جداسازی و شناسایی ترکیبات دارای خواص آنتی‌اکسیدانی زیره سبز (Cuminum cyminum)

نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

• سودابه عین افشار ¹
• هاشم پورآذرنگ ²
• رضا فرهوش ²
• جواد اصیلی ³

¹ مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی

² گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

³ دانشگاه علوم پزشکی مشهد

https://doi.org/10.22067/ifstrj.v1395i0.53312

چکیده

مواد طبیعی ترکیبات پیچیده‌ای هستند که استخراج سریع مواد فعال آنتی‌اکسیدانی از اهمیت خاصی برخوردار است. برای جداسازی چهار جزء  (F1، F2، F3 و F4) موجود در عصاره متانولی زیره سبز از حلال‌هایی با قطبیت متفاوت استفاده شد. خواص آنتی‌اکسیدانی اجزاء فوق، با استفاده از آزمون تعیین قدرت‌گیرندگی رادیکال و آزمون FRAP  تعیین شد. جزء F3 با (میلی‌­گرم بر میلی‌لیتر) 006/0IC50= و (میلی‌مول آهن 2+ در لیتر) 95 /521 FRAP= فعال‌ترین جزء بود. با استفاده ازکروماتوگرافی لایه نازک (TLC) و ارزیابی قدرت‌ گیرندگی رادیکال آزاد (DPPH) دو ترکیب دارای خاصیت آنتی‌اکسیدانی از جزءF3 استخراج شد. به‌منظور تعیین ترکیب شیمیایی این دو جزء با استفاده از کروماتوگرافی تهیه‌ای (HPLC preparative) به‌همراه فاز متحرک  مناسب (استونیتریل: متانول/ آب)  مقدار لازم از این دو ترکیب استخراج شد و با استفاده از HNMR و  CNMR و مقایسه با منابع موجود، دو ترکیب لوتئولین 7 گلوکوزید و آپیژنین 7 گلوکوزید  شناسایی گردیدند.

کلیدواژه‌ها

• آنتی‌اکسیدان
• آپیژنین
• زیره سبز
• لوتئولین

عنوان مقاله [English]

Isolation and identification of antioxidants components From Cumin seed (Cuminum cyminum)

نویسندگان [English]

• Soudabeh Einafshar ¹
• Hashem Pourazerang ²
• Reza Farhoosh ²
• Javad Asili ³

¹ Khorasan Razavi Agricultural and Natural Resources Research and Education Center

² Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran.

³ Department of pharmacognosy

چکیده [English]

Natural materials are complicated compositions; therefore, a rapid screening of the active antioxidants is being challenged in the literature. Different polarity solvents were applied to isolate four fractions (F1, F2, F3 and F4) of methanolic extract of Cuminum cyminum. Their anti-oxidative properties were tested using radical scavenging and FRAP assays. F3 (with IC50=0.006mg/mL and FRAP= 521.95 mmolFe2+/L) was significantly the most active fraction. Guided isolation through bio-autography on TLC using 1, 1- diphenyl- 2 – picryl- hydrazyl radical (DPPH) as a detection reagent led to the isolation of two antioxidant compounds from F3. F3 was injected to a preparative HPLC with the proper mobile phase (acetonitril: methanol/ water) and isolated two main compounds. These compounds were identified as Luteolin 7 glugoside and Apigenin 7 glucoside by means of 1HNMR and 13CNMR and compare them with references.

کلیدواژه‌ها [English]

• Antioxidant
• Apigenin
• Cuminum cyminum
• Luteolin

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