The aim of this work was to study the antimicrobial activity of tomato skin lycopene oleoresin against Pseudomonas aeruginosa، Escherichia coli، Staphylococcus ureuse، Salmonella typh ، L. monocytogenes ، Bacillus cereus، Bacillus licheniformis. Oleoresin was extracted from tomato peel. Lycopene content was measured by spectrophotometer. Lycopene oleoresin contained 2321mg lycopene/ 100 g oleoresin diluted in serial micro-dilution technique from 40,000 to 78.125 ppm. Microbial culturing was done in ELISA 96-well micro-titer plates in triplet and then MIC and MBC were determined. The results were shown that tomato peel oleoresin contained 2% lycopene, can inhibit and restrain the gram positive and negative bacteria.