیدانی عصاره اتانولی آلوئهورا بر پایداری روغن سویا مورد مطالعه قرار گرفت. ترکیبات موجود در عصاره آلوئه ورا با استفاده از GC/MS شناسایی شدند. بررسی خاصیت آنتیاکسیدانی با اندازهگیری مقدار ترکیلات فنلی و فلاونوئیدی عصاره اتانولی و آزمون رادیکالکنندگی DPPH صورت گرفت و در ادامه عصاره آلوئهورا در چهار سطح ( ppm500،1000،1500،2000) و آنتیاکسیدان سنتزیBHA ، در سطح 120ppm به روغن سویا اضافه و عدد پراکسید، اسیدیته، تیوباربیتوریک اسید و پایداری اکسایشی به روش رنسیمت اندازهگیری شد. نتایج نشان داد با افزایش غلظت عصارههای آلوئهورا از 500 تا 2000 ppm، فساد اکسیداتیو با روند کندتری طی 90 روز نگهداری در روغن سویا مشاهده گردید. پس از 90 روز نگهداری، میزان عدد پراکسید، تیوباربیتوریک اسید و اسیدیته نمونه روغن سویا حاوی ppm 2000 عصاره اتانولی ژل آلوئهورا از نمونه شاهد که حاوی ppm BHA120 بود کمتر بود. میزان فنل کل و مهار رادیکال آزاد و پایداری به فساد اکسیداتیو به روش رنسیمت در نمونه روغن سویا حاوی ppm 2000 عصاره اتانولی ژل آلوئهورا از نمونه روغن سویا که حاوی ppm BHA120 بود بالاتر بود. با توجه به اینکه نمونه روغن سویای حاوی ppm 2000 عصاره اتانولی ژل آلوئهورا میزان فنل کل و مهار رادیکال آزاد و خصوصیات آنتیاکسیدانی بیشتری نسبت به نمونه شاهد داشت بنابراین نمونه مذکور از نظر خواص کیفی و سلامت بخشی به عنوان تیمار برتر انتخاب گردید.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Evaluation of Antioxidant Effect of Ethanolic Extract of Aloe Vera Gel on the Stability of Soybean Oil
نویسندگان [English]
- Marjan Karami
- Leila Nateghi
- Simin Asadollahi
Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
چکیده [English]
Introduction
Oxidation of lipids results in changes that may affect the nutritional quality, wholesomeness, colour, flavour and texture of food. The aim of this study was to investigate the type and amount of phenolic compounds in ethanolic extract of aloe vera gel as a source of natural antioxidant and its effect on the oxidative stability of soybean oil.
Using synthetic antioxidant due to the possibility of toxic and carcinogenic effects is limited. Thus, it is important to find an alternative to synthetic antioxidants by natural antioxidant. Different intrinsic and extrinsic factors may initiate the oxidation of lipids. The initial products of oxidation are tasteless and odorless and after degradation and production of secondary products, the off-flavors and off-odors will appeared in edible oils. This is a great concern in food industry, because it decreases the shelf life of food products. Free radicals are produced during chain reactions in lipid oxidation process. To avoid this, synthetic antioxidants are usually used which are sensible to heat and are hazardous to human health and may cause cancer. Polyphenols have antioxidant activity and absorb free radicals. Thus, the vegetable oils rich in polyphenols can affect human health. In this research, we aimed to investigate the application of natural extract of aloe vera gel as a natural antioxidant to avoid soy oil oxidation and to compare it with synthetic antioxidants.
The rate of oxidation reaction can be delayed by adding antioxidants. Consumers today tend to use natural antioxidants instead of synthetics. The overall purpose of this study was to investigate the effect of adding ethanolic extract of aloe vera gel as natural antioxidant on improving the stability of soybean oil.
Materials and Methods
The compounds in ethanolic extract of aloe vera gel were determined using GC / MS. The antioxidant activity was evaluated by the method of DPPH. For this purpose, ethanolic extracts of aloe vera gel were added to soybean oil in four different concentrations (500, 1000, 1500, 2000 ppm) and peroxide value, acidity, thiobarbituric acid, total phenol, oxidative stability to Rancimat method, fatty acid profile and sensory evaluation were performed on soybean oil samples and compared with the control sample containing 120 ppm BHA and soybean oil sample without adding antioxidants during 30, 60 and 90 days of storage at 25 ° C.
Results and Discussion
The results showed that by increasing the concentration of aloe vera extracts from 500 to 2000 ppm, the oxidation rate decreases during 90 days of storage, the amount of peroxide, thiobarbituric acid and acidity of soybean oil containing 2000 ppm ethanolic extract of aloe vera gel was lower than the control sample containing 120 ppm BHA. The total phenol content and free radical scavenging and stability to oxidative degradation by Rancimat method in soybean oil sample containing 2000 ppm aloe vera ethanol extract was higher than soybean oil samples containing BHA120 ppm. Evaluation of sensory properties showed that no significant difference was observed between the sensory properties of the oil sample containing 2000 ppm ethanolic extract of aloe vera gel and the control soybean oil sample of BHA120 ppm.
Conclusion
Considering that the sample of soybean oil containing 2000 ppm ethanolic extract of aloe vera gel had higher phenol content and free radical scavenging and more antioxidant properties than the control sample, it did not differ significantly from the control sample. Qualitative and health properties were selected as the superior treatment. The results of this study showed that the ethanolic extract of aloe vera gel can be used as a natural antioxidant instead of conventional synthetic antioxidants in the oil industry and to prevent oxidative spoilage of the oil in a desirable way. Therefore, it might be employed as a natural antioxidant in foods, particularly those containing edible oils.
کلیدواژهها [English]
- Aloe vera gel
- Antioxidant activity Soybean oil
©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source. |
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