نوع مقاله : مقاله پژوهشی

نویسندگان

چکیده

هیدرولیز آنزیمی ماهی تون زرد باله (Thunnus Albacares ) با بکارگیری آنزیم نیوتراز توسط روش RSM 6 با استفاده از طرح فاکتوریل (CCD) مورد مطالعه قرار گرفت. طول زنجیره پپتیدی (PCL)7 به عنوان سطح پاسخ به شرایط هیدرولیز (فعالیت آنزیمی، دما، زمان) برآورد شد. .ضریب رگرسیون 84/0=2 Rبرای طول زنجیره پپتیدی در مدل ریاضی پیش‌بینی کرد که 84 درصد تغییر در میزان مطالعه شده به‌وسیله مدل قابل توضیح است. فعالیت آنزیم Au/Kg 37، دما 0C 50 و زمان هیدرولیز 130 دقیقه به عنوان شرایط مطلوب به‌دست آمد. هیدرولیز پروتئین ماهی تون زرد باله میزان پروتئین بالا (56/74 درصد) و میزان لیپید کمی (86/1 درصد) داشته است. شاخص شیمیایی پروتئین هیدرولیز شده نشان می‌دهد که آن نیازهای تغذیه‌ای بچه‌ها در سن 12-10 سال را به غیر از لیزین و متیونین پوشش می‌دهد و لیزین و متیونین به‌ترتیب اولین و دومین اسید آمینه محدودکننده بودند. ولیزین با توجه به نیاز بچه 5-2سال اسید آمینه غالب بود. الگوی الکتروفورتیک نشان می‌دهد، وزن مولکولی پروتئین هیدرولیزشده کمتر از 10 کیلو دالتون است. می‌توان نتیجه گرفت با هیدرولیز آنزیمی امعاء و احشاء ماهی تون زردباله ویژگی‌های پروتئین هیدرولیز شده بهبود بخشیده می‌شود.

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